Restriction map for
pFN21AB4809
(Circular) (Six-base) MAPSORT of: pFN21AB4809.seq Check: 6840 from: 1 to: 6907
pFN21AB4809 6907 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2298 2298
Size: 6907
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4717 278
Size: 53 83 186 936 3181 2468
Fragments arranged by size:
3181 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3210 4250 1937
Size: 1273 1040 4594
Fragments arranged by size:
4594 1273 1040
Acc65I G'GTAC_C
Cuts at: 2757 3968 4228 2757
Size: 1211 260 5436
Fragments arranged by size:
5436 1211 260
AclI AA'CG_TT
Cuts at: 1563 5034 5407 1563
Size: 3471 373 3063
Fragments arranged by size:
3471 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2444 2705 3197 3701 3770 4970 6354 6622
Size: 812 261 492 504 69 1200 1384 268
Cuts at: 6622 1632
Size: 1917
Fragments arranged by size:
1917 1384 1200 812 504 492 268 261
69
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6710
AflIII A'CryG_T
Cuts at: 1203 3213 5806 6783 1203
Size: 2010 2593 977 1327
Fragments arranged by size:
2593 2010 1327 977
AgeI A'CCGG_T
Cuts at: 5738 5738
Size: 6907
AhdI GACnn_n'nnGTC
Cuts at: 2437 3271 5636 2437
Size: 834 2365 3708
Fragments arranged by size:
3708 2365 834
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6907
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6907
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2951 2983 2951
Size: 32 6875
AlwNI CAG_nnn'CTG
Cuts at: 2299 2683 2698 3175 3737 4650 6222 2299
Size: 384 15 477 562 913 1572 2984
Fragments arranged by size:
2984 1572 913 562 477 384 15
ApaI G_GGCC'C
Cuts at: 3784 3784
Size: 6907
ApaLI G'TGCA_C
Cuts at: 4964 6120 4964
Size: 1156 5751
ApoI r'AATT_y
Cuts at: 1493 3280 4216 4442 4594 6703 1493
Size: 1787 936 226 152 2109 1697
Fragments arranged by size:
2109 1787 1697 936 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5461 160
Size: 872 4429 1606
Fragments arranged by size:
4429 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6907
AvaI C'yCGr_G
Cuts at: 1870 1943 2567 4232 1870
Size: 73 624 1665 4545
Fragments arranged by size:
4545 1665 624 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2749 2782 2749
Size: 33 6874
BamHI G'GATC_C
Cuts at: 4237 4604 4237
Size: 367 6540
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2138 2613 2757 3968 4228
Size: 325 217 627 351 475 144 1211 260
Cuts at: 4228 5683 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1211 627 475 351 325 260
217 144
BanII G_rGCy'C
Cuts at: 1391 2951 3086 3784 4226 1391
Size: 1560 135 698 442 4072
Fragments arranged by size:
4072 1560 698 442 135
BbeI G_GCGC'C
Cuts at: 2617 2617
Size: 6907
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5936
BbvCI CC'TCA_GC
Cuts at: 3872 3872
Size: 6907
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2452 2486 2558 2592 5098 5132 1903
Size: 34 515 34 72 34 2506 34 3678
Fragments arranged by size:
3678 2506 515 72 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4799 6009 4799
Size: 1210 5697
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6907
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2571 3905 5518
Size: 107 122 71 631 605 899 1334 1613
Cuts at: 5518 136
Size: 1525
Fragments arranged by size:
1613 1525 1334 899 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6902 6902
Size: 6907
BlpI GC'TnA_GC
Cuts at: 4019 4324 4019
Size: 305 6602
Bme1580I G_kGCm'C
Cuts at: 1790 2141 3044 3784 4968 6124 1790
Size: 351 903 740 1184 1156 2573
Fragments arranged by size:
2573 1184 1156 903 740 351
BmgBI CAC'GTC
Cuts at: 1318 3216 1318
Size: 1898 5009
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4638 5596 442
Size: 416 53 83 399 24 3221 958 1753
Fragments arranged by size:
3221 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6907
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3025 3025
Size: 6907
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2321 2351 3799 3863 5567 1239
Size: 1082 30 1448 64 1704 2579
Fragments arranged by size:
2579 1704 1448 1082 64 30
Bpu10I CC'TnA_GC
Cuts at: 3872 3872
Size: 6907
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2165 2372 4025 4187 5032 5897 6195 2165
Size: 207 1653 162 845 865 298 2877
Fragments arranged by size:
2877 1653 865 845 298 207 162
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5570 882
Size: 617 4071 2219
Fragments arranged by size:
4071 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3625 4698 493
Size: 711 2421 1073 2702
Fragments arranged by size:
2702 2421 1073 711
BsaBI GATnn'nnATC
Cuts at: 3557 6901 3557
Size: 3344 3563
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2058 2397 2614 4714
Size: 53 83 186 936 525 339 217 2100
Cuts at: 4714 5096 5731 275
Size: 382 635 1451
Fragments arranged by size:
2100 1451 936 635 525 382 339 217
186 83 53
BsaWI w'CCGG_w
Cuts at: 3965 5340 5738 6012 6159 6570 3965
Size: 1375 398 274 147 411 4302
Fragments arranged by size:
4302 1375 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2951 2981 3291 3321 3797
Size: 30 165 30 1548 30 310 30 476
Cuts at: 3797 3827 1178
Size: 30 4258
Fragments arranged by size:
4258 1548 476 310 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2372 2972 2975 2978 3135 3596 3932 1186
Size: 1186 600 3 3 157 461 336 4161
Fragments arranged by size:
4161 1186 600 461 336 157 3 3
BseYI C'CCAG_C
Cuts at: 2609 2936 3168 3419 3486 6110 6606 2609
Size: 327 232 251 67 2624 496 2910
Fragments arranged by size:
2910 2624 496 327 251 232 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3605 1163
Size: 573 1869 4465
Fragments arranged by size:
4465 1869 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2146 3484 4357 5118 6146 1540
Size: 275 186 145 1338 873 761 1028 2301
Fragments arranged by size:
2301 1338 1028 873 761 275 186 145
BsiHKAI G_wGCw'C
Cuts at: 2289 2367 3576 4080 4122 4968 5053 6124 2289
Size: 78 1209 504 42 846 85 1071 3072
Fragments arranged by size:
3072 1209 1071 846 504 85 78 42
BsmI GAATG_Cn'
Cuts at: 2412 4418 4511 2412
Size: 2006 93 4808
Fragments arranged by size:
4808 2006 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3488 3488
Size: 6907
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2141 2289 2367 2951 3044 3086 3576
Size: 399 351 148 78 584 93 42 490
Cuts at: 3576 3784 4080 4122 4226 4968 5053 6124 1391
Size: 208 296 42 104 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 584 490 399 351 296
208 148 104 93 85 78 42 42
BspEI T'CCGG_A
Cuts at: 6570 6570
Size: 6907
BspHI T'CATG_A
Cuts at: 4796 6526 4796
Size: 1730 5177
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2298 3141 4262 844
Size: 1454 843 1121 3489
Fragments arranged by size:
3489 1454 1121 843
BsrBI CCG'CTC
Cuts at: 1394 1823 3777 3813 4794 1394
Size: 429 1954 36 981 3507
Fragments arranged by size:
3507 1954 981 429 36
BsrDI GCAATG_nn'
Cuts at: 65 1248 2547 3060 5402 5576 65
Size: 1183 1299 513 2342 174 1396
Fragments arranged by size:
2342 1396 1299 1183 513 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5551 5738 1700
Size: 110 3741 187 2869
Fragments arranged by size:
3741 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2268 96
Size: 2172 4735
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6907
BssSI C'ACGA_G
Cuts at: 3791 4967 5979 3791
Size: 1176 1012 4719
Fragments arranged by size:
4719 1176 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2698 3737 4147 4177 794
Size: 1904 1039 410 30 3524
Fragments arranged by size:
3524 1904 1039 410 30
BstBI TT'CG_AA
Cuts at: 5711 5711
Size: 6907
BstEII G'GTnAC_C
Cuts at: 1184 2332 2791 1184
Size: 1148 459 5300
Fragments arranged by size:
5300 1148 459
BstXI CCAn_nnnn'nTGG
Cuts at: 3310 3310
Size: 6907
BstYI r'GATC_y
Cuts at: 1972 2230 2454 4164 4237 4604 4989 5006 6458
Size: 258 224 1710 73 367 385 17 1452
Cuts at: 6458 6544 6556 6902 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1710 1452 385 367 346 258 224
86 73 17 12
BstZ17I GTA'TAC
Cuts at: 3211 3211
Size: 6907
BtgI C'CryG_G
Cuts at: 513 1065 2716 3303 3387 513
Size: 552 1651 587 84 4033
Fragments arranged by size:
4033 1651 587 552 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2455 3004 3844 4157 487
Size: 683 56 1229 549 840 313 3237
Fragments arranged by size:
3237 1229 840 683 549 313 56
BtsI GCAGTG_nn'
Cuts at: 4423 5217 5237 4423
Size: 794 20 6093
Fragments arranged by size:
6093 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6907
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6872
DraI TTT'AAA
Cuts at: 4211 4211
Size: 6907
DraIII CAC_nnn'GTG
Cuts at: 2449 4135 2449
Size: 1686 5221
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2378 2774 3163 5914 809
Size: 507 1062 396 389 2751 1802
Fragments arranged by size:
2751 1802 1062 507 396 389
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2235 2256 2923
Size: 54 1425 50 275 18 405 21 667
Cuts at: 2923 3312 3481 3600 3672 4354 5243 6872 8
Size: 389 169 119 72 682 889 1629 43
Fragments arranged by size:
1629 1425 889 682 667 405 389 275
169 119 72 54 50 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 3481 4354 1537
Size: 275 1669 873 4090
Fragments arranged by size:
4090 1669 873 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3938 4837 1353
Size: 2585 899 3423
Fragments arranged by size:
3423 2585 899
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3312 5494 5868 6014 387
Size: 1064 138 1723 2182 374 146 1280
Fragments arranged by size:
2182 1723 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2321 2351 2444 2705 3197 3701 3770
Size: 393 689 30 93 261 492 504 69
Cuts at: 3770 3799 3863 4970 5567 6354 6622 1239
Size: 29 64 1107 597 787 268 1524
Fragments arranged by size:
1524 1107 787 689 597 504 492 393
268 261 93 69 64 30 29
EcoO109I rG'GnC_Cy
Cuts at: 2251 3148 3265 3780 3922 2251
Size: 897 117 515 142 5236
Fragments arranged by size:
5236 897 515 142 117
EcoRI G'AATT_C
Cuts at: 4216 4594 4216
Size: 378 6529
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4066 4066
Size: 6907
FspI TGC'GCA
Cuts at: 3857 5413 3857
Size: 1556 5351
FspAI rTGC'GCAy
Cuts at: 3857 3857
Size: 6907
HaeII r_GCGC'y
Cuts at: 1678 2107 2617 4048 6054 1678
Size: 429 510 1431 2006 2531
Fragments arranged by size:
2531 2006 1431 510 429
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2215 2247 2423 2455 3111
Size: 32 135 32 643 32 176 32 656
Cuts at: 3111 3143 3257 3289 3291 3323 3797 3829 4156
Size: 32 114 32 2 32 474 32 327
Cuts at: 4156 4188 5548 5580 5622 5654 1373
Size: 32 1360 32 42 32 2626
Fragments arranged by size:
2626 1360 656 643 474 327 176 135
114 42 32 32 32 32 32 32
32 32 32 32 32 2
HincII GTy'rAC
Cuts at: 669 1657 1938 4251 4497 669
Size: 988 281 2313 246 3079
Fragments arranged by size:
3079 2313 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6907
HpaI GTT'AAC
Cuts at: 4497 4497
Size: 6907
KasI G'GCGC_C
Cuts at: 2613 2613
Size: 6907
KpnI G_GTAC'C
Cuts at: 2761 3972 4232 2761
Size: 1211 260 5436
Fragments arranged by size:
5436 1211 260
MfeI C'AATT_G
Cuts at: 4506 4506
Size: 6907
MluI A'CGCG_T
Cuts at: 6783 6783
Size: 6907
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2373 2409 3930 6021 6205 1305
Size: 1068 36 1521 2091 184 2007
Fragments arranged by size:
2091 2007 1521 1068 184 36
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2237 3602 3674 10
Size: 54 1425 343 405 1365 72 3243
Fragments arranged by size:
3243 1425 1365 405 343 72 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2552 2903 3047 3767 3830 4865 5224
Size: 648 1386 351 144 720 63 1035 359
Cuts at: 5224 5383 518
Size: 159 2042
Fragments arranged by size:
2042 1386 1035 720 648 359 351 159
144 63
MspA1I CmG'CkG
Cuts at: 1606 2095 2296 2526 3239 3447 3486 3841 4323
Size: 489 201 230 713 208 39 355 482
Cuts at: 4323 5000 6148 6393 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 713 677 489 482 355 245
230 208 201 39
NarI GG'CG_CC
Cuts at: 2614 2614
Size: 6907
NcoI C'CATG_G
Cuts at: 513 1065 3303 3387 513
Size: 552 2238 84 4033
Fragments arranged by size:
4033 2238 552 84
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6907
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6907
NotI GC'GGCC_GC
Cuts at: 4354 4354
Size: 6907
NspI r_CATG'y
Cuts at: 1132 1229 2740 3611 4180 4265 5810 1132
Size: 97 1511 871 569 85 1545 2229
Fragments arranged by size:
2229 1545 1511 871 569 97 85
PasI CC'CwG_GG
Cuts at: 1348 2780 3152 1348
Size: 1432 372 5103
Fragments arranged by size:
5103 1432 372
PciI A'CATG_T
Cuts at: 5806 5806
Size: 6907
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6907
PfoI T'CCnGG_A
Cuts at: 2367 3275 2367
Size: 908 5999
PmeI GTTT'AAAC
Cuts at: 4211 4211
Size: 6907
PmlI CAC'GTG
Cuts at: 3625 3625
Size: 6907
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2951 2983 4976 5008 6489 6521 2951
Size: 32 1993 32 1481 32 3337
Fragments arranged by size:
3337 1993 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2251 3148 3265 2251
Size: 897 117 5893
Fragments arranged by size:
5893 897 117
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6907
PsiI TTA'TAA
Cuts at: 4477 4477
Size: 6907
PspOMI G'GGCC_C
Cuts at: 3780 3780
Size: 6907
PspXI vC'TCGA_Gb
Cuts at: 2567 2567
Size: 6907
PstI C_TGCA'G
Cuts at: 830 2100 2295 2694 3742 3891 4143 4259 830
Size: 1270 195 399 1048 149 252 116 3478
Fragments arranged by size:
3478 1270 1048 399 252 195 149 116
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6907
PvuII CAG'CTG
Cuts at: 2095 2296 2526 3239 3841 2095
Size: 201 230 713 602 5161
Fragments arranged by size:
5161 713 602 230 201
RsrII CG'GwC_CG
Cuts at: 2485 2485
Size: 6907
SalI G'TCGA_C
Cuts at: 1936 4249 1936
Size: 2313 4594
SanDI GG'GwC_CC
Cuts at: 3148 3265 3148
Size: 117 6790
SapI GCTCTTCn'nnn_
Cuts at: 3938 3938
Size: 6907
SbfI CC_TGCA'GG
Cuts at: 4259 4259
Size: 6907
ScaI AGT'ACT
Cuts at: 2207 5155 6867 2207
Size: 2948 1712 2247
Fragments arranged by size:
2948 2247 1712
SexAI A'CCwGG_T
Cuts at: 2445 2445
Size: 6907
SfcI C'TryA_G
Cuts at: 826 1045 2035 2096 2291 2690 3738 3887 4139
Size: 219 990 61 195 399 1048 149 252
Cuts at: 4139 4255 5390 6071 6262 826
Size: 116 1135 681 191 1471
Fragments arranged by size:
1471 1135 1048 990 681 399 252 219
195 191 149 116 61
SfoI GGC'GCC
Cuts at: 2615 2615
Size: 6907
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6907
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6907
SmaI CCC'GGG
Cuts at: 1872 4234 1872
Size: 2362 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2180 2387 2567 4040 4202 5011
Size: 197 926 237 207 180 1473 162 809
Cuts at: 5011 5912 6174 820
Size: 901 262 1553
Fragments arranged by size:
1553 1473 926 901 809 262 237 207
197 180 162
SnaBI TAC'GTA
Cuts at: 493 4698 493
Size: 4205 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6907
SphI G_CATG'C
Cuts at: 1132 4180 4265 1132
Size: 3048 85 3774
Fragments arranged by size:
3774 3048 85
SspI AAT'ATT
Cuts at: 5 52 4831 5
Size: 47 4779 2081
Fragments arranged by size:
4779 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6907
StyI C'CwwG_G
Cuts at: 513 1065 2313 2493 3303 3387 3414 4346 513
Size: 552 1248 180 810 84 27 932 3074
Fragments arranged by size:
3074 1248 932 810 552 180 84 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4934 4951 5104 5289 5734 325
Size: 1255 3354 17 153 185 445 1498
Fragments arranged by size:
3354 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2205 2268 5153
Size: 274 80 33 51 1443 228 63 2885
Cuts at: 5153 6865 96
Size: 1712 138
Fragments arranged by size:
2885 1712 1443 274 228 138 80 63
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6867 6899 6867
Size: 32 6875
Tth111I GACn'n_nGTC
Cuts at: 1316 2771 1316
Size: 1455 5452
XbaI T'CTAG_A
Cuts at: 4243 4243
Size: 6907
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2626 2626
Size: 6907
XhoI C'TCGA_G
Cuts at: 1943 2567 1943
Size: 624 6283
XmaI C'CCGG_G
Cuts at: 1870 4232 1870
Size: 2362 4545
XmnI GAAnn'nnTTC
Cuts at: 5036 5036
Size: 6907
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4715 276
Size: 53 83 186 936 3181 2468
Fragments arranged by size:
3181 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII RsrII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BfrBI BsiWI Bsu36I EcoICRI EcoNI
EcoRV FseI NaeI NgoMIV NruI NsiI PacI PsrI
SacI SacII SfiI SrfI SwaI