Restriction map for
pFN21AB4815
(Circular) (Six-base) MAPSORT of: pFN21AB4815.seq Check: 9513 from: 1 to: 9127
pFN21AB4815 9127 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3097 3765 4241 3097
Size: 668 476 7983
Fragments arranged by size:
7983 668 476
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6937 278
Size: 53 83 186 936 5401 2468
Fragments arranged by size:
5401 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2499 4111 5970 6470 1937
Size: 562 1612 1859 500 4594
Fragments arranged by size:
4594 1859 1612 562 500
Acc65I G'GTAC_C
Cuts at: 3251 6448 3251
Size: 3197 5930
AclI AA'CG_TT
Cuts at: 1563 7254 7627 1563
Size: 5691 373 3063
Fragments arranged by size:
5691 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2957 3494 3703 4125 4344 4653 4746 4809
Size: 1325 537 209 422 219 309 93 63
Cuts at: 4809 5162 5835 6200 7190 8574 8842 1632
Size: 353 673 365 990 1384 268 1917
Fragments arranged by size:
1917 1384 1325 990 673 537 422 365
353 309 268 219 209 93 63
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8930
AflIII A'CryG_T
Cuts at: 1203 2791 4114 8026 9003 1203
Size: 1588 1323 3912 977 1327
Fragments arranged by size:
3912 1588 1327 1323 977
AgeI A'CCGG_T
Cuts at: 7958 7958
Size: 9127
AhdI GACnn_n'nnGTC
Cuts at: 7856 7856
Size: 9127
AleI CACnn'nnGTG
Cuts at: 1166 5768 1166
Size: 4602 4525
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5748 1184
Size: 4564 4563
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2291 2323 2291
Size: 32 9095
AlwNI CAG_nnn'CTG
Cuts at: 2081 2180 4280 4676 6046 6283 6870 8442 2081
Size: 99 2100 396 1370 237 587 1572 2766
Fragments arranged by size:
2766 2100 1572 1370 587 396 237 99
ApaLI G'TGCA_C
Cuts at: 7184 8340 7184
Size: 1156 7971
ApoI r'AATT_y
Cuts at: 1493 2342 2438 2672 2870 3090 4609 5870 6436
Size: 849 96 234 198 220 1519 1261 566
Cuts at: 6436 6662 6814 8923 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1519 1261 849 566 234 226
220 198 152 96
AseI AT'TA_AT
Cuts at: 160 1032 3954 7681 160
Size: 872 2922 3727 1606
Fragments arranged by size:
3727 2922 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9127
AvaI C'yCGr_G
Cuts at: 1870 1943 4836 5160 6452 1870
Size: 73 2893 324 1292 4545
Fragments arranged by size:
4545 2893 1292 324 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3960 3993 3960
Size: 33 9094
BamHI G'GATC_C
Cuts at: 2509 6457 6824 2509
Size: 3948 367 4812
Fragments arranged by size:
4812 3948 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3251 3458 4663 5575 6448
Size: 325 217 627 1464 207 1205 912 873
Cuts at: 6448 7903 618
Size: 1455 1842
Fragments arranged by size:
1842 1464 1455 1205 912 873 627 325
217 207
BanII G_rGCy'C
Cuts at: 1391 2598 2609 3393 3843 4392 5252 6446 1391
Size: 1207 11 784 450 549 860 1194 4072
Fragments arranged by size:
4072 1207 1194 860 784 549 450 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2277 2920 3564 3930 5150 5408 5841
Size: 971 378 643 644 366 1220 258 433
Cuts at: 5841 928
Size: 4214
Fragments arranged by size:
4214 1220 971 644 643 433 378 366
258
BbvCI CC'TCA_GC
Cuts at: 4260 5616 4260
Size: 1356 7771
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7318 7352 1903
Size: 34 5381 34 3678
Fragments arranged by size:
5381 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7019 8229 7019
Size: 1210 7917
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9127
BfrBI ATG'CAT
Cuts at: 4096 4467 4096
Size: 371 8756
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7738 136
Size: 107 122 71 631 605 6066 1525
Fragments arranged by size:
6066 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6206 6325 6412 9122 6206
Size: 119 87 2710 6211
Fragments arranged by size:
6211 2710 119 87
BlpI GC'TnA_GC
Cuts at: 3837 6544 3837
Size: 2707 6420
Bme1580I G_kGCm'C
Cuts at: 1790 7188 8344 1790
Size: 5398 1156 2573
Fragments arranged by size:
5398 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3655 1318
Size: 2337 6790
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3537 4511 5829
Size: 416 53 83 399 24 2120 974 1318
Cuts at: 5829 6311 6858 7816 442
Size: 482 547 958 1753
Fragments arranged by size:
2120 1753 1318 974 958 547 482 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2255 3957 4886 4980 5354 5408 5960 7787
Size: 1016 1702 929 94 374 54 552 1827
Cuts at: 7787 1239
Size: 2579
Fragments arranged by size:
2579 1827 1702 1016 929 552 374 94
54
Bpu10I CC'TnA_GC
Cuts at: 4260 5616 4260
Size: 1356 7771
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2549 3407 4418 4626 5489 7252 8117 8415 2549
Size: 858 1011 208 863 1763 865 298 3261
Fragments arranged by size:
3261 1763 1011 865 863 858 298 208
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2123 4184 5917 6166 7790 882
Size: 617 624 2061 1733 249 1624 2219
Fragments arranged by size:
2219 2061 1733 1624 624 617 249
BsaAI yAC'GTr
Cuts at: 493 1204 4115 6918 493
Size: 711 2911 2803 2702
Fragments arranged by size:
2911 2803 2702 711
BsaBI GATnn'nnATC
Cuts at: 9121 9121
Size: 9127
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2070 3132 3848 6934
Size: 53 83 186 936 537 1062 716 3086
Cuts at: 6934 7316 7951 275
Size: 382 635 1451
Fragments arranged by size:
3086 1451 1062 936 716 635 537 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 7560 7958 8232 8379 8790 7560
Size: 398 274 147 411 7897
Fragments arranged by size:
7897 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2291 2321 3170 3200 3504
Size: 30 165 30 888 30 849 30 304
Cuts at: 3504 3534 3720 3750 3757 3787 3955 3985 4260
Size: 30 186 30 7 30 168 30 275
Cuts at: 4260 4290 1178
Size: 30 6015
Fragments arranged by size:
6015 888 849 304 275 186 168 165
30 30 30 30 30 30 30 30
30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 2312 3765 5298 5633 5740 1186
Size: 850 276 1453 1533 335 107 4573
Fragments arranged by size:
4573 1533 1453 850 335 276 107
BseYI C'CCAG_C
Cuts at: 3466 3739 5661 8330 8826 3466
Size: 273 1922 2669 496 3767
Fragments arranged by size:
3767 2669 1922 496 273
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4502 5814 6059 1163
Size: 573 2766 1312 245 4231
Fragments arranged by size:
4231 2766 1312 573 245
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6144 6577 7338 8366 1540
Size: 275 186 4143 433 761 1028 2301
Fragments arranged by size:
4143 2301 1028 761 433 275 186
BsiHKAI G_wGCw'C
Cuts at: 2655 3843 4392 5252 5643 7188 7273 8344 2655
Size: 1188 549 860 391 1545 85 1071 3438
Fragments arranged by size:
3438 1545 1188 1071 860 549 391 85
BsiWI C'GTAC_G
Cuts at: 4195 4195
Size: 9127
BsmI GAATG_Cn'
Cuts at: 4034 5737 6638 6731 4034
Size: 1703 901 93 6430
Fragments arranged by size:
6430 1703 901 93
BsmBI CGTCTCn'nnnn_
Cuts at: 6359 6359
Size: 9127
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2598 2609 2655 3393 3843 4392 5252
Size: 399 808 11 46 738 450 549 860
Cuts at: 5252 5643 6446 7188 7273 8344 1391
Size: 391 803 742 85 1071 2174
Fragments arranged by size:
2174 1071 860 808 803 742 738 549
450 399 391 85 46 11
BspEI T'CCGG_A
Cuts at: 8790 8790
Size: 9127
BspHI T'CATG_A
Cuts at: 3400 3822 7016 8746 3400
Size: 422 3194 1730 3781
Fragments arranged by size:
3781 3194 1730 422
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3097 3765 3873 4241 5465 5899 6482 844
Size: 2253 668 108 368 1224 434 583 3489
Fragments arranged by size:
3489 2253 1224 668 583 434 368 108
BsrBI CCG'CTC
Cuts at: 1394 1823 6123 7014 1394
Size: 429 4300 891 3507
Fragments arranged by size:
4300 3507 891 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3603 5377 5915 7622 7796 65
Size: 1183 2355 1774 538 1707 174 1396
Fragments arranged by size:
2355 1774 1707 1396 1183 538 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7771 7958 1700
Size: 110 5961 187 2869
Fragments arranged by size:
5961 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3217 4002 96
Size: 3121 785 5221
Fragments arranged by size:
5221 3121 785
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9127
BssSI C'ACGA_G
Cuts at: 7187 8199 7187
Size: 1012 8115
BstAPI GCAn_nnn'nTGC
Cuts at: 794 6046 794
Size: 5252 3875
BstBI TT'CG_AA
Cuts at: 7931 7931
Size: 9127
BstEII G'GTnAC_C
Cuts at: 1184 5036 5477 1184
Size: 3852 441 4834
Fragments arranged by size:
4834 3852 441
BstXI CCAn_nnnn'nTGG
Cuts at: 2365 3247 3706 2365
Size: 882 459 7786
Fragments arranged by size:
7786 882 459
BstYI r'GATC_y
Cuts at: 1972 2509 2979 4221 6206 6325 6412 6457 6824
Size: 537 470 1242 1985 119 87 45 367
Cuts at: 6824 7209 7226 8678 8764 8776 9122 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1985 1977 1452 1242 537 470 385 367
346 119 87 86 45 17 12
BstZ17I GTA'TAC
Cuts at: 2500 2500
Size: 9127
BtgI C'CryG_G
Cuts at: 513 1065 2004 2454 2664 2839 2893 3198 4440
Size: 552 939 450 210 175 54 305 1242
Cuts at: 4440 5422 513
Size: 982 4218
Fragments arranged by size:
4218 1242 982 939 552 450 305 210
175 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2512 5308 487
Size: 683 56 1286 2796 4306
Fragments arranged by size:
4306 2796 1286 683 56
BtsI GCAGTG_nn'
Cuts at: 3342 4350 4540 5258 5447 6643 7437 7457 3342
Size: 1008 190 718 189 1196 794 20 5012
Fragments arranged by size:
5012 1196 1008 794 718 190 189 20
ClaI AT'CG_AT
Cuts at: 1554 5178 1554
Size: 3624 5503
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9092
DraI TTT'AAA
Cuts at: 6431 6431
Size: 9127
DraIII CAC_nnn'GTG
Cuts at: 4296 4296
Size: 9127
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2075 2387 4107 5543 5845 8134 809
Size: 507 759 312 1720 1436 302 2289 1802
Fragments arranged by size:
2289 1802 1720 1436 759 507 312 302
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3415 3696 6574
Size: 54 1425 50 275 18 1585 281 2878
Cuts at: 6574 7463 9092 8
Size: 889 1629 43
Fragments arranged by size:
2878 1629 1585 1425 889 281 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6574 1537
Size: 275 4762 4090
Fragments arranged by size:
4762 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2228 2730 2933 3324 4141 4170 4351 6009
Size: 875 502 203 391 817 29 181 1658
Cuts at: 6009 7057 1353
Size: 1048 3423
Fragments arranged by size:
3423 1658 1048 875 817 502 391 203
181 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2255 5299 7714 8088 8234 387
Size: 1064 138 666 3044 2415 374 146 1280
Fragments arranged by size:
3044 2415 1280 1064 666 374 146 138
EcoICRI GAG'CTC
Cuts at: 3841 4390 5250 3841
Size: 549 860 7718
Fragments arranged by size:
7718 860 549
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2255 2957 3494 3703 3957 4125 4344
Size: 393 623 702 537 209 254 168 219
Cuts at: 4344 4653 4746 4809 4886 4980 5162 5354 5408
Size: 309 93 63 77 94 182 192 54
Cuts at: 5408 5835 5960 6200 7190 7787 8574 8842 1239
Size: 427 125 240 990 597 787 268 1524
Fragments arranged by size:
1524 990 787 702 623 597 537 427
393 309 268 254 240 219 209 192
182 168 125 94 93 77 63 54
EcoNI CCTnn'n_nnAGG
Cuts at: 6263 6263
Size: 9127
EcoO109I rG'GnC_Cy
Cuts at: 3880 4308 5683 3880
Size: 428 1375 7324
Fragments arranged by size:
7324 1375 428
EcoRI G'AATT_C
Cuts at: 3090 6436 6814 3090
Size: 3346 378 5403
Fragments arranged by size:
5403 3346 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3502 3502
Size: 9127
FspI TGC'GCA
Cuts at: 7633 7633
Size: 9127
HaeII r_GCGC'y
Cuts at: 1678 5560 8274 1678
Size: 3882 2714 2531
Fragments arranged by size:
3882 2714 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3380 3412 3504 3536 4260
Size: 32 135 32 1808 32 92 32 724
Cuts at: 4260 4292 4326 4358 4830 4862 4991 5023 5154
Size: 32 34 32 472 32 129 32 131
Cuts at: 5154 5186 5615 5647 5675 5707 6034 6066 6254
Size: 32 429 32 28 32 327 32 188
Cuts at: 6254 6286 7768 7800 7842 7874 1373
Size: 32 1482 32 42 32 2626
Fragments arranged by size:
2626 1808 1482 724 472 429 327 188
135 131 129 92 42 34 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 28
HincII GTy'rAC
Cuts at: 669 1657 1938 6471 6717 669
Size: 988 281 4533 246 3079
Fragments arranged by size:
4533 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9127
HpaI GTT'AAC
Cuts at: 6717 6717
Size: 9127
KpnI G_GTAC'C
Cuts at: 3255 6452 3255
Size: 3197 5930
MfeI C'AATT_G
Cuts at: 6381 6726 6381
Size: 345 8782
MluI A'CGCG_T
Cuts at: 9003 9003
Size: 9127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2856 6156 8241 8425 1305
Size: 1551 3300 2085 184 2007
Fragments arranged by size:
3300 2085 2007 1551 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3417 3698 10
Size: 54 1425 343 1585 281 5439
Fragments arranged by size:
5439 1585 1425 343 281 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2363 5067 5768 5948 7085 7444 7603
Size: 648 1197 2704 701 180 1137 359 159
Cuts at: 7603 518
Size: 2042
Fragments arranged by size:
2704 2042 1197 1137 701 648 359 180
159
MspA1I CmG'CkG
Cuts at: 1606 2935 3770 4486 6543 7220 8368 8613 1606
Size: 1329 835 716 2057 677 1148 245 2120
Fragments arranged by size:
2120 2057 1329 1148 835 716 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2454 2893 5422 513
Size: 552 939 450 439 2529 4218
Fragments arranged by size:
4218 2529 939 552 450 439
NdeI CA'TA_TG
Cuts at: 387 2770 5207 387
Size: 2383 2437 4307
Fragments arranged by size:
4307 2437 2383
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9127
NotI GC'GGCC_GC
Cuts at: 6574 6574
Size: 9127
NsiI A_TGCA'T
Cuts at: 4098 4469 4098
Size: 371 8756
NspI r_CATG'y
Cuts at: 1132 1229 2795 4172 6485 8030 1132
Size: 97 1566 1377 2313 1545 2229
Fragments arranged by size:
2313 2229 1566 1545 1377 97
PasI CC'CwG_GG
Cuts at: 1348 3896 5737 1348
Size: 2548 1841 4738
Fragments arranged by size:
4738 2548 1841
PciI A'CATG_T
Cuts at: 2791 8026 2791
Size: 5235 3892
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4775 1108
Size: 3667 5460
PmeI GTTT'AAAC
Cuts at: 6431 6431
Size: 9127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2291 2323 3517 3549 7196 7228 8709 8741 2291
Size: 32 1194 32 3647 32 1481 32 2677
Fragments arranged by size:
3647 2677 1481 1194 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3880 5683 3880
Size: 1803 7324
PshAI GACnn'nnGTC
Cuts at: 1613 6230 1613
Size: 4617 4510
PsiI TTA'TAA
Cuts at: 6697 6697
Size: 9127
PspXI vC'TCGA_Gb
Cuts at: 5160 5160
Size: 9127
PstI C_TGCA'G
Cuts at: 830 5907 6479 830
Size: 5077 572 3478
Fragments arranged by size:
5077 3478 572
PvuI CG_AT'CG
Cuts at: 2001 6144 2001
Size: 4143 4984
PvuII CAG'CTG
Cuts at: 2935 4486 2935
Size: 1551 7576
SacI G_AGCT'C
Cuts at: 3843 4392 5252 3843
Size: 549 860 7718
Fragments arranged by size:
7718 860 549
SalI G'TCGA_C
Cuts at: 1936 6469 1936
Size: 4533 4594
SapI GCTCTTCn'nnn_
Cuts at: 4170 4170
Size: 9127
SbfI CC_TGCA'GG
Cuts at: 6479 6479
Size: 9127
ScaI AGT'ACT
Cuts at: 4205 5502 7375 9087 4205
Size: 1297 1873 1712 4245
Fragments arranged by size:
4245 1873 1712 1297
SfcI C'TryA_G
Cuts at: 826 1045 5077 5903 6475 7610 8291 8482 826
Size: 219 4032 826 572 1135 681 191 1471
Fragments arranged by size:
4032 1471 1135 826 681 572 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9127
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9127
SmaI CCC'GGG
Cuts at: 1872 6454 1872
Size: 4582 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2564 3386 4433 4641 5160 5504
Size: 197 926 621 822 1047 208 519 344
Cuts at: 5504 7231 8132 8394 820
Size: 1727 901 262 1553
Fragments arranged by size:
1727 1553 1047 926 901 822 621 519
344 262 208 197
SnaBI TAC'GTA
Cuts at: 493 6918 493
Size: 6425 2702
SpeI A'CTAG_T
Cuts at: 152 2494 152
Size: 2342 6785
SphI G_CATG'C
Cuts at: 1132 6485 1132
Size: 5353 3774
SspI AAT'ATT
Cuts at: 5 52 5998 7051 5
Size: 47 5946 1053 2081
Fragments arranged by size:
5946 2081 1053 47
StuI AGG'CCT
Cuts at: 1517 4424 1517
Size: 2907 6220
StyI C'CwwG_G
Cuts at: 513 1065 2004 2193 2454 2893 2922 5010 5422
Size: 552 939 189 261 439 29 2088 412
Cuts at: 5422 5571 6566 513
Size: 149 995 3074
Fragments arranged by size:
3074 2088 995 939 552 439 412 261
189 149 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3301 5771 7154 7171 7324 7509 7954
Size: 1255 1721 2470 1383 17 153 185 445
Cuts at: 7954 325
Size: 1498
Fragments arranged by size:
2470 1721 1498 1383 1255 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2695 3217 3983
Size: 274 80 33 51 1443 718 522 766
Cuts at: 3983 4002 4203 4960 5500 6390 7373 9085 96
Size: 19 201 757 540 890 983 1712 138
Fragments arranged by size:
1712 1443 983 890 766 757 718 540
522 274 201 138 80 51 33 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2985 3017 3531 3563 3720 3752 3757 3789 3955
Size: 32 514 32 157 32 5 32 166
Cuts at: 3955 3987 9087 9119 2985
Size: 32 5100 32 2993
Fragments arranged by size:
5100 2993 514 166 157 32 32 32
32 32 32 5
Tth111I GACn'n_nGTC
Cuts at: 1316 4697 1316
Size: 3381 5746
XbaI T'CTAG_A
Cuts at: 6463 6463
Size: 9127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2555 3244 4910 5329 5449 5578 2555
Size: 689 1666 419 120 129 6104
Fragments arranged by size:
6104 1666 689 419 129 120
XhoI C'TCGA_G
Cuts at: 1943 5160 1943
Size: 3217 5910
XmaI C'CCGG_G
Cuts at: 1870 6452 1870
Size: 4582 4545
XmnI GAAnn'nnTTC
Cuts at: 2466 2676 7256 2466
Size: 210 4580 4337
Fragments arranged by size:
4580 4337 210
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6935 276
Size: 53 83 186 936 5401 2468
Fragments arranged by size:
5401 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BplI Bsu36I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspOMI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI