Restriction map for
pFN21AB4854
(Circular) (Six-base) MAPSORT of: pFN21AB4854.seq Check: 3070 from: 1 to: 8383
pFN21AB4854 8383 bp
With 182 enzymes: SgfI *
January 30, 2014 17:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2611 4356 4438 2611
Size: 1745 82 6556
Fragments arranged by size:
6556 1745 82
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3516 6193 278
Size: 53 83 186 936 1980 2677 2468
Fragments arranged by size:
2677 2468 1980 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5438 5726 1937
Size: 3501 288 4594
Fragments arranged by size:
4594 3501 288
Acc65I G'GTAC_C
Cuts at: 5704 5704
Size: 8383
AclI AA'CG_TT
Cuts at: 1563 6510 6883 1563
Size: 4947 373 3063
Fragments arranged by size:
4947 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2146 2567 2777 4148 4716 5040 6446 7830
Size: 514 421 210 1371 568 324 1406 1384
Cuts at: 7830 8098 1632
Size: 268 1917
Fragments arranged by size:
1917 1406 1384 1371 568 514 421 324
268 210
AflII C'TTAA_G
Cuts at: 820 1017 3113 820
Size: 197 2096 6090
Fragments arranged by size:
6090 2096 197
AflIII A'CryG_T
Cuts at: 1203 7282 8259 1203
Size: 6079 977 1327
Fragments arranged by size:
6079 1327 977
AgeI A'CCGG_T
Cuts at: 7214 7214
Size: 8383
AhdI GACnn_n'nnGTC
Cuts at: 2047 4048 7112 2047
Size: 2001 3064 3318
Fragments arranged by size:
3318 3064 2001
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8383
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3941 4591 1184
Size: 2757 650 4976
Fragments arranged by size:
4976 2757 650
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2625 2657 4211 4243 2625
Size: 32 1554 32 6765
Fragments arranged by size:
6765 1554 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2408 3891 4126 4890 5650 6126 7698 2408
Size: 1483 235 764 760 476 1572 3093
Fragments arranged by size:
3093 1572 1483 764 760 476 235
ApaI G_GGCC'C
Cuts at: 4168 4168
Size: 8383
ApaLI G'TGCA_C
Cuts at: 2780 6440 7596 2780
Size: 3660 1156 3567
Fragments arranged by size:
3660 3567 1156
ApoI r'AATT_y
Cuts at: 1493 2987 5564 5692 5918 6070 8179 1493
Size: 1494 2577 128 226 152 2109 1697
Fragments arranged by size:
2577 2109 1697 1494 226 152 128
AseI AT'TA_AT
Cuts at: 160 1032 6937 160
Size: 872 5905 1606
Fragments arranged by size:
5905 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8383
AvaI C'yCGr_G
Cuts at: 1870 1943 2045 2547 3818 5708 1870
Size: 73 102 502 1271 1890 4545
Fragments arranged by size:
4545 1890 1271 502 102 73
AvrII C'CTAG_G
Cuts at: 4160 4160
Size: 8383
BamHI G'GATC_C
Cuts at: 2208 4335 5713 6080 2208
Size: 2127 1378 367 4511
Fragments arranged by size:
4511 2127 1378 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2460 3223 3800 5704 7159
Size: 325 217 627 673 763 577 1904 1455
Cuts at: 7159 618
Size: 1842
Fragments arranged by size:
1904 1842 1455 763 673 627 577 325
217
BanII G_rGCy'C
Cuts at: 1391 2172 2359 2504 3828 3876 4168 5118 5702
Size: 781 187 145 1324 48 292 950 584
Cuts at: 5702 1391
Size: 4072
Fragments arranged by size:
4072 1324 950 781 584 292 187 145
48
BbeI G_GCGC'C
Cuts at: 3804 3804
Size: 8383
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2757 4488 4903 5178 928
Size: 971 858 1731 415 275 4133
Fragments arranged by size:
4133 1731 971 858 415 275
BbvCI CC'TCA_GC
Cuts at: 3787 3787
Size: 8383
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5013 5047 6574 6608 1903
Size: 34 3076 34 1527 34 3678
Fragments arranged by size:
3678 3076 1527 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3031 6275 7485 3031
Size: 3244 1210 3929
Fragments arranged by size:
3929 3244 1210
BclI T'GATC_A
Cuts at: 1548 4117 1548
Size: 2569 5814
BfrBI ATG'CAT
Cuts at: 2081 4661 2081
Size: 2580 5803
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2612 3617 3769
Size: 107 122 71 631 605 940 1005 152
Cuts at: 3769 6994 136
Size: 3225 1525
Fragments arranged by size:
3225 1525 1005 940 631 605 152 122
107 71
BglII A'GATC_T
Cuts at: 2885 8378 2885
Size: 5493 2890
BlpI GC'TnA_GC
Cuts at: 3119 3968 5800 3119
Size: 849 1832 5702
Fragments arranged by size:
5702 1832 849
Bme1580I G_kGCm'C
Cuts at: 1790 2463 2784 3708 4168 6444 7600 1790
Size: 673 321 924 460 2276 1156 2573
Fragments arranged by size:
2573 2276 1156 924 673 460 321
BmgBI CAC'GTC
Cuts at: 1318 5488 1318
Size: 4170 4213
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5536 6114 7072
Size: 416 53 83 399 24 4119 578 958
Cuts at: 7072 442
Size: 1753
Fragments arranged by size:
4119 1753 958 578 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8383
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3183 3313 3343 3802 3946 3183
Size: 130 30 459 144 7620
Fragments arranged by size:
7620 459 144 130 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2015 2910 3262 3313 3349 4067 4238 4851
Size: 776 895 352 51 36 718 171 613
Cuts at: 4851 7043 1239
Size: 2192 2579
Fragments arranged by size:
2579 2192 895 776 718 613 352 171
51 36
Bpu10I CC'TnA_GC
Cuts at: 2751 3787 2751
Size: 1036 7347
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6508 7373 7671 6508
Size: 865 298 7220
Fragments arranged by size:
7220 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3308 4302 4782 4869 5403 7046 882
Size: 617 1809 994 480 87 534 1643 2219
Fragments arranged by size:
2219 1809 1643 994 617 534 480 87
BsaAI yAC'GTr
Cuts at: 493 1204 6174 493
Size: 711 4970 2702
Fragments arranged by size:
4970 2702 711
BsaBI GATnn'nnATC
Cuts at: 8377 8377
Size: 8383
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3513 3801 6190 6572
Size: 53 83 186 936 1980 288 2389 382
Cuts at: 6572 7207 275
Size: 635 1451
Fragments arranged by size:
2389 1980 1451 936 635 382 288 186
83 53
BsaWI w'CCGG_w
Cuts at: 2205 3299 6816 7214 7488 7635 8046 2205
Size: 1094 3517 398 274 147 411 2542
Fragments arranged by size:
3517 2542 1094 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2514 2544 4211 4241 1178
Size: 30 165 30 1111 30 1667 30 5320
Fragments arranged by size:
5320 1667 1111 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2891 3149 3265 3605 3836 3922 4018 4021
Size: 1705 258 116 340 231 86 96 3
Cuts at: 4021 4076 4096 4235 1186
Size: 55 20 139 5334
Fragments arranged by size:
5334 1705 340 258 231 139 116 96
86 55 20 3
BseYI C'CCAG_C
Cuts at: 2456 2675 3551 3844 4409 7586 8082 2456
Size: 219 876 293 565 3177 496 2757
Fragments arranged by size:
3177 2757 876 565 496 293 219
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2438 2770 5495 5591 5636 1163
Size: 573 702 332 2725 96 45 3910
Fragments arranged by size:
3910 2725 702 573 332 96 45
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2700 3663 3834 4637 4847 5833
Size: 275 186 699 963 171 803 210 986
Cuts at: 5833 6594 7622 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 986 963 803 761 699 275
210 186 171
BsiHKAI G_wGCw'C
Cuts at: 2784 3581 5118 5345 5625 6444 6529 7600 2784
Size: 797 1537 227 280 819 85 1071 3567
Fragments arranged by size:
3567 1537 1071 819 797 280 227 85
BsmI GAATG_Cn'
Cuts at: 5894 5987 5894
Size: 93 8290
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2172 2359 2463 2504 2784 3581 3708
Size: 399 382 187 104 41 280 797 127
Cuts at: 3708 3828 3876 4168 5118 5345 5625 5702 6444
Size: 120 48 292 950 227 280 77 742
Cuts at: 6444 6529 7600 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 950 797 742 399 382 292
280 280 227 187 127 120 104 85
77 48 41
BspEI T'CCGG_A
Cuts at: 3299 8046 3299
Size: 4747 3636
BspHI T'CATG_A
Cuts at: 6272 8002 6272
Size: 1730 6653
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2611 3950 4356 4438 5738 844
Size: 1767 1339 406 82 1300 3489
Fragments arranged by size:
3489 1767 1339 1300 406 82
BsrBI CCG'CTC
Cuts at: 1394 1823 6270 1394
Size: 429 4447 3507
Fragments arranged by size:
4447 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2572 4989 5061 6878 7052 65
Size: 1183 1324 2417 72 1817 174 1396
Fragments arranged by size:
2417 1817 1396 1324 1183 174 72
BsrFI r'CCGG_y
Cuts at: 1700 1810 3382 3813 5596 7027 7214 1700
Size: 110 1572 431 1783 1431 187 2869
Fragments arranged by size:
2869 1783 1572 1431 431 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8383
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8383
BssSI C'ACGA_G
Cuts at: 6443 7455 6443
Size: 1012 7371
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5659 794
Size: 4865 3518
BstBI TT'CG_AA
Cuts at: 7187 7187
Size: 8383
BstEII G'GTnAC_C
Cuts at: 1184 5599 1184
Size: 4415 3968
BstXI CCAn_nnnn'nTGG
Cuts at: 3524 4216 5322 5392 3524
Size: 692 1106 70 6515
Fragments arranged by size:
6515 1106 692 70
BstYI r'GATC_y
Cuts at: 1972 2208 2261 2885 3302 4335 5581 5713 6080
Size: 236 53 624 417 1033 1246 132 367
Cuts at: 6080 6465 6482 7934 8020 8032 8378 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1246 1033 624 417 385 367
346 236 132 86 53 17 12
Bsu36I CC'TnA_GG
Cuts at: 4755 4755
Size: 8383
BtgI C'CryG_G
Cuts at: 513 1065 2004 2607 3192 3469 3657 3663 3669
Size: 552 939 603 585 277 188 6 6
Cuts at: 3669 3717 3750 3828 3834 4089 4859 5070 5321
Size: 48 33 78 6 255 770 211 251
Cuts at: 5321 5391 513
Size: 70 3505
Fragments arranged by size:
3505 939 770 603 585 552 277 255
251 211 188 78 70 48 33 6
6 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4189 487
Size: 683 56 2963 4681
Fragments arranged by size:
4681 2963 683 56
BtsI GCAGTG_nn'
Cuts at: 2782 3130 3708 4159 4573 4936 5899 6693 6713
Size: 348 578 451 414 363 963 794 20
Cuts at: 6713 2782
Size: 4452
Fragments arranged by size:
4452 963 794 578 451 414 363 348
20
ClaI AT'CG_AT
Cuts at: 1554 2328 1554
Size: 774 7609
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8348
DraI TTT'AAA
Cuts at: 5687 5687
Size: 8383
DraIII CAC_nnn'GTG
Cuts at: 2057 2057
Size: 8383
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4012 4327 7390 809
Size: 507 2696 315 3063 1802
Fragments arranged by size:
3063 2696 1802 507 315
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2476 2613 2722
Size: 54 1425 50 275 18 646 137 109
Cuts at: 2722 3660 3666 3831 4249 4539 4844 4879 5478
Size: 938 6 165 418 290 305 35 599
Cuts at: 5478 5535 5547 5830 6719 8348 8
Size: 57 12 283 889 1629 43
Fragments arranged by size:
1629 1425 938 889 646 599 418 305
290 283 275 165 137 109 57 54
50 43 35 18 12 6
EagI C'GGCC_G
Cuts at: 1537 1812 3660 3831 4844 5830 1537
Size: 275 1848 171 1013 986 4090
Fragments arranged by size:
4090 1848 1013 986 275 171
EarI CTCTTCn'nnn_
Cuts at: 1353 2165 3126 4534 4744 6313 1353
Size: 812 961 1408 210 1569 3423
Fragments arranged by size:
3423 1569 1408 961 812 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4800 5239 6970 7344 7490 387
Size: 1064 138 3211 439 1731 374 146 1280
Fragments arranged by size:
3211 1731 1280 1064 439 374 146 138
EcoICRI GAG'CTC
Cuts at: 5116 5116
Size: 8383
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2015 2146 2567 2777 2910 3262 3313
Size: 393 383 131 421 210 133 352 51
Cuts at: 3313 3349 4067 4148 4238 4716 4851 5040 6446
Size: 36 718 81 90 478 135 189 1406
Cuts at: 6446 7043 7830 8098 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1406 787 718 597 478 421 393
383 352 268 210 189 135 133 131
90 81 51 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2030 4352 5549 2030
Size: 2322 1197 4864
Fragments arranged by size:
4864 2322 1197
EcoO109I rG'GnC_Cy
Cuts at: 2041 2511 2734 3619 3783 3888 4164 4165 5066
Size: 470 223 885 164 105 276 1 901
Cuts at: 5066 5153 5647 2041
Size: 87 494 4777
Fragments arranged by size:
4777 901 885 494 470 276 223 164
105 87 1
EcoRI G'AATT_C
Cuts at: 5564 5692 6070 5564
Size: 128 378 7877
Fragments arranged by size:
7877 378 128
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3128 3128
Size: 8383
FspI TGC'GCA
Cuts at: 6889 6889
Size: 8383
HaeII r_GCGC'y
Cuts at: 1678 3192 3804 3955 4377 5427 5673 7530 1678
Size: 1514 612 151 422 1050 246 1857 2531
Fragments arranged by size:
2531 1857 1514 1050 612 422 246 151
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2033 2065 2183 2215 2870
Size: 32 135 32 461 32 118 32 655
Cuts at: 2870 2902 3995 4001 4027 4033 4034 4066 4280
Size: 32 1093 6 26 6 1 32 214
Cuts at: 4280 4282 4312 4314 7024 7056 7098 7130 1373
Size: 2 30 2 2710 32 42 32 2626
Fragments arranged by size:
2710 2626 1093 655 461 214 135 118
42 32 32 32 32 32 32 32
32 30 26 6 6 2 2 1
HincII GTy'rAC
Cuts at: 669 1657 1938 5439 5727 5973 669
Size: 988 281 3501 288 246 3079
Fragments arranged by size:
3501 3079 988 288 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8383
HpaI GTT'AAC
Cuts at: 5973 5973
Size: 8383
KasI G'GCGC_C
Cuts at: 3800 3800
Size: 8383
KpnI G_GTAC'C
Cuts at: 5708 5708
Size: 8383
MfeI C'AATT_G
Cuts at: 5982 5982
Size: 8383
MluI A'CGCG_T
Cuts at: 8259 8259
Size: 8383
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2226 2263 4032 4065 4899 5439 7497 7681
Size: 921 37 1769 33 834 540 2058 184
Cuts at: 7681 1305
Size: 2007
Fragments arranged by size:
2058 2007 1769 921 834 540 184 37
33
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4541 5549 10
Size: 54 1425 343 2709 1008 2844
Fragments arranged by size:
2844 2709 1425 1008 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5320 6341 6700 6859 518
Size: 648 4154 1021 359 159 2042
Fragments arranged by size:
4154 2042 1021 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2408 2631 2904 3261 3406 3659 3719 3752
Size: 802 223 273 357 145 253 60 33
Cuts at: 3752 3830 3836 4139 4771 5799 6476 7624 7869
Size: 78 6 303 632 1028 677 1148 245
Cuts at: 7869 1606
Size: 2120
Fragments arranged by size:
2120 1148 1028 802 677 632 357 303
273 253 245 223 145 78 60 33
6
NarI GG'CG_CC
Cuts at: 3801 3801
Size: 8383
NcoI C'CATG_G
Cuts at: 513 1065 2004 2607 5070 513
Size: 552 939 603 2463 3826
Fragments arranged by size:
3826 2463 939 603 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8383
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8383
NotI GC'GGCC_GC
Cuts at: 3831 5830 3831
Size: 1999 6384
NsiI A_TGCA'T
Cuts at: 2083 4663 2083
Size: 2580 5803
NspI r_CATG'y
Cuts at: 1132 1229 5741 7286 1132
Size: 97 4512 1545 2229
Fragments arranged by size:
4512 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3623 4510 1348
Size: 2275 887 5221
Fragments arranged by size:
5221 2275 887
PciI A'CATG_T
Cuts at: 7282 7282
Size: 8383
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3077 5473 1108
Size: 1969 2396 4018
Fragments arranged by size:
4018 2396 1969
PfoI T'CCnGG_A
Cuts at: 2031 2922 3516 4109 4331 2031
Size: 891 594 593 222 6083
Fragments arranged by size:
6083 891 594 593 222
PmeI GTTT'AAAC
Cuts at: 5687 5687
Size: 8383
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2354 2386 4211 4243 6452 6484 7965 7997 2354
Size: 32 1825 32 2209 32 1481 32 2740
Fragments arranged by size:
2740 2209 1825 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2041 2511 3783 2041
Size: 470 1272 6641
Fragments arranged by size:
6641 1272 470
PshAI GACnn'nnGTC
Cuts at: 1613 5444 1613
Size: 3831 4552
PsiI TTA'TAA
Cuts at: 5953 5953
Size: 8383
PspOMI G'GGCC_C
Cuts at: 4164 4164
Size: 8383
PspXI vC'TCGA_Gb
Cuts at: 2045 2045
Size: 8383
PstI C_TGCA'G
Cuts at: 830 3215 4435 4776 5735 830
Size: 2385 1220 341 959 3478
Fragments arranged by size:
3478 2385 1220 959 341
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8383
PvuII CAG'CTG
Cuts at: 2408 2904 3406 4771 2408
Size: 496 502 1365 6020
Fragments arranged by size:
6020 1365 502 496
SacI G_AGCT'C
Cuts at: 5118 5118
Size: 8383
SacII CC_GC'GG
Cuts at: 3660 3720 3753 3831 3837 3660
Size: 60 33 78 6 8206
Fragments arranged by size:
8206 78 60 33 6
SalI G'TCGA_C
Cuts at: 1936 5437 5725 1936
Size: 3501 288 4594
Fragments arranged by size:
4594 3501 288
SapI GCTCTTCn'nnn_
Cuts at: 4534 4534
Size: 8383
SbfI CC_TGCA'GG
Cuts at: 5735 5735
Size: 8383
ScaI AGT'ACT
Cuts at: 3491 5636 6631 8343 3491
Size: 2145 995 1712 3531
Fragments arranged by size:
3531 2145 1712 995
SexAI A'CCwGG_T
Cuts at: 5625 5625
Size: 8383
SfcI C'TryA_G
Cuts at: 826 1045 2380 3211 4170 4431 4489 4772 5731
Size: 219 1335 831 959 261 58 283 959
Cuts at: 5731 6866 7547 7738 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1335 1135 959 959 831 681 283
261 219 191 58
SfoI GGC'GCC
Cuts at: 3802 3802
Size: 8383
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8383
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8383
SmaI CCC'GGG
Cuts at: 1872 5710 1872
Size: 3838 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2045 3113 6487 7388 7650 820
Size: 197 926 102 1068 3374 901 262 1553
Fragments arranged by size:
3374 1553 1068 926 901 262 197 102
SnaBI TAC'GTA
Cuts at: 493 6174 493
Size: 5681 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8383
SphI G_CATG'C
Cuts at: 1132 5741 1132
Size: 4609 3774
SspI AAT'ATT
Cuts at: 5 52 6307 5
Size: 47 6255 2081
Fragments arranged by size:
6255 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8383
StyI C'CwwG_G
Cuts at: 513 1065 2004 2607 2835 3602 3990 4160 4209
Size: 552 939 603 228 767 388 170 49
Cuts at: 4209 4386 5070 5550 5822 513
Size: 177 684 480 272 3074
Fragments arranged by size:
3074 939 767 684 603 552 480 388
272 228 177 170 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2478 3332 4623 6410 6427 6580 6765
Size: 1255 898 854 1291 1787 17 153 185
Cuts at: 6765 7210 325
Size: 445 1498
Fragments arranged by size:
1787 1498 1291 1255 898 854 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3489 5217 5634
Size: 274 80 33 51 1443 1512 1728 417
Cuts at: 5634 6629 8341 96
Size: 995 1712 138
Fragments arranged by size:
1728 1712 1512 1443 995 417 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4183 4215 5448 5480 8343 8375 4183
Size: 32 1233 32 2863 32 4191
Fragments arranged by size:
4191 2863 1233 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4009 1316
Size: 2693 5690
XbaI T'CTAG_A
Cuts at: 5719 5719
Size: 8383
XhoI C'TCGA_G
Cuts at: 1943 2045 1943
Size: 102 8281
XmaI C'CCGG_G
Cuts at: 1870 5708 1870
Size: 3838 4545
XmnI GAAnn'nnTTC
Cuts at: 4824 6512 4824
Size: 1688 6695
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3514 6191 276
Size: 53 83 186 936 1980 2677 2468
Fragments arranged by size:
2677 2468 1980 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BsiWI BsmBI BstZ17I EcoRV FseI
FspAI NaeI NgoMIV NruI PacI PmlI PsrI RsrII
SanDI SfiI SrfI SwaI XcmI