Restriction map for
pFN21AB4786
(Circular) (Six-base) MAPSORT of: pFN21AB4786.seq Check: 2890 from: 1 to: 9097
pFN21AB4786 9097 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2129 5786 2129
Size: 3657 5440
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4759 6907 278
Size: 53 83 186 936 3223 2148 2468
Fragments arranged by size:
3223 2468 2148 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6440 1937
Size: 4503 4594
Acc65I G'GTAC_C
Cuts at: 2142 6418 2142
Size: 4276 4821
AclI AA'CG_TT
Cuts at: 1563 7224 7597 1563
Size: 5661 373 3063
Fragments arranged by size:
5661 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2628 3114 4166 5581 7160 8544 8812 1632
Size: 996 486 1052 1415 1579 1384 268 1917
Fragments arranged by size:
1917 1579 1415 1384 1052 996 486 268
AfeI AGC'GCT
Cuts at: 4606 5584 4606
Size: 978 8119
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8900
AflIII A'CryG_T
Cuts at: 1203 2157 7996 8973 1203
Size: 954 5839 977 1327
Fragments arranged by size:
5839 1327 977 954
AgeI A'CCGG_T
Cuts at: 7928 7928
Size: 9097
AhdI GACnn_n'nnGTC
Cuts at: 7826 7826
Size: 9097
AleI CACnn'nnGTG
Cuts at: 1166 5637 1166
Size: 4471 4626
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9097
AlwNI CAG_nnn'CTG
Cuts at: 6840 8412 6840
Size: 1572 7525
ApaI G_GGCC'C
Cuts at: 2912 3003 3084 3272 5009 5496 2912
Size: 91 81 188 1737 487 6513
Fragments arranged by size:
6513 1737 487 188 91 81
ApaLI G'TGCA_C
Cuts at: 2586 7154 8310 2586
Size: 4568 1156 3373
Fragments arranged by size:
4568 3373 1156
ApoI r'AATT_y
Cuts at: 1493 2526 6362 6406 6632 6784 8893 1493
Size: 1033 3836 44 226 152 2109 1697
Fragments arranged by size:
3836 2109 1697 1033 226 152 44
AseI AT'TA_AT
Cuts at: 160 1032 7651 160
Size: 872 6619 1606
Fragments arranged by size:
6619 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9097
AvaI C'yCGr_G
Cuts at: 1870 1943 2168 2264 2361 2661 2760 3084 3246
Size: 73 225 96 97 300 99 324 162
Cuts at: 3246 3417 4092 4850 5424 5690 6422 1870
Size: 171 675 758 574 266 732 4545
Fragments arranged by size:
4545 758 732 675 574 324 300 266
225 171 162 99 97 96 73
BamHI G'GATC_C
Cuts at: 4808 6427 6794 4808
Size: 1619 367 7111
Fragments arranged by size:
7111 1619 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2142 2252 2828 3197 3242
Size: 325 217 627 355 110 576 369 45
Cuts at: 3242 3611 3719 4007 4142 4169 4331 4394 5060
Size: 369 108 288 135 27 162 63 666
Cuts at: 5060 5096 5159 5249 6347 6418 7873 618
Size: 36 63 90 1098 71 1455 1842
Fragments arranged by size:
1842 1455 1098 666 627 576 369 369
355 325 288 217 162 135 110 108
90 71 63 63 45 36 27
BanII G_rGCy'C
Cuts at: 1391 2175 2896 2912 2950 3003 3084 3272 3418
Size: 784 721 16 38 53 81 188 146
Cuts at: 3418 3780 3859 4156 4390 5009 5056 5496 5747
Size: 362 79 297 234 619 47 440 251
Cuts at: 5747 6032 6182 6416 1391
Size: 285 150 234 4072
Fragments arranged by size:
4072 784 721 619 440 362 297 285
251 234 234 188 150 146 81 79
53 47 38 16
BbeI G_GCGC'C
Cuts at: 2256 4011 4173 5100 5253 2256
Size: 1755 162 927 153 6100
Fragments arranged by size:
6100 1755 927 162 153
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2109 4208 4919 5863 928
Size: 971 210 2099 711 944 4162
Fragments arranged by size:
4162 2099 971 944 711 210
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7288 7322 1903
Size: 34 5351 34 3678
Fragments arranged by size:
5351 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6989 8199 6989
Size: 1210 7887
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9097
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2295 2364 2391
Size: 107 122 71 631 605 623 69 27
Cuts at: 2391 2400 2746 2916 3132 3276 3555 3915 4437
Size: 9 346 170 216 144 279 360 522
Cuts at: 4437 4797 5175 5184 5247 5373 5751 6047 7708
Size: 360 378 9 63 126 378 296 1661
Cuts at: 7708 136
Size: 1525
Fragments arranged by size:
1661 1525 631 623 605 522 378 378
360 360 346 296 279 216 170 144
126 122 107 71 69 63 27 9
9
BglII A'GATC_T
Cuts at: 9092 9092
Size: 9097
BlpI GC'TnA_GC
Cuts at: 6514 6514
Size: 9097
Bme1580I G_kGCm'C
Cuts at: 1790 2590 2912 3003 3084 3247 3272 3724 4147
Size: 800 322 91 81 163 25 452 423
Cuts at: 4147 4336 4399 5009 5164 5496 6352 7158 8314
Size: 189 63 610 155 332 856 806 1156
Cuts at: 8314 1790
Size: 2573
Fragments arranged by size:
2573 1156 856 806 800 610 452 423
332 322 189 163 155 91 81 63
25
BmgBI CAC'GTC
Cuts at: 1318 2158 2221 5045 1318
Size: 840 63 2824 5370
Fragments arranged by size:
5370 2824 840 63
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5913 6828 7786
Size: 416 53 83 399 24 4496 915 958
Cuts at: 7786 442
Size: 1753
Fragments arranged by size:
4496 1753 958 915 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2658 2964 3414 3792 3810 3873 3942 4017
Size: 1419 306 450 378 18 63 69 75
Cuts at: 4017 4044 4170 4404 4602 4989 5336 5421 6158
Size: 27 126 234 198 387 347 85 737
Cuts at: 6158 7757 1239
Size: 1599 2579
Fragments arranged by size:
2579 1599 1419 737 450 387 378 347
306 234 198 126 85 75 69 63
27 18
Bpu10I CC'TnA_GC
Cuts at: 3057 3345 3858 4128 5717 3057
Size: 288 513 270 1589 6437
Fragments arranged by size:
6437 1589 513 288 270
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4842 5604 5693 5774 6140 7222 8087 8385 4842
Size: 762 89 81 366 1082 865 298 5554
Fragments arranged by size:
5554 1082 865 762 366 298 89 81
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2228 2573 3941 3993 4398 5111 5480
Size: 617 729 345 1368 52 405 713 369
Cuts at: 5480 5888 7760 882
Size: 408 1872 2219
Fragments arranged by size:
2219 1872 1368 729 713 617 408 405
369 345 52
BsaAI yAC'GTr
Cuts at: 493 1204 6888 493
Size: 711 5684 2702
Fragments arranged by size:
5684 2702 711
BsaBI GATnn'nnATC
Cuts at: 6194 9091 6194
Size: 2897 6200
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2253 2331 4008 4170
Size: 53 83 186 936 720 78 1677 162
Cuts at: 4170 4287 4756 5097 5250 6904 7286 7921 275
Size: 117 469 341 153 1654 382 635 1451
Fragments arranged by size:
1677 1654 1451 936 720 635 469 382
341 186 162 153 117 83 78 53
BsaWI w'CCGG_w
Cuts at: 2519 5739 6260 7530 7928 8202 8349 8760 2519
Size: 3220 521 1270 398 274 147 411 2856
Fragments arranged by size:
3220 2856 1270 521 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8872
Fragments arranged by size:
8872 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 3426 4920 6161 1186
Size: 841 1399 1494 1241 4122
Fragments arranged by size:
4122 1494 1399 1241 841
BseYI C'CCAG_C
Cuts at: 2487 4361 5579 5610 8300 8796 2487
Size: 1874 1218 31 2690 496 2788
Fragments arranged by size:
2788 2690 1874 1218 496 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4227 6232 1163
Size: 573 2491 2005 4028
Fragments arranged by size:
4028 2491 2005 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5512 6547 7308 8336 1540
Size: 275 186 3511 1035 761 1028 2301
Fragments arranged by size:
3511 2301 1035 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2590 2761 2896 2968 3211 3229 3319 3418 3859
Size: 171 135 72 243 18 90 99 441
Cuts at: 3859 4111 4156 4390 4579 4597 4840 5146 5155
Size: 252 45 234 189 18 243 306 9
Cuts at: 5155 5452 7158 7243 8314 2590
Size: 297 1706 85 1071 3373
Fragments arranged by size:
3373 1706 1071 441 306 297 252 243
243 234 189 171 135 99 90 85
72 45 18 18 9
BsmI GAATG_Cn'
Cuts at: 4194 6608 6701 4194
Size: 2414 93 6590
Fragments arranged by size:
6590 2414 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2148 5076 2148
Size: 2928 6169
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2175 2590 2761 2896 2912 2950 2968
Size: 399 385 415 171 135 16 38 18
Cuts at: 2968 3003 3084 3211 3229 3247 3272 3319 3418
Size: 35 81 127 18 18 25 47 99
Cuts at: 3418 3724 3780 3859 4111 4147 4156 4336 4390
Size: 306 56 79 252 36 9 180 54
Cuts at: 4390 4399 4579 4597 4840 5009 5056 5146 5155
Size: 9 180 18 243 169 47 90 9
Cuts at: 5155 5164 5452 5496 5747 6032 6182 6352 6416
Size: 9 288 44 251 285 150 170 64
Cuts at: 6416 7158 7243 8314 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 415 399 385 306 288
285 252 251 243 180 180 171 170
169 150 135 127 99 90 85 81
79 64 56 54 47 47 44 38
36 35 25 18 18 18 18 16
9 9 9 9
BspEI T'CCGG_A
Cuts at: 5739 8760 5739
Size: 3021 6076
BspHI T'CATG_A
Cuts at: 6986 8716 6986
Size: 1730 7367
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2129 5786 5906 6452 844
Size: 1285 3657 120 546 3489
Fragments arranged by size:
3657 3489 1285 546 120
BsrBI CCG'CTC
Cuts at: 1394 1823 6984 1394
Size: 429 5161 3507
Fragments arranged by size:
5161 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 7592 7766 65
Size: 1183 6344 174 1396
Fragments arranged by size:
6344 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2327 3014 3680 4301 5120 5228 7741
Size: 110 517 687 666 621 819 108 2513
Cuts at: 7741 7928 1700
Size: 187 2869
Fragments arranged by size:
2869 2513 819 687 666 621 517 187
110 108
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9097
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9097
BssSI C'ACGA_G
Cuts at: 2562 2967 4389 6250 7157 8169 2562
Size: 405 1422 1861 907 1012 3490
Fragments arranged by size:
3490 1861 1422 1012 907 405
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9097
BstBI TT'CG_AA
Cuts at: 7901 7901
Size: 9097
BstEII G'GTnAC_C
Cuts at: 1184 5105 5330 1184
Size: 3921 225 4951
Fragments arranged by size:
4951 3921 225
BstXI CCAn_nnnn'nTGG
Cuts at: 6146 6146
Size: 9097
BstYI r'GATC_y
Cuts at: 1972 2181 3566 4808 6195 6427 6794 7179 7196
Size: 209 1385 1242 1387 232 367 385 17
Cuts at: 7196 8648 8734 8746 9092 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1387 1385 1242 385 367 346
232 209 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2138 2751 2859 4227 2138
Size: 613 108 1368 7008
Fragments arranged by size:
7008 1368 613 108
BtgI C'CryG_G
Cuts at: 513 1065 2544 2652 2914 3481 3724 4291 4408
Size: 552 1479 108 262 567 243 567 117
Cuts at: 4408 4732 5001 5110 5254 5281 5335 513
Size: 324 269 109 144 27 54 4275
Fragments arranged by size:
4275 1479 567 567 552 324 269 262
243 144 117 109 108 54 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2229 4527 487
Size: 683 56 1003 2298 5057
Fragments arranged by size:
5057 2298 1003 683 56
BtsI GCAGTG_nn'
Cuts at: 6109 6613 7407 7427 6109
Size: 504 794 20 7779
Fragments arranged by size:
7779 794 504 20
ClaI AT'CG_AT
Cuts at: 1554 6324 1554
Size: 4770 4327
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9062
DraI TTT'AAA
Cuts at: 6401 6401
Size: 9097
DraIII CAC_nnn'GTG
Cuts at: 3719 3719
Size: 9097
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5472 5562 8104 809
Size: 507 4156 90 2542 1802
Fragments arranged by size:
4156 2542 1802 507 90
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2068 2392
Size: 54 1425 50 275 18 216 22 324
Cuts at: 2392 4465 4645 5641 6022 6048 6544 7433 9062
Size: 2073 180 996 381 26 496 889 1629
Cuts at: 9062 8
Size: 43
Fragments arranged by size:
2073 1629 1425 996 889 496 381 324
275 216 180 54 50 43 26 22
18
EagI C'GGCC_G
Cuts at: 1537 1812 6544 1537
Size: 275 4732 4090
Fragments arranged by size:
4732 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5807 7027 1353
Size: 4454 1220 3423
Fragments arranged by size:
4454 3423 1220
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7684 8058 8204 387
Size: 1064 138 6095 374 146 1280
Fragments arranged by size:
6095 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2894 3416 3857 4154 4388 2894
Size: 522 441 297 234 7603
Fragments arranged by size:
7603 522 441 297 234
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2628 2658 2964 3114 3414 3792 3810
Size: 393 996 30 306 150 300 378 18
Cuts at: 3810 3873 3942 4017 4044 4166 4170 4404 4602
Size: 63 69 75 27 122 4 234 198
Cuts at: 4602 4989 5336 5421 5581 6158 7160 7757 8544
Size: 387 347 85 160 577 1002 597 787
Cuts at: 8544 8812 1239
Size: 268 1524
Fragments arranged by size:
1524 1002 996 787 597 577 393 387
378 347 306 300 268 234 198 160
150 122 85 75 69 63 30 27
18 4
EcoNI CCTnn'n_nnAGG
Cuts at: 6158 6158
Size: 9097
EcoO109I rG'GnC_Cy
Cuts at: 2339 2375 2384 2549 2612 2648 2657 2666 2675
Size: 36 9 165 63 36 9 9 9
Cuts at: 2675 2909 3000 3080 3081 3098 3251 3268 3269
Size: 234 91 80 1 17 153 17 1
Cuts at: 3269 3377 3521 3863 3908 4070 4223 4727 4961
Size: 108 144 342 45 162 153 504 234
Cuts at: 4961 5006 5276 5357 5393 5429 5493 6163 2339
Size: 45 270 81 36 36 64 670 5273
Fragments arranged by size:
5273 670 504 342 270 234 234 165
162 153 153 144 108 91 81 80
64 63 45 45 36 36 36 36
17 17 9 9 9 9 1 1
EcoRI G'AATT_C
Cuts at: 6362 6406 6784 6362
Size: 44 378 8675
Fragments arranged by size:
8675 378 44
FspI TGC'GCA
Cuts at: 7603 7603
Size: 9097
HaeII r_GCGC'y
Cuts at: 1678 2256 3822 4011 4173 4608 5100 5253 5586
Size: 578 1566 189 162 435 492 153 333
Cuts at: 5586 8244 1678
Size: 2658 2531
Fragments arranged by size:
2658 2531 1566 578 492 435 333 189
162 153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2388 2420 2498 2530 4793
Size: 32 135 32 816 32 78 32 2263
Cuts at: 4793 4825 5182 5214 7738 7770 7812 7844 1373
Size: 32 357 32 2524 32 42 32 2626
Fragments arranged by size:
2626 2524 2263 816 357 135 78 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 6441 6687 669
Size: 988 281 4503 246 3079
Fragments arranged by size:
4503 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9097
HpaI GTT'AAC
Cuts at: 6687 6687
Size: 9097
KasI G'GCGC_C
Cuts at: 2252 4007 4169 5096 5249 2252
Size: 1755 162 927 153 6100
Fragments arranged by size:
6100 1755 927 162 153
KpnI G_GTAC'C
Cuts at: 2146 6422 2146
Size: 4276 4821
MfeI C'AATT_G
Cuts at: 6696 6696
Size: 9097
MluI A'CGCG_T
Cuts at: 8973 8973
Size: 9097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6057 6207 8211 8395 1305
Size: 4752 150 2004 184 2007
Fragments arranged by size:
4752 2007 2004 184 150
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6050 10
Size: 54 1425 343 4218 3057
Fragments arranged by size:
4218 3057 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5637 6326 7055 7414 7573 518
Size: 648 4471 689 729 359 159 2042
Fragments arranged by size:
4471 2042 729 689 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2491 2780 3023 3303 3527 3887 4211 4365
Size: 885 289 243 280 224 360 324 154
Cuts at: 4365 4544 5129 6058 6513 7190 8338 8583 1606
Size: 179 585 929 455 677 1148 245 2120
Fragments arranged by size:
2120 1148 929 885 677 585 455 360
324 289 280 245 243 224 179 154
NarI GG'CG_CC
Cuts at: 2253 4008 4170 5097 5250 2253
Size: 1755 162 927 153 6100
Fragments arranged by size:
6100 1755 927 162 153
NcoI C'CATG_G
Cuts at: 513 1065 2544 2652 5001 513
Size: 552 1479 108 2349 4609
Fragments arranged by size:
4609 2349 1479 552 108
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9097
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9097
NotI GC'GGCC_GC
Cuts at: 6544 6544
Size: 9097
NspI r_CATG'y
Cuts at: 1132 1229 5975 6455 8000 1132
Size: 97 4746 480 1545 2229
Fragments arranged by size:
4746 2229 1545 480 97
PasI CC'CwG_GG
Cuts at: 1348 3103 3265 1348
Size: 1755 162 7180
Fragments arranged by size:
7180 1755 162
PciI A'CATG_T
Cuts at: 7996 7996
Size: 9097
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 9097
PfoI T'CCnGG_A
Cuts at: 2425 2452 3805 3994 4579 5551 2425
Size: 27 1353 189 585 972 5971
Fragments arranged by size:
5971 1353 972 585 189 27
PmeI GTTT'AAAC
Cuts at: 6401 6401
Size: 9097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7166 7198 8679 8711 7166
Size: 32 1481 32 7552
Fragments arranged by size:
7552 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2339 2375 2549 2612 2648 2657 2666 2675 3098
Size: 36 174 63 36 9 9 9 423
Cuts at: 3098 3377 3521 3863 4070 4727 4961 5357 5393
Size: 279 144 342 207 657 234 396 36
Cuts at: 5393 5429 2339
Size: 36 6007
Fragments arranged by size:
6007 657 423 396 342 279 234 207
174 144 63 36 36 36 36 9
9 9
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9097
PsiI TTA'TAA
Cuts at: 6667 6667
Size: 9097
PspOMI G'GGCC_C
Cuts at: 2908 2999 3080 3268 5005 5492 2908
Size: 91 81 188 1737 487 6513
Fragments arranged by size:
6513 1737 487 188 91 81
PspXI vC'TCGA_Gb
Cuts at: 2760 3417 5690 2760
Size: 657 2273 6167
Fragments arranged by size:
6167 2273 657
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 6089 6121 6089
Size: 32 9065
PstI C_TGCA'G
Cuts at: 830 6449 830
Size: 5619 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9097
PvuII CAG'CTG
Cuts at: 2491 2780 4211 6058 2491
Size: 289 1431 1847 5530
Fragments arranged by size:
5530 1847 1431 289
SacI G_AGCT'C
Cuts at: 2896 3418 3859 4156 4390 2896
Size: 522 441 297 234 7603
Fragments arranged by size:
7603 522 441 297 234
SalI G'TCGA_C
Cuts at: 1936 6439 1936
Size: 4503 4594
SanDI GG'GwC_CC
Cuts at: 2339 2375 2549 2657 3521 3863 5357 2339
Size: 36 174 108 864 342 1494 6079
Fragments arranged by size:
6079 1494 864 342 174 108 36
SbfI CC_TGCA'GG
Cuts at: 6449 6449
Size: 9097
ScaI AGT'ACT
Cuts at: 5825 7345 9057 5825
Size: 1520 1712 5865
Fragments arranged by size:
5865 1712 1520
SexAI A'CCwGG_T
Cuts at: 2590 2716 3310 3472 3715 3976 4471 4489 2590
Size: 126 594 162 243 261 495 18 7198
Fragments arranged by size:
7198 594 495 261 243 162 126 18
SfcI C'TryA_G
Cuts at: 826 1045 6226 6445 7580 8261 8452 826
Size: 219 5181 219 1135 681 191 1471
Fragments arranged by size:
5181 1471 1135 681 219 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2364 2391 2746 2916 3132 3276 3555 5175 2364
Size: 27 355 170 216 144 279 1620 6286
Fragments arranged by size:
6286 1620 355 279 216 170 144 27
SfoI GGC'GCC
Cuts at: 2254 4009 4171 5098 5251 2254
Size: 1755 162 927 153 6100
Fragments arranged by size:
6100 1755 927 162 153
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9097
SgrAI Cr'CCGG_yG
Cuts at: 1700 2327 1700
Size: 627 8470
SmaI CCC'GGG
Cuts at: 1872 4852 6424 1872
Size: 2980 1572 4545
Fragments arranged by size:
4545 2980 1572
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2760 3417 4857 5619 5690 5708
Size: 197 926 817 657 1440 762 71 18
Cuts at: 5708 5789 6155 7201 8102 8364 820
Size: 81 366 1046 901 262 1553
Fragments arranged by size:
1553 1440 1046 926 901 817 762 657
366 262 197 81 71 18
SnaBI TAC'GTA
Cuts at: 493 6888 493
Size: 6395 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9097
SphI G_CATG'C
Cuts at: 1132 6455 1132
Size: 5323 3774
SspI AAT'ATT
Cuts at: 5 52 7021 5
Size: 47 6969 2081
Fragments arranged by size:
6969 2081 47
StuI AGG'CCT
Cuts at: 1517 6089 1517
Size: 4572 4525
StyI C'CwwG_G
Cuts at: 513 1065 2343 2369 2544 2599 2608 2652 2832
Size: 552 1278 26 175 55 9 44 180
Cuts at: 2832 3076 3291 3516 3615 4003 5001 5100 5272
Size: 244 215 225 99 388 998 99 172
Cuts at: 5272 5842 5958 6336 6536 513
Size: 570 116 378 200 3074
Fragments arranged by size:
3074 1278 998 570 552 388 378 244
225 215 200 180 175 172 116 99
99 55 44 26 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2166 2323 3815 4643 6011 7124 7141
Size: 1255 586 157 1492 828 1368 1113 17
Cuts at: 7141 7294 7479 7924 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1492 1368 1255 1113 828 586 445
185 157 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5823 7343 9055
Size: 274 80 33 51 1443 3846 1520 1712
Cuts at: 9055 96
Size: 138
Fragments arranged by size:
3846 1712 1520 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2517 2549 9057 9089 2517
Size: 32 6508 32 2525
Fragments arranged by size:
6508 2525 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9097
XbaI T'CTAG_A
Cuts at: 6433 6433
Size: 9097
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2495 2615 3110 3938 4172 5279 5360 6014 6325
Size: 120 495 828 234 1107 81 654 311
Cuts at: 6325 2495
Size: 5267
Fragments arranged by size:
5267 1107 828 654 495 311 234 120
81
XhoI C'TCGA_G
Cuts at: 1943 2760 3417 5690 1943
Size: 817 657 2273 5350
Fragments arranged by size:
5350 2273 817 657
XmaI C'CCGG_G
Cuts at: 1870 4850 6422 1870
Size: 2980 1572 4545
Fragments arranged by size:
4545 2980 1572
XmnI GAAnn'nnTTC
Cuts at: 7226 7226
Size: 9097
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4757 6905 276
Size: 53 83 186 936 3223 2148 2468
Fragments arranged by size:
3223 2468 2148 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI RsrII SacII SapI SrfI SwaI