Restriction map for
pFN21AB4846
(Circular) (Six-base) MAPSORT of: pFN21AB4846.seq Check: 206 from: 1 to: 6145
pFN21AB4846 6145 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2097 3955 278
Size: 53 83 186 936 561 1858 2468
Fragments arranged by size:
2468 1858 936 561 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2962 3488 1937
Size: 1025 526 4594
Fragments arranged by size:
4594 1025 526
Acc65I G'GTAC_C
Cuts at: 3466 3466
Size: 6145
AclI AA'CG_TT
Cuts at: 1563 4272 4645 1563
Size: 2709 373 3063
Fragments arranged by size:
3063 2709 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 1995 4208 5592 5860 1632
Size: 363 2213 1384 268 1917
Fragments arranged by size:
2213 1917 1384 363 268
AfeI AGC'GCT
Cuts at: 3272 3272
Size: 6145
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5948
AflIII A'CryG_T
Cuts at: 1203 5044 6021 1203
Size: 3841 977 1327
Fragments arranged by size:
3841 1327 977
AgeI A'CCGG_T
Cuts at: 4976 4976
Size: 6145
AhdI GACnn_n'nnGTC
Cuts at: 4874 4874
Size: 6145
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6145
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6145
AlwNI CAG_nnn'CTG
Cuts at: 3888 5460 3888
Size: 1572 4573
ApaI G_GGCC'C
Cuts at: 2604 2604
Size: 6145
ApaLI G'TGCA_C
Cuts at: 2891 4202 5358 2891
Size: 1311 1156 3678
Fragments arranged by size:
3678 1311 1156
ApoI r'AATT_y
Cuts at: 1493 3454 3680 3832 5941 1493
Size: 1961 226 152 2109 1697
Fragments arranged by size:
2109 1961 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4699 160
Size: 872 3667 1606
Fragments arranged by size:
3667 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
AvaI C'yCGr_G
Cuts at: 1870 1943 2260 2604 2651 3088 3470 1870
Size: 73 317 344 47 437 382 4545
Fragments arranged by size:
4545 437 382 344 317 73 47
AvrII C'CTAG_G
Cuts at: 2365 2365
Size: 6145
BamHI G'GATC_C
Cuts at: 2870 3475 3842 2870
Size: 605 367 5173
Fragments arranged by size:
5173 605 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2180 2974 3056 3227 3403
Size: 325 217 627 393 794 82 171 176
Cuts at: 3403 3466 4921 618
Size: 63 1455 1842
Fragments arranged by size:
1842 1455 794 627 393 325 217 176
171 82 63
BanII G_rGCy'C
Cuts at: 1391 2150 2604 3122 3464 1391
Size: 759 454 518 342 4072
Fragments arranged by size:
4072 759 518 454 342
BbeI G_GCGC'C
Cuts at: 2184 3060 3231 3407 2184
Size: 876 171 176 4922
Fragments arranged by size:
4922 876 176 171
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2533 2623 928
Size: 971 634 90 4450
Fragments arranged by size:
4450 971 634 90
BbvCI CC'TCA_GC
Cuts at: 2216 2787 2216
Size: 571 5574
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3082 3116 4336 4370 1903
Size: 34 1145 34 1220 34 3678
Fragments arranged by size:
3678 1220 1145 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2803 4037 5247 2803
Size: 1234 1210 3701
Fragments arranged by size:
3701 1234 1210
BclI T'GATC_A
Cuts at: 1548 2638 1548
Size: 1090 5055
BfrBI ATG'CAT
Cuts at: 2067 2067
Size: 6145
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4756 136
Size: 107 122 71 631 605 3084 1525
Fragments arranged by size:
3084 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6140 6140
Size: 6145
BlpI GC'TnA_GC
Cuts at: 2144 2430 3562 2144
Size: 286 1132 4727
Fragments arranged by size:
4727 1132 286
Bme1580I G_kGCm'C
Cuts at: 1790 2604 2895 4206 5362 1790
Size: 814 291 1311 1156 2573
Fragments arranged by size:
2573 1311 1156 814 291
BmgBI CAC'GTC
Cuts at: 1318 2921 3358 1318
Size: 1603 437 4105
Fragments arranged by size:
4105 1603 437
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3876 4834 442
Size: 416 53 83 399 24 2459 958 1753
Fragments arranged by size:
2459 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6145
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2708 3440 4805 1239
Size: 1469 732 1365 2579
Fragments arranged by size:
2579 1469 1365 732
Bpu10I CC'TnA_GC
Cuts at: 2216 2787 2216
Size: 571 5574
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4270 5135 5433 4270
Size: 865 298 4982
Fragments arranged by size:
4982 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4808 882
Size: 617 3309 2219
Fragments arranged by size:
3309 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3936 493
Size: 711 2732 2702
Fragments arranged by size:
2732 2702 711
BsaBI GATnn'nnATC
Cuts at: 6139 6139
Size: 6145
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2094 2168 2181 2190
Size: 53 83 186 936 561 74 13 9
Cuts at: 2190 3057 3077 3228 3404 3952 4334 4969 275
Size: 867 20 151 176 548 382 635 1451
Fragments arranged by size:
1451 936 867 635 561 548 382 186
176 151 83 74 53 20 13 9
BsaWI w'CCGG_w
Cuts at: 4578 4976 5250 5397 5808 4578
Size: 398 274 147 411 4915
Fragments arranged by size:
4915 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3236 3266 3371 3401 3416
Size: 30 165 30 1833 30 105 30 15
Cuts at: 3416 3446 1178
Size: 30 3877
Fragments arranged by size:
3877 1833 165 105 30 30 30 30
30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2233 3437 1186
Size: 1047 1204 3894
Fragments arranged by size:
3894 1204 1047
BseYI C'CCAG_C
Cuts at: 2176 2731 2896 3335 5348 5844 2176
Size: 555 165 439 2013 496 2477
Fragments arranged by size:
2477 2013 555 496 439 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3283 1163
Size: 573 1547 4025
Fragments arranged by size:
4025 1547 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2230 2500 3237 3595 4356 5384
Size: 275 186 229 270 737 358 761 1028
Cuts at: 5384 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 737 358 275 270 229
186
BsiHKAI G_wGCw'C
Cuts at: 2895 4206 4291 5362 2895
Size: 1311 85 1071 3678
Fragments arranged by size:
3678 1311 1071 85
BsmI GAATG_Cn'
Cuts at: 2069 3656 3749 2069
Size: 1587 93 4465
Fragments arranged by size:
4465 1587 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2085 2085
Size: 6145
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2150 2604 2895 3122 3464 4206 4291
Size: 399 360 454 291 227 342 742 85
Cuts at: 4291 5362 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 454 399 360 342 291
227 85
BspEI T'CCGG_A
Cuts at: 5808 5808
Size: 6145
BspHI T'CATG_A
Cuts at: 2055 4034 5764 2055
Size: 1979 1730 2436
Fragments arranged by size:
2436 1979 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3153 3500 844
Size: 2309 347 3489
Fragments arranged by size:
3489 2309 347
BsrBI CCG'CTC
Cuts at: 1394 1823 2223 2674 4032 1394
Size: 429 400 451 1358 3507
Fragments arranged by size:
3507 1358 451 429 400
BsrDI GCAATG_nn'
Cuts at: 65 1248 2416 2874 4640 4814 65
Size: 1183 1168 458 1766 174 1396
Fragments arranged by size:
1766 1396 1183 1168 458 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2192 2207 3079 3236 3314 4789 4976
Size: 110 382 15 872 157 78 1475 187
Cuts at: 4976 1700
Size: 2869
Fragments arranged by size:
2869 1475 872 382 187 157 110 78
15
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6145
BssHII G'CGCG_C
Cuts at: 1925 3413 1925
Size: 1488 4657
BssSI C'ACGA_G
Cuts at: 2110 2751 4205 5217 2110
Size: 641 1454 1012 3038
Fragments arranged by size:
3038 1454 1012 641
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6145
BstBI TT'CG_AA
Cuts at: 4949 4949
Size: 6145
BstEII G'GTnAC_C
Cuts at: 1184 3082 1184
Size: 1898 4247
BstXI CCAn_nnnn'nTGG
Cuts at: 3400 3400
Size: 6145
BstYI r'GATC_y
Cuts at: 1972 2820 2870 3475 3842 4227 4244 5696 5782
Size: 848 50 605 367 385 17 1452 86
Cuts at: 5782 5794 6140 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 848 605 385 367 346 86
50 17 12
Bsu36I CC'TnA_GG
Cuts at: 2486 2486
Size: 6145
BtgI C'CryG_G
Cuts at: 513 1065 2004 2837 3114 3255 513
Size: 552 939 833 277 141 3403
Fragments arranged by size:
3403 939 833 552 277 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2329 487
Size: 683 56 1103 4303
Fragments arranged by size:
4303 1103 683 56
BtsI GCAGTG_nn'
Cuts at: 2772 3661 4455 4475 2772
Size: 889 794 20 4442
Fragments arranged by size:
4442 889 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6145
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6110
DraI TTT'AAA
Cuts at: 3449 3449
Size: 6145
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5152 809
Size: 507 3836 1802
Fragments arranged by size:
3836 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2205 2250 3111
Size: 54 1425 50 275 18 375 45 861
Cuts at: 3111 3123 3234 3592 4481 6110 8
Size: 12 111 358 889 1629 43
Fragments arranged by size:
1629 1425 889 861 375 358 275 111
54 50 45 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 3234 3592 1537
Size: 275 1422 358 4090
Fragments arranged by size:
4090 1422 358 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2503 4075 1353
Size: 1150 1572 3423
Fragments arranged by size:
3423 1572 1150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4732 5106 5252 387
Size: 1064 138 3143 374 146 1280
Fragments arranged by size:
3143 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1995 2708 3440 4208 4805 5592 5860
Size: 393 363 713 732 768 597 787 268
Cuts at: 5860 1239
Size: 1524
Fragments arranged by size:
1524 787 768 732 713 597 393 363
268
EcoNI CCTnn'n_nnAGG
Cuts at: 3422 3422
Size: 6145
EcoO109I rG'GnC_Cy
Cuts at: 2601 3438 2601
Size: 837 5308
EcoRI G'AATT_C
Cuts at: 3454 3832 3454
Size: 378 5767
FspI TGC'GCA
Cuts at: 2276 4651 2276
Size: 2375 3770
FspAI rTGC'GCAy
Cuts at: 2276 2276
Size: 6145
HaeII r_GCGC'y
Cuts at: 1678 2184 2199 2326 3060 3231 3274 3407 5292
Size: 506 15 127 734 171 43 133 1885
Cuts at: 5292 1678
Size: 2531
Fragments arranged by size:
2531 1885 734 506 171 133 127 43
15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4786 4818 4860 4892 1373
Size: 32 135 32 3214 32 42 32 2626
Fragments arranged by size:
3214 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2963 3489 3735 669
Size: 988 281 1025 526 246 3079
Fragments arranged by size:
3079 1025 988 526 281 246
HindIII A'AGCT_T
Cuts at: 748 2518 748
Size: 1770 4375
HpaI GTT'AAC
Cuts at: 3735 3735
Size: 6145
KasI G'GCGC_C
Cuts at: 2180 3056 3227 3403 2180
Size: 876 171 176 4922
Fragments arranged by size:
4922 876 176 171
KpnI G_GTAC'C
Cuts at: 3470 3470
Size: 6145
MfeI C'AATT_G
Cuts at: 3744 3744
Size: 6145
MluI A'CGCG_T
Cuts at: 6021 6021
Size: 6145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2848 3057 5259 5443 1305
Size: 1543 209 2202 184 2007
Fragments arranged by size:
2202 2007 1543 209 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4323
Fragments arranged by size:
4323 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2399 2708 3104 3398 4103 4462 4621
Size: 648 1233 309 396 294 705 359 159
Cuts at: 4621 518
Size: 2042
Fragments arranged by size:
2042 1233 705 648 396 359 309 294
159
MspA1I CmG'CkG
Cuts at: 1606 2176 2695 2928 3561 4238 5386 5631 1606
Size: 570 519 233 633 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 633 570 519 245 233
NaeI GCC'GGC
Cuts at: 2194 2209 3238 2194
Size: 15 1029 5101
Fragments arranged by size:
5101 1029 15
NarI GG'CG_CC
Cuts at: 2181 3057 3228 3404 2181
Size: 876 171 176 4922
Fragments arranged by size:
4922 876 176 171
NcoI C'CATG_G
Cuts at: 513 1065 2004 3114 3255 513
Size: 552 939 1110 141 3403
Fragments arranged by size:
3403 1110 939 552 141
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6145
NgoMIV G'CCGG_C
Cuts at: 2192 2207 3236 2192
Size: 15 1029 5101
Fragments arranged by size:
5101 1029 15
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6145
NotI GC'GGCC_GC
Cuts at: 3592 3592
Size: 6145
NsiI A_TGCA'T
Cuts at: 2069 2069
Size: 6145
NspI r_CATG'y
Cuts at: 1132 1229 3046 3109 3503 5048 1132
Size: 97 1817 63 394 1545 2229
Fragments arranged by size:
2229 1817 1545 394 97 63
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6145
PciI A'CATG_T
Cuts at: 5044 5044
Size: 6145
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2866 1108
Size: 1758 4387
PmeI GTTT'AAAC
Cuts at: 3449 3449
Size: 6145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4214 4246 5727 5759 4214
Size: 32 1481 32 4600
Fragments arranged by size:
4600 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3080 1613
Size: 1467 4678
PsiI TTA'TAA
Cuts at: 3715 3715
Size: 6145
PspOMI G'GGCC_C
Cuts at: 2600 2600
Size: 6145
PstI C_TGCA'G
Cuts at: 830 2950 3497 830
Size: 2120 547 3478
Fragments arranged by size:
3478 2120 547
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6145
PvuII CAG'CTG
Cuts at: 2695 2695
Size: 6145
SalI G'TCGA_C
Cuts at: 1936 2961 3487 1936
Size: 1025 526 4594
Fragments arranged by size:
4594 1025 526
SbfI CC_TGCA'GG
Cuts at: 3497 3497
Size: 6145
ScaI AGT'ACT
Cuts at: 4393 6105 4393
Size: 1712 4433
SexAI A'CCwGG_T
Cuts at: 2463 2463
Size: 6145
SfcI C'TryA_G
Cuts at: 826 1045 2946 3493 4628 5309 5500 826
Size: 219 1901 547 1135 681 191 1471
Fragments arranged by size:
1901 1471 1135 681 547 219 191
SfoI GGC'GCC
Cuts at: 2182 3058 3229 3405 2182
Size: 876 171 176 4922
Fragments arranged by size:
4922 876 176 171
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
SgrAI Cr'CCGG_yG
Cuts at: 1700 2192 1700
Size: 492 5653
SmaI CCC'GGG
Cuts at: 1872 2262 2653 3472 1872
Size: 390 391 819 4545
Fragments arranged by size:
4545 819 391 390
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4249 5150 5412 820
Size: 197 926 2306 901 262 1553
Fragments arranged by size:
2306 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3936 493
Size: 3443 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6145
SphI G_CATG'C
Cuts at: 1132 3046 3503 1132
Size: 1914 457 3774
Fragments arranged by size:
3774 1914 457
SspI AAT'ATT
Cuts at: 5 52 4069 5
Size: 47 4017 2081
Fragments arranged by size:
4017 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6145
StyI C'CwwG_G
Cuts at: 513 1065 2004 2365 3114 3255 3584 513
Size: 552 939 361 749 141 329 3074
Fragments arranged by size:
3074 939 749 552 361 329 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2486 2745 2910 4172 4189 4342 4527
Size: 1255 906 259 165 1262 17 153 185
Cuts at: 4527 4972 325
Size: 445 1498
Fragments arranged by size:
1498 1262 1255 906 445 259 185 165
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3219 4391 6103
Size: 274 80 33 51 1443 1242 1172 1712
Cuts at: 6103 96
Size: 138
Fragments arranged by size:
1712 1443 1242 1172 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3371 3403 3416 3448 6105 6137 3371
Size: 32 13 32 2657 32 3379
Fragments arranged by size:
3379 2657 32 32 32 13
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6145
XbaI T'CTAG_A
Cuts at: 2087 3481 2087
Size: 1394 4751
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2835 2835
Size: 6145
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6145
XmaI C'CCGG_G
Cuts at: 1870 2260 2651 3470 1870
Size: 390 391 819 4545
Fragments arranged by size:
4545 819 391 390
XmnI GAAnn'nnTTC
Cuts at: 2519 4274 2519
Size: 1755 4390
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2095 3953 276
Size: 53 83 186 936 561 1858 2468
Fragments arranged by size:
2468 1858 936 561 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BplI BsiWI BstZ17I DraIII
EcoICRI EcoRV FalI FseI NruI PacI PfoI PmlI
PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SrfI SwaI