Restriction map for
pFN21AB4944
(Circular) (Six-base) MAPSORT of: pFN21AB4944.seq Check: 3512 from: 1 to: 6241
pFN21AB4944 6241 bp
With 182 enzymes: SgfI *
October 20, 2009 10:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4051 278
Size: 53 83 186 936 2515 2468
Fragments arranged by size:
2515 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3584 1937
Size: 1647 4594
Acc65I G'GTAC_C
Cuts at: 3562 3562
Size: 6241
AclI AA'CG_TT
Cuts at: 1563 4368 4741 1563
Size: 2805 373 3063
Fragments arranged by size:
3063 2805 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3359 4304 5688 5956 1632
Size: 1727 945 1384 268 1917
Fragments arranged by size:
1917 1727 1384 945 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6044
AflIII A'CryG_T
Cuts at: 1203 5140 6117 1203
Size: 3937 977 1327
Fragments arranged by size:
3937 1327 977
AgeI A'CCGG_T
Cuts at: 5072 5072
Size: 6241
AhdI GACnn_n'nnGTC
Cuts at: 4970 4970
Size: 6241
AleI CACnn'nnGTG
Cuts at: 1166 3462 1166
Size: 2296 3945
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6241
AlwNI CAG_nnn'CTG
Cuts at: 3984 5556 3984
Size: 1572 4669
ApaLI G'TGCA_C
Cuts at: 4298 5454 4298
Size: 1156 5085
ApoI r'AATT_y
Cuts at: 1493 2792 3550 3776 3928 6037 1493
Size: 1299 758 226 152 2109 1697
Fragments arranged by size:
2109 1697 1299 758 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2406 3423 4795 160
Size: 872 1374 1017 1372 1606
Fragments arranged by size:
1606 1374 1372 1017 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6241
AvaI C'yCGr_G
Cuts at: 1870 1943 3348 3566 1870
Size: 73 1405 218 4545
Fragments arranged by size:
4545 1405 218 73
BamHI G'GATC_C
Cuts at: 3571 3938 3571
Size: 367 5874
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3562 5017 618
Size: 325 217 627 1775 1455 1842
Fragments arranged by size:
1842 1775 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2833 3560 1391
Size: 1442 727 4072
Fragments arranged by size:
4072 1442 727
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2225 2537 2726 928
Size: 971 326 312 189 4443
Fragments arranged by size:
4443 971 326 312 189
BbvCI CC'TCA_GC
Cuts at: 3487 3487
Size: 6241
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4432 4466 1903
Size: 34 2495 34 3678
Fragments arranged by size:
3678 2495 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4133 5343 4133
Size: 1210 5031
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6241
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4852 136
Size: 107 122 71 631 605 3180 1525
Fragments arranged by size:
3180 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6236 6236
Size: 6241
BlpI GC'TnA_GC
Cuts at: 3658 3658
Size: 6241
Bme1580I G_kGCm'C
Cuts at: 1790 4302 5458 1790
Size: 2512 1156 2573
Fragments arranged by size:
2573 2512 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6241
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3972 4930 442
Size: 416 53 83 399 24 2555 958 1753
Fragments arranged by size:
2555 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6241
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4901 1239
Size: 3662 2579
Bpu10I CC'TnA_GC
Cuts at: 3487 3487
Size: 6241
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3239 4366 5231 5529 3239
Size: 1127 865 298 3951
Fragments arranged by size:
3951 1127 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4904 882
Size: 617 3405 2219
Fragments arranged by size:
3405 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4032 493
Size: 711 2828 2702
Fragments arranged by size:
2828 2702 711
BsaBI GATnn'nnATC
Cuts at: 6235 6235
Size: 6241
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3291 4048 4430 5065
Size: 53 83 186 936 1758 757 382 635
Cuts at: 5065 275
Size: 1451
Fragments arranged by size:
1758 1451 936 757 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3387 4674 5072 5346 5493 5904 3387
Size: 1287 398 274 147 411 3724
Fragments arranged by size:
3724 1287 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2638 2668 1178
Size: 30 165 30 1235 30 4751
Fragments arranged by size:
4751 1235 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6241
BseYI C'CCAG_C
Cuts at: 2849 5444 5940 2849
Size: 2595 496 3150
Fragments arranged by size:
3150 2595 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5668
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2041 3691 4452 5480 1540
Size: 275 186 40 1650 761 1028 2301
Fragments arranged by size:
2301 1650 1028 761 275 186 40
BsiHKAI G_wGCw'C
Cuts at: 2733 2762 3099 4302 4387 5458 2733
Size: 29 337 1203 85 1071 3516
Fragments arranged by size:
3516 1203 1071 337 85 29
BsmI GAATG_Cn'
Cuts at: 2453 3438 3752 3845 2453
Size: 985 314 93 4849
Fragments arranged by size:
4849 985 314 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2733 2762 2833 3099 3560 4302 4387
Size: 399 943 29 71 266 461 742 85
Cuts at: 4387 5458 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 943 742 461 399 266 85
71 29
BspEI T'CCGG_A
Cuts at: 3387 5904 3387
Size: 2517 3724
BspHI T'CATG_A
Cuts at: 2443 2976 4130 5860 2443
Size: 533 1154 1730 2824
Fragments arranged by size:
2824 1730 1154 533
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2688 3596 844
Size: 1844 908 3489
Fragments arranged by size:
3489 1844 908
BsrBI CCG'CTC
Cuts at: 1394 1823 4128 1394
Size: 429 2305 3507
Fragments arranged by size:
3507 2305 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2719 4736 4910 65
Size: 1183 1471 2017 174 1396
Fragments arranged by size:
2017 1471 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4885 5072 1700
Size: 110 3075 187 2869
Fragments arranged by size:
3075 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2584 2862 96
Size: 2488 278 3475
Fragments arranged by size:
3475 2488 278
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6241
BssSI C'ACGA_G
Cuts at: 4301 5313 4301
Size: 1012 5229
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6241
BstBI TT'CG_AA
Cuts at: 5045 5045
Size: 6241
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6241
BstXI CCAn_nnnn'nTGG
Cuts at: 2849 2849
Size: 6241
BstYI r'GATC_y
Cuts at: 1972 3384 3571 3938 4323 4340 5792 5878 5890
Size: 1412 187 367 385 17 1452 86 12
Cuts at: 5890 6236 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1412 385 367 346 187 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2207 2207
Size: 6241
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5689
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5502
Fragments arranged by size:
5502 683 56
BtsI GCAGTG_nn'
Cuts at: 3757 4551 4571 3757
Size: 794 20 5427
Fragments arranged by size:
5427 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6241
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6206
DraI TTT'AAA
Cuts at: 2130 2400 3545 2130
Size: 270 1145 4826
Fragments arranged by size:
4826 1145 270
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5248 809
Size: 507 3932 1802
Fragments arranged by size:
3932 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3688 4577 6206
Size: 54 1425 50 275 18 1858 889 1629
Cuts at: 6206 8
Size: 43
Fragments arranged by size:
1858 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3688 1537
Size: 275 1876 4090
Fragments arranged by size:
4090 1876 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2157 2864 4171 1353
Size: 804 707 1307 3423
Fragments arranged by size:
3423 1307 804 707
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4828 5202 5348 387
Size: 1064 138 3239 374 146 1280
Fragments arranged by size:
3239 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3359 4304 4901 5688 5956 1239
Size: 393 1727 945 597 787 268 1524
Fragments arranged by size:
1727 1524 945 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3259 3259
Size: 6241
EcoRI G'AATT_C
Cuts at: 3550 3928 3550
Size: 378 5863
FspI TGC'GCA
Cuts at: 3507 4747 3507
Size: 1240 5001
HaeII r_GCGC'y
Cuts at: 1678 2020 5388 1678
Size: 342 3368 2531
Fragments arranged by size:
3368 2531 342
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2395 2427 4882 4914 4956
Size: 32 135 32 823 32 2455 32 42
Cuts at: 4956 4988 1373
Size: 32 2626
Fragments arranged by size:
2626 2455 823 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3585 3831 669
Size: 988 281 1647 246 3079
Fragments arranged by size:
3079 1647 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6241
HpaI GTT'AAC
Cuts at: 3831 3831
Size: 6241
KpnI G_GTAC'C
Cuts at: 3566 3566
Size: 6241
MfeI C'AATT_G
Cuts at: 3840 3840
Size: 6241
MluI A'CGCG_T
Cuts at: 6117 6117
Size: 6241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2093 2284 5355 5539 1305
Size: 788 191 3071 184 2007
Fragments arranged by size:
3071 2007 788 191 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4419
Fragments arranged by size:
4419 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3080 3462 3498 4199 4558 4717 518
Size: 648 1914 382 36 701 359 159 2042
Fragments arranged by size:
2042 1914 701 648 382 359 159 36
MspA1I CmG'CkG
Cuts at: 1606 3657 4334 5482 5727 1606
Size: 2051 677 1148 245 2120
Fragments arranged by size:
2120 2051 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5689
NdeI CA'TA_TG
Cuts at: 387 3104 387
Size: 2717 3524
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6241
NotI GC'GGCC_GC
Cuts at: 3688 3688
Size: 6241
NspI r_CATG'y
Cuts at: 1132 1229 3503 3599 5144 1132
Size: 97 2274 96 1545 2229
Fragments arranged by size:
2274 2229 1545 97 96
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6241
PciI A'CATG_T
Cuts at: 5140 5140
Size: 6241
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6241
PfoI T'CCnGG_A
Cuts at: 2009 2009
Size: 6241
PmeI GTTT'AAAC
Cuts at: 3545 3545
Size: 6241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4310 4342 5823 5855 4310
Size: 32 1481 32 4696
Fragments arranged by size:
4696 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6241
PsiI TTA'TAA
Cuts at: 3811 3811
Size: 6241
PstI C_TGCA'G
Cuts at: 830 2785 3593 830
Size: 1955 808 3478
Fragments arranged by size:
3478 1955 808
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6241
SalI G'TCGA_C
Cuts at: 1936 3583 1936
Size: 1647 4594
SapI GCTCTTCn'nnn_
Cuts at: 2864 2864
Size: 6241
SbfI CC_TGCA'GG
Cuts at: 3593 3593
Size: 6241
ScaI AGT'ACT
Cuts at: 4489 6201 4489
Size: 1712 4529
SfcI C'TryA_G
Cuts at: 826 1045 2770 2781 3153 3589 4724 5405 5596
Size: 219 1725 11 372 436 1135 681 191
Cuts at: 5596 826
Size: 1471
Fragments arranged by size:
1725 1471 1135 681 436 372 219 191
11
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6241
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6241
SmaI CCC'GGG
Cuts at: 1872 3350 3568 1872
Size: 1478 218 4545
Fragments arranged by size:
4545 1478 218
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3254 4345 5246 5508 820
Size: 197 926 1311 1091 901 262 1553
Fragments arranged by size:
1553 1311 1091 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4032 493
Size: 3539 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6241
SphI G_CATG'C
Cuts at: 1132 3599 1132
Size: 2467 3774
SspI AAT'ATT
Cuts at: 5 52 2501 4165 5
Size: 47 2449 1664 2081
Fragments arranged by size:
2449 2081 1664 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6241
StyI C'CwwG_G
Cuts at: 513 1065 2724 3520 3680 513
Size: 552 1659 796 160 3074
Fragments arranged by size:
3074 1659 796 552 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2330 3432 4268 4285 4438 4623 5068
Size: 1255 750 1102 836 17 153 185 445
Cuts at: 5068 325
Size: 1498
Fragments arranged by size:
1498 1255 1102 836 750 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2584 2862 4487
Size: 274 80 33 51 1443 607 278 1625
Cuts at: 4487 6199 96
Size: 1712 138
Fragments arranged by size:
1712 1625 1443 607 278 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2872 2904 6201 6233 2872
Size: 32 3297 32 2880
Fragments arranged by size:
3297 2880 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2042 1316
Size: 726 5515
XbaI T'CTAG_A
Cuts at: 3577 3577
Size: 6241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3012 3199 3012
Size: 187 6054
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6241
XmaI C'CCGG_G
Cuts at: 1870 3348 3566 1870
Size: 1478 218 4545
Fragments arranged by size:
4545 1478 218
XmnI GAAnn'nnTTC
Cuts at: 4370 4370
Size: 6241
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4049 276
Size: 53 83 186 936 2515 2468
Fragments arranged by size:
2515 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI