Restriction map for
pFN21AB4908
(Circular) (Six-base) MAPSORT of: pFN21AB4908.seq Check: 8499 from: 1 to: 6637
pFN21AB4908 6637 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2453 2515 3063 3206 3786 2453
Size: 62 548 143 580 5304
Fragments arranged by size:
5304 580 548 143 62
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2526 4447 278
Size: 53 83 186 936 990 1921 2468
Fragments arranged by size:
2468 1921 990 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3980 1937
Size: 2043 4594
Acc65I G'GTAC_C
Cuts at: 3958 3958
Size: 6637
AclI AA'CG_TT
Cuts at: 1563 3936 4764 5137 1563
Size: 2373 828 373 3063
Fragments arranged by size:
3063 2373 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2378 2926 3010 3346 3430 3514 3598 4700
Size: 746 548 84 336 84 84 84 1102
Cuts at: 4700 6084 6352 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1102 746 548 336 268 84
84 84 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6440
AflIII A'CryG_T
Cuts at: 1203 2572 3372 5536 6513 1203
Size: 1369 800 2164 977 1327
Fragments arranged by size:
2164 1369 1327 977 800
AgeI A'CCGG_T
Cuts at: 3084 3572 5468 3084
Size: 488 1896 4253
Fragments arranged by size:
4253 1896 488
AhdI GACnn_n'nnGTC
Cuts at: 5366 5366
Size: 6637
AleI CACnn'nnGTG
Cuts at: 1166 3573 1166
Size: 2407 4230
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3056 3071 1184
Size: 1872 15 4750
Fragments arranged by size:
4750 1872 15
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2729 2761 3233 3265 2729
Size: 32 472 32 6101
Fragments arranged by size:
6101 472 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3012 3264 3733 4380 5952 3012
Size: 252 469 647 1572 3697
Fragments arranged by size:
3697 1572 647 469 252
ApaI G_GGCC'C
Cuts at: 2340 2340
Size: 6637
ApaLI G'TGCA_C
Cuts at: 2966 3062 3386 3470 3554 4694 5850 2966
Size: 96 324 84 84 1140 1156 3753
Fragments arranged by size:
3753 1156 1140 324 96 84 84
ApoI r'AATT_y
Cuts at: 1493 2537 3907 3946 4172 4324 6433 1493
Size: 1044 1370 39 226 152 2109 1697
Fragments arranged by size:
2109 1697 1370 1044 226 152 39
AseI AT'TA_AT
Cuts at: 160 1032 5191 160
Size: 872 4159 1606
Fragments arranged by size:
4159 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6637
AvaI C'yCGr_G
Cuts at: 1870 1943 2027 2081 2579 3962 1870
Size: 73 84 54 498 1383 4545
Fragments arranged by size:
4545 1383 498 84 73 54
AvrII C'CTAG_G
Cuts at: 2248 2248
Size: 6637
BamHI G'GATC_C
Cuts at: 2053 3844 3967 4334 2053
Size: 1791 123 367 4356
Fragments arranged by size:
4356 1791 367 123
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2365 2454 2497 2729 3958
Size: 325 217 627 578 89 43 232 1229
Cuts at: 3958 5413 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1229 627 578 325 232 217
89 43
BanII G_rGCy'C
Cuts at: 1391 2028 2340 2415 2786 3710 3956 1391
Size: 637 312 75 371 924 246 4072
Fragments arranged by size:
4072 924 637 371 312 246 75
BbeI G_GCGC'C
Cuts at: 2458 2501 2733 2458
Size: 43 232 6362
Fragments arranged by size:
6362 232 43
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2039 2096 928
Size: 971 140 57 5469
Fragments arranged by size:
5469 971 140 57
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4828 4862 1903
Size: 34 2891 34 3678
Fragments arranged by size:
3678 2891 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4529 5739 4529
Size: 1210 5427
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6637
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5248 136
Size: 107 122 71 631 605 3576 1525
Fragments arranged by size:
3576 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6632 6632
Size: 6637
BlpI GC'TnA_GC
Cuts at: 3044 3782 4054 3044
Size: 738 272 5627
Fragments arranged by size:
5627 738 272
Bme1580I G_kGCm'C
Cuts at: 1790 2340 2370 2970 3066 3390 3474 3558 4698
Size: 550 30 600 96 324 84 84 1140
Cuts at: 4698 5854 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1140 600 550 324 96 84
84 30
BmgBI CAC'GTC
Cuts at: 1318 3841 1318
Size: 2523 4114
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4368 5326 442
Size: 416 53 83 399 24 2951 958 1753
Fragments arranged by size:
2951 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6637
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3945 3945
Size: 6637
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2884 2900 3724 5297 1239
Size: 1645 16 824 1573 2579
Fragments arranged by size:
2579 1645 1573 824 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4762 5627 5925 4762
Size: 865 298 5474
Fragments arranged by size:
5474 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5300 882
Size: 617 3801 2219
Fragments arranged by size:
3801 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3796 4428 493
Size: 711 2592 632 2702
Fragments arranged by size:
2702 2592 711 632
BsaBI GATnn'nnATC
Cuts at: 6631 6631
Size: 6637
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2455 2498 2523 2730
Size: 53 83 186 936 922 43 25 207
Cuts at: 2730 4444 4826 5461 275
Size: 1714 382 635 1451
Fragments arranged by size:
1714 1451 936 922 635 382 207 186
83 53 43 25
BsaWI w'CCGG_w
Cuts at: 3084 3572 5070 5468 5742 5889 6300 3084
Size: 488 1498 398 274 147 411 3421
Fragments arranged by size:
3421 1498 488 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2729 2759 3653 3683 1178
Size: 30 165 30 1326 30 894 30 4132
Fragments arranged by size:
4132 1326 894 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2183 2315 2491 2776 1186
Size: 997 132 176 285 5047
Fragments arranged by size:
5047 997 285 176 132
BseYI C'CCAG_C
Cuts at: 2565 3811 5840 6336 2565
Size: 1246 2029 496 2866
Fragments arranged by size:
2866 2029 1246 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2246 2679 2783 3025 3277 3613 3719
Size: 573 510 433 104 242 252 336 106
Cuts at: 3719 3837 1163
Size: 118 3963
Fragments arranged by size:
3963 573 510 433 336 252 242 118
106 104
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4087 4848 5876 1540
Size: 275 186 2086 761 1028 2301
Fragments arranged by size:
2301 2086 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2470 2786 2870 2886 2970 3066 3390 3474 3558
Size: 316 84 16 84 96 324 84 84
Cuts at: 3558 3710 3726 4698 4783 5854 2470
Size: 152 16 972 85 1071 3253
Fragments arranged by size:
3253 1071 972 324 316 152 96 85
84 84 84 84 16 16
BsmI GAATG_Cn'
Cuts at: 2849 4148 4241 2849
Size: 1299 93 5245
Fragments arranged by size:
5245 1299 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2340 2370 2415 2470 2786 2870
Size: 399 238 312 30 45 55 316 84
Cuts at: 2870 2886 2970 3066 3390 3474 3558 3710 3726
Size: 16 84 96 324 84 84 152 16
Cuts at: 3726 3956 4698 4783 5854 1391
Size: 230 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 324 316 312 238
230 152 96 85 84 84 84 84
55 45 30 16 16
BspEI T'CCGG_A
Cuts at: 6300 6300
Size: 6637
BspHI T'CATG_A
Cuts at: 4526 6256 4526
Size: 1730 4907
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2453 2515 3063 3206 3387 3786 3992 844
Size: 1609 62 548 143 181 399 206 3489
Fragments arranged by size:
3489 1609 548 399 206 181 143 62
BsrBI CCG'CTC
Cuts at: 1394 1823 2425 2992 3288 4524 1394
Size: 429 602 567 296 1236 3507
Fragments arranged by size:
3507 1236 602 567 429 296
BsrDI GCAATG_nn'
Cuts at: 65 1248 5132 5306 65
Size: 1183 3884 174 1396
Fragments arranged by size:
3884 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2401 2712 2802 2886 2900 3068 3084
Size: 110 591 311 90 84 14 168 16
Cuts at: 3084 3390 3474 3572 5281 5468 1700
Size: 306 84 98 1709 187 2869
Fragments arranged by size:
2869 1709 591 311 306 187 168 110
98 90 84 84 16 14
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6637
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6637
BssSI C'ACGA_G
Cuts at: 3383 4697 5709 3383
Size: 1314 1012 4311
Fragments arranged by size:
4311 1314 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3012 3264 3432 3600 3824 794
Size: 2218 252 168 168 224 3607
Fragments arranged by size:
3607 2218 252 224 168 168
BstBI TT'CG_AA
Cuts at: 5441 5441
Size: 6637
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6637
BstXI CCAn_nnnn'nTGG
Cuts at: 2878 2878
Size: 6637
BstYI r'GATC_y
Cuts at: 1972 2010 2053 3228 3844 3967 4334 4719 4736
Size: 38 43 1175 616 123 367 385 17
Cuts at: 4736 6188 6274 6286 6632 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1175 616 385 367 346 123
86 43 38 17 12
Bsu36I CC'TnA_GG
Cuts at: 2546 2546
Size: 6637
BtgI C'CryG_G
Cuts at: 513 1065 2004 2306 2511 513
Size: 552 939 302 205 4639
Fragments arranged by size:
4639 939 552 302 205
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2853 487
Size: 683 56 1627 4271
Fragments arranged by size:
4271 1627 683 56
BtsI GCAGTG_nn'
Cuts at: 2258 4153 4947 4967 2258
Size: 1895 794 20 3928
Fragments arranged by size:
3928 1895 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6637
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6602
DraI TTT'AAA
Cuts at: 3941 3941
Size: 6637
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5644 809
Size: 507 4328 1802
Fragments arranged by size:
4328 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3756 4084 4973
Size: 54 1425 50 275 18 1926 328 889
Cuts at: 4973 6602 8
Size: 1629 43
Fragments arranged by size:
1926 1629 1425 889 328 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4084 1537
Size: 275 2272 4090
Fragments arranged by size:
4090 2272 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2564 4567 1353
Size: 1211 2003 3423
Fragments arranged by size:
3423 2003 1211
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2765 5224 5598 5744 387
Size: 1064 138 1176 2459 374 146 1280
Fragments arranged by size:
2459 1280 1176 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2784 3708 2784
Size: 924 5713
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2378 2884 2900 2926 3010 3346 3430
Size: 393 746 506 16 26 84 336 84
Cuts at: 3430 3514 3598 3724 4700 5297 6084 6352 1239
Size: 84 84 126 976 597 787 268 1524
Fragments arranged by size:
1524 976 787 746 597 506 393 336
268 126 84 84 84 84 26 16
EcoO109I rG'GnC_Cy
Cuts at: 2199 2252 2336 2337 2588 2641 2199
Size: 53 84 1 251 53 6195
Fragments arranged by size:
6195 251 84 53 53 1
EcoRI G'AATT_C
Cuts at: 2537 3946 4324 2537
Size: 1409 378 4850
Fragments arranged by size:
4850 1409 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2932 2932
Size: 6637
FspI TGC'GCA
Cuts at: 5143 5143
Size: 6637
HaeII r_GCGC'y
Cuts at: 1678 2458 2501 2733 2809 3012 5784 1678
Size: 780 43 232 76 203 2772 2531
Fragments arranged by size:
2772 2531 780 232 203 76 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2075 2107 2264 2269 2296
Size: 32 135 32 503 32 157 5 27
Cuts at: 2296 2301 5278 5310 5352 5384 1373
Size: 5 2977 32 42 32 2626
Fragments arranged by size:
2977 2626 503 157 135 42 32 32
32 32 32 27 5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2180 3981 4227 669
Size: 988 281 242 1801 246 3079
Fragments arranged by size:
3079 1801 988 281 246 242
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6637
HpaI GTT'AAC
Cuts at: 4227 4227
Size: 6637
KasI G'GCGC_C
Cuts at: 2454 2497 2729 2454
Size: 43 232 6362
Fragments arranged by size:
6362 232 43
KpnI G_GTAC'C
Cuts at: 3962 3962
Size: 6637
MfeI C'AATT_G
Cuts at: 4236 4236
Size: 6637
MluI A'CGCG_T
Cuts at: 2572 3372 6513 2572
Size: 800 3141 2696
Fragments arranged by size:
3141 2696 800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2202 3500 5751 5935 1305
Size: 897 1298 2251 184 2007
Fragments arranged by size:
2251 2007 1298 897 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3758 10
Size: 54 1425 343 1926 2889
Fragments arranged by size:
2889 1926 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3573 4595 4954 5113 518
Size: 648 2407 1022 359 159 2042
Fragments arranged by size:
2407 2042 1022 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2946 3030 3237 3366 3405 3534 4053 4730
Size: 1340 84 207 129 39 129 519 677
Cuts at: 4730 5878 6123 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1340 1148 677 519 245 207 129
129 84 39
NaeI GCC'GGC
Cuts at: 2714 2714
Size: 6637
NarI GG'CG_CC
Cuts at: 2455 2498 2730 2455
Size: 43 232 6362
Fragments arranged by size:
6362 232 43
NcoI C'CATG_G
Cuts at: 513 1065 2004 2306 2511 513
Size: 552 939 302 205 4639
Fragments arranged by size:
4639 939 552 302 205
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6637
NgoMIV G'CCGG_C
Cuts at: 2712 2712
Size: 6637
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6637
NotI GC'GGCC_GC
Cuts at: 4084 4084
Size: 6637
NspI r_CATG'y
Cuts at: 1132 1229 2317 3052 3995 5540 1132
Size: 97 1088 735 943 1545 2229
Fragments arranged by size:
2229 1545 1088 943 735 97
PasI CC'CwG_GG
Cuts at: 1348 2493 3916 1348
Size: 1145 1423 4069
Fragments arranged by size:
4069 1423 1145
PciI A'CATG_T
Cuts at: 5536 5536
Size: 6637
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2517 1108
Size: 1409 5228
PmeI GTTT'AAAC
Cuts at: 3941 3941
Size: 6637
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2729 2761 4706 4738 6219 6251 2729
Size: 32 1945 32 1481 32 3115
Fragments arranged by size:
3115 1945 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2252 2588 2252
Size: 336 6301
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6637
PsiI TTA'TAA
Cuts at: 4207 4207
Size: 6637
PspOMI G'GGCC_C
Cuts at: 2336 2336
Size: 6637
PstI C_TGCA'G
Cuts at: 830 2475 3035 3512 3989 830
Size: 1645 560 477 477 3478
Fragments arranged by size:
3478 1645 560 477 477
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6637
PvuII CAG'CTG
Cuts at: 2946 3030 3366 3534 2946
Size: 84 336 168 6049
Fragments arranged by size:
6049 336 168 84
SacI G_AGCT'C
Cuts at: 2786 3710 2786
Size: 924 5713
SalI G'TCGA_C
Cuts at: 1936 3979 1936
Size: 2043 4594
SanDI GG'GwC_CC
Cuts at: 2588 2588
Size: 6637
SbfI CC_TGCA'GG
Cuts at: 3989 3989
Size: 6637
ScaI AGT'ACT
Cuts at: 4885 6597 4885
Size: 1712 4925
SfcI C'TryA_G
Cuts at: 826 1045 2471 2529 3031 3508 3985 5120 5801
Size: 219 1426 58 502 477 477 1135 681
Cuts at: 5801 5992 826
Size: 191 1471
Fragments arranged by size:
1471 1426 1135 681 502 477 477 219
191 58
SfoI GGC'GCC
Cuts at: 2456 2499 2731 2456
Size: 43 232 6362
Fragments arranged by size:
6362 232 43
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6637
SgrAI Cr'CCGG_yG
Cuts at: 1700 2802 2886 2900 3068 3390 3474 3572 1700
Size: 1102 84 14 168 322 84 98 4765
Fragments arranged by size:
4765 1102 322 168 98 84 84 14
SmaI CCC'GGG
Cuts at: 1872 3964 1872
Size: 2092 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2081 4741 5642 5904 820
Size: 197 926 138 2660 901 262 1553
Fragments arranged by size:
2660 1553 926 901 262 197 138
SnaBI TAC'GTA
Cuts at: 493 4428 493
Size: 3935 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6637
SphI G_CATG'C
Cuts at: 1132 2317 3052 3995 1132
Size: 1185 735 943 3774
Fragments arranged by size:
3774 1185 943 735
SspI AAT'ATT
Cuts at: 5 52 4561 5
Size: 47 4509 2081
Fragments arranged by size:
4509 2081 47
StuI AGG'CCT
Cuts at: 1517 2855 2939 3023 3107 3191 3275 3527 1517
Size: 1338 84 84 84 84 84 252 4627
Fragments arranged by size:
4627 1338 252 84 84 84 84 84
StyI C'CwwG_G
Cuts at: 513 1065 2004 2121 2248 2306 2511 2637 3753
Size: 552 939 117 127 58 205 126 1116
Cuts at: 3753 4076 513
Size: 323 3074
Fragments arranged by size:
3074 1116 939 552 323 205 127 126
117 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2177 3803 4664 4681 4834 5019 5464
Size: 1255 597 1626 861 17 153 185 445
Cuts at: 5464 325
Size: 1498
Fragments arranged by size:
1626 1498 1255 861 597 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4883 6595 96
Size: 274 80 33 51 1443 2906 1712 138
Fragments arranged by size:
2906 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6597 6629 6597
Size: 32 6605
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6637
XbaI T'CTAG_A
Cuts at: 3973 3973
Size: 6637
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3856 3856
Size: 6637
XhoI C'TCGA_G
Cuts at: 1943 2081 1943
Size: 138 6499
XmaI C'CCGG_G
Cuts at: 1870 3962 1870
Size: 2092 4545
XmnI GAAnn'nnTTC
Cuts at: 2771 2912 2939 3023 3275 3332 3359 3416 3500
Size: 141 27 84 252 57 27 57 84
Cuts at: 3500 3527 4766 2771
Size: 27 1239 4642
Fragments arranged by size:
4642 1239 252 141 84 84 57 57
27 27 27
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2524 4445 276
Size: 53 83 186 936 990 1921 2468
Fragments arranged by size:
2468 1921 990 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI
BstZ17I DraIII EcoNI EcoRV FseI FspAI NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacII SapI
SexAI SfiI SrfI SwaI