Restriction map for
pFN21AB4828
(Circular) (Six-base) MAPSORT of: pFN21AB4828.seq Check: 2021 from: 1 to: 5578
pFN21AB4828 5578 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2450 3388 278
Size: 53 83 186 936 914 938 2468
Fragments arranged by size:
2468 938 936 914 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2067 2921 1937
Size: 130 854 4594
Fragments arranged by size:
4594 854 130
Acc65I G'GTAC_C
Cuts at: 2899 2899
Size: 5578
AclI AA'CG_TT
Cuts at: 1563 3705 4078 1563
Size: 2142 373 3063
Fragments arranged by size:
3063 2142 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2432 2868 3641 5025 5293 1632
Size: 800 436 773 1384 268 1917
Fragments arranged by size:
1917 1384 800 773 436 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5381
AflIII A'CryG_T
Cuts at: 1203 2504 4477 5454 1203
Size: 1301 1973 977 1327
Fragments arranged by size:
1973 1327 1301 977
AgeI A'CCGG_T
Cuts at: 4409 4409
Size: 5578
AhdI GACnn_n'nnGTC
Cuts at: 4307 4307
Size: 5578
AleI CACnn'nnGTG
Cuts at: 1166 2507 1166
Size: 1341 4237
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2033 1184
Size: 849 4729
AlwNI CAG_nnn'CTG
Cuts at: 2250 2846 3321 4893 2250
Size: 596 475 1572 2935
Fragments arranged by size:
2935 1572 596 475
ApaLI G'TGCA_C
Cuts at: 2321 3635 4791 2321
Size: 1314 1156 3108
Fragments arranged by size:
3108 1314 1156
ApoI r'AATT_y
Cuts at: 1493 2887 3113 3265 5374 1493
Size: 1394 226 152 2109 1697
Fragments arranged by size:
2109 1697 1394 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4132 160
Size: 872 3100 1606
Fragments arranged by size:
3100 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5578
AvaI C'yCGr_G
Cuts at: 1870 1943 2903 1870
Size: 73 960 4545
Fragments arranged by size:
4545 960 73
BamHI G'GATC_C
Cuts at: 2908 3275 2908
Size: 367 5211
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2899 4354 618
Size: 325 217 627 1112 1455 1842
Fragments arranged by size:
1842 1455 1112 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2279 2357 2379 2778 2897 1391
Size: 888 78 22 399 119 4072
Fragments arranged by size:
4072 888 399 119 78 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2120 2856 928
Size: 971 221 736 3650
Fragments arranged by size:
3650 971 736 221
BbvCI CC'TCA_GC
Cuts at: 2547 2547
Size: 5578
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3769 3803 1903
Size: 34 1832 34 3678
Fragments arranged by size:
3678 1832 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3470 4680 3470
Size: 1210 4368
BclI T'GATC_A
Cuts at: 1548 2283 1548
Size: 735 4843
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4189 136
Size: 107 122 71 631 605 2517 1525
Fragments arranged by size:
2517 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2025 5573 2025
Size: 3548 2030
BlpI GC'TnA_GC
Cuts at: 2995 2995
Size: 5578
Bme1580I G_kGCm'C
Cuts at: 1790 2325 3639 4795 1790
Size: 535 1314 1156 2573
Fragments arranged by size:
2573 1314 1156 535
BmgBI CAC'GTC
Cuts at: 1318 2491 2875 1318
Size: 1173 384 4021
Fragments arranged by size:
4021 1173 384
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3309 4267 442
Size: 416 53 83 399 24 1892 958 1753
Fragments arranged by size:
1892 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5578
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2877 4238 1239
Size: 1638 1361 2579
Fragments arranged by size:
2579 1638 1361
Bpu10I CC'TnA_GC
Cuts at: 2547 2547
Size: 5578
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2675 3703 4568 4866 2675
Size: 1028 865 298 3387
Fragments arranged by size:
3387 1028 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2396 4241 882
Size: 617 897 1845 2219
Fragments arranged by size:
2219 1845 897 617
BsaAI yAC'GTr
Cuts at: 493 1204 3369 493
Size: 711 2165 2702
Fragments arranged by size:
2702 2165 711
BsaBI GATnn'nnATC
Cuts at: 5572 5572
Size: 5578
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2447 3385 3767 4402
Size: 53 83 186 936 914 938 382 635
Cuts at: 4402 275
Size: 1451
Fragments arranged by size:
1451 938 936 914 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2191 2630 4011 4409 4683 4830 5241 2191
Size: 439 1381 398 274 147 411 2528
Fragments arranged by size:
2528 1381 439 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5353
Fragments arranged by size:
5353 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2564 1186
Size: 1378 4200
BseYI C'CCAG_C
Cuts at: 2159 4781 5277 2159
Size: 2622 496 2460
Fragments arranged by size:
2622 2460 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2284 1163
Size: 573 548 4457
Fragments arranged by size:
4457 573 548
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3028 3789 4817 1540
Size: 275 186 1027 761 1028 2301
Fragments arranged by size:
2301 1028 1027 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2325 2357 2379 2477 2532 3639 3724 4795 2325
Size: 32 22 98 55 1107 85 1071 3108
Fragments arranged by size:
3108 1107 1071 98 85 55 32 22
BsmI GAATG_Cn'
Cuts at: 3089 3182 3089
Size: 93 5485
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2279 2325 2357 2379 2477 2532 2778
Size: 399 489 46 32 22 98 55 246
Cuts at: 2778 2897 3639 3724 4795 1391
Size: 119 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 489 399 246 119 98
85 55 46 32 22
BspEI T'CCGG_A
Cuts at: 5241 5241
Size: 5578
BspHI T'CATG_A
Cuts at: 2130 3467 5197 2130
Size: 1337 1730 2511
Fragments arranged by size:
2511 1730 1337
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2933 844
Size: 2089 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3465 1394
Size: 429 1642 3507
Fragments arranged by size:
3507 1642 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2493 4073 4247 65
Size: 1183 1245 1580 174 1396
Fragments arranged by size:
1580 1396 1245 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4222 4409 1700
Size: 110 2412 187 2869
Fragments arranged by size:
2869 2412 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5578
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5578
BssSI C'ACGA_G
Cuts at: 3638 4650 3638
Size: 1012 4566
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2250 794
Size: 1456 4122
BstBI TT'CG_AA
Cuts at: 4382 4382
Size: 5578
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5578
BstYI r'GATC_y
Cuts at: 1972 2025 2247 2908 3275 3660 3677 5129 5215
Size: 53 222 661 367 385 17 1452 86
Cuts at: 5215 5227 5573 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 661 385 367 346 222 86
53 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5026
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4839
Fragments arranged by size:
4839 683 56
BtsI GCAGTG_nn'
Cuts at: 2015 2296 2348 3094 3888 3908 2015
Size: 281 52 746 794 20 3685
Fragments arranged by size:
3685 794 746 281 52 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5578
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5543
DraI TTT'AAA
Cuts at: 2882 2882
Size: 5578
DraIII CAC_nnn'GTG
Cuts at: 2320 2320
Size: 5578
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4585 809
Size: 507 3269 1802
Fragments arranged by size:
3269 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2616 3025 3914
Size: 54 1425 50 275 18 786 409 889
Cuts at: 3914 5543 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 786 409 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3025 1537
Size: 275 1213 4090
Fragments arranged by size:
4090 1213 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2678 2833 3508 1353
Size: 1325 155 675 3423
Fragments arranged by size:
3423 1325 675 155
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4165 4539 4685 387
Size: 1064 138 2576 374 146 1280
Fragments arranged by size:
2576 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2355 2377 2355
Size: 22 5556
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2432 2868 2877 3641 4238 5025 5293
Size: 393 800 436 9 764 597 787 268
Cuts at: 5293 1239
Size: 1524
Fragments arranged by size:
1524 800 787 764 597 436 393 268
9
EcoRI G'AATT_C
Cuts at: 2887 3265 2887
Size: 378 5200
FspI TGC'GCA
Cuts at: 4084 4084
Size: 5578
HaeII r_GCGC'y
Cuts at: 1678 4725 1678
Size: 3047 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2039 2071 2790 2810 2822
Size: 32 135 32 467 32 719 20 12
Cuts at: 2822 2842 2850 2882 4219 4251 4293 4325 1373
Size: 20 8 32 1337 32 42 32 2626
Fragments arranged by size:
2626 1337 719 467 135 42 32 32
32 32 32 32 20 20 12 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2922 3168 669
Size: 988 281 984 246 3079
Fragments arranged by size:
3079 988 984 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5578
HpaI GTT'AAC
Cuts at: 3168 3168
Size: 5578
KpnI G_GTAC'C
Cuts at: 2903 2903
Size: 5578
MfeI C'AATT_G
Cuts at: 2675 3177 2675
Size: 502 5076
MluI A'CGCG_T
Cuts at: 5454 5454
Size: 5578
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2382 2841 4692 4876 1305
Size: 1077 459 1851 184 2007
Fragments arranged by size:
2007 1851 1077 459 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2618 10
Size: 54 1425 343 786 2970
Fragments arranged by size:
2970 1425 786 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2507 2799 3536 3895 4054 518
Size: 648 843 498 292 737 359 159 2042
Fragments arranged by size:
2042 843 737 648 498 359 292 159
MspA1I CmG'CkG
Cuts at: 1606 2994 3671 4819 5064 1606
Size: 1388 677 1148 245 2120
Fragments arranged by size:
2120 1388 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5026
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5578
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5578
NotI GC'GGCC_GC
Cuts at: 3025 3025
Size: 5578
NspI r_CATG'y
Cuts at: 1132 1229 2014 2508 2936 4481 1132
Size: 97 785 494 428 1545 2229
Fragments arranged by size:
2229 1545 785 494 428 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5578
PciI A'CATG_T
Cuts at: 2504 4477 2504
Size: 1973 3605
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5578
PmeI GTTT'AAAC
Cuts at: 2882 2882
Size: 5578
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3647 3679 5160 5192 3647
Size: 32 1481 32 4033
Fragments arranged by size:
4033 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5578
PsiI TTA'TAA
Cuts at: 3148 3148
Size: 5578
PstI C_TGCA'G
Cuts at: 830 2246 2340 2930 830
Size: 1416 94 590 3478
Fragments arranged by size:
3478 1416 590 94
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5578
SacI G_AGCT'C
Cuts at: 2357 2379 2357
Size: 22 5556
SalI G'TCGA_C
Cuts at: 1936 2920 1936
Size: 984 4594
SbfI CC_TGCA'GG
Cuts at: 2930 2930
Size: 5578
ScaI AGT'ACT
Cuts at: 3826 5538 3826
Size: 1712 3866
SfcI C'TryA_G
Cuts at: 826 1045 2068 2242 2336 2926 4061 4742 4933
Size: 219 1023 174 94 590 1135 681 191
Cuts at: 4933 826
Size: 1471
Fragments arranged by size:
1471 1135 1023 681 590 219 191 174
94
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5578
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5578
SmaI CCC'GGG
Cuts at: 1872 2905 1872
Size: 1033 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2690 3682 4583 4845 820
Size: 197 926 747 992 901 262 1553
Fragments arranged by size:
1553 992 926 901 747 262 197
SnaBI TAC'GTA
Cuts at: 493 3369 493
Size: 2876 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5578
SphI G_CATG'C
Cuts at: 1132 2014 2936 1132
Size: 882 922 3774
Fragments arranged by size:
3774 922 882
SspI AAT'ATT
Cuts at: 5 52 2749 3502 5
Size: 47 2697 753 2081
Fragments arranged by size:
2697 2081 753 47
StuI AGG'CCT
Cuts at: 1517 2330 2696 1517
Size: 813 366 4399
Fragments arranged by size:
4399 813 366
StyI C'CwwG_G
Cuts at: 513 1065 3017 513
Size: 552 1952 3074
Fragments arranged by size:
3074 1952 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3605 3622 3775 3960 4405 325
Size: 1255 2025 17 153 185 445 1498
Fragments arranged by size:
2025 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3824 5536 96
Size: 274 80 33 51 1443 1847 1712 138
Fragments arranged by size:
1847 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5538 5570 5538
Size: 32 5546
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5578
XbaI T'CTAG_A
Cuts at: 2914 2914
Size: 5578
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2424 2506 2424
Size: 82 5496
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5578
XmaI C'CCGG_G
Cuts at: 1870 2903 1870
Size: 1033 4545
XmnI GAAnn'nnTTC
Cuts at: 2868 3707 2868
Size: 839 4739
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2448 3386 276
Size: 53 83 186 936 914 938 2468
Fragments arranged by size:
2468 938 936 914 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI