Restriction map for
pFN21AB4902
(Circular) (Six-base) MAPSORT of: pFN21AB4902.seq Check: 5159 from: 1 to: 6937
pFN21AB4902 6937 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4747 278
Size: 53 83 186 936 3211 2468
Fragments arranged by size:
3211 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3276 4280 1937
Size: 1339 1004 4594
Fragments arranged by size:
4594 1339 1004
Acc65I G'GTAC_C
Cuts at: 4258 4258
Size: 6937
AclI AA'CG_TT
Cuts at: 1563 5064 5437 1563
Size: 3501 373 3063
Fragments arranged by size:
3501 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2171 2504 2952 3944 5000 6384 6652 1632
Size: 539 333 448 992 1056 1384 268 1917
Fragments arranged by size:
1917 1384 1056 992 539 448 333 268
AflII C'TTAA_G
Cuts at: 820 1017 4201 820
Size: 197 3184 3556
Fragments arranged by size:
3556 3184 197
AflIII A'CryG_T
Cuts at: 1203 2758 5836 6813 1203
Size: 1555 3078 977 1327
Fragments arranged by size:
3078 1555 1327 977
AgeI A'CCGG_T
Cuts at: 5768 5768
Size: 6937
AhdI GACnn_n'nnGTC
Cuts at: 5666 5666
Size: 6937
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6937
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6937
AlwNI CAG_nnn'CTG
Cuts at: 3067 4680 6252 3067
Size: 1613 1572 3752
Fragments arranged by size:
3752 1613 1572
ApaLI G'TGCA_C
Cuts at: 4994 6150 4994
Size: 1156 5781
ApoI r'AATT_y
Cuts at: 1493 2363 2572 2681 3307 4187 4246 4472 4624
Size: 870 209 109 626 880 59 226 152
Cuts at: 4624 6733 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 880 870 626 226 209 152
109 59
AseI AT'TA_AT
Cuts at: 160 1032 5491 160
Size: 872 4459 1606
Fragments arranged by size:
4459 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6937
AvaI C'yCGr_G
Cuts at: 1870 1943 2715 3549 4262 1870
Size: 73 772 834 713 4545
Fragments arranged by size:
4545 834 772 713 73
BamHI G'GATC_C
Cuts at: 2470 4165 4267 4634 2470
Size: 1695 102 367 4773
Fragments arranged by size:
4773 1695 367 102
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3901 4258 5713 618
Size: 325 217 627 2114 357 1455 1842
Fragments arranged by size:
2114 1842 1455 627 357 325 217
BanII G_rGCy'C
Cuts at: 1391 2850 4256 1391
Size: 1459 1406 4072
Fragments arranged by size:
4072 1459 1406
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2159 3172 3656 928
Size: 971 260 1013 484 4209
Fragments arranged by size:
4209 1013 971 484 260
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2556 2590 5128 5162 1903
Size: 34 619 34 2538 34 3678
Fragments arranged by size:
3678 2538 619 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4829 6039 4829
Size: 1210 5727
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6937
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5548 136
Size: 107 122 71 631 605 3876 1525
Fragments arranged by size:
3876 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3133 3702 3765 6932 3133
Size: 569 63 3167 3138
Fragments arranged by size:
3167 3138 569 63
BlpI GC'TnA_GC
Cuts at: 4354 4354
Size: 6937
Bme1580I G_kGCm'C
Cuts at: 1790 4008 4998 6154 1790
Size: 2218 990 1156 2573
Fragments arranged by size:
2573 2218 1156 990
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6937
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3343 3536 4668
Size: 416 53 83 399 24 1926 193 1132
Cuts at: 4668 5626 442
Size: 958 1753
Fragments arranged by size:
1926 1753 1132 958 416 399 193 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 4221 1056
Size: 3165 3772
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2139 2139
Size: 6937
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2712 5597 1239
Size: 1473 2885 2579
Fragments arranged by size:
2885 2579 1473
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5062 5927 6225 5062
Size: 865 298 5774
Fragments arranged by size:
5774 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3293 5600 882
Size: 617 1794 2307 2219
Fragments arranged by size:
2307 2219 1794 617
BsaAI yAC'GTr
Cuts at: 493 1204 4003 4728 493
Size: 711 2799 725 2702
Fragments arranged by size:
2799 2702 725 711
BsaBI GATnn'nnATC
Cuts at: 4121 6931 4121
Size: 2810 4127
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4744 5126 5761 275
Size: 53 83 186 936 3211 382 635 1451
Fragments arranged by size:
3211 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5370 5768 6042 6189 6600 5370
Size: 398 274 147 411 5707
Fragments arranged by size:
5707 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2938 2968 1178
Size: 30 165 30 1535 30 5147
Fragments arranged by size:
5147 1535 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2951 3450 3627 3897 1186
Size: 1765 499 177 270 4226
Fragments arranged by size:
4226 1765 499 270 177
BseYI C'CCAG_C
Cuts at: 2213 3789 6140 6636 2213
Size: 1576 2351 496 2514
Fragments arranged by size:
2514 2351 1576 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2088 3599 1163
Size: 573 352 1511 4501
Fragments arranged by size:
4501 1511 573 352
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4387 5148 6176 1540
Size: 275 186 2386 761 1028 2301
Fragments arranged by size:
2386 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4998 5083 6154 4998
Size: 85 1071 5781
Fragments arranged by size:
5781 1071 85
BsmI GAATG_Cn'
Cuts at: 2186 2842 4448 4541 2186
Size: 656 1606 93 4582
Fragments arranged by size:
4582 1606 656 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2850 4008 4256 4998 5083 6154 1391
Size: 399 1060 1158 248 742 85 1071 2174
Fragments arranged by size:
2174 1158 1071 1060 742 399 248 85
BspEI T'CCGG_A
Cuts at: 6600 6600
Size: 6937
BspHI T'CATG_A
Cuts at: 3043 4826 6556 3043
Size: 1783 1730 3424
Fragments arranged by size:
3424 1783 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4292 844
Size: 3448 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4824 1394
Size: 429 3001 3507
Fragments arranged by size:
3507 3001 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2181 4011 5432 5606 65
Size: 1183 933 1830 1421 174 1396
Fragments arranged by size:
1830 1421 1396 1183 933 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5581 5768 1700
Size: 110 3771 187 2869
Fragments arranged by size:
3771 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6937
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6937
BssSI C'ACGA_G
Cuts at: 4997 6009 4997
Size: 1012 5925
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3671 794
Size: 2877 4060
BstBI TT'CG_AA
Cuts at: 5741 5741
Size: 6937
BstEII G'GTnAC_C
Cuts at: 1184 3536 1184
Size: 2352 4585
BstYI r'GATC_y
Cuts at: 1972 2470 3133 3702 3765 4165 4267 4634 5019
Size: 498 663 569 63 400 102 367 385
Cuts at: 5019 5036 6488 6574 6586 6932 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 663 569 498 400 385 367
346 102 86 63 17 12
Bsu36I CC'TnA_GG
Cuts at: 3909 3909
Size: 6937
BtgI C'CryG_G
Cuts at: 513 1065 2004 2466 3294 513
Size: 552 939 462 828 4156
Fragments arranged by size:
4156 939 828 552 462
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6198
Fragments arranged by size:
6198 683 56
BtsI GCAGTG_nn'
Cuts at: 3354 4453 5247 5267 3354
Size: 1099 794 20 5024
Fragments arranged by size:
5024 1099 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6937
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6902
DraI TTT'AAA
Cuts at: 4241 4241
Size: 6937
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3053 5944 809
Size: 507 1737 2891 1802
Fragments arranged by size:
2891 1802 1737 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2098 3291 4384
Size: 54 1425 50 275 18 268 1193 1093
Cuts at: 4384 5273 6902 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1193 1093 889 275 268 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4384 1537
Size: 275 2572 4090
Fragments arranged by size:
4090 2572 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2928 3156 3885 4867 1353
Size: 1575 228 729 982 3423
Fragments arranged by size:
3423 1575 982 729 228
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5524 5898 6044 387
Size: 1064 138 3935 374 146 1280
Fragments arranged by size:
3935 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2171 2504 2712 2952 3944 5000 5597
Size: 393 539 333 208 240 992 1056 597
Cuts at: 5597 6384 6652 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1056 992 787 597 539 393 333
268 240 208
EcoNI CCTnn'n_nnAGG
Cuts at: 2499 2499
Size: 6937
EcoO109I rG'GnC_Cy
Cuts at: 2118 3632 2118
Size: 1514 5423
EcoRI G'AATT_C
Cuts at: 4246 4624 4246
Size: 378 6559
EcoRV GAT'ATC
Cuts at: 3466 3466
Size: 6937
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2824 3784 3974 2824
Size: 960 190 5787
Fragments arranged by size:
5787 960 190
FspI TGC'GCA
Cuts at: 5443 5443
Size: 6937
HaeII r_GCGC'y
Cuts at: 1678 6084 1678
Size: 4406 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2036 2068 2078 2110 3238
Size: 32 135 32 464 32 10 32 1128
Cuts at: 3238 3270 3694 3726 5578 5610 5652 5684 1373
Size: 32 424 32 1852 32 42 32 2626
Fragments arranged by size:
2626 1852 1128 464 424 135 42 32
32 32 32 32 32 32 32 10
HincII GTy'rAC
Cuts at: 669 1657 1938 3058 4281 4527 669
Size: 988 281 1120 1223 246 3079
Fragments arranged by size:
3079 1223 1120 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2432 3920 4038 748
Size: 1684 1488 118 3647
Fragments arranged by size:
3647 1684 1488 118
HpaI GTT'AAC
Cuts at: 4527 4527
Size: 6937
KpnI G_GTAC'C
Cuts at: 4262 4262
Size: 6937
MfeI C'AATT_G
Cuts at: 2062 4083 4536 2062
Size: 2021 453 4463
Fragments arranged by size:
4463 2021 453
MluI A'CGCG_T
Cuts at: 6813 6813
Size: 6937
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2355 2518 2722 3708 4014 6051 6235 1305
Size: 1050 163 204 986 306 2037 184 2007
Fragments arranged by size:
2037 2007 1050 986 306 204 184 163
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2100 10
Size: 54 1425 343 268 4847
Fragments arranged by size:
4847 1425 343 268 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3116 3359 4001 4895 5254 5413 518
Size: 648 1950 243 642 894 359 159 2042
Fragments arranged by size:
2042 1950 894 648 642 359 243 159
MspA1I CmG'CkG
Cuts at: 1606 4030 4353 5030 6178 6423 1606
Size: 2424 323 677 1148 245 2120
Fragments arranged by size:
2424 2120 1148 677 323 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2466 513
Size: 552 939 462 4984
Fragments arranged by size:
4984 939 552 462
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6937
NheI G'CTAG_C
Cuts at: 1052 4217 1052
Size: 3165 3772
NotI GC'GGCC_GC
Cuts at: 4384 4384
Size: 6937
NspI r_CATG'y
Cuts at: 1132 1229 2023 2108 2762 4295 5840 1132
Size: 97 794 85 654 1533 1545 2229
Fragments arranged by size:
2229 1545 1533 794 654 97 85
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6937
PciI A'CATG_T
Cuts at: 2758 5836 2758
Size: 3078 3859
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2795 3479 1108
Size: 1687 684 4566
Fragments arranged by size:
4566 1687 684
PmeI GTTT'AAAC
Cuts at: 4241 4241
Size: 6937
PmlI CAC'GTG
Cuts at: 4003 4003
Size: 6937
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5006 5038 6519 6551 5006
Size: 32 1481 32 5392
Fragments arranged by size:
5392 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2118 2118
Size: 6937
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6937
PsiI TTA'TAA
Cuts at: 4507 4507
Size: 6937
PspXI vC'TCGA_Gb
Cuts at: 3549 3549
Size: 6937
PstI C_TGCA'G
Cuts at: 830 2313 4289 830
Size: 1483 1976 3478
Fragments arranged by size:
3478 1976 1483
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6937
PvuII CAG'CTG
Cuts at: 4030 4030
Size: 6937
SalI G'TCGA_C
Cuts at: 1936 4279 1936
Size: 2343 4594
SapI GCTCTTCn'nnn_
Cuts at: 3156 3885 3156
Size: 729 6208
SbfI CC_TGCA'GG
Cuts at: 4289 4289
Size: 6937
ScaI AGT'ACT
Cuts at: 3385 5185 6897 3385
Size: 1800 1712 3425
Fragments arranged by size:
3425 1800 1712
SfcI C'TryA_G
Cuts at: 826 1045 2040 2309 3215 4157 4285 5420 6101
Size: 219 995 269 906 942 128 1135 681
Cuts at: 6101 6292 826
Size: 191 1471
Fragments arranged by size:
1471 1135 995 942 906 681 269 219
191 128
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6937
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6937
SmaI CCC'GGG
Cuts at: 1872 4264 1872
Size: 2392 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3549 4201 5041 5942 6204 820
Size: 197 926 1606 652 840 901 262 1553
Fragments arranged by size:
1606 1553 926 901 840 652 262 197
SnaBI TAC'GTA
Cuts at: 493 4728 493
Size: 4235 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6937
SphI G_CATG'C
Cuts at: 1132 2023 4295 1132
Size: 891 2272 3774
Fragments arranged by size:
3774 2272 891
SspI AAT'ATT
Cuts at: 5 52 4861 5
Size: 47 4809 2081
Fragments arranged by size:
4809 2081 47
StuI AGG'CCT
Cuts at: 1517 2390 1517
Size: 873 6064
StyI C'CwwG_G
Cuts at: 513 1065 2004 2466 2607 4376 513
Size: 552 939 462 141 1769 3074
Fragments arranged by size:
3074 1769 939 552 462 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4964 4981 5134 5319 5764 325
Size: 1255 3384 17 153 185 445 1498
Fragments arranged by size:
3384 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3383 5183 6895
Size: 274 80 33 51 1443 1406 1800 1712
Cuts at: 6895 96
Size: 138
Fragments arranged by size:
1800 1712 1443 1406 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2938 2970 6897 6929 2938
Size: 32 3927 32 2946
Fragments arranged by size:
3927 2946 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6937
XbaI T'CTAG_A
Cuts at: 2671 3556 4273 2671
Size: 885 717 5335
Fragments arranged by size:
5335 885 717
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3388 3388
Size: 6937
XhoI C'TCGA_G
Cuts at: 1943 3549 1943
Size: 1606 5331
XmaI C'CCGG_G
Cuts at: 1870 4262 1870
Size: 2392 4545
XmnI GAAnn'nnTTC
Cuts at: 2367 5066 2367
Size: 2699 4238
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4745 276
Size: 53 83 186 936 3211 2468
Fragments arranged by size:
3211 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PspOMI PsrI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI