Restriction map for
pFN21AB4907
(Circular) (Six-base) MAPSORT of: pFN21AB4907.seq Check: 1514 from: 1 to: 5911
pFN21AB4907 5911 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2279 3721 278
Size: 53 83 186 936 743 1442 2468
Fragments arranged by size:
2468 1442 936 743 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2592 3254 1937
Size: 655 662 4594
Fragments arranged by size:
4594 662 655
Acc65I G'GTAC_C
Cuts at: 3232 3232
Size: 5911
AclI AA'CG_TT
Cuts at: 1563 4038 4411 1563
Size: 2475 373 3063
Fragments arranged by size:
3063 2475 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2391 3026 3974 5358 5626 1632
Size: 759 635 948 1384 268 1917
Fragments arranged by size:
1917 1384 948 759 635 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5714
AflIII A'CryG_T
Cuts at: 1203 4810 5787 1203
Size: 3607 977 1327
Fragments arranged by size:
3607 1327 977
AgeI A'CCGG_T
Cuts at: 4742 4742
Size: 5911
AhdI GACnn_n'nnGTC
Cuts at: 4640 4640
Size: 5911
AleI CACnn'nnGTG
Cuts at: 1166 2958 1166
Size: 1792 4119
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5911
AlwNI CAG_nnn'CTG
Cuts at: 2321 3654 5226 2321
Size: 1333 1572 3006
Fragments arranged by size:
3006 1572 1333
ApaI G_GGCC'C
Cuts at: 2445 2445
Size: 5911
ApaLI G'TGCA_C
Cuts at: 3968 5124 3968
Size: 1156 4755
ApoI r'AATT_y
Cuts at: 1493 2524 3220 3446 3598 5707 1493
Size: 1031 696 226 152 2109 1697
Fragments arranged by size:
2109 1697 1031 696 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4465 160
Size: 872 3433 1606
Fragments arranged by size:
3433 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5911
AvaI C'yCGr_G
Cuts at: 1870 1943 2688 3236 1870
Size: 73 745 548 4545
Fragments arranged by size:
4545 745 548 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2385 2418 2804 2837 3034 3067 2385
Size: 33 386 33 197 33 5229
Fragments arranged by size:
5229 386 197 33 33 33
BamHI G'GATC_C
Cuts at: 3241 3608 3241
Size: 367 5544
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2581 3232 4687 618
Size: 325 217 627 794 651 1455 1842
Fragments arranged by size:
1842 1455 794 651 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2199 2445 3230 1391
Size: 808 246 785 4072
Fragments arranged by size:
4072 808 785 246
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2352 928
Size: 971 453 4487
Fragments arranged by size:
4487 971 453
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4102 4136 1903
Size: 34 2165 34 3678
Fragments arranged by size:
3678 2165 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2960 3803 5013 2960
Size: 843 1210 3858
Fragments arranged by size:
3858 1210 843
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5911
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4522 136
Size: 107 122 71 631 605 2850 1525
Fragments arranged by size:
2850 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5906 5906
Size: 5911
BlpI GC'TnA_GC
Cuts at: 3328 3328
Size: 5911
Bme1580I G_kGCm'C
Cuts at: 1790 2445 3972 5128 1790
Size: 655 1527 1156 2573
Fragments arranged by size:
2573 1527 1156 655
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5911
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2297 2819 3642
Size: 416 53 83 399 24 880 522 823
Cuts at: 3642 4600 442
Size: 958 1753
Fragments arranged by size:
1753 958 880 823 522 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5911
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2421 2920 4571 1239
Size: 1182 499 1651 2579
Fragments arranged by size:
2579 1651 1182 499
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4036 4901 5199 4036
Size: 865 298 4748
Fragments arranged by size:
4748 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2133 4574 882
Size: 617 634 2441 2219
Fragments arranged by size:
2441 2219 634 617
BsaAI yAC'GTr
Cuts at: 493 1204 3702 493
Size: 711 2498 2702
Fragments arranged by size:
2702 2498 711
BsaBI GATnn'nnATC
Cuts at: 5905 5905
Size: 5911
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2276 2908 3718 4100
Size: 53 83 186 936 743 632 810 382
Cuts at: 4100 4735 275
Size: 635 1451
Fragments arranged by size:
1451 936 810 743 635 632 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3015 4344 4742 5016 5163 5574 3015
Size: 1329 398 274 147 411 3352
Fragments arranged by size:
3352 1329 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2195 2225 1178
Size: 30 165 30 792 30 4864
Fragments arranged by size:
4864 792 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2317 1186
Size: 1131 4780
BseYI C'CCAG_C
Cuts at: 5114 5610 5114
Size: 496 5415
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3049 3170 1163
Size: 573 1313 121 3904
Fragments arranged by size:
3904 1313 573 121
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3361 4122 5150 1540
Size: 275 186 1360 761 1028 2301
Fragments arranged by size:
2301 1360 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2903 3972 4057 5128 2903
Size: 1069 85 1071 3686
Fragments arranged by size:
3686 1071 1069 85
BsmI GAATG_Cn'
Cuts at: 3422 3515 3422
Size: 93 5818
BsmBI CGTCTCn'nnnn_
Cuts at: 2908 2908
Size: 5911
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2199 2445 2903 3230 3972 4057 5128
Size: 399 409 246 458 327 742 85 1071
Cuts at: 5128 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 458 409 399 327 246
85
BspEI T'CCGG_A
Cuts at: 5574 5574
Size: 5911
BspHI T'CATG_A
Cuts at: 3800 5530 3800
Size: 1730 4181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2745 3266 844
Size: 1901 521 3489
Fragments arranged by size:
3489 1901 521
BsrBI CCG'CTC
Cuts at: 1394 1823 3798 1394
Size: 429 1975 3507
Fragments arranged by size:
3507 1975 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4406 4580 65
Size: 1183 3158 174 1396
Fragments arranged by size:
3158 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2083 4555 4742 1700
Size: 110 273 2472 187 2869
Fragments arranged by size:
2869 2472 273 187 110
BsrGI T'GTAC_A
Cuts at: 96 2985 96
Size: 2889 3022
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5911
BssSI C'ACGA_G
Cuts at: 3971 4983 3971
Size: 1012 4899
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2485 794
Size: 1691 4220
BstBI TT'CG_AA
Cuts at: 4715 4715
Size: 5911
BstEII G'GTnAC_C
Cuts at: 1184 2607 1184
Size: 1423 4488
BstYI r'GATC_y
Cuts at: 1972 2774 3241 3608 3993 4010 5462 5548 5560
Size: 802 467 367 385 17 1452 86 12
Cuts at: 5560 5906 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 802 467 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2565 2665 513
Size: 552 1500 100 3759
Fragments arranged by size:
3759 1500 552 100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5172
Fragments arranged by size:
5172 683 56
BtsI GCAGTG_nn'
Cuts at: 3427 4221 4241 3427
Size: 794 20 5097
Fragments arranged by size:
5097 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5911
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5876
DraI TTT'AAA
Cuts at: 3215 3215
Size: 5911
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4918 809
Size: 507 3602 1802
Fragments arranged by size:
3602 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3358 4247 5876
Size: 54 1425 50 275 18 1528 889 1629
Cuts at: 5876 8
Size: 43
Fragments arranged by size:
1629 1528 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3358 1537
Size: 275 1546 4090
Fragments arranged by size:
4090 1546 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2015 2356 3841 1353
Size: 662 341 1485 3423
Fragments arranged by size:
3423 1485 662 341
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4498 4872 5018 387
Size: 1064 138 2909 374 146 1280
Fragments arranged by size:
2909 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2391 2421 2920 3026 3974 4571 5358
Size: 393 759 30 499 106 948 597 787
Cuts at: 5358 5626 1239
Size: 268 1524
Fragments arranged by size:
1524 948 787 759 597 499 393 268
106 30
EcoO109I rG'GnC_Cy
Cuts at: 2441 2442 2441
Size: 1 5910
EcoRI G'AATT_C
Cuts at: 3220 3598 3220
Size: 378 5533
FspI TGC'GCA
Cuts at: 4417 4417
Size: 5911
HaeII r_GCGC'y
Cuts at: 1678 5058 1678
Size: 3380 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2198 2230 4552 4584 4626
Size: 32 135 32 626 32 2322 32 42
Cuts at: 4626 4658 1373
Size: 32 2626
Fragments arranged by size:
2626 2322 626 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2424 3255 3501 669
Size: 988 281 486 831 246 3079
Fragments arranged by size:
3079 988 831 486 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5911
HpaI GTT'AAC
Cuts at: 3501 3501
Size: 5911
KpnI G_GTAC'C
Cuts at: 3236 3236
Size: 5911
MfeI C'AATT_G
Cuts at: 3510 3510
Size: 5911
MluI A'CGCG_T
Cuts at: 5787 5787
Size: 5911
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2449 5025 5209 1305
Size: 1144 2576 184 2007
Fragments arranged by size:
2576 2007 1144 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4089
Fragments arranged by size:
4089 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2564 2958 3136 3869 4228 4387 518
Size: 648 1398 394 178 733 359 159 2042
Fragments arranged by size:
2042 1398 733 648 394 359 178 159
MspA1I CmG'CkG
Cuts at: 1606 2600 3327 4004 5152 5397 1606
Size: 994 727 677 1148 245 2120
Fragments arranged by size:
2120 1148 994 727 677 245
NaeI GCC'GGC
Cuts at: 2085 2085
Size: 5911
NcoI C'CATG_G
Cuts at: 513 1065 2665 513
Size: 552 1600 3759
Fragments arranged by size:
3759 1600 552
NdeI CA'TA_TG
Cuts at: 387 2016 3201 387
Size: 1629 1185 3097
Fragments arranged by size:
3097 1629 1185
NgoMIV G'CCGG_C
Cuts at: 2083 2083
Size: 5911
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5911
NotI GC'GGCC_GC
Cuts at: 3358 3358
Size: 5911
NspI r_CATG'y
Cuts at: 1132 1229 3269 4814 1132
Size: 97 2040 1545 2229
Fragments arranged by size:
2229 2040 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5911
PciI A'CATG_T
Cuts at: 4810 4810
Size: 5911
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5911
PmeI GTTT'AAAC
Cuts at: 3215 3215
Size: 5911
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3980 4012 5493 5525 3980
Size: 32 1481 32 4366
Fragments arranged by size:
4366 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5911
PsiI TTA'TAA
Cuts at: 3481 3481
Size: 5911
PspOMI G'GGCC_C
Cuts at: 2441 2441
Size: 5911
PstI C_TGCA'G
Cuts at: 830 3263 830
Size: 2433 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5911
PvuII CAG'CTG
Cuts at: 2600 2600
Size: 5911
SalI G'TCGA_C
Cuts at: 1936 3253 1936
Size: 1317 4594
SbfI CC_TGCA'GG
Cuts at: 3263 3263
Size: 5911
ScaI AGT'ACT
Cuts at: 4159 5871 4159
Size: 1712 4199
SfcI C'TryA_G
Cuts at: 826 1045 2217 3259 4394 5075 5266 826
Size: 219 1172 1042 1135 681 191 1471
Fragments arranged by size:
1471 1172 1135 1042 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5911
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5911
SmaI CCC'GGG
Cuts at: 1872 3238 1872
Size: 1366 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4015 4916 5178 820
Size: 197 926 2072 901 262 1553
Fragments arranged by size:
2072 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3702 493
Size: 3209 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5911
SphI G_CATG'C
Cuts at: 1132 3269 1132
Size: 2137 3774
SspI AAT'ATT
Cuts at: 5 52 3835 5
Size: 47 3783 2081
Fragments arranged by size:
3783 2081 47
StuI AGG'CCT
Cuts at: 1517 2694 1517
Size: 1177 4734
StyI C'CwwG_G
Cuts at: 513 1065 2665 3350 513
Size: 552 1600 685 3074
Fragments arranged by size:
3074 1600 685 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3938 3955 4108 4293 4738 325
Size: 1255 2358 17 153 185 445 1498
Fragments arranged by size:
2358 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2803 2985 4157
Size: 274 80 33 51 1443 826 182 1172
Cuts at: 4157 5869 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1172 826 274 182 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5871 5903 5871
Size: 32 5879
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5911
XbaI T'CTAG_A
Cuts at: 3247 3247
Size: 5911
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5911
XmaI C'CCGG_G
Cuts at: 1870 3236 1870
Size: 1366 4545
XmnI GAAnn'nnTTC
Cuts at: 2163 3131 4040 2163
Size: 968 909 4034
Fragments arranged by size:
4034 968 909
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2277 3719 276
Size: 53 83 186 936 743 1442 2468
Fragments arranged by size:
2468 1442 936 743 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI