Restriction map for
pFN21AB4905
(Circular) (Six-base) MAPSORT of: pFN21AB4905.seq Check: 9922 from: 1 to: 5761
pFN21AB4905 5761 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3571 278
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3104 1937
Size: 1167 4594
Acc65I G'GTAC_C
Cuts at: 2517 3082 2517
Size: 565 5196
AclI AA'CG_TT
Cuts at: 1563 3060 3888 4261 1563
Size: 1497 828 373 3063
Fragments arranged by size:
3063 1497 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3824 5208 5476 1632
Size: 2192 1384 268 1917
Fragments arranged by size:
2192 1917 1384 268
AfeI AGC'GCT
Cuts at: 2153 2153
Size: 5761
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5564
AflIII A'CryG_T
Cuts at: 1203 2859 4660 5637 1203
Size: 1656 1801 977 1327
Fragments arranged by size:
1801 1656 1327 977
AgeI A'CCGG_T
Cuts at: 4592 4592
Size: 5761
AhdI GACnn_n'nnGTC
Cuts at: 4490 4490
Size: 5761
AleI CACnn'nnGTG
Cuts at: 1166 2477 1166
Size: 1311 4450
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2495 1184
Size: 1311 4450
AlwNI CAG_nnn'CTG
Cuts at: 3504 5076 3504
Size: 1572 4189
ApaLI G'TGCA_C
Cuts at: 3818 4974 3818
Size: 1156 4605
ApoI r'AATT_y
Cuts at: 1493 3070 3296 3448 5557 1493
Size: 1577 226 152 2109 1697
Fragments arranged by size:
2109 1697 1577 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4315 160
Size: 872 3283 1606
Fragments arranged by size:
3283 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
AvaI C'yCGr_G
Cuts at: 1870 1943 3086 1870
Size: 73 1143 4545
Fragments arranged by size:
4545 1143 73
BamHI G'GATC_C
Cuts at: 3091 3458 3091
Size: 367 5394
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2246 2517 2532 3082 4537
Size: 325 217 627 459 271 15 550 1455
Cuts at: 4537 618
Size: 1842
Fragments arranged by size:
1842 1455 627 550 459 325 271 217
15
BanII G_rGCy'C
Cuts at: 1391 2778 2933 3006 3080 1391
Size: 1387 155 73 74 4072
Fragments arranged by size:
4072 1387 155 74 73
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4790
BbvCI CC'TCA_GC
Cuts at: 2733 2733
Size: 5761
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3952 3986 1903
Size: 34 2015 34 3678
Fragments arranged by size:
3678 2015 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2141 3653 4863 2141
Size: 1512 1210 3039
Fragments arranged by size:
3039 1512 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5761
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2198 2661 2759
Size: 107 122 71 631 605 526 463 98
Cuts at: 2759 2958 4372 136
Size: 199 1414 1525
Fragments arranged by size:
1525 1414 631 605 526 463 199 122
107 98 71
BglII A'GATC_T
Cuts at: 5756 5756
Size: 5761
BlpI GC'TnA_GC
Cuts at: 2792 3178 2792
Size: 386 5375
Bme1580I G_kGCm'C
Cuts at: 1790 2484 2535 2927 3822 4978 1790
Size: 694 51 392 895 1156 2573
Fragments arranged by size:
2573 1156 895 694 392 51
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5761
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3492 4450 442
Size: 416 53 83 399 24 2075 958 1753
Fragments arranged by size:
2075 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5761
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2071 2071
Size: 5761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2272 2939 3016 3020 4421 1239
Size: 1033 667 77 4 1401 2579
Fragments arranged by size:
2579 1401 1033 667 77 4
Bpu10I CC'TnA_GC
Cuts at: 2733 2831 2733
Size: 98 5663
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3026 3886 4751 5049 3026
Size: 860 865 298 3738
Fragments arranged by size:
3738 865 860 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4424 882
Size: 617 2925 2219
Fragments arranged by size:
2925 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2719 3552 493
Size: 711 1515 833 2702
Fragments arranged by size:
2702 1515 833 711
BsaBI GATnn'nnATC
Cuts at: 5755 5755
Size: 5761
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2620 2939 3568 3950
Size: 53 83 186 936 1087 319 629 382
Cuts at: 3950 4585 275
Size: 635 1451
Fragments arranged by size:
1451 1087 936 635 629 382 319 186
83 53
BsaWI w'CCGG_w
Cuts at: 4194 4592 4866 5013 5424 4194
Size: 398 274 147 411 4531
Fragments arranged by size:
4531 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5536
Fragments arranged by size:
5536 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5761
BseYI C'CCAG_C
Cuts at: 2522 4964 5460 2522
Size: 2442 496 2823
Fragments arranged by size:
2823 2442 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5188
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2665 3211 3972 5000 1540
Size: 275 186 664 546 761 1028 2301
Fragments arranged by size:
2301 1028 761 664 546 275 186
BsiHKAI G_wGCw'C
Cuts at: 2679 2814 3822 3907 4978 2679
Size: 135 1008 85 1071 3462
Fragments arranged by size:
3462 1071 1008 135 85
BsmI GAATG_Cn'
Cuts at: 3272 3365 3272
Size: 93 5668
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2484 2535 2679 2778 2814 2927 2933
Size: 399 694 51 144 99 36 113 6
Cuts at: 2933 3006 3080 3822 3907 4978 1391
Size: 73 74 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 694 399 144 113 99
85 74 73 51 36 6
BspEI T'CCGG_A
Cuts at: 5424 5424
Size: 5761
BspHI T'CATG_A
Cuts at: 3650 5380 3650
Size: 1730 4031
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3116 844
Size: 2272 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2349 2985 2996 3648 1394
Size: 429 526 636 11 652 3507
Fragments arranged by size:
3507 652 636 526 429 11
BsrDI GCAATG_nn'
Cuts at: 65 1248 4256 4430 65
Size: 1183 3008 174 1396
Fragments arranged by size:
3008 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2660 2753 2867 4405 4592 1700
Size: 110 850 93 114 1538 187 2869
Fragments arranged by size:
2869 1538 850 187 114 110 93
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5761
BssHII G'CGCG_C
Cuts at: 1925 2745 1925
Size: 820 4941
BssSI C'ACGA_G
Cuts at: 2777 3821 4833 2777
Size: 1044 1012 3705
Fragments arranged by size:
3705 1044 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5761
BstBI TT'CG_AA
Cuts at: 4565 4565
Size: 5761
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5761
BstXI CCAn_nnnn'nTGG
Cuts at: 2401 2917 2401
Size: 516 5245
BstYI r'GATC_y
Cuts at: 1972 3091 3458 3843 3860 5312 5398 5410 5756
Size: 1119 367 385 17 1452 86 12 346
Cuts at: 5756 1972
Size: 1977
Fragments arranged by size:
1977 1452 1119 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2513 2953 513
Size: 552 1448 440 3321
Fragments arranged by size:
3321 1448 552 440
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2414 2800 487
Size: 683 56 1188 386 3448
Fragments arranged by size:
3448 1188 683 386 56
BtsI GCAGTG_nn'
Cuts at: 2160 2211 3024 3277 4071 4091 2160
Size: 51 813 253 794 20 3830
Fragments arranged by size:
3830 813 794 253 51 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5761
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5726
DraI TTT'AAA
Cuts at: 3065 3065
Size: 5761
DraIII CAC_nnn'GTG
Cuts at: 2042 2722 2042
Size: 680 5081
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4768 809
Size: 507 3452 1802
Fragments arranged by size:
3452 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2190 2199 2331
Size: 54 1425 50 275 18 360 9 132
Cuts at: 2331 2448 2598 2662 2751 2755 3208 4097 5726
Size: 117 150 64 89 4 453 889 1629
Cuts at: 5726 8
Size: 43
Fragments arranged by size:
1629 1425 889 453 360 275 150 132
117 89 64 54 50 43 18 9
4
EagI C'GGCC_G
Cuts at: 1537 1812 2662 3208 1537
Size: 275 850 546 4090
Fragments arranged by size:
4090 850 546 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3691 1353
Size: 2338 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2633 4348 4722 4868 387
Size: 1064 138 1044 1715 374 146 1280
Fragments arranged by size:
1715 1280 1064 1044 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2272 2939 3016 3020 3824 4421 5208
Size: 393 640 667 77 4 804 597 787
Cuts at: 5208 5476 1239
Size: 268 1524
Fragments arranged by size:
1524 804 787 667 640 597 393 268
77 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2732 2732
Size: 5761
EcoRI G'AATT_C
Cuts at: 3070 3448 3070
Size: 378 5383
FseI GG_CCGG'CC
Cuts at: 2757 2757
Size: 5761
FspI TGC'GCA
Cuts at: 4267 4267
Size: 5761
HaeII r_GCGC'y
Cuts at: 1678 2090 2155 2641 4908 1678
Size: 412 65 486 2267 2531
Fragments arranged by size:
2531 2267 486 412 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4402 4434 4476 4508 1373
Size: 32 135 32 2830 32 42 32 2626
Fragments arranged by size:
2830 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3105 3351 669
Size: 988 281 1167 246 3079
Fragments arranged by size:
3079 1167 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5761
HpaI GTT'AAC
Cuts at: 3351 3351
Size: 5761
KpnI G_GTAC'C
Cuts at: 2521 3086 2521
Size: 565 5196
MfeI C'AATT_G
Cuts at: 3360 3360
Size: 5761
MluI A'CGCG_T
Cuts at: 2859 5637 2859
Size: 2778 2983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4875 5059 1305
Size: 3570 184 2007
Fragments arranged by size:
3570 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2450 2600 10
Size: 54 1425 343 618 150 3171
Fragments arranged by size:
3171 1425 618 343 150 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2333 2429 2477 3719 4078 4237 518
Size: 648 1167 96 48 1242 359 159 2042
Fragments arranged by size:
2042 1242 1167 648 359 159 96 48
MspA1I CmG'CkG
Cuts at: 1606 2515 2955 3177 3854 5002 5247 1606
Size: 909 440 222 677 1148 245 2120
Fragments arranged by size:
2120 1148 909 677 440 245 222
NaeI GCC'GGC
Cuts at: 2755 2869 2755
Size: 114 5647
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5209
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5761
NgoMIV G'CCGG_C
Cuts at: 2753 2867 2753
Size: 114 5647
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5761
NotI GC'GGCC_GC
Cuts at: 3208 3208
Size: 5761
NspI r_CATG'y
Cuts at: 1132 1229 2308 3119 4664 1132
Size: 97 1079 811 1545 2229
Fragments arranged by size:
2229 1545 1079 811 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5761
PciI A'CATG_T
Cuts at: 4660 4660
Size: 5761
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5761
PmeI GTTT'AAAC
Cuts at: 3065 3065
Size: 5761
PmlI CAC'GTG
Cuts at: 2719 2719
Size: 5761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3830 3862 5343 5375 3830
Size: 32 1481 32 4216
Fragments arranged by size:
4216 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5761
PsiI TTA'TAA
Cuts at: 3331 3331
Size: 5761
PstI C_TGCA'G
Cuts at: 830 2026 3113 830
Size: 1196 1087 3478
Fragments arranged by size:
3478 1196 1087
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5761
SacII CC_GC'GG
Cuts at: 2516 2956 2516
Size: 440 5321
SalI G'TCGA_C
Cuts at: 1936 3103 1936
Size: 1167 4594
SbfI CC_TGCA'GG
Cuts at: 3113 3113
Size: 5761
ScaI AGT'ACT
Cuts at: 4009 5721 4009
Size: 1712 4049
SfcI C'TryA_G
Cuts at: 826 1045 2022 2627 3109 4244 4925 5116 826
Size: 219 977 605 482 1135 681 191 1471
Fragments arranged by size:
1471 1135 977 681 605 482 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2198 2759 2198
Size: 561 5200
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
SgrAI Cr'CCGG_yG
Cuts at: 1700 2867 1700
Size: 1167 4594
SmaI CCC'GGG
Cuts at: 1872 3088 1872
Size: 1216 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3041 3865 4766 5028 820
Size: 197 926 1098 824 901 262 1553
Fragments arranged by size:
1553 1098 926 901 824 262 197
SnaBI TAC'GTA
Cuts at: 493 3552 493
Size: 3059 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5761
SphI G_CATG'C
Cuts at: 1132 3119 1132
Size: 1987 3774
SspI AAT'ATT
Cuts at: 5 52 3685 5
Size: 47 3633 2081
Fragments arranged by size:
3633 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5761
StyI C'CwwG_G
Cuts at: 513 1065 2242 2394 3200 513
Size: 552 1177 152 806 3074
Fragments arranged by size:
3074 1177 806 552 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2419 3788 3805 3958 4143 4588 325
Size: 1255 839 1369 17 153 185 445 1498
Fragments arranged by size:
1498 1369 1255 839 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4007 5719 96
Size: 274 80 33 51 1443 2030 1712 138
Fragments arranged by size:
2030 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2811 2843 5721 5753 2811
Size: 32 2878 32 2819
Fragments arranged by size:
2878 2819 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5761
XbaI T'CTAG_A
Cuts at: 2341 3097 2341
Size: 756 5005
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5761
XmaI C'CCGG_G
Cuts at: 1870 3086 1870
Size: 1216 4545
XmnI GAAnn'nnTTC
Cuts at: 3890 3890
Size: 5761
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3569 276
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PstI PvuI SacII SalI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FalI
FspAI KasI NarI NruI NsiI PacI PfoI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI
SexAI SfoI SrfI SwaI XcmI