Restriction map for
pFN21AB4832
(Circular) (Six-base) MAPSORT of: pFN21AB4832.seq Check: 5977 from: 1 to: 5971
pFN21AB4832 5971 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2178 2637 3781 278
Size: 53 83 186 936 642 459 1144 2468
Fragments arranged by size:
2468 1144 936 642 459 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2178 3314 1937
Size: 241 1136 4594
Fragments arranged by size:
4594 1136 241
Acc65I G'GTAC_C
Cuts at: 3292 3292
Size: 5971
AclI AA'CG_TT
Cuts at: 1563 4098 4471 1563
Size: 2535 373 3063
Fragments arranged by size:
3063 2535 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2570 2872 4034 5418 5686 1632
Size: 938 302 1162 1384 268 1917
Fragments arranged by size:
1917 1384 1162 938 302 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5774
AflIII A'CryG_T
Cuts at: 1203 4870 5847 1203
Size: 3667 977 1327
Fragments arranged by size:
3667 1327 977
AgeI A'CCGG_T
Cuts at: 4802 4802
Size: 5971
AhdI GACnn_n'nnGTC
Cuts at: 4700 4700
Size: 5971
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5971
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5971
AlwNI CAG_nnn'CTG
Cuts at: 2406 3178 3250 3714 5286 2406
Size: 772 72 464 1572 3091
Fragments arranged by size:
3091 1572 772 464 72
ApaLI G'TGCA_C
Cuts at: 4028 5184 4028
Size: 1156 4815
ApoI r'AATT_y
Cuts at: 1493 2603 3280 3506 3658 5767 1493
Size: 1110 677 226 152 2109 1697
Fragments arranged by size:
2109 1697 1110 677 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2775 4525 160
Size: 872 1743 1750 1606
Fragments arranged by size:
1750 1743 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
AvaI C'yCGr_G
Cuts at: 1870 1943 3191 3296 1870
Size: 73 1248 105 4545
Fragments arranged by size:
4545 1248 105 73
BamHI G'GATC_C
Cuts at: 2955 3301 3668 2955
Size: 346 367 5258
Fragments arranged by size:
5258 367 346
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2486 2508 3292 4747 618
Size: 325 217 627 699 22 784 1455 1842
Fragments arranged by size:
1842 1455 784 699 627 325 217 22
BanII G_rGCy'C
Cuts at: 1391 2031 2711 3258 3290 1391
Size: 640 680 547 32 4072
Fragments arranged by size:
4072 680 640 547 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2453 2568 3084 928
Size: 971 554 115 516 3815
Fragments arranged by size:
3815 971 554 516 115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4162 4196 1903
Size: 34 2225 34 3678
Fragments arranged by size:
3678 2225 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3863 5073 3863
Size: 1210 4761
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5971
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2670 4582 136
Size: 107 122 71 631 605 998 1912 1525
Fragments arranged by size:
1912 1525 998 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2496 2645 5966 2496
Size: 149 3321 2501
Fragments arranged by size:
3321 2501 149
BlpI GC'TnA_GC
Cuts at: 3388 3388
Size: 5971
Bme1580I G_kGCm'C
Cuts at: 1790 2489 2513 3242 4032 5188 1790
Size: 699 24 729 790 1156 2573
Fragments arranged by size:
2573 1156 790 729 699 24
BmgBI CAC'GTC
Cuts at: 1318 2968 1318
Size: 1650 4321
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2714 3702 4660
Size: 416 53 83 399 24 1297 988 958
Cuts at: 4660 442
Size: 1753
Fragments arranged by size:
1753 1297 988 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5971
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2542 2542
Size: 5971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2512 2774 3272 4631 1239
Size: 1273 262 498 1359 2579
Fragments arranged by size:
2579 1359 1273 498 262
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4096 4961 5259 4096
Size: 865 298 4808
Fragments arranged by size:
4808 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4634 882
Size: 617 3135 2219
Fragments arranged by size:
3135 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3762 493
Size: 711 2558 2702
Fragments arranged by size:
2702 2558 711
BsaBI GATnn'nnATC
Cuts at: 5965 5965
Size: 5971
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2175 2634 3778 4160
Size: 53 83 186 936 642 459 1144 382
Cuts at: 4160 4795 275
Size: 635 1451
Fragments arranged by size:
1451 1144 936 642 635 459 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4404 4802 5076 5223 5634 4404
Size: 398 274 147 411 4741
Fragments arranged by size:
4741 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2286 2316 1178
Size: 30 165 30 883 30 4833
Fragments arranged by size:
4833 883 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2552 2863 1186
Size: 1366 311 4294
Fragments arranged by size:
4294 1366 311
BseYI C'CCAG_C
Cuts at: 2414 2721 3115 3154 5174 5670 2414
Size: 307 394 39 2020 496 2715
Fragments arranged by size:
2715 2020 496 394 307 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5398
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2455 3421 4182 5210 1540
Size: 275 186 454 966 761 1028 2301
Fragments arranged by size:
2301 1028 966 761 454 275 186
BsiHKAI G_wGCw'C
Cuts at: 2393 3269 4032 4117 5188 2393
Size: 876 763 85 1071 3176
Fragments arranged by size:
3176 1071 876 763 85
BsmI GAATG_Cn'
Cuts at: 2373 3482 3575 2373
Size: 1109 93 4769
Fragments arranged by size:
4769 1109 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2904 2904
Size: 5971
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2393 2489 2513 2711 3242 3258
Size: 399 241 362 96 24 198 531 16
Cuts at: 3258 3269 3290 4032 4117 5188 1391
Size: 11 21 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 531 399 362 241 198
96 85 24 21 16 11
BspEI T'CCGG_A
Cuts at: 5634 5634
Size: 5971
BspHI T'CATG_A
Cuts at: 3860 5590 3860
Size: 1730 4241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3326 844
Size: 2482 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3858 1394
Size: 429 2035 3507
Fragments arranged by size:
3507 2035 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2629 4466 4640 65
Size: 1183 1381 1837 174 1396
Fragments arranged by size:
1837 1396 1381 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2103 2450 4615 4802 1700
Size: 110 293 347 2165 187 2869
Fragments arranged by size:
2869 2165 347 293 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5971
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5971
BssSI C'ACGA_G
Cuts at: 4031 5043 4031
Size: 1012 4959
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3178 794
Size: 2384 3587
BstBI TT'CG_AA
Cuts at: 4775 4775
Size: 5971
BstEII G'GTnAC_C
Cuts at: 1184 2165 2314 1184
Size: 981 149 4841
Fragments arranged by size:
4841 981 149
BstYI r'GATC_y
Cuts at: 1972 2242 2256 2496 2645 2955 3230 3301 3668
Size: 270 14 240 149 310 275 71 367
Cuts at: 3668 4053 4070 5522 5608 5620 5966 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 310 275 270
240 149 86 71 17 14 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4480
Fragments arranged by size:
4480 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2056 487
Size: 683 56 830 4402
Fragments arranged by size:
4402 830 683 56
BtsI GCAGTG_nn'
Cuts at: 3487 4281 4301 3487
Size: 794 20 5157
Fragments arranged by size:
5157 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5971
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5936
DraI TTT'AAA
Cuts at: 3275 3275
Size: 5971
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4978 809
Size: 507 3662 1802
Fragments arranged by size:
3662 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2452 2478 3418
Size: 54 1425 50 275 18 622 26 940
Cuts at: 3418 4307 5936 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 940 889 622 275 54 50
43 26 18
EagI C'GGCC_G
Cuts at: 1537 1812 2452 3418 1537
Size: 275 640 966 4090
Fragments arranged by size:
4090 966 640 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3901 1353
Size: 2548 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4558 4932 5078 387
Size: 1064 138 2969 374 146 1280
Fragments arranged by size:
2969 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2512 2570 2774 2872 3272 4034 4631
Size: 393 880 58 204 98 400 762 597
Cuts at: 4631 5418 5686 1239
Size: 787 268 1524
Fragments arranged by size:
1524 880 787 762 597 400 393 268
204 98 58
EcoNI CCTnn'n_nnAGG
Cuts at: 2552 2552
Size: 5971
EcoO109I rG'GnC_Cy
Cuts at: 3070 3070
Size: 5971
EcoRI G'AATT_C
Cuts at: 3280 3658 3280
Size: 378 5593
FspI TGC'GCA
Cuts at: 4477 4477
Size: 5971
HaeII r_GCGC'y
Cuts at: 1678 5118 1678
Size: 3440 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2286 2318 3092 3124 4612
Size: 32 135 32 714 32 774 32 1488
Cuts at: 4612 4644 4686 4718 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1488 774 714 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2179 3315 3561 669
Size: 988 281 241 1136 246 3079
Fragments arranged by size:
3079 1136 988 281 246 241
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5971
HpaI GTT'AAC
Cuts at: 3561 3561
Size: 5971
KpnI G_GTAC'C
Cuts at: 3296 3296
Size: 5971
MfeI C'AATT_G
Cuts at: 3570 3570
Size: 5971
MluI A'CGCG_T
Cuts at: 5847 5847
Size: 5971
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2496 2725 5085 5269 1305
Size: 1191 229 2360 184 2007
Fragments arranged by size:
2360 2007 1191 229 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2480 10
Size: 54 1425 343 648 3501
Fragments arranged by size:
3501 1425 648 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2939 3929 4288 4447 518
Size: 648 1773 990 359 159 2042
Fragments arranged by size:
2042 1773 990 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2254 2344 2432 2457 2721 3387 4064 5212
Size: 648 90 88 25 264 666 677 1148
Cuts at: 5212 5457 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 666 648 264 245 90
88 25
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4480
Fragments arranged by size:
4480 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5971
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5971
NotI GC'GGCC_GC
Cuts at: 3418 3418
Size: 5971
NspI r_CATG'y
Cuts at: 1132 1229 3329 4874 1132
Size: 97 2100 1545 2229
Fragments arranged by size:
2229 2100 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2290 1348
Size: 942 5029
PciI A'CATG_T
Cuts at: 4870 4870
Size: 5971
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5971
PfoI T'CCnGG_A
Cuts at: 2739 3249 2739
Size: 510 5461
PmeI GTTT'AAAC
Cuts at: 3275 3275
Size: 5971
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4040 4072 5553 5585 4040
Size: 32 1481 32 4426
Fragments arranged by size:
4426 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5971
PsiI TTA'TAA
Cuts at: 3541 3541
Size: 5971
PstI C_TGCA'G
Cuts at: 830 2115 2193 3183 3323 830
Size: 1285 78 990 140 3478
Fragments arranged by size:
3478 1285 990 140 78
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5971
PvuII CAG'CTG
Cuts at: 2432 2432
Size: 5971
SalI G'TCGA_C
Cuts at: 1936 2177 3313 1936
Size: 241 1136 4594
Fragments arranged by size:
4594 1136 241
SbfI CC_TGCA'GG
Cuts at: 3323 3323
Size: 5971
ScaI AGT'ACT
Cuts at: 4219 5931 4219
Size: 1712 4259
SexAI A'CCwGG_T
Cuts at: 2133 3003 2133
Size: 870 5101
SfcI C'TryA_G
Cuts at: 826 1045 2111 2189 3179 3319 4454 5135 5326
Size: 219 1066 78 990 140 1135 681 191
Cuts at: 5326 826
Size: 1471
Fragments arranged by size:
1471 1135 1066 990 681 219 191 140
78
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5971
SmaI CCC'GGG
Cuts at: 1872 3298 1872
Size: 1426 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4075 4976 5238 820
Size: 197 926 2132 901 262 1553
Fragments arranged by size:
2132 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3762 493
Size: 3269 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5971
SphI G_CATG'C
Cuts at: 1132 3329 1132
Size: 2197 3774
SspI AAT'ATT
Cuts at: 5 52 2779 3895 5
Size: 47 2727 1116 2081
Fragments arranged by size:
2727 2081 1116 47
StuI AGG'CCT
Cuts at: 1517 2117 1517
Size: 600 5371
StyI C'CwwG_G
Cuts at: 513 1065 2004 2394 3410 513
Size: 552 939 390 1016 3074
Fragments arranged by size:
3074 1016 939 552 390
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2072 2134 3998 4015 4168 4353 4798
Size: 1255 492 62 1864 17 153 185 445
Cuts at: 4798 325
Size: 1498
Fragments arranged by size:
1864 1498 1255 492 445 185 153 62
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4217 5929 96
Size: 274 80 33 51 1443 2240 1712 138
Fragments arranged by size:
2240 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2427 2459 5931 5963 2427
Size: 32 3472 32 2435
Fragments arranged by size:
3472 2435 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2276 1316
Size: 960 5011
XbaI T'CTAG_A
Cuts at: 3307 3307
Size: 5971
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2701 2701
Size: 5971
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5971
XmaI C'CCGG_G
Cuts at: 1870 3296 1870
Size: 1426 4545
XmnI GAAnn'nnTTC
Cuts at: 2373 4100 2373
Size: 1727 4244
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2176 2635 3779 276
Size: 53 83 186 936 642 459 1144 2468
Fragments arranged by size:
2468 1144 936 642 459 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI