Restriction map for
pFN21AB4806
(Circular) (Six-base) MAPSORT of: pFN21AB4806.seq Check: 7602 from: 1 to: 8116
pFN21AB4806 8116 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3259 3259
Size: 8116
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5926 278
Size: 53 83 186 936 4390 2468
Fragments arranged by size:
4390 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3232 5459 1937
Size: 1295 2227 4594
Fragments arranged by size:
4594 2227 1295
Acc65I G'GTAC_C
Cuts at: 2172 2471 5437 2172
Size: 299 2966 4851
Fragments arranged by size:
4851 2966 299
AclI AA'CG_TT
Cuts at: 1563 6243 6616 1563
Size: 4680 373 3063
Fragments arranged by size:
4680 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2900 4049 4901 6179 7563 7831 1632
Size: 1268 1149 852 1278 1384 268 1917
Fragments arranged by size:
1917 1384 1278 1268 1149 852 268
AfeI AGC'GCT
Cuts at: 3004 3004
Size: 8116
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7919
AflIII A'CryG_T
Cuts at: 1203 4959 7015 7992 1203
Size: 3756 2056 977 1327
Fragments arranged by size:
3756 2056 1327 977
AgeI A'CCGG_T
Cuts at: 2468 6947 2468
Size: 4479 3637
AhdI GACnn_n'nnGTC
Cuts at: 6845 6845
Size: 8116
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8116
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8116
AlwNI CAG_nnn'CTG
Cuts at: 2438 2723 2878 2922 3124 3385 3556 3661 4006
Size: 285 155 44 202 261 171 105 345
Cuts at: 4006 4565 5002 5345 5859 7431 2438
Size: 559 437 343 514 1572 3123
Fragments arranged by size:
3123 1572 559 514 437 345 343 285
261 202 171 155 105 44
ApaI G_GGCC'C
Cuts at: 3053 3835 4374 3053
Size: 782 539 6795
Fragments arranged by size:
6795 782 539
ApaLI G'TGCA_C
Cuts at: 6173 7329 6173
Size: 1156 6960
ApoI r'AATT_y
Cuts at: 1493 2026 5425 5651 5803 7912 1493
Size: 533 3399 226 152 2109 1697
Fragments arranged by size:
3399 2109 1697 533 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6670 160
Size: 872 5638 1606
Fragments arranged by size:
5638 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8116
AvaI C'yCGr_G
Cuts at: 1870 1943 2067 2193 2425 2445 3089 5441 1870
Size: 73 124 126 232 20 644 2352 4545
Fragments arranged by size:
4545 2352 644 232 126 124 73 20
AvrII C'CTAG_G
Cuts at: 4970 4970
Size: 8116
BamHI G'GATC_C
Cuts at: 2856 3437 5446 5813 2856
Size: 581 2009 367 5159
Fragments arranged by size:
5159 2009 581 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2172 2214 2471 2632 5437
Size: 325 217 627 385 42 257 161 2805
Cuts at: 5437 6892 618
Size: 1455 1842
Fragments arranged by size:
2805 1842 1455 627 385 325 257 217
161 42
BanII G_rGCy'C
Cuts at: 1391 2576 2799 2840 2904 2969 3053 3835 4374
Size: 1185 223 41 64 65 84 782 539
Cuts at: 4374 5435 1391
Size: 1061 4072
Fragments arranged by size:
4072 1185 1061 782 539 223 84 65
64 41
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2957 3795 4538 4553 4962 928
Size: 971 1058 838 743 15 409 4082
Fragments arranged by size:
4082 1058 971 838 743 409 15
BbvCI CC'TCA_GC
Cuts at: 2161 3797 2161
Size: 1636 6480
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6307 6341 1903
Size: 34 4370 34 3678
Fragments arranged by size:
4370 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6008 7218 6008
Size: 1210 6906
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8116
BfrBI ATG'CAT
Cuts at: 3368 3368
Size: 8116
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2448 4410 6727
Size: 107 122 71 631 605 776 1962 2317
Cuts at: 6727 136
Size: 1525
Fragments arranged by size:
2317 1962 1525 776 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3484 8111 3484
Size: 4627 3489
BlpI GC'TnA_GC
Cuts at: 2839 2849 3750 5533 2839
Size: 10 901 1783 5422
Fragments arranged by size:
5422 1783 901 10
Bme1580I G_kGCm'C
Cuts at: 1790 3053 3835 4374 5124 6177 7333 1790
Size: 1263 782 539 750 1053 1156 2573
Fragments arranged by size:
2573 1263 1156 1053 782 750 539
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8116
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3216 4228 4320
Size: 416 53 83 399 24 1799 1012 92
Cuts at: 4320 5847 6805 442
Size: 1527 958 1753
Fragments arranged by size:
1799 1753 1527 1012 958 416 399 92
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8116
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2103 2759 3988 5041 2103
Size: 656 1229 1053 5178
Fragments arranged by size:
5178 1229 1053 656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2091 2170 2792 3362 3760 4076 4082 4091
Size: 852 79 622 570 398 316 6 9
Cuts at: 4091 4631 4709 4717 4846 5012 5024 6776 1239
Size: 540 78 8 129 166 12 1752 2579
Fragments arranged by size:
2579 1752 852 622 570 540 398 316
166 129 79 78 12 9 8 6
Bpu10I CC'TnA_GC
Cuts at: 2161 3797 2161
Size: 1636 6480
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2189 2229 4103 4588 5243 6241 7106 7404 2189
Size: 40 1874 485 655 998 865 298 2901
Fragments arranged by size:
2901 1874 998 865 655 485 298 40
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2091 3295 4283 5105 6779 882
Size: 617 592 1204 988 822 1674 2219
Fragments arranged by size:
2219 1674 1204 988 822 617 592
BsaAI yAC'GTr
Cuts at: 493 1204 4618 5907 493
Size: 711 3414 1289 2702
Fragments arranged by size:
3414 2702 1289 711
BsaBI GATnn'nnATC
Cuts at: 4820 8110 4820
Size: 3290 4826
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2340 2451 2534 5923
Size: 53 83 186 936 807 111 83 3389
Cuts at: 5923 6305 6940 275
Size: 382 635 1451
Fragments arranged by size:
3389 1451 936 807 635 382 186 111
83 83 53
BsaWI w'CCGG_w
Cuts at: 2468 2679 5097 6549 6947 7221 7368 7779 2468
Size: 211 2418 1452 398 274 147 411 2805
Fragments arranged by size:
2805 2418 1452 411 398 274 211 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2174 2204 4757 4787 5030
Size: 30 165 30 771 30 2553 30 243
Cuts at: 5030 5060 1178
Size: 30 4234
Fragments arranged by size:
4234 2553 771 243 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2192 2741 2744 2990 4913 1186
Size: 1006 549 3 246 1923 4389
Fragments arranged by size:
4389 1923 1006 549 246 3
BseYI C'CCAG_C
Cuts at: 2177 2221 2609 2624 2782 2798 2907 2915 3117
Size: 44 388 15 158 16 109 8 202
Cuts at: 3117 3280 3549 4040 4519 7319 7815 2177
Size: 163 269 491 479 2800 496 2478
Fragments arranged by size:
2800 2478 496 491 479 388 269 202
163 158 109 44 16 15 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7543
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5566 6327 7355 1540
Size: 275 186 3565 761 1028 2301
Fragments arranged by size:
3565 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2202 2417 2840 4617 6177 6262 7333 2202
Size: 215 423 1777 1560 85 1071 2985
Fragments arranged by size:
2985 1777 1560 1071 423 215 85
BsmI GAATG_Cn'
Cuts at: 3840 5627 5720 3840
Size: 1787 93 6236
Fragments arranged by size:
6236 1787 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2331 2331
Size: 8116
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2202 2417 2576 2799 2840 2904 2969
Size: 399 412 215 159 223 41 64 65
Cuts at: 2969 3053 3835 4374 4617 5124 5435 6177 6262
Size: 84 782 539 243 507 311 742 85
Cuts at: 6262 7333 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 782 742 539 507 412 399
311 243 223 215 159 85 84 65
64 41
BspEI T'CCGG_A
Cuts at: 2679 7779 2679
Size: 5100 3016
BspHI T'CATG_A
Cuts at: 4491 6005 7735 4491
Size: 1514 1730 4872
Fragments arranged by size:
4872 1730 1514
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2073 2160 3259 4649 5471 844
Size: 1229 87 1099 1390 822 3489
Fragments arranged by size:
3489 1390 1229 1099 822 87
BsrBI CCG'CTC
Cuts at: 1394 1823 2143 4084 6003 1394
Size: 429 320 1941 1919 3507
Fragments arranged by size:
3507 1941 1919 429 320
BsrDI GCAATG_nn'
Cuts at: 65 1248 4576 6611 6785 65
Size: 1183 3328 2035 174 1396
Fragments arranged by size:
3328 2035 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2211 2468 2766 2980 3696 4455 6760
Size: 110 401 257 298 214 716 759 2305
Cuts at: 6760 6947 1700
Size: 187 2869
Fragments arranged by size:
2869 2305 759 716 401 298 257 214
187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8116
BssHII G'CGCG_C
Cuts at: 1925 4033 1925
Size: 2108 6008
BssSI C'ACGA_G
Cuts at: 6176 7188 6176
Size: 1012 7104
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8116
BstBI TT'CG_AA
Cuts at: 6920 6920
Size: 8116
BstEII G'GTnAC_C
Cuts at: 1184 3323 1184
Size: 2139 5977
BstXI CCAn_nnnn'nTGG
Cuts at: 2048 2048
Size: 8116
BstYI r'GATC_y
Cuts at: 1972 2248 2856 3437 3484 4464 4922 5446 5813
Size: 276 608 581 47 980 458 524 367
Cuts at: 5813 6198 6215 7667 7753 7765 8111 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 980 608 581 524 458 385
367 346 276 86 47 17 12
Bsu36I CC'TnA_GG
Cuts at: 2810 3600 2810
Size: 790 7326
BtgI C'CryG_G
Cuts at: 513 1065 2041 4596 513
Size: 552 976 2555 4033
Fragments arranged by size:
4033 2555 976 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4116 487
Size: 683 56 2890 4487
Fragments arranged by size:
4487 2890 683 56
BtsI GCAGTG_nn'
Cuts at: 2850 3079 3673 4291 4679 5632 6426 6446 2850
Size: 229 594 618 388 953 794 20 4520
Fragments arranged by size:
4520 953 794 618 594 388 229 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8116
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3249 3284 529
Size: 35 2685 35 5361
Fragments arranged by size:
5361 2685 35 35
DraI TTT'AAA
Cuts at: 5420 5420
Size: 8116
DraIII CAC_nnn'GTG
Cuts at: 4750 5147 4750
Size: 397 7719
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7123 809
Size: 507 5807 1802
Fragments arranged by size:
5807 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2592 5563 6452
Size: 54 1425 50 275 18 762 2971 889
Cuts at: 6452 8081 8
Size: 1629 43
Fragments arranged by size:
2971 1629 1425 889 762 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5563 1537
Size: 275 3751 4090
Fragments arranged by size:
4090 3751 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3128 3400 3526 3683 3890 6046 1353
Size: 1775 272 126 157 207 2156 3423
Fragments arranged by size:
3423 2156 1775 272 207 157 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5201 6703 7077 7223 387
Size: 1064 138 3612 1502 374 146 1280
Fragments arranged by size:
3612 1502 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2838 2838
Size: 8116
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2091 2170 2792 2900 3362 3760 4049
Size: 393 459 79 622 108 462 398 289
Cuts at: 4049 4076 4082 4091 4631 4709 4717 4846 4901
Size: 27 6 9 540 78 8 129 55
Cuts at: 4901 5012 5024 6179 6776 7563 7831 1239
Size: 111 12 1155 597 787 268 1524
Fragments arranged by size:
1524 1155 787 622 597 540 462 459
398 393 289 268 129 111 108 79
78 55 27 12 9 8 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2809 4442 2809
Size: 1633 6483
EcoO109I rG'GnC_Cy
Cuts at: 2674 2694 2875 3093 4370 4371 4435 5163 5296
Size: 20 181 218 1277 1 64 728 133
Cuts at: 5296 2674
Size: 5494
Fragments arranged by size:
5494 1277 728 218 181 133 64 20
1
EcoRI G'AATT_C
Cuts at: 2026 5425 5803 2026
Size: 3399 378 4339
Fragments arranged by size:
4339 3399 378
EcoRV GAT'ATC
Cuts at: 2508 2508
Size: 8116
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3199 3199
Size: 8116
FspI TGC'GCA
Cuts at: 3665 6622 3665
Size: 2957 5159
HaeII r_GCGC'y
Cuts at: 1678 2744 2805 3006 4006 4351 4456 4696 4907
Size: 1066 61 201 1000 345 105 240 211
Cuts at: 4907 7263 1678
Size: 2356 2531
Fragments arranged by size:
2531 2356 1066 1000 345 240 211 201
105 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2492 2524 3353 3385 3580
Size: 32 135 32 920 32 829 32 195
Cuts at: 3580 3612 3803 3835 4900 4932 5030 5062 6757
Size: 32 191 32 1065 32 98 32 1695
Cuts at: 6757 6789 6831 6863 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1695 1065 920 829 195 191 135
98 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5460 5706 669
Size: 988 281 3522 246 3079
Fragments arranged by size:
3522 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3848 748
Size: 3100 5016
HpaI GTT'AAC
Cuts at: 5706 5706
Size: 8116
KpnI G_GTAC'C
Cuts at: 2176 2475 5441 2176
Size: 299 2966 4851
Fragments arranged by size:
4851 2966 299
MfeI C'AATT_G
Cuts at: 5715 5715
Size: 8116
MluI A'CGCG_T
Cuts at: 7992 7992
Size: 8116
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3001 4963 5064 5073 7230 7414 1305
Size: 1696 1962 101 9 2157 184 2007
Fragments arranged by size:
2157 2007 1962 1696 184 101 9
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6294
Fragments arranged by size:
6294 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3726 4541 4706 6074 6433 6592
Size: 648 843 1717 815 165 1368 359 159
Cuts at: 6592 518
Size: 2042
Fragments arranged by size:
2042 1717 1368 843 815 648 359 165
159
MspA1I CmG'CkG
Cuts at: 1606 2438 2922 3121 3152 3754 4472 4737 5532
Size: 832 484 199 31 602 718 265 795
Cuts at: 5532 6209 7357 7602 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 832 795 718 677 602 484
265 245 199 31
NaeI GCC'GGC
Cuts at: 2982 4457 2982
Size: 1475 6641
NcoI C'CATG_G
Cuts at: 513 1065 4596 513
Size: 552 3531 4033
Fragments arranged by size:
4033 3531 552
NdeI CA'TA_TG
Cuts at: 387 3505 387
Size: 3118 4998
NgoMIV G'CCGG_C
Cuts at: 2980 4455 2980
Size: 1475 6641
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8116
NotI GC'GGCC_GC
Cuts at: 5563 5563
Size: 8116
NruI TCG'CGA
Cuts at: 4135 4135
Size: 8116
NsiI A_TGCA'T
Cuts at: 3370 3370
Size: 8116
NspI r_CATG'y
Cuts at: 1132 1229 2554 3319 3739 5474 7019 1132
Size: 97 1325 765 420 1735 1545 2229
Fragments arranged by size:
2229 1735 1545 1325 765 420 97
PasI CC'CwG_GG
Cuts at: 1348 4526 1348
Size: 3178 4938
PciI A'CATG_T
Cuts at: 7015 7015
Size: 8116
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2438 2488 4260 4565 4750 1108
Size: 1330 50 1772 305 185 4474
Fragments arranged by size:
4474 1772 1330 305 185 50
PfoI T'CCnGG_A
Cuts at: 2496 2751 2496
Size: 255 7861
PmeI GTTT'AAAC
Cuts at: 5420 5420
Size: 8116
PmlI CAC'GTG
Cuts at: 4618 4618
Size: 8116
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6185 6217 7698 7730 6185
Size: 32 1481 32 6571
Fragments arranged by size:
6571 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2694 2875 3093 4435 2694
Size: 181 218 1342 6375
Fragments arranged by size:
6375 1342 218 181
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8116
PsiI TTA'TAA
Cuts at: 5686 5686
Size: 8116
PspOMI G'GGCC_C
Cuts at: 3049 3831 4370 3049
Size: 782 539 6795
Fragments arranged by size:
6795 782 539
PstI C_TGCA'G
Cuts at: 830 2171 2648 4346 5468 830
Size: 1341 477 1698 1122 3478
Fragments arranged by size:
3478 1698 1341 1122 477
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8116
PvuII CAG'CTG
Cuts at: 2438 2922 3754 4737 2438
Size: 484 832 983 5817
Fragments arranged by size:
5817 983 832 484
SacI G_AGCT'C
Cuts at: 2840 2840
Size: 8116
SalI G'TCGA_C
Cuts at: 1936 5458 1936
Size: 3522 4594
SanDI GG'GwC_CC
Cuts at: 3093 3093
Size: 8116
SapI GCTCTTCn'nnn_
Cuts at: 3526 3683 3526
Size: 157 7959
SbfI CC_TGCA'GG
Cuts at: 2171 5468 2171
Size: 3297 4819
ScaI AGT'ACT
Cuts at: 4100 5324 6364 8076 4100
Size: 1224 1040 1712 4140
Fragments arranged by size:
4140 1712 1224 1040
SfcI C'TryA_G
Cuts at: 826 1045 2167 2644 2926 4342 5464 6599 7280
Size: 219 1122 477 282 1416 1122 1135 681
Cuts at: 7280 7471 826
Size: 191 1471
Fragments arranged by size:
1471 1416 1135 1122 1122 681 477 282
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8116
SgrAI Cr'CCGG_yG
Cuts at: 1700 4455 1700
Size: 2755 5361
SmaI CCC'GGG
Cuts at: 1872 2195 2427 2447 3091 5443 1872
Size: 323 232 20 644 2352 4545
Fragments arranged by size:
4545 2352 644 323 232 20
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2204 2244 4118 4603 5222 6220
Size: 197 926 261 40 1874 485 619 998
Cuts at: 6220 7121 7383 820
Size: 901 262 1553
Fragments arranged by size:
1874 1553 998 926 901 619 485 262
261 197 40
SnaBI TAC'GTA
Cuts at: 493 5907 493
Size: 5414 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8116
SphI G_CATG'C
Cuts at: 1132 5474 1132
Size: 4342 3774
SspI AAT'ATT
Cuts at: 5 52 6040 5
Size: 47 5988 2081
Fragments arranged by size:
5988 2081 47
StuI AGG'CCT
Cuts at: 1517 5180 1517
Size: 3663 4453
StyI C'CwwG_G
Cuts at: 513 1065 2123 2359 2961 2970 3492 3570 4254
Size: 552 1058 236 602 9 522 78 684
Cuts at: 4254 4408 4431 4596 4970 5555 513
Size: 154 23 165 374 585 3074
Fragments arranged by size:
3074 1058 684 602 585 552 522 374
236 165 154 78 23 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2054 2287 3259 4197 4944 6143 6160
Size: 1255 474 233 972 938 747 1199 17
Cuts at: 6160 6313 6498 6943 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1199 972 938 747 474 445
233 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3612 4098 4389
Size: 274 80 33 51 1443 1635 486 291
Cuts at: 4389 5322 6362 8074 96
Size: 933 1040 1712 138
Fragments arranged by size:
1712 1635 1443 1040 933 486 291 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2174 2206 3317 3349 4613 4645 4777 4809 8076
Size: 32 1111 32 1264 32 132 32 3267
Cuts at: 8076 8108 2174
Size: 32 2182
Fragments arranged by size:
3267 2182 1264 1111 132 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4823 1316
Size: 3507 4609
XbaI T'CTAG_A
Cuts at: 5452 5452
Size: 8116
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2048 3431 4843 2048
Size: 1383 1412 5321
Fragments arranged by size:
5321 1412 1383
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8116
XmaI C'CCGG_G
Cuts at: 1870 2193 2425 2445 3089 5441 1870
Size: 323 232 20 644 2352 4545
Fragments arranged by size:
4545 2352 644 323 232 20
XmnI GAAnn'nnTTC
Cuts at: 2320 4550 6245 2320
Size: 2230 1695 4191
Fragments arranged by size:
4191 2230 1695
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5924 276
Size: 53 83 186 936 4390 2468
Fragments arranged by size:
4390 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BsiWI BstZ17I FseI FspAI
KasI NarI PacI PspXI PsrI RsrII SacII SexAI
SfiI SfoI SrfI SwaI