Restriction map for
pFN21AB4872
(Circular) (Six-base) MAPSORT of: pFN21AB4872.seq Check: 9757 from: 1 to: 13537
pFN21AB4872 13537 bp
With 182 enzymes: SgfI *
August 20, 2012 11:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6547 11347 278
Size: 53 83 186 936 5011 4800 2468
Fragments arranged by size:
5011 4800 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4250 7131 8464 10880 1937
Size: 2313 2881 1333 2416 4594
Fragments arranged by size:
4594 2881 2416 2313 1333
Acc65I G'GTAC_C
Cuts at: 10537 10680 10858 10537
Size: 143 178 13216
Fragments arranged by size:
13216 178 143
AclI AA'CG_TT
Cuts at: 1563 11664 12037 1563
Size: 10101 373 3063
Fragments arranged by size:
10101 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2513 2573 2590 6585 8036 8514 8639 8691
Size: 881 60 17 3995 1451 478 125 52
Cuts at: 8691 10202 10623 11600 12984 13252 1632
Size: 1511 421 977 1384 268 1917
Fragments arranged by size:
3995 1917 1511 1451 1384 977 881 478
421 268 125 60 52 17
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 13340
AflIII A'CryG_T
Cuts at: 1203 4891 5436 12436 13413 1203
Size: 3688 545 7000 977 1327
Fragments arranged by size:
7000 3688 1327 977 545
AgeI A'CCGG_T
Cuts at: 3336 5843 12368 3336
Size: 2507 6525 4505
Fragments arranged by size:
6525 4505 2507
AhdI GACnn_n'nnGTC
Cuts at: 2420 2455 12266 2420
Size: 35 9811 3691
Fragments arranged by size:
9811 3691 35
AleI CACnn'nnGTG
Cuts at: 1166 5414 1166
Size: 4248 9289
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3641 5372 1184
Size: 2457 1731 9349
Fragments arranged by size:
9349 2457 1731
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2991 3023 5156 5188 7443 7475 2991
Size: 32 2133 32 2255 32 9053
Fragments arranged by size:
9053 2255 2133 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2366 2467 2830 3121 3265 3599 3931 4244 4487
Size: 101 363 291 144 334 332 313 243
Cuts at: 4487 6881 7142 8306 10322 10472 10826 11280 12852
Size: 2394 261 1164 2016 150 354 454 1572
Cuts at: 12852 2366
Size: 3051
Fragments arranged by size:
3051 2394 2016 1572 1164 454 363 354
334 332 313 291 261 243 150 144
101
ApaI G_GGCC'C
Cuts at: 3105 3128 3219 3287 4028 4067 4270 4293 4746
Size: 23 91 68 741 39 203 23 453
Cuts at: 4746 4836 5868 6128 6410 6465 6687 6696 6731
Size: 90 1032 260 282 55 222 9 35
Cuts at: 6731 7200 7380 7694 7736 7970 7988 8081 8262
Size: 469 180 314 42 234 18 93 181
Cuts at: 8262 8896 9414 9495 10021 3105
Size: 634 518 81 526 6621
Fragments arranged by size:
6621 1032 741 634 526 518 469 453
314 282 260 234 222 203 181 180
93 91 90 81 68 55 42 39
35 23 23 18 9
ApaLI G'TGCA_C
Cuts at: 2223 2650 11594 12750 2223
Size: 427 8944 1156 3010
Fragments arranged by size:
8944 3010 1156 427
ApoI r'AATT_y
Cuts at: 1493 10846 11072 11224 13333 1493
Size: 9353 226 152 2109 1697
Fragments arranged by size:
9353 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 12091 160
Size: 872 11059 1606
Fragments arranged by size:
11059 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13537
AvaI C'yCGr_G
Cuts at: 1870 1943 2061 3563 3735 4731 4745 5833 6013
Size: 73 118 1502 172 996 14 1088 180
Cuts at: 6013 6030 6519 6891 6946 7117 7191 8084 8129
Size: 17 489 372 55 171 74 893 45
Cuts at: 8129 8458 8575 9130 9220 9237 9414 9477 9633
Size: 329 117 555 90 17 177 63 156
Cuts at: 9633 9660 9796 9867 9959 10332 10797 10862 1870
Size: 27 136 71 92 373 465 65 4545
Fragments arranged by size:
4545 1502 1088 996 893 555 489 465
373 372 329 180 177 172 171 156
136 118 117 92 90 74 73 71
65 63 55 45 27 17 17 14
AvrII C'CTAG_G
Cuts at: 4813 4813
Size: 13537
BamHI G'GATC_C
Cuts at: 2043 5364 6515 7760 9783 9800 10867 11234 2043
Size: 3321 1151 1245 2023 17 1067 367 4346
Fragments arranged by size:
4346 3321 2023 1245 1151 1067 367 17
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2056 3059 3316 3602 4136
Size: 325 217 627 269 1003 257 286 534
Cuts at: 4136 4458 4672 5443 5460 5614 6988 7592 8570
Size: 322 214 771 17 154 1374 604 978
Cuts at: 8570 8792 9602 10537 10680 10858 12313 618
Size: 222 810 935 143 178 1455 1842
Fragments arranged by size:
1842 1455 1374 1003 978 935 810 771
627 604 534 325 322 286 269 257
222 217 214 178 154 143 17
BanII G_rGCy'C
Cuts at: 1391 2074 3105 3128 3219 3287 3533 3593 4028
Size: 683 1031 23 91 68 246 60 435
Cuts at: 4028 4067 4270 4293 4746 4752 4836 5742 5868
Size: 39 203 23 453 6 84 906 126
Cuts at: 5868 5968 5984 6058 6128 6367 6410 6465 6687
Size: 100 16 74 70 239 43 55 222
Cuts at: 6687 6696 6731 7200 7228 7380 7642 7694 7736
Size: 9 35 469 28 152 262 52 42
Cuts at: 7736 7904 7970 7988 8081 8136 8262 8779 8825
Size: 168 66 18 93 55 126 517 46
Cuts at: 8825 8896 8919 8947 9054 9088 9137 9414 9495
Size: 71 23 28 107 34 49 277 81
Cuts at: 9495 9722 10021 10270 10288 10655 10856 1391
Size: 227 299 249 18 367 201 4072
Fragments arranged by size:
4072 1031 906 683 517 469 453 435
367 299 277 262 249 246 239 227
222 203 201 168 152 126 126 107
100 93 91 84 81 74 71 70
68 66 60 55 55 52 49 46
43 42 39 35 34 28 28 23
23 23 18 18 16 9 6
BbeI G_GCGC'C
Cuts at: 2060 4140 5464 5618 7596 2060
Size: 2080 1324 154 1978 8001
Fragments arranged by size:
8001 2080 1978 1324 154
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2386 6278 7566 928
Size: 971 487 3892 1288 6899
Fragments arranged by size:
6899 3892 1288 971 487
BbvCI CC'TCA_GC
Cuts at: 4652 4652
Size: 13537
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2167 2201 5588 5622 11728 11762 1903
Size: 34 230 34 3387 34 6106 34 3678
Fragments arranged by size:
6106 3678 3387 230 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4139 7658 9839 11429 12639 4139
Size: 3519 2181 1590 1210 5037
Fragments arranged by size:
5037 3519 2181 1590 1210
BclI T'GATC_A
Cuts at: 1548 2442 1548
Size: 894 12643
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4214 4274 5154
Size: 107 122 71 631 605 2542 60 880
Cuts at: 5154 5263 5835 5979 6318 6691 6735 6804 7384
Size: 109 572 144 339 373 44 69 580
Cuts at: 7384 7464 7698 7930 7974 7992 8532 8709 9186
Size: 80 234 232 44 18 540 177 477
Cuts at: 9186 9462 12148 136
Size: 276 2686 1525
Fragments arranged by size:
2686 2542 1525 880 631 605 580 572
540 477 373 339 276 234 232 177
144 122 109 107 80 71 69 60
44 44 18
BglII A'GATC_T
Cuts at: 9172 13532 9172
Size: 4360 9177
BlpI GC'TnA_GC
Cuts at: 2333 3710 4218 4751 9721 10954 2333
Size: 1377 508 533 4970 1233 4916
Fragments arranged by size:
4970 4916 1377 1233 533 508
Bme1580I G_kGCm'C
Cuts at: 1790 2227 2654 3105 3128 3219 3287 3437 4028
Size: 437 427 451 23 91 68 150 591
Cuts at: 4028 4067 4270 4293 4565 4677 4746 4836 5150
Size: 39 203 23 272 112 69 90 314
Cuts at: 5150 5411 5868 6128 6410 6465 6687 6696 6731
Size: 261 457 260 282 55 222 9 35
Cuts at: 6731 6991 7200 7380 7694 7736 7926 7970 7988
Size: 260 209 180 314 42 190 44 18
Cuts at: 7988 8081 8262 8575 8896 9414 9495 10021 11598
Size: 93 181 313 321 518 81 526 1577
Cuts at: 11598 12754 1790
Size: 1156 2573
Fragments arranged by size:
2573 1577 1156 591 526 518 457 451
437 427 321 314 314 313 282 272
261 260 260 222 209 203 190 181
180 150 112 93 91 90 81 69
68 55 44 42 39 35 23 23
18 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 13537
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3587 3604 4061
Size: 416 53 83 399 24 2170 17 457
Cuts at: 4061 4070 4183 5741 6158 8531 10140 11268 12226
Size: 9 113 1558 417 2373 1609 1128 958
Cuts at: 12226 442
Size: 1753
Fragments arranged by size:
2373 2170 1753 1609 1558 1128 958 457
417 416 399 113 83 53 24 17
9
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 13537
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2232 3488 3607 3891 3916 4253 4425 4796
Size: 993 1256 119 284 25 337 172 371
Cuts at: 4796 4845 4920 5252 5604 5802 5928 5970 5982
Size: 49 75 332 352 198 126 42 12
Cuts at: 5982 6351 6444 6498 6570 6771 6798 6861 7014
Size: 369 93 54 72 201 27 63 153
Cuts at: 7014 7059 7164 7242 7575 7653 7806 8022 8337
Size: 45 105 78 333 78 153 216 315
Cuts at: 8337 8631 8652 8694 8718 8943 8970 8982 9072
Size: 294 21 42 24 225 27 12 90
Cuts at: 9072 9141 9414 9573 10302 12197 1239
Size: 69 273 159 729 1895 2579
Fragments arranged by size:
2579 1895 1256 993 729 371 369 352
337 333 332 315 294 284 273 225
216 201 198 172 159 153 153 126
119 105 93 90 78 78 75 72
69 63 54 49 45 42 42 27
27 25 24 21 12 12
Bpu10I CC'TnA_GC
Cuts at: 4323 4652 8349 4323
Size: 329 3697 9511
Fragments arranged by size:
9511 3697 329
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2635 2767 5017 5175 5530 8324 9860 11662 12527
Size: 132 2250 158 355 2794 1536 1802 865
Cuts at: 12527 12825 2635
Size: 298 3347
Fragments arranged by size:
3347 2794 2250 1802 1536 865 355 298
158 132
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2706 2864 2897 4508 6396 6747 6765
Size: 617 1207 158 33 1611 1888 351 18
Cuts at: 6765 6774 6846 7017 7880 8025 8412 8547 8736
Size: 9 72 171 863 145 387 135 189
Cuts at: 8736 8958 9051 9381 12200 882
Size: 222 93 330 2819 2219
Fragments arranged by size:
2819 2219 1888 1611 1207 863 617 387
351 330 222 189 171 158 145 135
93 72 33 18 9
BsaAI yAC'GTr
Cuts at: 493 1204 4627 4892 5473 11328 493
Size: 711 3423 265 581 5855 2702
Fragments arranged by size:
5855 3423 2702 711 581 265
BsaBI GATnn'nnATC
Cuts at: 13531 13531
Size: 13537
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2057 4137 5067 5090
Size: 53 83 186 936 524 2080 930 23
Cuts at: 5090 5138 5461 5615 5920 6544 7593 7933 11344
Size: 48 323 154 305 624 1049 340 3411
Cuts at: 11344 11726 12361 275
Size: 382 635 1451
Fragments arranged by size:
3411 2080 1451 1049 936 930 635 624
524 382 340 323 305 186 154 83
53 48 23
BsaWI w'CCGG_w
Cuts at: 2686 3336 3913 5843 6707 11970 12368 12642 12789
Size: 650 577 1930 864 5263 398 274 147
Cuts at: 12789 13200 2686
Size: 411 3023
Fragments arranged by size:
5263 3023 1930 864 650 577 411 398
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2668 2689 2698 2719 5066
Size: 30 165 30 1265 21 9 21 2347
Cuts at: 5066 5096 5833 5863 7443 7473 7594 7624 8629
Size: 30 737 30 1580 30 121 30 1005
Cuts at: 8629 8659 8812 8842 8955 8985 9111 9141 10242
Size: 30 153 30 113 30 126 30 1101
Cuts at: 10242 10272 10434 10464 1178
Size: 30 162 30 4251
Fragments arranged by size:
4251 2347 1580 1265 1101 1005 737 165
162 153 126 121 113 30 30 30
30 30 30 30 30 30 30 30
30 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2131 2561 2796 4034 5236 6714 7818 9255
Size: 945 430 235 1238 1202 1478 1104 1437
Cuts at: 9255 9849 10538 10601 1186
Size: 594 689 63 4122
Fragments arranged by size:
4122 1478 1437 1238 1202 1104 945 689
594 430 235 63
BseYI C'CCAG_C
Cuts at: 2073 2403 2818 2860 3121 3704 4662 5040 6508
Size: 330 415 42 261 583 958 378 1468
Cuts at: 6508 6689 7297 7373 7409 8147 8486 10482 12740
Size: 181 608 76 36 738 339 1996 2258
Cuts at: 12740 13236 2073
Size: 496 2374
Fragments arranged by size:
2374 2258 1996 1468 958 738 608 583
496 415 378 339 330 261 181 76
42 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2080 2270 4524 4652 4682 9063 9561
Size: 573 344 190 2254 128 30 4381 498
Cuts at: 9561 9795 10338 10642 1163
Size: 234 543 304 4058
Fragments arranged by size:
4381 4058 2254 573 543 498 344 304
234 190 128 30
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4911 6217 8026 10987 11748 12776
Size: 275 186 2910 1306 1809 2961 761 1028
Cuts at: 12776 1540
Size: 2301
Fragments arranged by size:
2961 2910 2301 1809 1306 1028 761 275
186
BsiHKAI G_wGCw'C
Cuts at: 2227 2654 3194 3902 4647 4752 5851 6367 7642
Size: 427 540 708 745 105 1099 516 1275
Cuts at: 7642 7904 9202 10288 10506 11598 11683 12754 2227
Size: 262 1298 1086 218 1092 85 1071 3010
Fragments arranged by size:
3010 1298 1275 1099 1092 1086 1071 745
708 540 516 427 262 218 105 85
BsmI GAATG_Cn'
Cuts at: 2539 3680 10753 11048 11141 2539
Size: 1141 7073 295 93 4935
Fragments arranged by size:
7073 4935 1141 295 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5074 7419 10516 5074
Size: 2345 3097 8095
Fragments arranged by size:
8095 3097 2345
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2074 2227 2654 3105 3128 3194 3219
Size: 399 284 153 427 451 23 66 25
Cuts at: 3219 3287 3437 3533 3593 3902 4028 4067 4270
Size: 68 150 96 60 309 126 39 203
Cuts at: 4270 4293 4565 4647 4677 4746 4752 4836 5150
Size: 23 272 82 30 69 6 84 314
Cuts at: 5150 5411 5742 5851 5868 5968 5984 6058 6128
Size: 261 331 109 17 100 16 74 70
Cuts at: 6128 6367 6410 6465 6687 6696 6731 6991 7200
Size: 239 43 55 222 9 35 260 209
Cuts at: 7200 7228 7380 7642 7694 7736 7904 7926 7970
Size: 28 152 262 52 42 168 22 44
Cuts at: 7970 7988 8081 8136 8262 8575 8779 8825 8896
Size: 18 93 55 126 313 204 46 71
Cuts at: 8896 8919 8947 9054 9088 9137 9202 9414 9495
Size: 23 28 107 34 49 65 212 81
Cuts at: 9495 9722 10021 10270 10288 10506 10655 10856 11598
Size: 227 299 249 18 218 149 201 742
Cuts at: 11598 11683 12754 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 451 427 399 331 314
313 309 299 284 272 262 261 260
249 239 227 222 218 212 209 204
203 201 168 153 152 150 149 126
126 109 107 100 96 93 85 84
82 81 74 71 70 69 68 66
65 60 55 55 52 49 46 44
43 42 39 35 34 30 28 28
25 23 23 23 22 18 18 17
16 9 6
BspEI T'CCGG_A
Cuts at: 2686 3913 13200 2686
Size: 1227 9287 3023
Fragments arranged by size:
9287 3023 1227
BspHI T'CATG_A
Cuts at: 11426 13156 11426
Size: 1730 11807
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2102 3552 6652 10892 844
Size: 1258 1450 3100 4240 3489
Fragments arranged by size:
4240 3489 3100 1450 1258
BsrBI CCG'CTC
Cuts at: 1394 1823 2842 2964 3649 5588 6226 7347 9274
Size: 429 1019 122 685 1939 638 1121 1927
Cuts at: 9274 10111 10534 11424 1394
Size: 837 423 890 3507
Fragments arranged by size:
3507 1939 1927 1121 1019 890 837 685
638 429 423 122
BsrDI GCAATG_nn'
Cuts at: 65 1248 6255 8374 12032 12206 65
Size: 1183 5007 2119 3658 174 1396
Fragments arranged by size:
5007 3658 2119 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3336 4669 5843 10325 10475 12181 12368
Size: 110 1526 1333 1174 4482 150 1706 187
Cuts at: 12368 1700
Size: 2869
Fragments arranged by size:
4482 2869 1706 1526 1333 1174 187 150
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 13537
BssHII G'CGCG_C
Cuts at: 1925 4853 1925
Size: 2928 10609
BssSI C'ACGA_G
Cuts at: 2709 3476 4869 6003 6138 11597 12609 2709
Size: 767 1393 1134 135 5459 1012 3637
Fragments arranged by size:
5459 3637 1393 1134 1012 767 135
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2222 3265 3574 5450 10472 794
Size: 1428 1043 309 1876 5022 3859
Fragments arranged by size:
5022 3859 1876 1428 1043 309
BstBI TT'CG_AA
Cuts at: 12341 12341
Size: 13537
BstEII G'GTnAC_C
Cuts at: 1184 3181 6443 6941 7085 7313 7742 8372 8838
Size: 1997 3262 498 144 228 429 630 466
Cuts at: 8838 8993 9110 9791 10052 10088 1184
Size: 155 117 681 261 36 4633
Fragments arranged by size:
4633 3262 1997 681 630 498 466 429
261 228 155 144 117 36
BstXI CCAn_nnnn'nTGG
Cuts at: 3419 4277 5345 5552 3419
Size: 858 1068 207 11404
Fragments arranged by size:
11404 1068 858 207
BstYI r'GATC_y
Cuts at: 1972 2043 4353 4494 5028 5364 6515 7033 7760
Size: 71 2310 141 534 336 1151 518 727
Cuts at: 7760 9172 9783 9800 10867 11234 11619 11636 13088
Size: 1412 611 17 1067 367 385 17 1452
Cuts at: 13088 13174 13186 13532 1972
Size: 86 12 346 1977
Fragments arranged by size:
2310 1977 1452 1412 1151 1067 727 611
534 518 385 367 346 336 141 86
71 17 17 12
BstZ17I GTA'TAC
Cuts at: 4251 4251
Size: 13537
Bsu36I CC'TnA_GG
Cuts at: 2544 3321 4596 4710 5118 8043 10545 2544
Size: 777 1275 114 408 2925 2502 5536
Fragments arranged by size:
5536 2925 2502 1275 777 408 114
BtgI C'CryG_G
Cuts at: 513 1065 2267 3328 3498 4171 4209 5152 5415
Size: 552 1202 1061 170 673 38 943 263
Cuts at: 5415 5649 6166 6410 7090 7174 7444 7747 8026
Size: 234 517 244 680 84 270 303 279
Cuts at: 8026 8886 9596 513
Size: 860 710 4454
Fragments arranged by size:
4454 1202 1061 943 860 710 680 673
552 517 303 279 270 263 244 234
170 84 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2274 10414 487
Size: 683 56 1048 8140 3610
Fragments arranged by size:
8140 3610 1048 683 56
BtsI GCAGTG_nn'
Cuts at: 2828 2979 3214 3763 3913 3929 5880 6639 10195
Size: 151 235 549 150 16 1951 759 3556
Cuts at: 10195 10470 10659 11053 11847 11867 2828
Size: 275 189 394 794 20 4498
Fragments arranged by size:
4498 3556 1951 794 759 549 394 275
235 189 151 150 20 16
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 13537
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 6720 6755 529
Size: 35 6156 35 7311
Fragments arranged by size:
7311 6156 35 35
DraI TTT'AAA
Cuts at: 10841 10841
Size: 13537
DraIII CAC_nnn'GTG
Cuts at: 2231 3481 4630 4871 8570 9602 2231
Size: 1250 1149 241 3699 1032 6166
Fragments arranged by size:
6166 3699 1250 1149 1032 241
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4939 5678 7185 7286 8442 9119 9762
Size: 507 3623 739 1507 101 1156 677 643
Cuts at: 9762 12544 809
Size: 2782 1802
Fragments arranged by size:
3623 2782 1802 1507 1156 739 677 643
507 101
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2152 2766
Size: 54 1425 50 275 18 195 127 614
Cuts at: 2766 2897 3202 3483 4717 4973 4990 5691 5732
Size: 131 305 281 1234 256 17 701 41
Cuts at: 5732 5935 6586 7339 7699 9037 9454 9463 10313
Size: 203 651 753 360 1338 417 9 850
Cuts at: 10313 10984 11873 13502 8
Size: 671 889 1629 43
Fragments arranged by size:
1629 1425 1338 1234 889 850 753 701
671 651 614 417 360 305 281 275
256 203 195 131 127 54 50 43
41 18 17 9
EagI C'GGCC_G
Cuts at: 1537 1812 10984 1537
Size: 275 9172 4090
Fragments arranged by size:
9172 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 10524 11467 1353
Size: 9171 943 3423
Fragments arranged by size:
9171 3423 943
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3061 3767 4593 5177 6115 12124
Size: 1064 138 1472 706 826 584 938 6009
Cuts at: 12124 12498 12644 387
Size: 374 146 1280
Fragments arranged by size:
6009 1472 1280 1064 938 826 706 584
374 146 138
EcoICRI GAG'CTC
Cuts at: 4750 6365 7640 7902 10286 4750
Size: 1615 1275 262 2384 8001
Fragments arranged by size:
8001 2384 1615 1275 262
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2232 2513 2573 2590 3488 3607 3891
Size: 393 600 281 60 17 898 119 284
Cuts at: 3891 3916 4253 4425 4796 4845 4920 5252 5604
Size: 25 337 172 371 49 75 332 352
Cuts at: 5604 5802 5928 5970 5982 6351 6444 6498 6570
Size: 198 126 42 12 369 93 54 72
Cuts at: 6570 6585 6771 6798 6861 7014 7059 7164 7242
Size: 15 186 27 63 153 45 105 78
Cuts at: 7242 7575 7653 7806 8022 8036 8337 8514 8631
Size: 333 78 153 216 14 301 177 117
Cuts at: 8631 8639 8652 8691 8694 8718 8943 8970 8982
Size: 8 13 39 3 24 225 27 12
Cuts at: 8982 9072 9141 9414 9573 10202 10302 10623 11600
Size: 90 69 273 159 629 100 321 977
Cuts at: 11600 12197 12984 13252 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 977 898 787 629 600 597 393
371 369 352 337 333 332 321 301
284 281 273 268 225 216 198 186
177 172 159 153 153 126 119 117
105 100 93 90 78 78 75 72
69 63 60 54 49 45 42 39
27 27 25 24 17 15 14 13
12 12 8 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2051 4123 5672 5705 6240 6470 9396 2051
Size: 2072 1549 33 535 230 2926 6192
Fragments arranged by size:
6192 2926 2072 1549 535 230 33
EcoO109I rG'GnC_Cy
Cuts at: 2464 3088 3125 3215 3284 3743 3860 4024 4025
Size: 624 37 90 69 459 117 164 1
Cuts at: 4025 4266 4290 4735 4742 4832 4833 4985 5007
Size: 241 24 445 7 90 1 152 22
Cuts at: 5007 5114 5227 5864 5865 5955 6010 6026 6083
Size: 107 113 637 1 90 55 16 57
Cuts at: 6083 6109 6125 6154 6161 6230 6311 6406 6416
Size: 26 16 29 7 69 81 95 10
Cuts at: 6416 6461 6462 6560 6578 6625 6641 6683 6727
Size: 45 1 98 18 47 16 42 44
Cuts at: 6727 6788 6815 6833 6878 6932 7004 7103 7112
Size: 61 27 18 45 54 72 99 9
Cuts at: 7112 7139 7187 7196 7259 7279 7322 7377 7414
Size: 27 48 9 63 20 43 55 37
Cuts at: 7414 7456 7549 7664 7691 7733 7777 7940 7966
Size: 42 93 115 27 42 44 163 26
Cuts at: 7966 7967 7984 7985 8077 8078 8259 8303 8327
Size: 1 17 1 92 1 181 44 24
Cuts at: 8327 8381 8525 8597 8606 8642 8656 8892 8893
Size: 54 144 72 9 36 14 236 1
Cuts at: 8893 9092 9127 9233 9260 9287 9314 9360 9411
Size: 199 35 106 27 27 27 46 51
Cuts at: 9411 9473 9491 9492 9584 9629 9656 9676 9755
Size: 62 18 1 92 45 27 20 79
Cuts at: 9755 9863 9871 10017 10018 10033 10079 10142 10205
Size: 108 8 146 1 15 46 63 63
Cuts at: 10205 10241 10276 10319 10597 10764 2464
Size: 36 35 43 278 167 5237
Fragments arranged by size:
5237 637 624 459 445 278 241 236
199 181 167 164 163 152 146 144
117 115 113 108 107 106 99 98
95 93 92 92 90 90 90 81
79 72 72 69 69 63 63 63
62 61 57 55 55 54 54 51
48 47 46 46 45 45 45 44
44 44 43 43 42 42 42 37
37 36 36 35 35 29 27 27
27 27 27 27 27 26 26 24
24 22 20 20 18 18 18 17
16 16 16 15 14 10 9 9
9 8 7 7 1 1 1 1
1 1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 10846 11224 10846
Size: 378 13159
FspI TGC'GCA
Cuts at: 3629 12043 3629
Size: 8414 5123
HaeII r_GCGC'y
Cuts at: 1678 2060 4140 5464 5618 7596 10786 12684 1678
Size: 382 2080 1324 154 1978 3190 1898 2531
Fragments arranged by size:
3190 2531 2080 1978 1898 1324 382 154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2126 2158 2406 2438 2441
Size: 32 135 32 554 32 248 32 3
Cuts at: 2441 2473 2668 2684 2689 2700 2716 2721 4486
Size: 32 195 16 5 11 16 5 1765
Cuts at: 4486 4518 5833 5865 6054 6086 7594 7626 7745
Size: 32 1315 32 189 32 1508 32 119
Cuts at: 7745 7777 7886 7918 8629 8661 9111 9143 9220
Size: 32 109 32 711 32 450 32 77
Cuts at: 9220 9252 9291 9323 9704 9736 9785 9817 10544
Size: 32 39 32 381 32 49 32 727
Cuts at: 10544 10576 10637 10669 12178 12210 12252 12284 1373
Size: 32 61 32 1509 32 42 32 2626
Fragments arranged by size:
2626 1765 1509 1508 1315 727 711 554
450 381 248 195 189 135 119 109
77 61 49 42 39 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 16 16 11 5 5 3
HincII GTy'rAC
Cuts at: 669 1657 1938 9767 10881 11127 669
Size: 988 281 7829 1114 246 3079
Fragments arranged by size:
7829 3079 1114 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 13537
HpaI GTT'AAC
Cuts at: 11127 11127
Size: 13537
KasI G'GCGC_C
Cuts at: 2056 4136 5460 5614 7592 2056
Size: 2080 1324 154 1978 8001
Fragments arranged by size:
8001 2080 1978 1324 154
KpnI G_GTAC'C
Cuts at: 10541 10684 10862 10541
Size: 143 178 13216
Fragments arranged by size:
13216 178 143
MfeI C'AATT_G
Cuts at: 11136 11136
Size: 13537
MluI A'CGCG_T
Cuts at: 13413 13413
Size: 13537
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3321 3396 6892 8842 12651 12835 1305
Size: 2016 75 3496 1950 3809 184 2007
Fragments arranged by size:
3809 3496 2016 2007 1950 184 75
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3485 4719 4992 5693 7701
Size: 54 1425 343 1653 1234 273 701 2008
Cuts at: 7701 10
Size: 5846
Fragments arranged by size:
5846 2008 1653 1425 1234 701 343 273
54
MslI CAynn'nnrTG
Cuts at: 518 1166 3623 5414 11495 11854 12013 518
Size: 648 2457 1791 6081 359 159 2042
Fragments arranged by size:
6081 2457 2042 1791 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2114 2407 2480 2765 2864 3571 4823 5044
Size: 508 293 73 285 99 707 1252 221
Cuts at: 5044 5602 6512 6689 7176 7373 9572 10788 10953
Size: 558 910 177 487 197 2199 1216 165
Cuts at: 10953 11630 12778 13023 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2199 2120 1252 1216 1148 910 707 677
558 508 487 293 285 245 221 197
177 165 99 73
NaeI GCC'GGC
Cuts at: 10477 10477
Size: 13537
NarI GG'CG_CC
Cuts at: 2057 4137 5461 5615 7593 2057
Size: 2080 1324 154 1978 8001
Fragments arranged by size:
8001 2080 1978 1324 154
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 12985
NdeI CA'TA_TG
Cuts at: 387 387
Size: 13537
NgoMIV G'CCGG_C
Cuts at: 10475 10475
Size: 13537
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 13537
NotI GC'GGCC_GC
Cuts at: 10984 10984
Size: 13537
NspI r_CATG'y
Cuts at: 1132 1229 4262 5186 5377 5440 10895 12440 1132
Size: 97 3033 924 191 63 5455 1545 2229
Fragments arranged by size:
5455 3033 2229 1545 924 191 97 63
PasI CC'CwG_GG
Cuts at: 1348 3128 3637 4612 5860 6176 6466 6620 6857
Size: 1780 509 975 1248 316 290 154 237
Cuts at: 6857 7229 7328 7418 7660 7679 8255 9062 9140
Size: 372 99 90 242 19 576 807 78
Cuts at: 9140 9293 9319 9760 10175 1348
Size: 153 26 441 415 4710
Fragments arranged by size:
4710 1780 1248 975 807 576 509 441
415 372 316 290 242 237 154 153
99 90 78 26 19
PciI A'CATG_T
Cuts at: 5436 12436 5436
Size: 7000 6537
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2467 3481 3599 4119 5330 8306 8543 10127
Size: 1359 1014 118 520 1211 2976 237 1584
Cuts at: 10127 1108
Size: 4518
Fragments arranged by size:
4518 2976 1584 1359 1211 1014 520 237
118
PfoI T'CCnGG_A
Cuts at: 2639 3305 3584 5097 5229 6058 6184 6289 6421
Size: 666 279 1513 132 829 126 105 132
Cuts at: 6421 6547 6748 6838 7036 7999 8236 8305 8647
Size: 126 201 90 198 963 237 69 342
Cuts at: 8647 8689 9088 2639
Size: 42 399 7088
Fragments arranged by size:
7088 1513 963 829 666 399 342 279
237 201 198 132 132 126 126 105
90 69 42
PmeI GTTT'AAAC
Cuts at: 10841 10841
Size: 13537
PmlI CAC'GTG
Cuts at: 4627 4627
Size: 13537
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7443 7475 11606 11638 13119 13151 7443
Size: 32 4131 32 1481 32 7829
Fragments arranged by size:
7829 4131 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2464 3088 3743 3860 4735 5007 5114 5227 5955
Size: 624 655 117 875 272 107 113 728
Cuts at: 5955 6010 6026 6083 6109 6154 6161 6230 6416
Size: 55 16 57 26 45 7 69 186
Cuts at: 6416 6560 6578 6625 6641 6788 6815 6878 6932
Size: 144 18 47 16 147 27 63 54
Cuts at: 6932 7103 7112 7139 7187 7259 7279 7322 7414
Size: 171 9 27 48 72 20 43 92
Cuts at: 7414 7549 8303 8327 8381 8597 8606 8642 9092
Size: 135 754 24 54 216 9 36 450
Cuts at: 9092 9127 9314 9473 9656 9676 9755 9863 10241
Size: 35 187 159 183 20 79 108 378
Cuts at: 10241 10276 10597 10764 2464
Size: 35 321 167 5237
Fragments arranged by size:
5237 875 754 728 655 624 450 378
321 272 216 187 186 183 171 167
159 147 144 135 117 113 108 107
92 79 72 69 63 57 55 54
54 48 47 45 43 36 35 35
27 27 26 24 20 20 18 16
16 9 9 7
PshAI GACnn'nnGTC
Cuts at: 1613 5093 6786 6930 7320 8379 1613
Size: 3480 1693 144 390 1059 6771
Fragments arranged by size:
6771 3480 1693 1059 390 144
PsiI TTA'TAA
Cuts at: 11107 11107
Size: 13537
PspOMI G'GGCC_C
Cuts at: 3101 3124 3215 3283 4024 4063 4266 4289 4742
Size: 23 91 68 741 39 203 23 453
Cuts at: 4742 4832 5864 6124 6406 6461 6683 6692 6727
Size: 90 1032 260 282 55 222 9 35
Cuts at: 6727 7196 7376 7690 7732 7966 7984 8077 8258
Size: 469 180 314 42 234 18 93 181
Cuts at: 8258 8892 9410 9491 10017 3101
Size: 634 518 81 526 6621
Fragments arranged by size:
6621 1032 741 634 526 518 469 453
314 282 260 234 222 203 181 180
93 91 90 81 68 55 42 39
35 23 23 18 9
PspXI vC'TCGA_Gb
Cuts at: 3735 6030 7191 9237 10332 3735
Size: 2295 1161 2046 1095 6940
Fragments arranged by size:
6940 2295 2046 1161 1095
PstI C_TGCA'G
Cuts at: 830 3429 3972 4036 4353 4492 4843 6634 7402
Size: 2599 543 64 317 139 351 1791 768
Cuts at: 7402 8514 8596 10468 10889 830
Size: 1112 82 1872 421 3478
Fragments arranged by size:
3478 2599 1872 1791 1112 768 543 421
351 317 139 82 64
PvuI CG_AT'CG
Cuts at: 2001 4911 6217 2001
Size: 2910 1306 9321
Fragments arranged by size:
9321 2910 1306
PvuII CAG'CTG
Cuts at: 2407 2480 2864 4823 5602 9572 2407
Size: 73 384 1959 779 3970 6372
Fragments arranged by size:
6372 3970 1959 779 384 73
SacI G_AGCT'C
Cuts at: 4752 6367 7642 7904 10288 4752
Size: 1615 1275 262 2384 8001
Fragments arranged by size:
8001 2384 1615 1275 262
SacII CC_GC'GG
Cuts at: 7177 7177
Size: 13537
SalI G'TCGA_C
Cuts at: 1936 10879 1936
Size: 8943 4594
SanDI GG'GwC_CC
Cuts at: 3743 3860 5007 5955 6010 6109 6154 6230 6625
Size: 117 1147 948 55 99 45 76 395
Cuts at: 6625 6788 7103 7259 7279 7322 7414 7549 8327
Size: 163 315 156 20 43 92 135 778
Cuts at: 8327 9127 9473 9676 9755 9863 10276 10764 3743
Size: 800 346 203 79 108 413 488 6516
Fragments arranged by size:
6516 1147 948 800 778 488 413 395
346 315 203 163 156 135 117 108
99 92 79 76 55 45 43 20
SapI GCTCTTCn'nnn_
Cuts at: 10524 10524
Size: 13537
SbfI CC_TGCA'GG
Cuts at: 8596 10889 8596
Size: 2293 11244
ScaI AGT'ACT
Cuts at: 10559 11785 13497 10559
Size: 1226 1712 10599
Fragments arranged by size:
10599 1712 1226
SexAI A'CCwGG_T
Cuts at: 2416 2466 2715 3987 6241 8053 8566 8842 8967
Size: 50 249 1272 2254 1812 513 276 125
Cuts at: 8967 9769 2416
Size: 802 6184
Fragments arranged by size:
6184 2254 1812 1272 802 513 276 249
125 50
SfcI C'TryA_G
Cuts at: 826 1045 3425 3968 4032 4349 4488 4839 6630
Size: 219 2380 543 64 317 139 351 1791
Cuts at: 6630 7398 8510 8592 10464 10885 12020 12701 12892
Size: 768 1112 82 1872 421 1135 681 191
Cuts at: 12892 826
Size: 1471
Fragments arranged by size:
2380 1872 1791 1471 1135 1112 768 681
543 421 351 317 219 191 139 82
64
SfiI GGCCn_nnn'nGGCC
Cuts at: 5263 6318 6691 7384 7464 7698 8532 8709 9186
Size: 1055 373 693 80 234 834 177 477
Cuts at: 9186 9462 5263
Size: 276 9338
Fragments arranged by size:
9338 1055 834 693 477 373 276 234
177 80
SfoI GGC'GCC
Cuts at: 2058 4138 5462 5616 7594 2058
Size: 2080 1324 154 1978 8001
Fragments arranged by size:
8001 2080 1978 1324 154
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13537
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 13537
SmaI CCC'GGG
Cuts at: 1872 3565 4733 5835 6015 6521 6948 7119 8086
Size: 1693 1168 1102 180 506 427 171 967
Cuts at: 8086 8131 8460 8577 9132 9222 9798 9869 9961
Size: 45 329 117 555 90 576 71 92
Cuts at: 9961 10799 10864 1872
Size: 838 65 4545
Fragments arranged by size:
4545 1693 1168 1102 967 838 576 555
506 427 329 180 171 117 92 90
71 65 45
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2614 2746 3735 5032 5190 5509
Size: 197 926 671 132 989 1297 158 319
Cuts at: 5509 6030 7191 8339 9237 9875 10332 11641 12542
Size: 521 1161 1148 898 638 457 1309 901
Cuts at: 12542 12804 820
Size: 262 1553
Fragments arranged by size:
1553 1309 1297 1161 1148 989 926 901
898 671 638 521 457 319 262 197
158 132
SnaBI TAC'GTA
Cuts at: 493 11328 493
Size: 10835 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 13537
SphI G_CATG'C
Cuts at: 1132 10895 1132
Size: 9763 3774
SrfI GCCC'GGGC
Cuts at: 3565 3565
Size: 13537
SspI AAT'ATT
Cuts at: 5 52 11461 5
Size: 47 11409 2081
Fragments arranged by size:
11409 2081 47
StuI AGG'CCT
Cuts at: 1517 6372 7440 7674 8712 8955 10712 10775 1517
Size: 4855 1068 234 1038 243 1757 63 4279
Fragments arranged by size:
4855 4279 1757 1068 1038 243 234 63
StyI C'CwwG_G
Cuts at: 513 1065 2427 4813 5344 5886 6307 6555 6592
Size: 552 1362 2386 531 542 421 248 37
Cuts at: 6592 6715 6765 7080 7200 7623 7651 7737 7944
Size: 123 50 315 120 423 28 86 207
Cuts at: 7944 8065 8280 8377 8602 9606 10047 10083 10610
Size: 121 215 97 225 1004 441 36 527
Cuts at: 10610 10976 513
Size: 366 3074
Fragments arranged by size:
3074 2386 1362 1004 552 542 531 527
441 423 421 366 315 248 225 215
207 123 121 120 97 86 50 37
36 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2921 3623 3650 6458 7001 8452 8531
Size: 1255 1341 702 27 2808 543 1451 79
Cuts at: 8531 8963 9012 9608 10031 10071 11564 11581 11734
Size: 432 49 596 423 40 1493 17 153
Cuts at: 11734 11919 12364 325
Size: 185 445 1498
Fragments arranged by size:
2808 1498 1493 1451 1341 1255 702 596
543 445 432 423 185 153 79 49
40 27 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2253 2752 2799
Size: 274 80 33 51 1443 276 499 47
Cuts at: 2799 10557 11783 13495 96
Size: 7758 1226 1712 138
Fragments arranged by size:
7758 1712 1443 1226 499 276 274 138
80 51 47 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8812 8844 8892 8924 8955 8987 9775 9807 10242
Size: 32 48 32 31 32 788 32 435
Cuts at: 10242 10274 10434 10466 13497 13529 8812
Size: 32 160 32 3031 32 8820
Fragments arranged by size:
8820 3031 788 435 160 48 32 32
32 32 32 32 32 31
Tth111I GACn'n_nGTC
Cuts at: 1316 2390 5630 1316
Size: 1074 3240 9223
Fragments arranged by size:
9223 3240 1074
XbaI T'CTAG_A
Cuts at: 10873 10873
Size: 13537
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3229 3988 4196 5000 5681 5867 6314 7034 7709
Size: 759 208 804 681 186 447 720 675
Cuts at: 7709 8287 8609 9613 9767 3229
Size: 578 322 1004 154 6999
Fragments arranged by size:
6999 1004 804 759 720 681 675 578
447 322 208 186 154
XhoI C'TCGA_G
Cuts at: 1943 3735 6030 7191 9237 10332 1943
Size: 1792 2295 1161 2046 1095 5148
Fragments arranged by size:
5148 2295 2046 1792 1161 1095
XmaI C'CCGG_G
Cuts at: 1870 3563 4731 5833 6013 6519 6946 7117 8084
Size: 1693 1168 1102 180 506 427 171 967
Cuts at: 8084 8129 8458 8575 9130 9220 9796 9867 9959
Size: 45 329 117 555 90 576 71 92
Cuts at: 9959 10797 10862 1870
Size: 838 65 4545
Fragments arranged by size:
4545 1693 1168 1102 967 838 576 555
506 427 329 180 171 117 92 90
71 65 45
XmnI GAAnn'nnTTC
Cuts at: 7251 10215 11666 7251
Size: 2964 1451 9122
Fragments arranged by size:
9122 2964 1451
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6545 11345 276
Size: 53 83 186 936 5011 4800 2468
Fragments arranged by size:
5011 4800 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BfrBI BplI BsiWI EcoRV
FalI FseI FspAI NruI NsiI PacI PsrI RsrII
SwaI