Restriction map for
pFN21AB4800
(Circular) (Six-base) MAPSORT of: pFN21AB4800.seq Check: 3553 from: 1 to: 5971
pFN21AB4800 5971 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3003 3003
Size: 5971
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3781 278
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3314 1937
Size: 1377 4594
Acc65I G'GTAC_C
Cuts at: 2843 3292 2843
Size: 449 5522
AclI AA'CG_TT
Cuts at: 1563 4098 4471 1563
Size: 2535 373 3063
Fragments arranged by size:
3063 2535 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4034 5418 5686 1632
Size: 2402 1384 268 1917
Fragments arranged by size:
2402 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5774
AflIII A'CryG_T
Cuts at: 1203 4870 5847 1203
Size: 3667 977 1327
Fragments arranged by size:
3667 1327 977
AgeI A'CCGG_T
Cuts at: 4802 4802
Size: 5971
AhdI GACnn_n'nnGTC
Cuts at: 2167 2614 4700 2167
Size: 447 2086 3438
Fragments arranged by size:
3438 2086 447
AleI CACnn'nnGTG
Cuts at: 1166 3093 1166
Size: 1927 4044
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2762 1184
Size: 1578 4393
AlwNI CAG_nnn'CTG
Cuts at: 2241 3714 5286 2241
Size: 1473 1572 2926
Fragments arranged by size:
2926 1572 1473
ApaI G_GGCC'C
Cuts at: 2437 2437
Size: 5971
ApaLI G'TGCA_C
Cuts at: 2737 2819 2918 4028 5184 2737
Size: 82 99 1110 1156 3524
Fragments arranged by size:
3524 1156 1110 99 82
ApoI r'AATT_y
Cuts at: 1493 3280 3506 3658 5767 1493
Size: 1787 226 152 2109 1697
Fragments arranged by size:
2109 1787 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4525 160
Size: 872 3493 1606
Fragments arranged by size:
3493 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
AvaI C'yCGr_G
Cuts at: 1870 1943 2157 2672 2813 3296 1870
Size: 73 214 515 141 483 4545
Fragments arranged by size:
4545 515 483 214 141 73
AvrII C'CTAG_G
Cuts at: 2593 2593
Size: 5971
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2827 2860 2827
Size: 33 5938
BamHI G'GATC_C
Cuts at: 3301 3668 3301
Size: 367 5604
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2104 2403 2545 2573 2843
Size: 325 217 627 317 299 142 28 270
Cuts at: 2843 3243 3292 4747 618
Size: 400 49 1455 1842
Fragments arranged by size:
1842 1455 627 400 325 317 299 270
217 142 49 28
BanII G_rGCy'C
Cuts at: 1391 2157 2255 2437 2601 2814 3146 3267 3290
Size: 766 98 182 164 213 332 121 23
Cuts at: 3290 1391
Size: 4072
Fragments arranged by size:
4072 766 332 213 182 164 121 98
23
BbeI G_GCGC'C
Cuts at: 2108 2549 2108
Size: 441 5530
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5000
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2863 2897 4162 4196 1903
Size: 34 50 34 842 34 1265 34 3678
Fragments arranged by size:
3678 1265 842 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3863 5073 3863
Size: 1210 4761
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5971
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2809 4582 136
Size: 107 122 71 631 605 1137 1773 1525
Fragments arranged by size:
1773 1525 1137 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2949 5966 2949
Size: 3017 2954
BlpI GC'TnA_GC
Cuts at: 3388 3388
Size: 5971
Bme1580I G_kGCm'C
Cuts at: 1790 2324 2437 2741 2823 2889 2922 4032 5188
Size: 534 113 304 82 66 33 1110 1156
Cuts at: 5188 1790
Size: 2573
Fragments arranged by size:
2573 1156 1110 534 304 113 82 66
33
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5971
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3702 4660 442
Size: 416 53 83 399 24 2285 958 1753
Fragments arranged by size:
2285 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2283 2293 2459 2921 4631 1239
Size: 1044 10 166 462 1710 2579
Fragments arranged by size:
2579 1710 1044 462 166 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4096 4961 5259 4096
Size: 865 298 4808
Fragments arranged by size:
4808 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4634 882
Size: 617 3135 2219
Fragments arranged by size:
3135 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2824 3762 493
Size: 711 1620 938 2702
Fragments arranged by size:
2702 1620 938 711
BsaBI GATnn'nnATC
Cuts at: 5965 5965
Size: 5971
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2105 2546 2712 3778
Size: 53 83 186 936 572 441 166 1066
Cuts at: 3778 4160 4795 275
Size: 382 635 1451
Fragments arranged by size:
1451 1066 936 635 572 441 382 186
166 83 53
BsaWI w'CCGG_w
Cuts at: 4404 4802 5076 5223 5634 4404
Size: 398 274 147 411 4741
Fragments arranged by size:
4741 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2192 2222 2720 2750 1178
Size: 30 165 30 789 30 498 30 4399
Fragments arranged by size:
4399 789 498 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5971
BseYI C'CCAG_C
Cuts at: 2340 2752 5174 5670 2340
Size: 412 2422 496 2641
Fragments arranged by size:
2641 2422 496 412
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2432 2675 2803 1163
Size: 573 696 243 128 4331
Fragments arranged by size:
4331 696 573 243 128
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2426 2499 3421 4182 5210 1540
Size: 275 186 425 73 922 761 1028 2301
Fragments arranged by size:
2301 1028 922 761 425 275 186 73
BsiHKAI G_wGCw'C
Cuts at: 2255 2741 2823 2922 3146 4032 4117 5188 2255
Size: 486 82 99 224 886 85 1071 3038
Fragments arranged by size:
3038 1071 886 486 224 99 85 82
BsmI GAATG_Cn'
Cuts at: 3131 3482 3575 3131
Size: 351 93 5527
Fragments arranged by size:
5527 351 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2301 3245 2301
Size: 944 5027
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2157 2255 2324 2437 2601 2741 2814
Size: 399 367 98 69 113 164 140 73
Cuts at: 2814 2823 2889 2922 3146 3267 3290 4032 4117
Size: 9 66 33 224 121 23 742 85
Cuts at: 4117 5188 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 367 224 164 140
121 113 98 85 73 69 66 33
23 9
BspEI T'CCGG_A
Cuts at: 5634 5634
Size: 5971
BspHI T'CATG_A
Cuts at: 3860 5590 3860
Size: 1730 4241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2406 2820 3003 3326 844
Size: 1562 414 183 323 3489
Fragments arranged by size:
3489 1562 414 323 183
BsrBI CCG'CTC
Cuts at: 1394 1823 2379 3858 1394
Size: 429 556 1479 3507
Fragments arranged by size:
3507 1479 556 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4466 4640 65
Size: 1183 3218 174 1396
Fragments arranged by size:
3218 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2101 2525 2570 4615 4802 1700
Size: 110 291 424 45 2045 187 2869
Fragments arranged by size:
2869 2045 424 291 187 110 45
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5971
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5971
BssSI C'ACGA_G
Cuts at: 4031 5043 4031
Size: 1012 4959
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2241 2410 2781 794
Size: 1447 169 371 3984
Fragments arranged by size:
3984 1447 371 169
BstBI TT'CG_AA
Cuts at: 4775 4775
Size: 5971
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5971
BstYI r'GATC_y
Cuts at: 1972 2949 3301 3668 4053 4070 5522 5608 5620
Size: 977 352 367 385 17 1452 86 12
Cuts at: 5620 5966 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 977 385 367 352 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2646 2683 513
Size: 552 1581 37 3801
Fragments arranged by size:
3801 1581 552 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2310 487
Size: 683 56 1084 4148
Fragments arranged by size:
4148 1084 683 56
BtsI GCAGTG_nn'
Cuts at: 3487 4281 4301 3487
Size: 794 20 5157
Fragments arranged by size:
5157 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5971
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5936
DraI TTT'AAA
Cuts at: 3275 3275
Size: 5971
DraIII CAC_nnn'GTG
Cuts at: 2410 2893 2410
Size: 483 5488
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4978 809
Size: 507 3662 1802
Fragments arranged by size:
3662 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2488 2496 2680
Size: 54 1425 50 275 18 658 8 184
Cuts at: 2680 3418 4307 5936 8
Size: 738 889 1629 43
Fragments arranged by size:
1629 1425 889 738 658 275 184 54
50 43 18 8
EagI C'GGCC_G
Cuts at: 1537 1812 2496 3418 1537
Size: 275 684 922 4090
Fragments arranged by size:
4090 922 684 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2468 2954 3901 1353
Size: 1115 486 947 3423
Fragments arranged by size:
3423 1115 947 486
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2159 2165 4558 4932 5078 387
Size: 1064 138 570 6 2393 374 146 1280
Fragments arranged by size:
2393 1280 1064 570 374 146 138 6
EcoICRI GAG'CTC
Cuts at: 2253 3144 2253
Size: 891 5080
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2283 2293 2459 2921 4034 4631 5418
Size: 393 651 10 166 462 1113 597 787
Cuts at: 5418 5686 1239
Size: 268 1524
Fragments arranged by size:
1524 1113 787 651 597 462 393 268
166 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2592 2592
Size: 5971
EcoO109I rG'GnC_Cy
Cuts at: 2277 2433 2434 2832 2277
Size: 156 1 398 5416
Fragments arranged by size:
5416 398 156 1
EcoRI G'AATT_C
Cuts at: 3280 3658 3280
Size: 378 5593
FspI TGC'GCA
Cuts at: 2828 4477 2828
Size: 1649 4322
HaeII r_GCGC'y
Cuts at: 1678 2108 2451 2549 2709 5118 1678
Size: 430 343 98 160 2409 2531
Fragments arranged by size:
2531 2409 430 343 160 98
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2153 2185 2192 2224 2600
Size: 32 135 32 581 32 7 32 376
Cuts at: 2600 2632 4612 4644 4686 4718 1373
Size: 32 1980 32 42 32 2626
Fragments arranged by size:
2626 1980 581 376 135 42 32 32
32 32 32 32 32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3315 3561 669
Size: 988 281 1377 246 3079
Fragments arranged by size:
3079 1377 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5971
HpaI GTT'AAC
Cuts at: 3561 3561
Size: 5971
KasI G'GCGC_C
Cuts at: 2104 2545 2104
Size: 441 5530
KpnI G_GTAC'C
Cuts at: 2847 3296 2847
Size: 449 5522
MfeI C'AATT_G
Cuts at: 3570 3570
Size: 5971
MluI A'CGCG_T
Cuts at: 5847 5847
Size: 5971
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2706 3171 5085 5269 1305
Size: 1401 465 1914 184 2007
Fragments arranged by size:
2007 1914 1401 465 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2490 2682 10
Size: 54 1425 343 658 192 3299
Fragments arranged by size:
3299 1425 658 343 192 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3093 3929 4288 4447 518
Size: 648 1927 836 359 159 2042
Fragments arranged by size:
2042 1927 836 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2185 2226 2582 2654 2841 3387 4064 5212
Size: 579 41 356 72 187 546 677 1148
Cuts at: 5212 5457 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 579 546 356 245 187
72 41
NaeI GCC'GGC
Cuts at: 2527 2527
Size: 5971
NarI GG'CG_CC
Cuts at: 2105 2546 2105
Size: 441 5530
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5419
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5971
NgoMIV G'CCGG_C
Cuts at: 2525 2525
Size: 5971
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5971
NotI GC'GGCC_GC
Cuts at: 3418 3418
Size: 5971
NspI r_CATG'y
Cuts at: 1132 1229 3329 4874 1132
Size: 97 2100 1545 2229
Fragments arranged by size:
2229 2100 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5971
PciI A'CATG_T
Cuts at: 4870 4870
Size: 5971
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5971
PmeI GTTT'AAAC
Cuts at: 3275 3275
Size: 5971
PmlI CAC'GTG
Cuts at: 2824 2824
Size: 5971
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4040 4072 5553 5585 4040
Size: 32 1481 32 4426
Fragments arranged by size:
4426 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2832 2832
Size: 5971
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5971
PsiI TTA'TAA
Cuts at: 3541 3541
Size: 5971
PspOMI G'GGCC_C
Cuts at: 2433 2433
Size: 5971
PspXI vC'TCGA_Gb
Cuts at: 2813 2813
Size: 5971
PstI C_TGCA'G
Cuts at: 830 2561 3323 830
Size: 1731 762 3478
Fragments arranged by size:
3478 1731 762
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5971
PvuII CAG'CTG
Cuts at: 2185 2226 2185
Size: 41 5930
SacI G_AGCT'C
Cuts at: 2255 3146 2255
Size: 891 5080
SalI G'TCGA_C
Cuts at: 1936 3313 1936
Size: 1377 4594
SapI GCTCTTCn'nnn_
Cuts at: 2954 2954
Size: 5971
SbfI CC_TGCA'GG
Cuts at: 3323 3323
Size: 5971
ScaI AGT'ACT
Cuts at: 3035 4219 5931 3035
Size: 1184 1712 3075
Fragments arranged by size:
3075 1712 1184
SexAI A'CCwGG_T
Cuts at: 2406 2406
Size: 5971
SfcI C'TryA_G
Cuts at: 826 1045 2557 3319 4454 5135 5326 826
Size: 219 1512 762 1135 681 191 1471
Fragments arranged by size:
1512 1471 1135 762 681 219 191
SfoI GGC'GCC
Cuts at: 2106 2547 2106
Size: 441 5530
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5971
SmaI CCC'GGG
Cuts at: 1872 2159 3298 1872
Size: 287 1139 4545
Fragments arranged by size:
4545 1139 287
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2813 4075 4976 5238 820
Size: 197 926 870 1262 901 262 1553
Fragments arranged by size:
1553 1262 926 901 870 262 197
SnaBI TAC'GTA
Cuts at: 493 3762 493
Size: 3269 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5971
SphI G_CATG'C
Cuts at: 1132 3329 1132
Size: 2197 3774
SspI AAT'ATT
Cuts at: 5 52 3895 5
Size: 47 3843 2081
Fragments arranged by size:
3843 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5971
StyI C'CwwG_G
Cuts at: 513 1065 2593 3410 513
Size: 552 1528 817 3074
Fragments arranged by size:
3074 1528 817 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2204 3173 3998 4015 4168 4353 4798
Size: 1255 624 969 825 17 153 185 445
Cuts at: 4798 325
Size: 1498
Fragments arranged by size:
1498 1255 969 825 624 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3033 4217 5929
Size: 274 80 33 51 1443 1056 1184 1712
Cuts at: 5929 96
Size: 138
Fragments arranged by size:
1712 1443 1184 1056 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2720 2752 5931 5963 2720
Size: 32 3179 32 2728
Fragments arranged by size:
3179 2728 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5971
XbaI T'CTAG_A
Cuts at: 3307 3307
Size: 5971
XhoI C'TCGA_G
Cuts at: 1943 2813 1943
Size: 870 5101
XmaI C'CCGG_G
Cuts at: 1870 2157 3296 1870
Size: 287 1139 4545
Fragments arranged by size:
4545 1139 287
XmnI GAAnn'nnTTC
Cuts at: 2963 3215 4100 2963
Size: 252 885 4834
Fragments arranged by size:
4834 885 252
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3779 276
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI BbvCI BfrBI BplI Bpu10I BsiWI
BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PsrI RsrII SacII SanDI SfiI
SrfI SwaI XcmI