Restriction map for
pFN21AB4983
(Circular) (Six-base) MAPSORT of: pFN21AB4983.seq Check: 2849 from: 1 to: 6232
pFN21AB4983 6232 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4042 278
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3575 1937
Size: 1638 4594
Acc65I G'GTAC_C
Cuts at: 3553 3553
Size: 6232
AclI AA'CG_TT
Cuts at: 1563 4359 4732 1563
Size: 2796 373 3063
Fragments arranged by size:
3063 2796 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2928 3216 4295 5679 5947 1632
Size: 1296 288 1079 1384 268 1917
Fragments arranged by size:
1917 1384 1296 1079 288 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6035
AflIII A'CryG_T
Cuts at: 1203 5131 6108 1203
Size: 3928 977 1327
Fragments arranged by size:
3928 1327 977
AgeI A'CCGG_T
Cuts at: 5063 5063
Size: 6232
AhdI GACnn_n'nnGTC
Cuts at: 4961 4961
Size: 6232
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6232
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6232
AlwNI CAG_nnn'CTG
Cuts at: 3383 3975 5547 3383
Size: 592 1572 4068
Fragments arranged by size:
4068 1572 592
ApaLI G'TGCA_C
Cuts at: 3149 4289 5445 3149
Size: 1140 1156 3936
Fragments arranged by size:
3936 1156 1140
ApoI r'AATT_y
Cuts at: 1493 2359 2732 2816 2965 3012 3042 3263 3541
Size: 866 373 84 149 47 30 221 278
Cuts at: 3541 3767 3919 6028 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 866 373 278 226 221 152
149 84 47 30
AseI AT'TA_AT
Cuts at: 160 1032 4786 160
Size: 872 3754 1606
Fragments arranged by size:
3754 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
AvaI C'yCGr_G
Cuts at: 1870 1943 3557 1870
Size: 73 1614 4545
Fragments arranged by size:
4545 1614 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2589 2622 2589
Size: 33 6199
BamHI G'GATC_C
Cuts at: 3562 3929 3562
Size: 367 5865
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3553 5008 618
Size: 325 217 627 1766 1455 1842
Fragments arranged by size:
1842 1766 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3551 1391
Size: 2160 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5261
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4423 4457 1903
Size: 34 50 34 2402 34 3678
Fragments arranged by size:
3678 2402 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4124 5334 4124
Size: 1210 5022
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6232
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4843 136
Size: 107 122 71 631 605 3171 1525
Fragments arranged by size:
3171 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6227 6227
Size: 6232
BlpI GC'TnA_GC
Cuts at: 3649 3649
Size: 6232
Bme1580I G_kGCm'C
Cuts at: 1790 3153 4293 5449 1790
Size: 1363 1140 1156 2573
Fragments arranged by size:
2573 1363 1156 1140
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6232
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2182 3365 3963
Size: 416 53 83 399 24 765 1183 598
Cuts at: 3963 4921 442
Size: 958 1753
Fragments arranged by size:
1753 1183 958 765 598 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2035 2178 3405 4892 1239
Size: 796 143 1227 1487 2579
Fragments arranged by size:
2579 1487 1227 796 143
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4357 5222 5520 4357
Size: 865 298 5069
Fragments arranged by size:
5069 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4895 882
Size: 617 3396 2219
Fragments arranged by size:
3396 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4023 493
Size: 711 2819 2702
Fragments arranged by size:
2819 2702 711
BsaBI GATnn'nnATC
Cuts at: 6226 6226
Size: 6232
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4039 4421 5056 275
Size: 53 83 186 936 2506 382 635 1451
Fragments arranged by size:
2506 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4665 5063 5337 5484 5895 4665
Size: 398 274 147 411 5002
Fragments arranged by size:
5002 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2151 2181 3378 3408 1178
Size: 30 165 30 748 30 1197 30 4002
Fragments arranged by size:
4002 1197 748 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6232
BseYI C'CCAG_C
Cuts at: 2983 5435 5931 2983
Size: 2452 496 3284
Fragments arranged by size:
3284 2452 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2685 2733 1163
Size: 573 949 48 4662
Fragments arranged by size:
4662 949 573 48
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3682 4443 5471 1540
Size: 275 186 1681 761 1028 2301
Fragments arranged by size:
2301 1681 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3153 3364 4293 4378 5449 3153
Size: 211 929 85 1071 3936
Fragments arranged by size:
3936 1071 929 211 85
BsmI GAATG_Cn'
Cuts at: 3743 3836 3743
Size: 93 6139
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3153 3364 3551 4293 4378 5449 1391
Size: 399 1363 211 187 742 85 1071 2174
Fragments arranged by size:
2174 1363 1071 742 399 211 187 85
BspEI T'CCGG_A
Cuts at: 5895 5895
Size: 6232
BspHI T'CATG_A
Cuts at: 4121 5851 4121
Size: 1730 4502
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3587 844
Size: 2743 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4119 1394
Size: 429 2296 3507
Fragments arranged by size:
3507 2296 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4727 4901 65
Size: 1183 3479 174 1396
Fragments arranged by size:
3479 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4876 5063 1700
Size: 110 3066 187 2869
Fragments arranged by size:
3066 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6232
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6232
BssSI C'ACGA_G
Cuts at: 4292 5304 4292
Size: 1012 5220
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3500 794
Size: 2706 3526
BstBI TT'CG_AA
Cuts at: 5036 5036
Size: 6232
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6232
BstYI r'GATC_y
Cuts at: 1972 2998 3324 3562 3929 4314 4331 5783 5869
Size: 1026 326 238 367 385 17 1452 86
Cuts at: 5869 5881 6227 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1026 385 367 346 326 238
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2308 2550 513
Size: 552 1243 242 4195
Fragments arranged by size:
4195 1243 552 242
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5493
Fragments arranged by size:
5493 683 56
BtsI GCAGTG_nn'
Cuts at: 2097 2490 3748 4542 4562 2097
Size: 393 1258 794 20 3767
Fragments arranged by size:
3767 1258 794 393 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6232
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6197
DraI TTT'AAA
Cuts at: 2632 2815 2821 3536 2632
Size: 183 6 715 5328
Fragments arranged by size:
5328 715 183 6
DraIII CAC_nnn'GTG
Cuts at: 2234 2234
Size: 6232
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5239 809
Size: 507 3923 1802
Fragments arranged by size:
3923 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2944 3679
Size: 54 1425 50 275 18 207 907 735
Cuts at: 3679 4568 6197 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 907 889 735 275 207 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3679 1537
Size: 275 1867 4090
Fragments arranged by size:
4090 1867 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2378 2973 3216 3231 4162 1353
Size: 1025 595 243 15 931 3423
Fragments arranged by size:
3423 1025 931 595 243 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4819 5193 5339 387
Size: 1064 138 3230 374 146 1280
Fragments arranged by size:
3230 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2035 2178 2928 3216 3405 4295 4892
Size: 393 403 143 750 288 189 890 597
Cuts at: 4892 5679 5947 1239
Size: 787 268 1524
Fragments arranged by size:
1524 890 787 750 597 403 393 288
268 189 143
EcoRI G'AATT_C
Cuts at: 3012 3541 3919 3012
Size: 529 378 5325
Fragments arranged by size:
5325 529 378
EcoRV GAT'ATC
Cuts at: 2775 2775
Size: 6232
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3425 3515 3425
Size: 90 6142
FspI TGC'GCA
Cuts at: 4738 4738
Size: 6232
HaeII r_GCGC'y
Cuts at: 1678 5379 1678
Size: 3701 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2002 2034 2309 2341 4873
Size: 32 135 32 430 32 275 32 2532
Cuts at: 4873 4905 4947 4979 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2532 430 275 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3576 3822 669
Size: 988 281 1638 246 3079
Fragments arranged by size:
3079 1638 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2400 3480 748
Size: 1652 1080 3500
Fragments arranged by size:
3500 1652 1080
HpaI GTT'AAC
Cuts at: 3822 3822
Size: 6232
KpnI G_GTAC'C
Cuts at: 3557 3557
Size: 6232
MfeI C'AATT_G
Cuts at: 3163 3831 3163
Size: 668 5564
MluI A'CGCG_T
Cuts at: 6108 6108
Size: 6232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2737 3491 5346 5530 1305
Size: 1432 754 1855 184 2007
Fragments arranged by size:
2007 1855 1432 754 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2039 10
Size: 54 1425 343 207 4203
Fragments arranged by size:
4203 1425 343 207 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2454 2748 4190 4549 4708 518
Size: 648 1288 294 1442 359 159 2042
Fragments arranged by size:
2042 1442 1288 648 359 294 159
MspA1I CmG'CkG
Cuts at: 1606 3648 4325 5473 5718 1606
Size: 2042 677 1148 245 2120
Fragments arranged by size:
2120 2042 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2308 513
Size: 552 1243 4437
Fragments arranged by size:
4437 1243 552
NdeI CA'TA_TG
Cuts at: 387 2301 387
Size: 1914 4318
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6232
NotI GC'GGCC_GC
Cuts at: 3679 3679
Size: 6232
NspI r_CATG'y
Cuts at: 1132 1229 2244 3590 5135 1132
Size: 97 1015 1346 1545 2229
Fragments arranged by size:
2229 1545 1346 1015 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6232
PciI A'CATG_T
Cuts at: 5131 5131
Size: 6232
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3383 1108
Size: 2275 3957
PfoI T'CCnGG_A
Cuts at: 2155 2575 2155
Size: 420 5812
PmeI GTTT'AAAC
Cuts at: 3536 3536
Size: 6232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4301 4333 5814 5846 4301
Size: 32 1481 32 4687
Fragments arranged by size:
4687 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6232
PsiI TTA'TAA
Cuts at: 3802 3802
Size: 6232
PstI C_TGCA'G
Cuts at: 830 2092 2395 3584 830
Size: 1262 303 1189 3478
Fragments arranged by size:
3478 1262 1189 303
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6232
SalI G'TCGA_C
Cuts at: 1936 3574 1936
Size: 1638 4594
SapI GCTCTTCn'nnn_
Cuts at: 2973 3216 2973
Size: 243 5989
SbfI CC_TGCA'GG
Cuts at: 3584 3584
Size: 6232
ScaI AGT'ACT
Cuts at: 2389 3354 4480 6192 2389
Size: 965 1126 1712 2429
Fragments arranged by size:
2429 1712 1126 965
SexAI A'CCwGG_T
Cuts at: 3376 3376
Size: 6232
SfcI C'TryA_G
Cuts at: 826 1045 2075 2088 2391 2613 3580 4715 5396
Size: 219 1030 13 303 222 967 1135 681
Cuts at: 5396 5587 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1030 967 681 303 222 219
191 13
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6232
SmaI CCC'GGG
Cuts at: 1872 3559 1872
Size: 1687 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4336 5237 5499 820
Size: 197 926 2393 901 262 1553
Fragments arranged by size:
2393 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4023 493
Size: 3530 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6232
SphI G_CATG'C
Cuts at: 1132 3590 1132
Size: 2458 3774
SspI AAT'ATT
Cuts at: 5 52 4156 5
Size: 47 4104 2081
Fragments arranged by size:
4104 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6232
StyI C'CwwG_G
Cuts at: 513 1065 2034 2308 3328 3671 513
Size: 552 969 274 1020 343 3074
Fragments arranged by size:
3074 1020 969 552 343 274
SwaI ATTT'AAAT
Cuts at: 2821 2821
Size: 6232
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4259 4276 4429 4614 5059 325
Size: 1255 2679 17 153 185 445 1498
Fragments arranged by size:
2679 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2025 2387 2926
Size: 274 80 33 51 1443 48 362 539
Cuts at: 2926 3249 3352 4478 6190 96
Size: 323 103 1126 1712 138
Fragments arranged by size:
1712 1443 1126 539 362 323 274 138
103 80 51 48 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6192 6224 6192
Size: 32 6200
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6232
XbaI T'CTAG_A
Cuts at: 2327 3568 2327
Size: 1241 4991
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2306 2306
Size: 6232
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6232
XmaI C'CCGG_G
Cuts at: 1870 3557 1870
Size: 1687 4545
XmnI GAAnn'nnTTC
Cuts at: 4361 4361
Size: 6232
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4040 276
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI
SrfI