Restriction map for
pFN21AB4959
(Circular) (Six-base) MAPSORT of: pFN21AB4959.seq Check: 3875 from: 1 to: 6409
pFN21AB4959 6409 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3523 4219 278
Size: 53 83 186 936 1987 696 2468
Fragments arranged by size:
2468 1987 936 696 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3378 3752 1937
Size: 1441 374 4594
Fragments arranged by size:
4594 1441 374
Acc65I G'GTAC_C
Cuts at: 3730 3730
Size: 6409
AclI AA'CG_TT
Cuts at: 1563 4536 4909 1563
Size: 2973 373 3063
Fragments arranged by size:
3063 2973 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2327 2813 3014 4472 5856 6124 1632
Size: 695 486 201 1458 1384 268 1917
Fragments arranged by size:
1917 1458 1384 695 486 268 201
AfeI AGC'GCT
Cuts at: 2711 2711
Size: 6409
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6212
AflIII A'CryG_T
Cuts at: 1203 5308 6285 1203
Size: 4105 977 1327
Fragments arranged by size:
4105 1327 977
AgeI A'CCGG_T
Cuts at: 5240 5240
Size: 6409
AhdI GACnn_n'nnGTC
Cuts at: 5138 5138
Size: 6409
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6409
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6409
AlwNI CAG_nnn'CTG
Cuts at: 2541 2697 3178 3229 4152 5724 2541
Size: 156 481 51 923 1572 3226
Fragments arranged by size:
3226 1572 923 481 156 51
ApaLI G'TGCA_C
Cuts at: 2661 3185 4466 5622 2661
Size: 524 1281 1156 3448
Fragments arranged by size:
3448 1281 1156 524
ApoI r'AATT_y
Cuts at: 1493 2331 3718 3944 4096 6205 1493
Size: 838 1387 226 152 2109 1697
Fragments arranged by size:
2109 1697 1387 838 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4963 160
Size: 872 3931 1606
Fragments arranged by size:
3931 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6409
AvaI C'yCGr_G
Cuts at: 1870 1943 3734 1870
Size: 73 1791 4545
Fragments arranged by size:
4545 1791 73
BamHI G'GATC_C
Cuts at: 3739 4106 3739
Size: 367 6042
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2211 3730 5185 618
Size: 325 217 627 424 1519 1455 1842
Fragments arranged by size:
1842 1519 1455 627 424 325 217
BanII G_rGCy'C
Cuts at: 1391 2252 3728 1391
Size: 861 1476 4072
Fragments arranged by size:
4072 1476 861
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2144 2589 3317 928
Size: 971 245 445 728 4020
Fragments arranged by size:
4020 971 728 445 245
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2642 2676 2794 2828 3110 3144 4600
Size: 34 705 34 118 34 282 34 1456
Cuts at: 4600 4634 1903
Size: 34 3678
Fragments arranged by size:
3678 1456 705 282 118 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3679 4301 5511 3679
Size: 622 1210 4577
Fragments arranged by size:
4577 1210 622
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6409
BfrBI ATG'CAT
Cuts at: 3536 3536
Size: 6409
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2759 3025 5020
Size: 107 122 71 631 605 1087 266 1995
Cuts at: 5020 136
Size: 1525
Fragments arranged by size:
1995 1525 1087 631 605 266 122 107
71
BglII A'GATC_T
Cuts at: 2346 6404 2346
Size: 4058 2351
BlpI GC'TnA_GC
Cuts at: 2246 3158 3826 2246
Size: 912 668 4829
Fragments arranged by size:
4829 912 668
Bme1580I G_kGCm'C
Cuts at: 1790 2214 2665 3189 4470 5626 1790
Size: 424 451 524 1281 1156 2573
Fragments arranged by size:
2573 1281 1156 524 451 424
BmgBI CAC'GTC
Cuts at: 1318 2782 1318
Size: 1464 4945
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3043 3602 4140
Size: 416 53 83 399 24 1626 559 538
Cuts at: 4140 5098 442
Size: 958 1753
Fragments arranged by size:
1753 1626 958 559 538 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6409
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2782 2782
Size: 6409
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3059 3116 3167 3251 3600 5069 1239
Size: 1820 57 51 84 349 1469 2579
Fragments arranged by size:
2579 1820 1469 349 84 57 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2497 4534 5399 5697 2497
Size: 2037 865 298 3209
Fragments arranged by size:
3209 2037 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2487 3227 5072 882
Size: 617 988 740 1845 2219
Fragments arranged by size:
2219 1845 988 740 617
BsaAI yAC'GTr
Cuts at: 493 1204 2500 4200 493
Size: 711 1296 1700 2702
Fragments arranged by size:
2702 1700 1296 711
BsaBI GATnn'nnATC
Cuts at: 3590 6403 3590
Size: 2813 3596
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2090 3520 4216 4598
Size: 53 83 186 936 557 1430 696 382
Cuts at: 4598 5233 275
Size: 635 1451
Fragments arranged by size:
1451 1430 936 696 635 557 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4842 5240 5514 5661 6072 4842
Size: 398 274 147 411 5179
Fragments arranged by size:
5179 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2138 2168 2495 2525 1178
Size: 30 165 30 735 30 327 30 5062
Fragments arranged by size:
5062 735 327 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2662 1186
Size: 1476 4933
BseYI C'CCAG_C
Cuts at: 2084 2392 2690 3153 5612 6108 2084
Size: 308 298 463 2459 496 2385
Fragments arranged by size:
2459 2385 496 463 308 298
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2051 2206 2397 2647 2795 1163
Size: 573 315 155 191 250 148 4777
Fragments arranged by size:
4777 573 315 250 191 155 148
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3859 4620 5648 1540
Size: 275 186 1858 761 1028 2301
Fragments arranged by size:
2301 1858 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2252 2665 3189 3258 4470 4555 5626 2252
Size: 413 524 69 1212 85 1071 3035
Fragments arranged by size:
3035 1212 1071 524 413 85 69
BsmI GAATG_Cn'
Cuts at: 3536 3920 4013 3536
Size: 384 93 5932
Fragments arranged by size:
5932 384 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2214 2252 2665 3189 3258 3728 4470
Size: 399 424 38 413 524 69 470 742
Cuts at: 4470 4555 5626 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 524 470 424 413 399
85 69 38
BspEI T'CCGG_A
Cuts at: 6072 6072
Size: 6409
BspHI T'CATG_A
Cuts at: 4298 6028 4298
Size: 1730 4679
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2496 2796 3222 3764 844
Size: 1652 300 426 542 3489
Fragments arranged by size:
3489 1652 542 426 300
BsrBI CCG'CTC
Cuts at: 1394 1823 4296 1394
Size: 429 2473 3507
Fragments arranged by size:
3507 2473 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4904 5078 65
Size: 1183 3656 174 1396
Fragments arranged by size:
3656 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3162 5053 5240 1700
Size: 110 1352 1891 187 2869
Fragments arranged by size:
2869 1891 1352 187 110
BsrGI T'GTAC_A
Cuts at: 96 2267 96
Size: 2171 4238
BssHII G'CGCG_C
Cuts at: 1925 2037 1925
Size: 112 6297
BssSI C'ACGA_G
Cuts at: 2756 4469 5481 2756
Size: 1713 1012 3684
Fragments arranged by size:
3684 1713 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2389 2541 794
Size: 1595 152 4662
Fragments arranged by size:
4662 1595 152
BstBI TT'CG_AA
Cuts at: 2932 5213 2932
Size: 2281 4128
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6409
BstYI r'GATC_y
Cuts at: 1972 2346 3012 3694 3739 4106 4491 4508 5960
Size: 374 666 682 45 367 385 17 1452
Cuts at: 5960 6046 6058 6404 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 682 666 385 374 367 346
86 45 17 12
Bsu36I CC'TnA_GG
Cuts at: 2645 2645
Size: 6409
BtgI C'CryG_G
Cuts at: 513 1065 2004 2142 513
Size: 552 939 138 4780
Fragments arranged by size:
4780 939 552 138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5670
Fragments arranged by size:
5670 683 56
BtsI GCAGTG_nn'
Cuts at: 2086 2539 3925 4719 4739 2086
Size: 453 1386 794 20 3756
Fragments arranged by size:
3756 1386 794 453 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6409
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6374
DraI TTT'AAA
Cuts at: 2415 3713 2415
Size: 1298 5111
DraIII CAC_nnn'GTG
Cuts at: 2179 2179
Size: 6409
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3415 5416 809
Size: 507 2099 2001 1802
Fragments arranged by size:
2099 2001 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3467 3856 4745
Size: 54 1425 50 275 18 1637 389 889
Cuts at: 4745 6374 8
Size: 1629 43
Fragments arranged by size:
1637 1629 1425 889 389 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3856 1537
Size: 275 2044 4090
Fragments arranged by size:
4090 2044 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2257 2346 3050 4339 1353
Size: 904 89 704 1289 3423
Fragments arranged by size:
3423 1289 904 704 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2758 3185 3301 4996 5370 5516
Size: 1064 138 1169 427 116 1695 374 146
Cuts at: 5516 387
Size: 1280
Fragments arranged by size:
1695 1280 1169 1064 427 374 146 138
116
EcoICRI GAG'CTC
Cuts at: 2250 2250
Size: 6409
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2327 2813 3014 3059 3116 3167 3251
Size: 393 695 486 201 45 57 51 84
Cuts at: 3251 3600 4472 5069 5856 6124 1239
Size: 349 872 597 787 268 1524
Fragments arranged by size:
1524 872 787 695 597 486 393 349
268 201 84 57 51 45
EcoNI CCTnn'n_nnAGG
Cuts at: 2109 2792 2109
Size: 683 5726
EcoO109I rG'GnC_Cy
Cuts at: 3226 3510 3226
Size: 284 6125
EcoRI G'AATT_C
Cuts at: 3718 4096 3718
Size: 378 6031
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2845 2845
Size: 6409
FspI TGC'GCA
Cuts at: 4915 4915
Size: 6409
HaeII r_GCGC'y
Cuts at: 1678 2713 5556 1678
Size: 1035 2843 2531
Fragments arranged by size:
2843 2531 1035
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2014 2046 2338 2370 3243
Size: 32 135 32 442 32 292 32 873
Cuts at: 3243 3275 3494 3526 5050 5082 5124 5156 1373
Size: 32 219 32 1524 32 42 32 2626
Fragments arranged by size:
2626 1524 873 442 292 219 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2785 3753 3999 669
Size: 988 281 847 968 246 3079
Fragments arranged by size:
3079 988 968 847 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6409
HpaI GTT'AAC
Cuts at: 3999 3999
Size: 6409
KpnI G_GTAC'C
Cuts at: 3734 3734
Size: 6409
MfeI C'AATT_G
Cuts at: 4008 4008
Size: 6409
MluI A'CGCG_T
Cuts at: 6285 6285
Size: 6409
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2336 5523 5707 1305
Size: 1031 3187 184 2007
Fragments arranged by size:
3187 2007 1031 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4587
Fragments arranged by size:
4587 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3497 4367 4726 4885 518
Size: 648 2331 870 359 159 2042
Fragments arranged by size:
2331 2042 870 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3157 3825 4502 5650 5895 1606
Size: 1551 668 677 1148 245 2120
Fragments arranged by size:
2120 1551 1148 677 668 245
NaeI GCC'GGC
Cuts at: 3164 3164
Size: 6409
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4918
Fragments arranged by size:
4918 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6409
NgoMIV G'CCGG_C
Cuts at: 3162 3162
Size: 6409
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6409
NotI GC'GGCC_GC
Cuts at: 3856 3856
Size: 6409
NruI TCG'CGA
Cuts at: 3273 3273
Size: 6409
NsiI A_TGCA'T
Cuts at: 3538 3538
Size: 6409
NspI r_CATG'y
Cuts at: 1132 1229 2535 3767 5312 1132
Size: 97 1306 1232 1545 2229
Fragments arranged by size:
2229 1545 1306 1232 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6409
PciI A'CATG_T
Cuts at: 5308 5308
Size: 6409
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3229 1108
Size: 2121 4288
PfoI T'CCnGG_A
Cuts at: 3228 3228
Size: 6409
PmeI GTTT'AAAC
Cuts at: 3713 3713
Size: 6409
PmlI CAC'GTG
Cuts at: 2500 2500
Size: 6409
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4478 4510 5991 6023 4478
Size: 32 1481 32 4864
Fragments arranged by size:
4864 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3226 3510 3226
Size: 284 6125
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6409
PsiI TTA'TAA
Cuts at: 3979 3979
Size: 6409
PstI C_TGCA'G
Cuts at: 830 2046 2574 3761 830
Size: 1216 528 1187 3478
Fragments arranged by size:
3478 1216 1187 528
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6409
PvuII CAG'CTG
Cuts at: 3157 3157
Size: 6409
SacI G_AGCT'C
Cuts at: 2252 2252
Size: 6409
SalI G'TCGA_C
Cuts at: 1936 3751 1936
Size: 1815 4594
SapI GCTCTTCn'nnn_
Cuts at: 2257 2346 3050 2257
Size: 89 704 5616
Fragments arranged by size:
5616 704 89
SbfI CC_TGCA'GG
Cuts at: 3761 3761
Size: 6409
ScaI AGT'ACT
Cuts at: 4657 6369 4657
Size: 1712 4697
SexAI A'CCwGG_T
Cuts at: 2175 2175
Size: 6409
SfcI C'TryA_G
Cuts at: 826 1045 2042 2570 2698 3757 4892 5573 5764
Size: 219 997 528 128 1059 1135 681 191
Cuts at: 5764 826
Size: 1471
Fragments arranged by size:
1471 1135 1059 997 681 528 219 191
128
SfiI GGCCn_nnn'nGGCC
Cuts at: 2759 2759
Size: 6409
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6409
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6409
SmaI CCC'GGG
Cuts at: 1872 3736 1872
Size: 1864 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2476 4513 5414 5676 820
Size: 197 926 533 2037 901 262 1553
Fragments arranged by size:
2037 1553 926 901 533 262 197
SnaBI TAC'GTA
Cuts at: 493 4200 493
Size: 3707 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6409
SphI G_CATG'C
Cuts at: 1132 3767 1132
Size: 2635 3774
SspI AAT'ATT
Cuts at: 5 52 4333 5
Size: 47 4281 2081
Fragments arranged by size:
4281 2081 47
StuI AGG'CCT
Cuts at: 1517 3141 1517
Size: 1624 4785
StyI C'CwwG_G
Cuts at: 513 1065 2004 3464 3848 513
Size: 552 939 1460 384 3074
Fragments arranged by size:
3074 1460 939 552 384
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2216 3104 4436 4453 4606 4791 5236
Size: 1255 636 888 1332 17 153 185 445
Cuts at: 5236 325
Size: 1498
Fragments arranged by size:
1498 1332 1255 888 636 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2154 2267 4655
Size: 274 80 33 51 1443 177 113 2388
Cuts at: 4655 6367 96
Size: 1712 138
Fragments arranged by size:
2388 1712 1443 274 177 138 113 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2495 2527 2578 2610 6369 6401 2495
Size: 32 51 32 3759 32 2503
Fragments arranged by size:
3759 2503 51 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3316 1316
Size: 2000 4409
XbaI T'CTAG_A
Cuts at: 3631 3745 3631
Size: 114 6295
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6409
XmaI C'CCGG_G
Cuts at: 1870 3734 1870
Size: 1864 4545
XmnI GAAnn'nnTTC
Cuts at: 2439 2928 4538 2439
Size: 489 1610 4310
Fragments arranged by size:
4310 1610 489
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3521 4217 276
Size: 53 83 186 936 1987 696 2468
Fragments arranged by size:
2468 1987 936 696 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstXI BstZ17I EcoRV FseI FspAI
KasI NarI PacI PspOMI PspXI PsrI RsrII SacII
SanDI SfoI SrfI SwaI XcmI