Restriction map for
pFN21AB4920
(Circular) (Six-base) MAPSORT of: pFN21AB4920.seq Check: 4449 from: 1 to: 6988
pFN21AB4920 6988 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4798 278
Size: 53 83 186 936 3262 2468
Fragments arranged by size:
3262 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2149 2535 4331 1937
Size: 212 386 1796 4594
Fragments arranged by size:
4594 1796 386 212
Acc65I G'GTAC_C
Cuts at: 4309 4309
Size: 6988
AclI AA'CG_TT
Cuts at: 1563 5115 5488 1563
Size: 3552 373 3063
Fragments arranged by size:
3552 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3286 3370 3454 3560 3790 5051 6435 6703
Size: 1654 84 84 106 230 1261 1384 268
Cuts at: 6703 1632
Size: 1917
Fragments arranged by size:
1917 1654 1384 1261 268 230 106 84
84
AflII C'TTAA_G
Cuts at: 820 1017 2228 820
Size: 197 1211 5580
Fragments arranged by size:
5580 1211 197
AflIII A'CryG_T
Cuts at: 1203 4258 5887 6864 1203
Size: 3055 1629 977 1327
Fragments arranged by size:
3055 1629 1327 977
AgeI A'CCGG_T
Cuts at: 5819 5819
Size: 6988
AhdI GACnn_n'nnGTC
Cuts at: 3555 5717 3555
Size: 2162 4826
AleI CACnn'nnGTG
Cuts at: 1166 3288 1166
Size: 2122 4866
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6988
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2017 2049 2017
Size: 32 6956
AlwNI CAG_nnn'CTG
Cuts at: 4731 6303 4731
Size: 1572 5416
ApaLI G'TGCA_C
Cuts at: 3312 3396 3902 5045 6201 3312
Size: 84 506 1143 1156 4099
Fragments arranged by size:
4099 1156 1143 506 84
ApoI r'AATT_y
Cuts at: 1493 2155 2164 2265 2697 2862 3131 3336 3672
Size: 662 9 101 432 165 269 205 336
Cuts at: 3672 4176 4297 4523 4675 6784 1493
Size: 504 121 226 152 2109 1697
Fragments arranged by size:
2109 1697 662 504 432 336 269 226
205 165 152 121 101 9
AseI AT'TA_AT
Cuts at: 160 1032 3492 5542 160
Size: 872 2460 2050 1606
Fragments arranged by size:
2460 2050 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6988
AvaI C'yCGr_G
Cuts at: 1870 1943 4313 1870
Size: 73 2370 4545
Fragments arranged by size:
4545 2370 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3151 3184 3151
Size: 33 6955
BamHI G'GATC_C
Cuts at: 4318 4685 4318
Size: 367 6621
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4271 4309 5764 618
Size: 325 217 627 2484 38 1455 1842
Fragments arranged by size:
2484 1842 1455 627 325 217 38
BanII G_rGCy'C
Cuts at: 1391 2223 4307 1391
Size: 832 2084 4072
Fragments arranged by size:
4072 2084 832
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2454 2811 3554 928
Size: 971 555 357 743 4362
Fragments arranged by size:
4362 971 743 555 357
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2205 2239 5179 5213 1903
Size: 34 268 34 2940 34 3678
Fragments arranged by size:
3678 2940 268 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2352 4880 6090 2352
Size: 2528 1210 3250
Fragments arranged by size:
3250 2528 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6988
BfrBI ATG'CAT
Cuts at: 4202 4202
Size: 6988
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5599 136
Size: 107 122 71 631 605 3927 1525
Fragments arranged by size:
3927 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6983 6983
Size: 6988
BlpI GC'TnA_GC
Cuts at: 4405 4405
Size: 6988
Bme1580I G_kGCm'C
Cuts at: 1790 3316 3400 3906 5049 6205 1790
Size: 1526 84 506 1143 1156 2573
Fragments arranged by size:
2573 1526 1156 1143 506 84
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6988
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2332 2563 3606
Size: 416 53 83 399 24 915 231 1043
Cuts at: 3606 4719 5677 442
Size: 1113 958 1753
Fragments arranged by size:
1753 1113 1043 958 915 416 399 231
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6988
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2269 2269
Size: 6988
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2306 2733 3114 3282 3366 3450 3534 3702
Size: 1067 427 381 168 84 84 84 168
Cuts at: 3702 3786 3870 4038 4122 5648 1239
Size: 84 84 168 84 1526 2579
Fragments arranged by size:
2579 1526 1067 427 381 168 168 168
84 84 84 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1999 5113 5978 6276 1999
Size: 3114 865 298 2711
Fragments arranged by size:
3114 2711 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2135 2233 4102 5651 882
Size: 617 636 98 1869 1549 2219
Fragments arranged by size:
2219 1869 1549 636 617 98
BsaAI yAC'GTr
Cuts at: 493 1204 4779 493
Size: 711 3575 2702
Fragments arranged by size:
3575 2702 711
BsaBI GATnn'nnATC
Cuts at: 6982 6982
Size: 6988
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4795 5177 5812 275
Size: 53 83 186 936 3262 382 635 1451
Fragments arranged by size:
3262 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5421 5819 6093 6240 6651 5421
Size: 398 274 147 411 5758
Fragments arranged by size:
5758 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2747 2777 1178
Size: 30 165 30 1344 30 5389
Fragments arranged by size:
5389 1344 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2400 1186
Size: 1214 5774
BseYI C'CCAG_C
Cuts at: 6191 6687 6191
Size: 496 6492
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2594 1163
Size: 573 858 5557
Fragments arranged by size:
5557 858 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4438 5199 6227 1540
Size: 275 186 2437 761 1028 2301
Fragments arranged by size:
2437 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2223 3316 3400 3906 5049 5134 6205 2223
Size: 1093 84 506 1143 85 1071 3006
Fragments arranged by size:
3006 1143 1093 1071 506 85 84
BsmI GAATG_Cn'
Cuts at: 2974 3258 3284 3887 4499 4592 2974
Size: 284 26 603 612 93 5370
Fragments arranged by size:
5370 612 603 284 93 26
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2223 3316 3400 3906 4307 5049 5134
Size: 399 433 1093 84 506 401 742 85
Cuts at: 5134 6205 1391
Size: 1071 2174
Fragments arranged by size:
2174 1093 1071 742 506 433 401 399
85 84
BspEI T'CCGG_A
Cuts at: 6651 6651
Size: 6988
BspHI T'CATG_A
Cuts at: 4877 6607 4877
Size: 1730 5258
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4343 844
Size: 3499 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4875 1394
Size: 429 3052 3507
Fragments arranged by size:
3507 3052 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3541 3625 5483 5657 65
Size: 1183 2293 84 1858 174 1396
Fragments arranged by size:
2293 1858 1396 1183 174 84
BsrFI r'CCGG_y
Cuts at: 1700 1810 5632 5819 1700
Size: 110 3822 187 2869
Fragments arranged by size:
3822 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2643 2989 3073 96
Size: 2547 346 84 4011
Fragments arranged by size:
4011 2547 346 84
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6988
BssSI C'ACGA_G
Cuts at: 5048 6060 5048
Size: 1012 5976
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6988
BstBI TT'CG_AA
Cuts at: 5792 5792
Size: 6988
BstEII G'GTnAC_C
Cuts at: 1184 2497 1184
Size: 1313 5675
BstXI CCAn_nnnn'nTGG
Cuts at: 3290 3290
Size: 6988
BstYI r'GATC_y
Cuts at: 1972 4318 4685 5070 5087 6539 6625 6637 6983
Size: 2346 367 385 17 1452 86 12 346
Cuts at: 6983 1972
Size: 1977
Fragments arranged by size:
2346 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2678 513
Size: 552 1613 4823
Fragments arranged by size:
4823 1613 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6249
Fragments arranged by size:
6249 683 56
BtsI GCAGTG_nn'
Cuts at: 2370 3877 4504 5298 5318 2370
Size: 1507 627 794 20 4040
Fragments arranged by size:
4040 1507 794 627 20
ClaI AT'CG_AT
Cuts at: 1554 2510 1554
Size: 956 6032
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3523 3558 3943 3978 529
Size: 35 2959 35 385 35 3539
Fragments arranged by size:
3539 2959 385 35 35 35
DraI TTT'AAA
Cuts at: 3949 4292 3949
Size: 343 6645
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5995 809
Size: 507 4679 1802
Fragments arranged by size:
4679 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4435 5324 6953
Size: 54 1425 50 275 18 2605 889 1629
Cuts at: 6953 8
Size: 43
Fragments arranged by size:
2605 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4435 1537
Size: 275 2623 4090
Fragments arranged by size:
4090 2623 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2394 4918 1353
Size: 1041 2524 3423
Fragments arranged by size:
3423 2524 1041
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5575 5949 6095 387
Size: 1064 138 3986 374 146 1280
Fragments arranged by size:
3986 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2221 2221
Size: 6988
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2306 2733 3114 3282 3286 3366 3370
Size: 393 674 427 381 168 4 80 4
Cuts at: 3370 3450 3454 3534 3560 3702 3786 3790 3870
Size: 80 4 80 26 142 84 4 80
Cuts at: 3870 4038 4122 5051 5648 6435 6703 1239
Size: 168 84 929 597 787 268 1524
Fragments arranged by size:
1524 929 787 674 597 427 393 381
268 168 168 142 84 84 80 80
80 80 26 4 4 4 4
EcoO109I rG'GnC_Cy
Cuts at: 2708 2708
Size: 6988
EcoRI G'AATT_C
Cuts at: 3336 3672 4176 4297 4675 3336
Size: 336 504 121 378 5649
Fragments arranged by size:
5649 504 378 336 121
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2951 2951
Size: 6988
FspI TGC'GCA
Cuts at: 5494 5494
Size: 6988
HaeII r_GCGC'y
Cuts at: 1678 6135 1678
Size: 4457 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2747 2779 3481 3513 3541
Size: 32 135 32 1175 32 702 32 28
Cuts at: 3541 3573 3805 3837 5629 5661 5703 5735 1373
Size: 32 232 32 1792 32 42 32 2626
Fragments arranged by size:
2626 1792 1175 702 232 135 42 32
32 32 32 32 32 32 32 28
HincII GTy'rAC
Cuts at: 669 1657 1938 2150 2210 4332 4578 669
Size: 988 281 212 60 2122 246 3079
Fragments arranged by size:
3079 2122 988 281 246 212 60
HindIII A'AGCT_T
Cuts at: 748 4053 748
Size: 3305 3683
HpaI GTT'AAC
Cuts at: 4578 4578
Size: 6988
KpnI G_GTAC'C
Cuts at: 4313 4313
Size: 6988
MfeI C'AATT_G
Cuts at: 4587 4587
Size: 6988
MluI A'CGCG_T
Cuts at: 6864 6864
Size: 6988
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3608 6102 6286 1305
Size: 2303 2494 184 2007
Fragments arranged by size:
2494 2303 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5166
Fragments arranged by size:
5166 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2871 3277 3288 3445 3613 3865 4257
Size: 648 1705 406 11 157 168 252 392
Cuts at: 4257 4269 4284 4946 5305 5464 518
Size: 12 15 662 359 159 2042
Fragments arranged by size:
2042 1705 662 648 406 392 359 252
168 159 157 15 12 11
MspA1I CmG'CkG
Cuts at: 1606 4404 5081 6229 6474 1606
Size: 2798 677 1148 245 2120
Fragments arranged by size:
2798 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2678 513
Size: 552 1613 4823
Fragments arranged by size:
4823 1613 552
NdeI CA'TA_TG
Cuts at: 387 2812 3064 3274 387
Size: 2425 252 210 4101
Fragments arranged by size:
4101 2425 252 210
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6988
NotI GC'GGCC_GC
Cuts at: 4435 4435
Size: 6988
NsiI A_TGCA'T
Cuts at: 4204 4204
Size: 6988
NspI r_CATG'y
Cuts at: 1132 1229 2468 2745 4158 4206 4346 5891 1132
Size: 97 1239 277 1413 48 140 1545 2229
Fragments arranged by size:
2229 1545 1413 1239 277 140 97 48
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6988
PciI A'CATG_T
Cuts at: 5887 5887
Size: 6988
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6988
PfoI T'CCnGG_A
Cuts at: 3091 3091
Size: 6988
PmeI GTTT'AAAC
Cuts at: 4292 4292
Size: 6988
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5057 5089 6570 6602 5057
Size: 32 1481 32 5443
Fragments arranged by size:
5443 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3387 1613
Size: 1774 5214
PsiI TTA'TAA
Cuts at: 3696 4558 3696
Size: 862 6126
PstI C_TGCA'G
Cuts at: 830 4340 830
Size: 3510 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6988
SacI G_AGCT'C
Cuts at: 2223 2223
Size: 6988
SalI G'TCGA_C
Cuts at: 1936 2148 4330 1936
Size: 212 2182 4594
Fragments arranged by size:
4594 2182 212
SbfI CC_TGCA'GG
Cuts at: 4340 4340
Size: 6988
ScaI AGT'ACT
Cuts at: 3649 5236 6948 3649
Size: 1587 1712 3689
Fragments arranged by size:
3689 1712 1587
SfcI C'TryA_G
Cuts at: 826 1045 2532 2922 4336 5471 6152 6343 826
Size: 219 1487 390 1414 1135 681 191 1471
Fragments arranged by size:
1487 1471 1414 1135 681 390 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6988
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6988
SmaI CCC'GGG
Cuts at: 1872 4315 1872
Size: 2443 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2014 2228 5092 5993 6255 820
Size: 197 926 71 214 2864 901 262 1553
Fragments arranged by size:
2864 1553 926 901 262 214 197 71
SnaBI TAC'GTA
Cuts at: 493 4779 493
Size: 4286 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6988
SphI G_CATG'C
Cuts at: 1132 4346 1132
Size: 3214 3774
SspI AAT'ATT
Cuts at: 5 52 4912 5
Size: 47 4860 2081
Fragments arranged by size:
4860 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6988
StyI C'CwwG_G
Cuts at: 513 1065 2678 4427 513
Size: 552 1613 1749 3074
Fragments arranged by size:
3074 1749 1613 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2558 4244 5015 5032 5185 5370 5815
Size: 1255 978 1686 771 17 153 185 445
Cuts at: 5815 325
Size: 1498
Fragments arranged by size:
1686 1498 1255 978 771 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2643 2744 2989
Size: 274 80 33 51 1443 666 101 245
Cuts at: 2989 3073 3563 3647 4235 5234 6946 96
Size: 84 490 84 588 999 1712 138
Fragments arranged by size:
1712 1443 999 666 588 490 274 245
138 101 84 84 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2625 2657 6948 6980 2625
Size: 32 4291 32 2633
Fragments arranged by size:
4291 2633 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6988
XbaI T'CTAG_A
Cuts at: 2395 4324 2395
Size: 1929 5059
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6988
XmaI C'CCGG_G
Cuts at: 1870 4313 1870
Size: 2443 4545
XmnI GAAnn'nnTTC
Cuts at: 3383 3551 3676 3803 3887 4055 5117 3383
Size: 168 125 127 84 168 1062 5254
Fragments arranged by size:
5254 1062 168 168 127 125 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4796 276
Size: 53 83 186 936 3262 2468
Fragments arranged by size:
3262 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI