Restriction map for
pFN21AB4919
(Circular) (Six-base) MAPSORT of: pFN21AB4919.seq Check: 8922 from: 1 to: 10204
pFN21AB4919 10204 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2758 3504 3568 2758
Size: 746 64 9394
Fragments arranged by size:
9394 746 64
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8014 278
Size: 53 83 186 936 6478 2468
Fragments arranged by size:
6478 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7285 7547 1937
Size: 5348 262 4594
Fragments arranged by size:
5348 4594 262
Acc65I G'GTAC_C
Cuts at: 7525 7525
Size: 10204
AclI AA'CG_TT
Cuts at: 1563 8331 8704 1563
Size: 6768 373 3063
Fragments arranged by size:
6768 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2054 2484 2649 3406 3539 4845 5057 6024
Size: 422 430 165 757 133 1306 212 967
Cuts at: 6024 6237 6700 6781 8267 9651 9919 1632
Size: 213 463 81 1486 1384 268 1917
Fragments arranged by size:
1917 1486 1384 1306 967 757 463 430
422 268 213 212 165 133 81
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10007
AflIII A'CryG_T
Cuts at: 1203 7261 7501 9103 10080 1203
Size: 6058 240 1602 977 1327
Fragments arranged by size:
6058 1602 1327 977 240
AgeI A'CCGG_T
Cuts at: 9035 9035
Size: 10204
AhdI GACnn_n'nnGTC
Cuts at: 2521 8933 2521
Size: 6412 3792
AleI CACnn'nnGTG
Cuts at: 1166 2324 1166
Size: 1158 9046
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3516 4502 4808 1184
Size: 2332 986 306 6580
Fragments arranged by size:
6580 2332 986 306
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4149 4150 4181 4182 4149
Size: 1 31 1 10171
Fragments arranged by size:
10171 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 4348 5774 7947 9519 4348
Size: 1426 2173 1572 5033
Fragments arranged by size:
5033 2173 1572 1426
ApaLI G'TGCA_C
Cuts at: 7257 8261 9417 7257
Size: 1004 1156 8044
Fragments arranged by size:
8044 1156 1004
ApoI r'AATT_y
Cuts at: 1493 2915 5274 5788 7513 7739 7891 10000 1493
Size: 1422 2359 514 1725 226 152 2109 1697
Fragments arranged by size:
2359 2109 1725 1697 1422 514 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8758 160
Size: 872 7726 1606
Fragments arranged by size:
7726 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10204
AvaI C'yCGr_G
Cuts at: 1870 1943 3607 4806 7202 7529 1870
Size: 73 1664 1199 2396 327 4545
Fragments arranged by size:
4545 2396 1664 1199 327 73
AvrII C'CTAG_G
Cuts at: 2530 6398 2530
Size: 3868 6336
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4173 4206 5605 5638 4173
Size: 33 1399 33 8739
Fragments arranged by size:
8739 1399 33 33
BamHI G'GATC_C
Cuts at: 2191 4176 5462 6864 7534 7901 2191
Size: 1985 1286 1402 670 367 4494
Fragments arranged by size:
4494 1985 1402 1286 670 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2200 3522 4810 6017 6850
Size: 325 217 627 413 1322 1288 1207 833
Cuts at: 6850 7525 8980 618
Size: 675 1455 1842
Fragments arranged by size:
1842 1455 1322 1288 1207 833 675 627
413 325 217
BanII G_rGCy'C
Cuts at: 1391 2719 3608 4515 4910 6838 7236 7523 1391
Size: 1328 889 907 395 1928 398 287 4072
Fragments arranged by size:
4072 1928 1328 907 889 398 395 287
BbeI G_GCGC'C
Cuts at: 6021 6021
Size: 10204
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4752 6787 7107 928
Size: 971 2853 2035 320 4025
Fragments arranged by size:
4025 2853 2035 971 320
BbvCI CC'TCA_GC
Cuts at: 5865 5979 7312 5865
Size: 114 1333 8757
Fragments arranged by size:
8757 1333 114
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8395 8429 1903
Size: 34 6458 34 3678
Fragments arranged by size:
6458 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3804 8096 9306 3804
Size: 4292 1210 4702
Fragments arranged by size:
4702 4292 1210
BclI T'GATC_A
Cuts at: 1548 5845 6069 1548
Size: 4297 224 5683
Fragments arranged by size:
5683 4297 224
BfrBI ATG'CAT
Cuts at: 5296 5296
Size: 10204
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8815 136
Size: 107 122 71 631 605 7143 1525
Fragments arranged by size:
7143 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6406 10199 6406
Size: 3793 6411
BlpI GC'TnA_GC
Cuts at: 3602 3825 3870 7621 3602
Size: 223 45 3751 6185
Fragments arranged by size:
6185 3751 223 45
Bme1580I G_kGCm'C
Cuts at: 1790 4813 6855 7261 8265 9421 1790
Size: 3023 2042 406 1004 1156 2573
Fragments arranged by size:
3023 2573 2042 1156 1004 406
BmgBI CAC'GTC
Cuts at: 1318 5546 1318
Size: 4228 5976
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2693 5076 6117
Size: 416 53 83 399 24 1276 2383 1041
Cuts at: 6117 7935 8893 442
Size: 1818 958 1753
Fragments arranged by size:
2383 1818 1753 1276 1041 958 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10204
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3031 6280 6994 3031
Size: 3249 714 6241
Fragments arranged by size:
6241 3249 714
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3079 3509 4479 6291 6745 8864 1239
Size: 1840 430 970 1812 454 2119 2579
Fragments arranged by size:
2579 2119 1840 1812 970 454 430
Bpu10I CC'TnA_GC
Cuts at: 3150 5865 5979 7290 7312 3150
Size: 2715 114 1311 22 6042
Fragments arranged by size:
6042 2715 1311 114 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2176 4719 6274 8329 9194 9492 2176
Size: 2543 1555 2055 865 298 2888
Fragments arranged by size:
2888 2543 2055 1555 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2933 6540 6723 8867 882
Size: 617 1434 3607 183 2144 2219
Fragments arranged by size:
3607 2219 2144 1434 617 183
BsaAI yAC'GTr
Cuts at: 493 1204 4189 7995 493
Size: 711 2985 3806 2702
Fragments arranged by size:
3806 2985 2702 711
BsaBI GATnn'nnATC
Cuts at: 10198 10198
Size: 10204
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6018 8011 8393 9028
Size: 53 83 186 936 4485 1993 382 635
Cuts at: 9028 275
Size: 1451
Fragments arranged by size:
4485 1993 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3510 4799 7173 8637 9035 9309 9456 9867 3510
Size: 1289 2374 1464 398 274 147 411 3847
Fragments arranged by size:
3847 2374 1464 1289 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 9979
Fragments arranged by size:
9979 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2504 2864 3004 3540 4592 4702 4717 5986
Size: 1318 360 140 536 1052 110 15 1269
Cuts at: 5986 6251 6256 6962 7054 7059 1186
Size: 265 5 706 92 5 4331
Fragments arranged by size:
4331 1318 1269 1052 706 536 360 265
140 110 92 15 5 5
BseYI C'CCAG_C
Cuts at: 2949 3065 3283 3627 4133 4348 4644 4781 5009
Size: 116 218 344 506 215 296 137 228
Cuts at: 5009 9407 9903 2949
Size: 4398 496 3250
Fragments arranged by size:
4398 3250 506 496 344 296 228 218
215 137 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2754 2792 3126 3602 7247 1163
Size: 573 1018 38 334 476 3645 4120
Fragments arranged by size:
4120 3645 1018 573 476 334 38
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7654 8415 9443 1540
Size: 275 186 5653 761 1028 2301
Fragments arranged by size:
5653 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3012 3495 3608 3954 4515 7094 7261 8265 8350
Size: 483 113 346 561 2579 167 1004 85
Cuts at: 8350 9421 3012
Size: 1071 3795
Fragments arranged by size:
3795 2579 1071 1004 561 483 346 167
113 85
BsmI GAATG_Cn'
Cuts at: 3172 3778 4491 7715 7808 3172
Size: 606 713 3224 93 5568
Fragments arranged by size:
5568 3224 713 606 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4877 4877
Size: 10204
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2719 3012 3495 3608 3954 4515 4813
Size: 399 929 293 483 113 346 561 298
Cuts at: 4813 4910 6838 6855 7094 7236 7261 7523 8265
Size: 97 1928 17 239 142 25 262 742
Cuts at: 8265 8350 9421 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1928 1071 929 742 561 483 399
346 298 293 262 239 142 113 97
85 25 17
BspEI T'CCGG_A
Cuts at: 7173 9867 7173
Size: 2694 7510
BspHI T'CATG_A
Cuts at: 8093 9823 8093
Size: 1730 8474
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2758 3504 3568 3976 4090 7559 844
Size: 1914 746 64 408 114 3469 3489
Fragments arranged by size:
3489 3469 1914 746 408 114 64
BsrBI CCG'CTC
Cuts at: 1394 1823 7123 8091 1394
Size: 429 5300 968 3507
Fragments arranged by size:
5300 3507 968 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2204 4335 6920 7161 8699 8873 65
Size: 1183 956 2131 2585 241 1538 174 1396
Fragments arranged by size:
2585 2131 1538 1396 1183 956 241 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5309 8848 9035 1700
Size: 110 3499 3539 187 2869
Fragments arranged by size:
3539 3499 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4212 7151 96
Size: 4116 2939 3149
Fragments arranged by size:
4116 3149 2939
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10204
BssSI C'ACGA_G
Cuts at: 8264 9276 8264
Size: 1012 9192
BstAPI GCAn_nnn'nTGC
Cuts at: 794 6829 794
Size: 6035 4169
BstBI TT'CG_AA
Cuts at: 9008 9008
Size: 10204
BstEII G'GTnAC_C
Cuts at: 1184 4300 1184
Size: 3116 7088
BstXI CCAn_nnnn'nTGG
Cuts at: 4507 4507
Size: 10204
BstYI r'GATC_y
Cuts at: 1972 2191 4176 5462 6406 6864 7474 7534 7901
Size: 219 1985 1286 944 458 610 60 367
Cuts at: 7901 8286 8303 9755 9841 9853 10199 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1985 1977 1452 1286 944 610 458 385
367 346 219 86 60 17 12
Bsu36I CC'TnA_GG
Cuts at: 3016 6702 6774 3016
Size: 3686 72 6446
Fragments arranged by size:
6446 3686 72
BtgI C'CryG_G
Cuts at: 513 1065 2196 2394 2958 3366 3526 6028 6636
Size: 552 1131 198 564 408 160 2502 608
Cuts at: 6636 6846 7182 513
Size: 210 336 3535
Fragments arranged by size:
3535 2502 1131 608 564 552 408 336
210 198 160
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9465
Fragments arranged by size:
9465 683 56
BtsI GCAGTG_nn'
Cuts at: 3614 5265 7720 8514 8534 3614
Size: 1651 2455 794 20 5284
Fragments arranged by size:
5284 2455 1651 794 20
ClaI AT'CG_AT
Cuts at: 1554 4878 1554
Size: 3324 6880
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10169
DraI TTT'AAA
Cuts at: 2166 7508 2166
Size: 5342 4862
DraIII CAC_nnn'GTG
Cuts at: 6125 6125
Size: 10204
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2081 9211 809
Size: 507 765 7130 1802
Fragments arranged by size:
7130 1802 765 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4461 5548 7651
Size: 54 1425 50 275 18 2631 1087 2103
Cuts at: 7651 8540 10169 8
Size: 889 1629 43
Fragments arranged by size:
2631 2103 1629 1425 1087 889 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7651 1537
Size: 275 5839 4090
Fragments arranged by size:
5839 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2261 2277 3558 3878 4520 4944 5040 5247
Size: 908 16 1281 320 642 424 96 207
Cuts at: 5247 6451 6538 6556 6685 7182 8134 1353
Size: 1204 87 18 129 497 952 3423
Fragments arranged by size:
3423 1281 1204 952 908 642 497 424
320 207 129 96 87 18 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8791 9165 9311 387
Size: 1064 138 7202 374 146 1280
Fragments arranged by size:
7202 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3606 4513 3606
Size: 907 9297
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2484 2649 3079 3406 3509 3539
Size: 393 422 430 165 430 327 103 30
Cuts at: 3539 4479 4845 5057 6024 6237 6291 6700 6745
Size: 940 366 212 967 213 54 409 45
Cuts at: 6745 6781 8267 8864 9651 9919 1239
Size: 36 1486 597 787 268 1524
Fragments arranged by size:
1524 1486 967 940 787 597 430 430
422 409 393 366 327 268 213 212
165 103 54 45 36 30
EcoNI CCTnn'n_nnAGG
Cuts at: 6271 7293 6271
Size: 1022 9182
EcoO109I rG'GnC_Cy
Cuts at: 3565 3587 4621 6674 6779 6985 7022 3565
Size: 22 1034 2053 105 206 37 6747
Fragments arranged by size:
6747 2053 1034 206 105 37 22
EcoRI G'AATT_C
Cuts at: 7513 7891 7513
Size: 378 9826
EcoRV GAT'ATC
Cuts at: 4876 6532 4876
Size: 1656 8548
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3415 3415
Size: 10204
FspI TGC'GCA
Cuts at: 8710 8710
Size: 10204
HaeII r_GCGC'y
Cuts at: 1678 6021 6596 9351 1678
Size: 4343 575 2755 2531
Fragments arranged by size:
4343 2755 2531 575
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2507 2539 3259 3291 4106
Size: 32 135 32 935 32 720 32 815
Cuts at: 4106 4138 4874 4906 5447 5479 6825 6857 7466
Size: 32 736 32 541 32 1346 32 609
Cuts at: 7466 7498 8845 8877 8919 8951 1373
Size: 32 1347 32 42 32 2626
Fragments arranged by size:
2626 1347 1346 935 815 736 720 609
541 135 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4080 7548 7794 669
Size: 988 281 2142 3468 246 3079
Fragments arranged by size:
3468 3079 2142 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3134 3414 5315 748
Size: 2386 280 1901 5637
Fragments arranged by size:
5637 2386 1901 280
HpaI GTT'AAC
Cuts at: 7794 7794
Size: 10204
KasI G'GCGC_C
Cuts at: 6017 6017
Size: 10204
KpnI G_GTAC'C
Cuts at: 7529 7529
Size: 10204
MfeI C'AATT_G
Cuts at: 6663 7803 6663
Size: 1140 9064
MluI A'CGCG_T
Cuts at: 10080 10080
Size: 10204
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2069 3706 7404 9318 9502 1305
Size: 764 1637 3698 1914 184 2007
Fragments arranged by size:
3698 2007 1914 1637 764 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4463 10
Size: 54 1425 343 2631 5751
Fragments arranged by size:
5751 2631 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2294 2324 3498 3644 4901 5048 5631
Size: 648 1128 30 1174 146 1257 147 583
Cuts at: 5631 7338 8162 8521 8680 518
Size: 1707 824 359 159 2042
Fragments arranged by size:
2042 1707 1257 1174 1128 824 648 583
359 159 147 146 30
MspA1I CmG'CkG
Cuts at: 1606 2960 3287 3487 3601 4648 4945 5013 7620
Size: 1354 327 200 114 1047 297 68 2607
Cuts at: 7620 8297 9445 9690 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2607 2120 1354 1148 1047 677 327 297
245 200 114 68
NarI GG'CG_CC
Cuts at: 6018 6018
Size: 10204
NcoI C'CATG_G
Cuts at: 513 1065 2394 3526 6636 6846 513
Size: 552 1329 1132 3110 210 3871
Fragments arranged by size:
3871 3110 1329 1132 552 210
NdeI CA'TA_TG
Cuts at: 387 4262 5700 387
Size: 3875 1438 4891
Fragments arranged by size:
4891 3875 1438
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10204
NotI GC'GGCC_GC
Cuts at: 7651 7651
Size: 10204
NsiI A_TGCA'T
Cuts at: 5298 5298
Size: 10204
NspI r_CATG'y
Cuts at: 1132 1229 2227 3503 3944 4361 4987 5605 5830
Size: 97 998 1276 441 417 626 618 225
Cuts at: 5830 6247 7265 7505 7562 9107 1132
Size: 417 1018 240 57 1545 2229
Fragments arranged by size:
2229 1545 1276 1018 998 626 618 441
417 417 240 225 97 57
PasI CC'CwG_GG
Cuts at: 1348 3445 3569 4468 6052 6273 7249 1348
Size: 2097 124 899 1584 221 976 4303
Fragments arranged by size:
4303 2097 1584 976 899 221 124
PciI A'CATG_T
Cuts at: 7261 7501 9103 7261
Size: 240 1602 8362
Fragments arranged by size:
8362 1602 240
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4199 4348 4682 1108
Size: 3091 149 334 6630
Fragments arranged by size:
6630 3091 334 149
PfoI T'CCnGG_A
Cuts at: 2548 6307 2548
Size: 3759 6445
PmeI GTTT'AAAC
Cuts at: 7508 7508
Size: 10204
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8273 8305 9786 9818 8273
Size: 32 1481 32 8659
Fragments arranged by size:
8659 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3565 3587 4621 6779 6985 7022 3565
Size: 22 1034 2158 206 37 6747
Fragments arranged by size:
6747 2158 1034 206 37 22
PshAI GACnn'nnGTC
Cuts at: 1613 2445 4298 1613
Size: 832 1853 7519
Fragments arranged by size:
7519 1853 832
PsiI TTA'TAA
Cuts at: 7774 7774
Size: 10204
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2008 2040 2008
Size: 32 10172
PstI C_TGCA'G
Cuts at: 830 2306 2364 2463 5272 7556 830
Size: 1476 58 99 2809 2284 3478
Fragments arranged by size:
3478 2809 2284 1476 99 58
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10204
PvuII CAG'CTG
Cuts at: 3487 4648 4945 3487
Size: 1161 297 8746
Fragments arranged by size:
8746 1161 297
RsrII CG'GwC_CG
Cuts at: 3513 3513
Size: 10204
SacI G_AGCT'C
Cuts at: 3608 4515 3608
Size: 907 9297
SacII CC_GC'GG
Cuts at: 2961 2961
Size: 10204
SalI G'TCGA_C
Cuts at: 1936 7546 1936
Size: 5610 4594
SapI GCTCTTCn'nnn_
Cuts at: 2261 3878 4520 5247 2261
Size: 1617 642 727 7218
Fragments arranged by size:
7218 1617 727 642
SbfI CC_TGCA'GG
Cuts at: 7556 7556
Size: 10204
ScaI AGT'ACT
Cuts at: 8452 10164 8452
Size: 1712 8492
SfcI C'TryA_G
Cuts at: 826 1045 2137 2302 2360 2459 3798 5268 7552
Size: 219 1092 165 58 99 1339 1470 2284
Cuts at: 7552 8687 9368 9559 826
Size: 1135 681 191 1471
Fragments arranged by size:
2284 1471 1470 1339 1135 1092 681 219
191 165 99 58
SfoI GGC'GCC
Cuts at: 6019 6019
Size: 10204
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10204
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10204
SmaI CCC'GGG
Cuts at: 1872 4808 7531 1872
Size: 2936 2723 4545
Fragments arranged by size:
4545 2936 2723
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2155 4734 6289 8308 9209 9471
Size: 197 926 212 2579 1555 2019 901 262
Cuts at: 9471 820
Size: 1553
Fragments arranged by size:
2579 2019 1555 1553 926 901 262 212
197
SnaBI TAC'GTA
Cuts at: 493 7995 493
Size: 7502 2702
SpeI A'CTAG_T
Cuts at: 152 5401 152
Size: 5249 4955
SphI G_CATG'C
Cuts at: 1132 3503 4361 4987 5605 6247 7562 1132
Size: 2371 858 626 618 642 1315 3774
Fragments arranged by size:
3774 2371 1315 858 642 626 618
SspI AAT'ATT
Cuts at: 5 52 2183 5496 8128 5
Size: 47 2131 3313 2632 2081
Fragments arranged by size:
3313 2632 2131 2081 47
StuI AGG'CCT
Cuts at: 1517 3051 5302 1517
Size: 1534 2251 6419
Fragments arranged by size:
6419 2251 1534
StyI C'CwwG_G
Cuts at: 513 1065 2394 2530 3526 6398 6503 6636 6846
Size: 552 1329 136 996 2872 105 133 210
Cuts at: 6846 6981 7643 513
Size: 135 662 3074
Fragments arranged by size:
3074 2872 1329 996 662 552 210 136
135 133 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2861 2989 3297 4047 6136 6430 6931
Size: 1255 1281 128 308 750 2089 294 501
Cuts at: 6931 7334 8231 8248 8401 8586 9031 325
Size: 403 897 17 153 185 445 1498
Fragments arranged by size:
2089 1498 1281 1255 897 750 501 445
403 308 294 185 153 128 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4212 5559 7151
Size: 274 80 33 51 1443 2235 1347 1592
Cuts at: 7151 8450 10162 96
Size: 1299 1712 138
Fragments arranged by size:
2235 1712 1592 1443 1347 1299 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4178 4210 4557 4589 6224 6256 10164 10196 4178
Size: 32 347 32 1635 32 3908 32 4186
Fragments arranged by size:
4186 3908 1635 347 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6791 1316
Size: 5475 4729
XbaI T'CTAG_A
Cuts at: 7540 7540
Size: 10204
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2462 3452 5017 5233 2462
Size: 990 1565 216 7433
Fragments arranged by size:
7433 1565 990 216
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10204
XmaI C'CCGG_G
Cuts at: 1870 4806 7529 1870
Size: 2936 2723 4545
Fragments arranged by size:
4545 2936 2723
XmnI GAAnn'nnTTC
Cuts at: 2916 4489 8333 2916
Size: 1573 3844 4787
Fragments arranged by size:
4787 3844 1573
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8012 276
Size: 53 83 186 936 6478 2468
Fragments arranged by size:
6478 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI BsiWI BstZ17I FseI FspAI NaeI
NgoMIV NruI PacI PmlI PspOMI PspXI SanDI SexAI
SfiI SrfI SwaI