Restriction map for
pFN21AB4967
(Circular) (Six-base) MAPSORT of: pFN21AB4967.seq Check: 8509 from: 1 to: 11947
pFN21AB4967 11947 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 7019 7094 7780 8753 7019
Size: 75 686 973 10213
Fragments arranged by size:
10213 973 686 75
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 9757 278
Size: 53 83 186 936 8221 2468
Fragments arranged by size:
8221 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5418 6183 7005 8773 9290 1937
Size: 3481 765 822 1768 517 4594
Fragments arranged by size:
4594 3481 1768 822 765 517
Acc65I G'GTAC_C
Cuts at: 9268 9268
Size: 11947
AclI AA'CG_TT
Cuts at: 1563 10074 10447 1563
Size: 8511 373 3063
Fragments arranged by size:
8511 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2022 2180 2657 4187 5085 6498 8783 10010
Size: 390 158 477 1530 898 1413 2285 1227
Cuts at: 10010 11394 11662 1632
Size: 1384 268 1917
Fragments arranged by size:
2285 1917 1530 1413 1384 1227 898 477
390 268 158
AfeI AGC'GCT
Cuts at: 7214 7214
Size: 11947
AflII C'TTAA_G
Cuts at: 820 1017 2729 820
Size: 197 1712 10038
Fragments arranged by size:
10038 1712 197
AflIII A'CryG_T
Cuts at: 1203 3858 9244 10846 11823 1203
Size: 2655 5386 1602 977 1327
Fragments arranged by size:
5386 2655 1602 1327 977
AgeI A'CCGG_T
Cuts at: 10778 10778
Size: 11947
AhdI GACnn_n'nnGTC
Cuts at: 7926 10676 7926
Size: 2750 9197
AleI CACnn'nnGTG
Cuts at: 1166 4886 6620 1166
Size: 3720 1734 6493
Fragments arranged by size:
6493 3720 1734
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 7377 8286 1184
Size: 6193 909 4845
Fragments arranged by size:
6193 4845 909
AlwNI CAG_nnn'CTG
Cuts at: 2888 4589 4772 5176 5527 7228 8138 9690 11262
Size: 1701 183 404 351 1701 910 1552 1572
Cuts at: 11262 2888
Size: 3573
Fragments arranged by size:
3573 1701 1701 1572 1552 910 404 351
183
ApaI G_GGCC'C
Cuts at: 7767 7767
Size: 11947
ApaLI G'TGCA_C
Cuts at: 5551 5841 10004 11160 5551
Size: 290 4163 1156 6338
Fragments arranged by size:
6338 4163 1156 290
ApoI r'AATT_y
Cuts at: 1493 3616 3702 4492 4891 7842 9112 9211 9256
Size: 2123 86 790 399 2951 1270 99 45
Cuts at: 9256 9482 9634 11743 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2951 2123 2109 1697 1270 790 399 226
152 99 86 45
AseI AT'TA_AT
Cuts at: 160 1032 2144 2187 10501 160
Size: 872 1112 43 8314 1606
Fragments arranged by size:
8314 1606 1112 872 43
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11947
AvaI C'yCGr_G
Cuts at: 1870 1943 6716 9272 1870
Size: 73 4773 2556 4545
Fragments arranged by size:
4773 4545 2556 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5479 5512 7063 7096 5479
Size: 33 1551 33 10330
Fragments arranged by size:
10330 1551 33 33
BamHI G'GATC_C
Cuts at: 3790 5209 5722 9277 9644 3790
Size: 1419 513 3555 367 6093
Fragments arranged by size:
6093 3555 1419 513 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5900 7338 7411 8765 9268
Size: 325 217 627 4113 1438 73 1354 503
Cuts at: 9268 10723 618
Size: 1455 1842
Fragments arranged by size:
4113 1842 1455 1438 1354 627 503 325
217 73
BanII G_rGCy'C
Cuts at: 1391 2684 4237 4558 5291 5346 6946 7184 7767
Size: 1293 1553 321 733 55 1600 238 583
Cuts at: 7767 8492 8843 9266 1391
Size: 725 351 423 4072
Fragments arranged by size:
4072 1600 1553 1293 733 725 583 423
351 321 238 55
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3575 5019 5091 6778 928
Size: 971 1676 1444 72 1687 6097
Fragments arranged by size:
6097 1687 1676 1444 971 72
BbvCI CC'TCA_GC
Cuts at: 5247 7629 8007 9003 5247
Size: 2382 378 996 8191
Fragments arranged by size:
8191 2382 996 378
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4727 4761 7951 7985 10138 10172 1903
Size: 34 2790 34 3190 34 2153 34 3678
Fragments arranged by size:
3678 3190 2790 2153 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5480 7217 8231 9839 11049 5480
Size: 1737 1014 1608 1210 6378
Fragments arranged by size:
6378 1737 1608 1210 1014
BclI T'GATC_A
Cuts at: 1548 1548
Size: 11947
BfrBI ATG'CAT
Cuts at: 5767 6133 5767
Size: 366 11581
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2075 2324 3156
Size: 107 122 71 631 605 403 249 832
Cuts at: 3156 4618 8329 10558 136
Size: 1462 3711 2229 1525
Fragments arranged by size:
3711 2229 1525 1462 832 631 605 403
249 122 107 71
BglII A'GATC_T
Cuts at: 5772 11942 5772
Size: 6170 5777
BlpI GC'TnA_GC
Cuts at: 4557 7746 8499 9364 4557
Size: 3189 753 865 7140
Fragments arranged by size:
7140 3189 865 753
Bme1580I G_kGCm'C
Cuts at: 1790 2423 3657 5555 5845 7343 7767 10008 11164
Size: 633 1234 1898 290 1498 424 2241 1156
Cuts at: 11164 1790
Size: 2573
Fragments arranged by size:
2573 2241 1898 1498 1234 1156 633 424
290
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 11947
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5104 5462 6940
Size: 416 53 83 399 24 3687 358 1478
Cuts at: 6940 7926 8866 9678 10636 442
Size: 986 940 812 958 1753
Fragments arranged by size:
3687 1753 1478 986 958 940 812 416
399 358 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 9138 1056
Size: 8082 3865
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6166 6166
Size: 11947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2804 2940 3000 3318 4251 4356 4416 5109
Size: 1565 136 60 318 933 105 60 693
Cuts at: 5109 6941 8008 8450 8962 10607 1239
Size: 1832 1067 442 512 1645 2579
Fragments arranged by size:
2579 1832 1645 1565 1067 933 693 512
442 318 136 105 60 60
Bpu10I CC'TnA_GC
Cuts at: 3362 5247 7629 8007 9003 3362
Size: 1885 2382 378 996 6306
Fragments arranged by size:
6306 2382 1885 996 378
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4365 6250 7659 8572 10072 10937 11235 4365
Size: 1885 1409 913 1500 865 298 5077
Fragments arranged by size:
5077 1885 1500 1409 913 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2822 4443 8187 10610 882
Size: 617 1323 1621 3744 2423 2219
Fragments arranged by size:
3744 2423 2219 1621 1323 617
BsaAI yAC'GTr
Cuts at: 493 1204 7837 9738 493
Size: 711 6633 1901 2702
Fragments arranged by size:
6633 2702 1901 711
BsaBI GATnn'nnATC
Cuts at: 2187 6849 11941 2187
Size: 4662 5092 2193
Fragments arranged by size:
5092 4662 2193
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2078 7607 9754 10136
Size: 53 83 186 936 545 5529 2147 382
Cuts at: 10136 10771 275
Size: 635 1451
Fragments arranged by size:
5529 2147 1451 936 635 545 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5400 10380 10778 11052 11199 11610 5400
Size: 4980 398 274 147 411 5737
Fragments arranged by size:
5737 4980 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3291 3321 7527 7557 1178
Size: 30 165 30 1888 30 4206 30 5568
Fragments arranged by size:
5568 4206 1888 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4188 5093 6190 7725 1186
Size: 3002 905 1097 1535 5408
Fragments arranged by size:
5408 3002 1535 1097 905
BseYI C'CCAG_C
Cuts at: 2721 3386 4167 4464 4734 4827 5664 7611 8013
Size: 665 781 297 270 93 837 1947 402
Cuts at: 8013 8061 8186 8238 8442 8844 8934 8964 11150
Size: 48 125 52 204 402 90 30 2186
Cuts at: 11150 11646 2721
Size: 496 3022
Fragments arranged by size:
3022 2186 1947 837 781 665 496 402
402 297 270 204 125 93 90 52
48 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4698 4715 6962 7319 1163
Size: 573 2962 17 2247 357 5791
Fragments arranged by size:
5791 2962 2247 573 357 17
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 9397 10158 11186 1540
Size: 275 186 7396 761 1028 2301
Fragments arranged by size:
7396 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2127 2986 3304 4237 4763 5555 5845 10008 10093
Size: 859 318 933 526 792 290 4163 85
Cuts at: 10093 11164 2127
Size: 1071 2910
Fragments arranged by size:
4163 2910 1071 933 859 792 526 318
290 85
BsmI GAATG_Cn'
Cuts at: 2518 2904 3604 7025 7258 8252 9458 9551 2518
Size: 386 700 3421 233 994 1206 93 4914
Fragments arranged by size:
4914 3421 1206 994 700 386 233 93
BsmBI CGTCTCn'nnnn_
Cuts at: 7808 8548 7808
Size: 740 11207
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2127 2423 2684 2986 3304 3657 4237
Size: 399 337 296 261 302 318 353 580
Cuts at: 4237 4558 4763 5291 5346 5555 5845 6946 7184
Size: 321 205 528 55 209 290 1101 238
Cuts at: 7184 7343 7767 8492 8843 9266 10008 10093 11164
Size: 159 424 725 351 423 742 85 1071
Cuts at: 11164 1391
Size: 2174
Fragments arranged by size:
2174 1101 1071 742 725 580 528 424
423 399 353 351 337 321 318 302
296 290 261 238 209 205 159 85
55
BspEI T'CCGG_A
Cuts at: 11610 11610
Size: 11947
BspHI T'CATG_A
Cuts at: 2577 6247 9836 11566 2577
Size: 3670 3589 1730 2958
Fragments arranged by size:
3670 3589 2958 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2920 7019 7094 7780 8753 9302 844
Size: 2076 4099 75 686 973 549 3489
Fragments arranged by size:
4099 3489 2076 973 686 549 75
BsrBI CCG'CTC
Cuts at: 1394 1823 3884 9834 1394
Size: 429 2061 5950 3507
Fragments arranged by size:
5950 3507 2061 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2554 4902 6349 6613 6804 7021 7359
Size: 1183 1306 2348 1447 264 191 217 338
Cuts at: 7359 7797 10442 10616 65
Size: 438 2645 174 1396
Fragments arranged by size:
2645 2348 1447 1396 1306 1183 438 338
264 217 191 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 10591 10778 1700
Size: 110 8781 187 2869
Fragments arranged by size:
8781 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3221 5744 96
Size: 3125 2523 6299
Fragments arranged by size:
6299 3125 2523
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11947
BssSI C'ACGA_G
Cuts at: 5562 10007 11019 5562
Size: 4445 1012 6490
Fragments arranged by size:
6490 4445 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3165 3182 3973 4721 7020 7456 8212 8569
Size: 2371 17 791 748 2299 436 756 357
Cuts at: 8569 8683 8695 8704 8854 794
Size: 114 12 9 150 3887
Fragments arranged by size:
3887 2371 2299 791 756 748 436 357
150 114 17 12 9
BstBI TT'CG_AA
Cuts at: 3742 10751 3742
Size: 7009 4938
BstEII G'GTnAC_C
Cuts at: 1184 7717 1184
Size: 6533 5414
BstXI CCAn_nnnn'nTGG
Cuts at: 6541 8654 9079 9181 6541
Size: 2113 425 102 9307
Fragments arranged by size:
9307 2113 425 102
BstYI r'GATC_y
Cuts at: 1972 3736 3790 3921 5209 5296 5625 5722 5772
Size: 1764 54 131 1288 87 329 97 50
Cuts at: 5772 8898 9277 9644 10029 10046 11498 11584 11596
Size: 3126 379 367 385 17 1452 86 12
Cuts at: 11596 11942 1972
Size: 346 1977
Fragments arranged by size:
3126 1977 1764 1452 1288 385 379 367
346 329 131 97 87 86 54 50
17 12
BstZ17I GTA'TAC
Cuts at: 5419 6184 5419
Size: 765 11182
BtgI C'CryG_G
Cuts at: 513 1065 2004 2490 4087 4210 5292 6000 6765
Size: 552 939 486 1597 123 1082 708 765
Cuts at: 6765 7887 7896 8475 8951 9078 9160 9174 513
Size: 1122 9 579 476 127 82 14 3286
Fragments arranged by size:
3286 1597 1122 1082 939 765 708 579
552 486 476 127 123 82 14 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2103 487
Size: 683 56 877 10331
Fragments arranged by size:
10331 877 683 56
BtsI GCAGTG_nn'
Cuts at: 3098 3145 4099 7265 7387 7785 9463 10257 10277
Size: 47 954 3166 122 398 1678 794 20
Cuts at: 10277 3098
Size: 4768
Fragments arranged by size:
4768 3166 1678 954 794 398 122 47
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11947
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 6015 6050 7644 7679 529
Size: 35 5451 35 1594 35 4797
Fragments arranged by size:
5451 4797 1594 35 35 35
DraI TTT'AAA
Cuts at: 3804 5832 6466 9251 3804
Size: 2028 634 2785 6500
Fragments arranged by size:
6500 2785 2028 634
DraIII CAC_nnn'GTG
Cuts at: 3182 6326 6960 7033 3182
Size: 3144 634 73 8096
Fragments arranged by size:
8096 3144 634 73
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3234 7001 8179 10954 809
Size: 507 1918 3767 1178 2775 1802
Fragments arranged by size:
3767 2775 1918 1802 1178 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2316 4150
Size: 54 1425 50 275 18 177 309 1834
Cuts at: 4150 7500 7582 8619 8954 9394 10283 11912 8
Size: 3350 82 1037 335 440 889 1629 43
Fragments arranged by size:
3350 1834 1629 1425 1037 889 440 335
309 275 177 82 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 9394 1537
Size: 275 7582 4090
Fragments arranged by size:
7582 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2676 4206 4488 5037 5279 6573 6934 9877
Size: 1323 1530 282 549 242 1294 361 2943
Cuts at: 9877 1353
Size: 3423
Fragments arranged by size:
3423 2943 1530 1323 1294 549 361 282
242
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2071 6788 10534 10908 11054 387
Size: 1064 138 482 4717 3746 374 146 1280
Fragments arranged by size:
4717 3746 1280 1064 482 374 146 138
EcoICRI GAG'CTC
Cuts at: 4235 4235
Size: 11947
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2022 2180 2657 2804 2940 3000 3318
Size: 393 390 158 477 147 136 60 318
Cuts at: 3318 4187 4251 4356 4416 5085 5109 6498 6941
Size: 869 64 105 60 669 24 1389 443
Cuts at: 6941 8008 8450 8783 8962 10010 10607 11394 11662
Size: 1067 442 333 179 1048 597 787 268
Cuts at: 11662 1239
Size: 1524
Fragments arranged by size:
1524 1389 1067 1048 869 787 669 597
477 443 442 393 390 333 318 268
179 158 147 136 105 64 60 60
24
EcoNI CCTnn'n_nnAGG
Cuts at: 2346 2892 5955 2346
Size: 546 3063 8338
Fragments arranged by size:
8338 3063 546
EcoO109I rG'GnC_Cy
Cuts at: 2441 2717 3665 4019 7540 7763 7764 7928 8609
Size: 276 948 354 3521 223 1 164 681
Cuts at: 8609 8814 2441
Size: 205 5574
Fragments arranged by size:
5574 3521 948 681 354 276 223 205
164 1
EcoRI G'AATT_C
Cuts at: 3702 4492 4891 9112 9211 9256 9634 3702
Size: 790 399 4221 99 45 378 6015
Fragments arranged by size:
6015 4221 790 399 378 99 45
FspI TGC'GCA
Cuts at: 10453 10453
Size: 11947
HaeII r_GCGC'y
Cuts at: 1678 3064 7216 7825 9174 11094 1678
Size: 1386 4152 609 1349 1920 2531
Fragments arranged by size:
4152 2531 1920 1386 1349 609
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2090 2122 2455 2487 2653
Size: 32 135 32 518 32 333 32 166
Cuts at: 2653 2685 3374 3406 3523 3555 4099 4131 5201
Size: 32 689 32 117 32 544 32 1070
Cuts at: 5201 5233 6865 6897 7912 7944 8674 8706 8986
Size: 32 1632 32 1015 32 730 32 280
Cuts at: 8986 9018 10588 10620 10662 10694 1373
Size: 32 1570 32 42 32 2626
Fragments arranged by size:
2626 1632 1570 1070 1015 730 689 544
518 333 280 166 135 117 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2454 2915 3344 5234 6112 8072
Size: 988 281 516 461 429 1890 878 1960
Cuts at: 8072 9291 9537 669
Size: 1219 246 3079
Fragments arranged by size:
3079 1960 1890 1219 988 878 516 461
429 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 11947
HpaI GTT'AAC
Cuts at: 9537 9537
Size: 11947
KpnI G_GTAC'C
Cuts at: 9272 9272
Size: 11947
MfeI C'AATT_G
Cuts at: 2589 7258 9546 2589
Size: 4669 2288 4990
Fragments arranged by size:
4990 4669 2288
MluI A'CGCG_T
Cuts at: 11823 11823
Size: 11947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3289 4305 5487 7229 7592 7751 7885 11061
Size: 1984 1016 1182 1742 363 159 134 3176
Cuts at: 11061 11245 1305
Size: 184 2007
Fragments arranged by size:
3176 2007 1984 1742 1182 1016 363 184
159 134
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2318 4152 7502 7584 8621
Size: 54 1425 343 486 1834 3350 82 1037
Cuts at: 8621 10
Size: 3336
Fragments arranged by size:
3350 3336 1834 1425 1037 486 343 82
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2414 2495 2507 4886 5604 6620 6962
Size: 648 1248 81 12 2379 718 1016 342
Cuts at: 6962 6968 7901 9905 10264 10423 518
Size: 6 933 2004 359 159 2042
Fragments arranged by size:
2379 2042 2004 1248 1016 933 718 648
359 342 159 81 12 6
MspA1I CmG'CkG
Cuts at: 1606 2245 2993 4362 4795 4835 7456 7513 7811
Size: 639 748 1369 433 40 2621 57 298
Cuts at: 7811 8242 8266 8680 9363 10040 11188 11433 1606
Size: 431 24 414 683 677 1148 245 2120
Fragments arranged by size:
2621 2120 1369 1148 748 683 677 639
433 431 414 298 245 57 40 24
NcoI C'CATG_G
Cuts at: 513 1065 2004 2490 4210 5292 6765 7887 7896
Size: 552 939 486 1720 1082 1473 1122 9
Cuts at: 7896 8475 9078 9160 513
Size: 579 603 82 3300
Fragments arranged by size:
3300 1720 1473 1122 1082 939 603 579
552 486 82 9
NdeI CA'TA_TG
Cuts at: 387 3835 8853 387
Size: 3448 5018 3481
Fragments arranged by size:
5018 3481 3448
NheI G'CTAG_C
Cuts at: 1052 9134 1052
Size: 8082 3865
NotI GC'GGCC_GC
Cuts at: 9394 9394
Size: 11947
NsiI A_TGCA'T
Cuts at: 5769 6135 5769
Size: 366 11581
NspI r_CATG'y
Cuts at: 1132 1229 3047 3514 3862 3976 6133 6137 6318
Size: 97 1818 467 348 114 2157 4 181
Cuts at: 6318 6967 7510 7654 8323 8698 9305 10850 1132
Size: 649 543 144 669 375 607 1545 2229
Fragments arranged by size:
2229 2157 1818 1545 669 649 607 543
467 375 348 181 144 114 97 4
PasI CC'CwG_GG
Cuts at: 1348 3661 7696 8306 8326 1348
Size: 2313 4035 610 20 4969
Fragments arranged by size:
4969 4035 2313 610 20
PciI A'CATG_T
Cuts at: 3858 10846 3858
Size: 6988 4959
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2971 4133 7117 7228 8245 8749 9050 1108
Size: 1863 1162 2984 111 1017 504 301 4005
Fragments arranged by size:
4005 2984 1863 1162 1017 504 301 111
PfoI T'CCnGG_A
Cuts at: 2341 2527 2785 5125 9049 2341
Size: 186 258 2340 3924 5239
Fragments arranged by size:
5239 3924 2340 258 186
PmeI GTTT'AAAC
Cuts at: 9251 9251
Size: 11947
PmlI CAC'GTG
Cuts at: 7837 7837
Size: 11947
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10016 10048 11529 11561 10016
Size: 32 1481 32 10402
Fragments arranged by size:
10402 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2441 3665 7928 8814 2441
Size: 1224 4263 886 5574
Fragments arranged by size:
5574 4263 1224 886
PshAI GACnn'nnGTC
Cuts at: 1613 6239 1613
Size: 4626 7321
PsiI TTA'TAA
Cuts at: 9517 9517
Size: 11947
PspOMI G'GGCC_C
Cuts at: 7763 7763
Size: 11947
PstI C_TGCA'G
Cuts at: 830 2545 3991 6241 6396 7171 7461 7777 8202
Size: 1715 1446 2250 155 775 290 316 425
Cuts at: 8202 9299 830
Size: 1097 3478
Fragments arranged by size:
3478 2250 1715 1446 1097 775 425 316
290 155
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11947
PvuII CAG'CTG
Cuts at: 2245 2993 4835 7456 8242 2245
Size: 748 1842 2621 786 5950
Fragments arranged by size:
5950 2621 1842 786 748
SacI G_AGCT'C
Cuts at: 4237 4237
Size: 11947
SalI G'TCGA_C
Cuts at: 1936 9289 1936
Size: 7353 4594
SanDI GG'GwC_CC
Cuts at: 8814 8814
Size: 11947
SapI GCTCTTCn'nnn_
Cuts at: 4488 5279 6934 4488
Size: 791 1655 9501
Fragments arranged by size:
9501 1655 791
SbfI CC_TGCA'GG
Cuts at: 9299 9299
Size: 11947
ScaI AGT'ACT
Cuts at: 5098 10195 11907 5098
Size: 5097 1712 5138
Fragments arranged by size:
5138 5097 1712
SfcI C'TryA_G
Cuts at: 826 1045 2154 2541 3959 3987 6237 6392 7167
Size: 219 1109 387 1418 28 2250 155 775
Cuts at: 7167 7457 7469 7773 8021 8198 8342 8774 9295
Size: 290 12 304 248 177 144 432 521
Cuts at: 9295 10430 11111 11302 826
Size: 1135 681 191 1471
Fragments arranged by size:
2250 1471 1418 1135 1109 775 681 521
432 387 304 290 248 219 191 177
155 144 28 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 2324 2324
Size: 11947
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11947
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11947
SmaI CCC'GGG
Cuts at: 1872 6718 9274 1872
Size: 4846 2556 4545
Fragments arranged by size:
4846 4545 2556
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2729 4380 6229 7674 8551 10051
Size: 197 926 786 1651 1849 1445 877 1500
Cuts at: 10051 10952 11214 820
Size: 901 262 1553
Fragments arranged by size:
1849 1651 1553 1500 1445 926 901 877
786 262 197
SnaBI TAC'GTA
Cuts at: 493 9738 493
Size: 9245 2702
SpeI A'CTAG_T
Cuts at: 152 2423 4874 152
Size: 2271 2451 7225
Fragments arranged by size:
7225 2451 2271
SphI G_CATG'C
Cuts at: 1132 3047 3514 6133 6967 7510 7654 8323 9305
Size: 1915 467 2619 834 543 144 669 982
Cuts at: 9305 1132
Size: 3774
Fragments arranged by size:
3774 2619 1915 982 834 669 543 467
144
SspI AAT'ATT
Cuts at: 5 52 9871 5
Size: 47 9819 2081
Fragments arranged by size:
9819 2081 47
StuI AGG'CCT
Cuts at: 1517 2946 7131 8747 8780 1517
Size: 1429 4185 1616 33 4684
Fragments arranged by size:
4684 4185 1616 1429 33
StyI C'CwwG_G
Cuts at: 513 1065 2004 2490 2812 2965 3505 4210 4649
Size: 552 939 486 322 153 540 705 439
Cuts at: 4649 5292 5482 5629 6627 6765 7132 7308 7887
Size: 643 190 147 998 138 367 176 579
Cuts at: 7887 7896 7993 8036 8475 8647 8748 8990 9078
Size: 9 97 43 439 172 101 242 88
Cuts at: 9078 9160 9386 513
Size: 82 226 3074
Fragments arranged by size:
3074 998 939 705 643 579 552 540
486 439 439 367 322 242 226 190
176 172 153 147 138 101 97 88
82 43 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6181 7190 7975 8488 9974 9991 10144
Size: 1255 4601 1009 785 513 1486 17 153
Cuts at: 10144 10329 10774 325
Size: 185 445 1498
Fragments arranged by size:
4601 1498 1486 1255 1009 785 513 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2839 3221 5096
Size: 274 80 33 51 1443 862 382 1875
Cuts at: 5096 5744 7052 9230 10193 11905 96
Size: 648 1308 2178 963 1712 138
Fragments arranged by size:
2178 1875 1712 1443 1308 963 862 648
382 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5707 5739 8286 8318 9008 9040 11907 11939 5707
Size: 32 2547 32 690 32 2867 32 5715
Fragments arranged by size:
5715 2867 2547 690 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 11947
XbaI T'CTAG_A
Cuts at: 5908 9283 5908
Size: 3375 8572
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2413 4951 6964 7894 7939 8053 2413
Size: 2538 2013 930 45 114 6307
Fragments arranged by size:
6307 2538 2013 930 114 45
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 11947
XmaI C'CCGG_G
Cuts at: 1870 6716 9272 1870
Size: 4846 2556 4545
Fragments arranged by size:
4846 4545 2556
XmnI GAAnn'nnTTC
Cuts at: 4051 5220 5615 6060 10076 4051
Size: 1169 395 445 4016 5922
Fragments arranged by size:
5922 4016 1169 445 395
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 9755 276
Size: 53 83 186 936 8221 2468
Fragments arranged by size:
8221 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BsiWI Bsu36I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PspXI PsrI RsrII SacII SexAI SfoI SrfI SwaI