Restriction map for
pFN21AB4886
(Circular) (Six-base) MAPSORT of: pFN21AB4886.seq Check: 6138 from: 1 to: 5725
pFN21AB4886 5725 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3535 278
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3068 1937
Size: 1131 4594
Acc65I G'GTAC_C
Cuts at: 2436 3046 2436
Size: 610 5115
AclI AA'CG_TT
Cuts at: 1563 3024 3852 4225 1563
Size: 1461 828 373 3063
Fragments arranged by size:
3063 1461 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3788 5172 5440 1632
Size: 2156 1384 268 1917
Fragments arranged by size:
2156 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5528
AflIII A'CryG_T
Cuts at: 1203 4624 5601 1203
Size: 3421 977 1327
Fragments arranged by size:
3421 1327 977
AgeI A'CCGG_T
Cuts at: 4556 4556
Size: 5725
AhdI GACnn_n'nnGTC
Cuts at: 4454 4454
Size: 5725
AleI CACnn'nnGTG
Cuts at: 1166 2600 1166
Size: 1434 4291
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5725
AlwNI CAG_nnn'CTG
Cuts at: 3468 5040 3468
Size: 1572 4153
ApaLI G'TGCA_C
Cuts at: 3782 4938 3782
Size: 1156 4569
ApoI r'AATT_y
Cuts at: 1493 3034 3260 3412 5521 1493
Size: 1541 226 152 2109 1697
Fragments arranged by size:
2109 1697 1541 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4279 160
Size: 872 3247 1606
Fragments arranged by size:
3247 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
AvaI C'yCGr_G
Cuts at: 1870 1943 2957 3050 1870
Size: 73 1014 93 4545
Fragments arranged by size:
4545 1014 93 73
BamHI G'GATC_C
Cuts at: 3055 3422 3055
Size: 367 5358
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2163 2436 2609 3046 4501
Size: 325 217 627 376 273 173 437 1455
Cuts at: 4501 618
Size: 1842
Fragments arranged by size:
1842 1455 627 437 376 325 273 217
173
BanII G_rGCy'C
Cuts at: 1391 2787 2996 3044 1391
Size: 1396 209 48 4072
Fragments arranged by size:
4072 1396 209 48
BbeI G_GCGC'C
Cuts at: 2613 2613
Size: 5725
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4754
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2675 2709 3916 3950 1903
Size: 34 738 34 1207 34 3678
Fragments arranged by size:
3678 1207 738 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2485 3617 4827 2485
Size: 1132 1210 3383
Fragments arranged by size:
3383 1210 1132
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5725
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4336 136
Size: 107 122 71 631 605 2664 1525
Fragments arranged by size:
2664 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2175 3015 5720 2175
Size: 840 2705 2180
Fragments arranged by size:
2705 2180 840
BlpI GC'TnA_GC
Cuts at: 3142 3142
Size: 5725
Bme1580I G_kGCm'C
Cuts at: 1790 2166 2506 3786 4942 1790
Size: 376 340 1280 1156 2573
Fragments arranged by size:
2573 1280 1156 376 340
BmgBI CAC'GTC
Cuts at: 1318 2641 1318
Size: 1323 4402
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2114 2200 2442
Size: 416 53 83 399 24 697 86 242
Cuts at: 2442 3456 4414 442
Size: 1014 958 1753
Fragments arranged by size:
1753 1014 958 697 416 399 242 86
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2036 4385 1239
Size: 797 2349 2579
Fragments arranged by size:
2579 2349 797
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2188 2366 2996 3850 4715 5013 2188
Size: 178 630 854 865 298 2900
Fragments arranged by size:
2900 865 854 630 298 178
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4388 882
Size: 617 2889 2219
Fragments arranged by size:
2889 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2751 3516 493
Size: 711 1547 765 2702
Fragments arranged by size:
2702 1547 765 711
BsaBI GATnn'nnATC
Cuts at: 5719 5719
Size: 5725
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2610 2745 3532 3914
Size: 53 83 186 936 1077 135 787 382
Cuts at: 3914 4549 275
Size: 635 1451
Fragments arranged by size:
1451 1077 936 787 635 382 186 135
83 53
BsaWI w'CCGG_w
Cuts at: 2046 4158 4556 4830 4977 5388 2046
Size: 2112 398 274 147 411 2383
Fragments arranged by size:
2383 2112 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2351 2381 1178
Size: 30 165 30 948 30 4522
Fragments arranged by size:
4522 948 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2284 2777 1186
Size: 1098 493 4134
Fragments arranged by size:
4134 1098 493
BseYI C'CCAG_C
Cuts at: 2108 4928 5424 2108
Size: 2820 496 2409
Fragments arranged by size:
2820 2409 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2937 1163
Size: 573 1201 3951
Fragments arranged by size:
3951 1201 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2914 3175 3936 4964 1540
Size: 275 186 913 261 761 1028 2301
Fragments arranged by size:
2301 1028 913 761 275 261 186
BsiHKAI G_wGCw'C
Cuts at: 2787 3786 3871 4942 2787
Size: 999 85 1071 3570
Fragments arranged by size:
3570 1071 999 85
BsmI GAATG_Cn'
Cuts at: 3236 3329 3236
Size: 93 5632
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2166 2506 2787 2996 3044 3786 3871
Size: 399 376 340 281 209 48 742 85
Cuts at: 3871 4942 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 376 340 281 209
85 48
BspEI T'CCGG_A
Cuts at: 2046 5388 2046
Size: 3342 2383
BspHI T'CATG_A
Cuts at: 3614 5344 3614
Size: 1730 3995
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2775 3080 844
Size: 1931 305 3489
Fragments arranged by size:
3489 1931 305
BsrBI CCG'CTC
Cuts at: 1394 1823 3612 1394
Size: 429 1789 3507
Fragments arranged by size:
3507 1789 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4220 4394 65
Size: 1183 2972 174 1396
Fragments arranged by size:
2972 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2732 4369 4556 1700
Size: 110 922 1637 187 2869
Fragments arranged by size:
2869 1637 922 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5725
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5725
BssSI C'ACGA_G
Cuts at: 3785 4797 3785
Size: 1012 4713
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5725
BstBI TT'CG_AA
Cuts at: 4529 4529
Size: 5725
BstEII G'GTnAC_C
Cuts at: 1184 2962 1184
Size: 1778 3947
BstYI r'GATC_y
Cuts at: 1972 2055 2175 3015 3055 3422 3807 3824 5276
Size: 83 120 840 40 367 385 17 1452
Cuts at: 5276 5362 5374 5720 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 840 385 367 346 120 86
83 40 17 12
Bsu36I CC'TnA_GG
Cuts at: 2326 2326
Size: 5725
BtgI C'CryG_G
Cuts at: 513 1065 2158 2988 513
Size: 552 1093 830 3250
Fragments arranged by size:
3250 1093 830 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4986
Fragments arranged by size:
4986 683 56
BtsI GCAGTG_nn'
Cuts at: 2703 3241 4035 4055 2703
Size: 538 794 20 4373
Fragments arranged by size:
4373 794 538 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5725
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5690
DraI TTT'AAA
Cuts at: 3029 3029
Size: 5725
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4732 809
Size: 507 3416 1802
Fragments arranged by size:
3416 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2425 2590 2674
Size: 54 1425 50 275 18 595 165 84
Cuts at: 2674 2932 2985 3172 4061 5690 8
Size: 258 53 187 889 1629 43
Fragments arranged by size:
1629 1425 889 595 275 258 187 165
84 54 53 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3172 1537
Size: 275 1360 4090
Fragments arranged by size:
4090 1360 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2765 3655 1353
Size: 1412 890 3423
Fragments arranged by size:
3423 1412 890
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4312 4686 4832 387
Size: 1064 138 2723 374 146 1280
Fragments arranged by size:
2723 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2785 2785
Size: 5725
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 3788 4385 5172 5440 1239
Size: 393 404 1752 597 787 268 1524
Fragments arranged by size:
1752 1524 787 597 404 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2142 2151 2142
Size: 9 5716
EcoRI G'AATT_C
Cuts at: 3034 3412 3034
Size: 378 5347
FspI TGC'GCA
Cuts at: 4231 4231
Size: 5725
HaeII r_GCGC'y
Cuts at: 1678 2223 2613 4872 1678
Size: 545 390 2259 2531
Fragments arranged by size:
2531 2259 545 390
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2552 2584 4366 4398 4440
Size: 32 135 32 980 32 1782 32 42
Cuts at: 4440 4472 1373
Size: 32 2626
Fragments arranged by size:
2626 1782 980 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3069 3315 669
Size: 988 281 1131 246 3079
Fragments arranged by size:
3079 1131 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5725
HpaI GTT'AAC
Cuts at: 3315 3315
Size: 5725
KasI G'GCGC_C
Cuts at: 2609 2609
Size: 5725
KpnI G_GTAC'C
Cuts at: 2440 3050 2440
Size: 610 5115
MfeI C'AATT_G
Cuts at: 3324 3324
Size: 5725
MluI A'CGCG_T
Cuts at: 5601 5601
Size: 5725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2334 2709 4839 5023 1305
Size: 1029 375 2130 184 2007
Fragments arranged by size:
2130 2007 1029 375 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2427 2934 2987 10
Size: 54 1425 343 595 507 53 2748
Fragments arranged by size:
2748 1425 595 507 343 54 53
MslI CAynn'nnrTG
Cuts at: 518 1166 2600 2981 3683 4042 4201 518
Size: 648 1434 381 702 359 159 2042
Fragments arranged by size:
2042 1434 702 648 381 359 159
MspA1I CmG'CkG
Cuts at: 1606 2108 2287 2847 3141 3818 4966 5211 1606
Size: 502 179 560 294 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 560 502 294 245 179
NarI GG'CG_CC
Cuts at: 2610 2610
Size: 5725
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5173
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5725
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5725
NotI GC'GGCC_GC
Cuts at: 3172 3172
Size: 5725
NspI r_CATG'y
Cuts at: 1132 1229 2079 3083 4628 1132
Size: 97 850 1004 1545 2229
Fragments arranged by size:
2229 1545 1004 850 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5725
PciI A'CATG_T
Cuts at: 4624 4624
Size: 5725
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2190 1108
Size: 1082 4643
PfoI T'CCnGG_A
Cuts at: 2058 2457 2058
Size: 399 5326
PmeI GTTT'AAAC
Cuts at: 3029 3029
Size: 5725
PmlI CAC'GTG
Cuts at: 2751 2751
Size: 5725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3794 3826 5307 5339 3794
Size: 32 1481 32 4180
Fragments arranged by size:
4180 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2142 2151 2142
Size: 9 5716
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5725
PsiI TTA'TAA
Cuts at: 3295 3295
Size: 5725
PstI C_TGCA'G
Cuts at: 830 3077 830
Size: 2247 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5725
PvuII CAG'CTG
Cuts at: 2108 2108
Size: 5725
SacI G_AGCT'C
Cuts at: 2787 2787
Size: 5725
SalI G'TCGA_C
Cuts at: 1936 3067 1936
Size: 1131 4594
SbfI CC_TGCA'GG
Cuts at: 3077 3077
Size: 5725
ScaI AGT'ACT
Cuts at: 3973 5685 3973
Size: 1712 4013
SfcI C'TryA_G
Cuts at: 826 1045 2374 3073 4208 4889 5080 826
Size: 219 1329 699 1135 681 191 1471
Fragments arranged by size:
1471 1329 1135 699 681 219 191
SfoI GGC'GCC
Cuts at: 2611 2611
Size: 5725
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5725
SmaI CCC'GGG
Cuts at: 1872 3052 1872
Size: 1180 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2203 2381 3011 3829 4730 4992
Size: 197 926 260 178 630 818 901 262
Cuts at: 4992 820
Size: 1553
Fragments arranged by size:
1553 926 901 818 630 262 260 197
178
SnaBI TAC'GTA
Cuts at: 493 3516 493
Size: 3023 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5725
SphI G_CATG'C
Cuts at: 1132 3083 1132
Size: 1951 3774
SspI AAT'ATT
Cuts at: 5 52 3649 5
Size: 47 3597 2081
Fragments arranged by size:
3597 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5725
StyI C'CwwG_G
Cuts at: 513 1065 3164 513
Size: 552 2099 3074
Fragments arranged by size:
3074 2099 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2213 3752 3769 3922 4107 4552 325
Size: 1255 633 1539 17 153 185 445 1498
Fragments arranged by size:
1539 1498 1255 633 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2793 3971 5683
Size: 274 80 33 51 1443 816 1178 1712
Cuts at: 5683 96
Size: 138
Fragments arranged by size:
1712 1443 1178 816 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2143 2175 2639 2671 5685 5717 2143
Size: 32 464 32 3014 32 2151
Fragments arranged by size:
3014 2151 464 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5725
XbaI T'CTAG_A
Cuts at: 3061 3061
Size: 5725
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2105 2011
Size: 94 5631
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5725
XmaI C'CCGG_G
Cuts at: 1870 3050 1870
Size: 1180 4545
XmnI GAAnn'nnTTC
Cuts at: 3854 3854
Size: 5725
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3533 276
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SrfI SwaI