Restriction map for
pFN21AB4784
(Circular) (Six-base) MAPSORT of: pFN21AB4784.seq Check: 9863 from: 1 to: 5518
pFN21AB4784 5518 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2787 3328 278
Size: 53 83 186 936 1251 541 2468
Fragments arranged by size:
2468 1251 936 541 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2787 2861 1937
Size: 850 74 4594
Fragments arranged by size:
4594 850 74
Acc65I G'GTAC_C
Cuts at: 2839 2839
Size: 5518
AclI AA'CG_TT
Cuts at: 1563 3645 4018 1563
Size: 2082 373 3063
Fragments arranged by size:
3063 2082 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3581 4965 5233 1632
Size: 1949 1384 268 1917
Fragments arranged by size:
1949 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5321
AflIII A'CryG_T
Cuts at: 1203 4417 5394 1203
Size: 3214 977 1327
Fragments arranged by size:
3214 1327 977
AgeI A'CCGG_T
Cuts at: 2538 4349 2538
Size: 1811 3707
AhdI GACnn_n'nnGTC
Cuts at: 4247 4247
Size: 5518
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5518
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5518
AlwNI CAG_nnn'CTG
Cuts at: 2699 2732 3261 4833 2699
Size: 33 529 1572 3384
Fragments arranged by size:
3384 1572 529 33
ApaI G_GGCC'C
Cuts at: 2560 2815 2560
Size: 255 5263
ApaLI G'TGCA_C
Cuts at: 3575 4731 3575
Size: 1156 4362
ApoI r'AATT_y
Cuts at: 1493 2827 3053 3205 5314 1493
Size: 1334 226 152 2109 1697
Fragments arranged by size:
2109 1697 1334 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4072 160
Size: 872 3040 1606
Fragments arranged by size:
3040 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
AvaI C'yCGr_G
Cuts at: 1870 1943 2684 2843 1870
Size: 73 741 159 4545
Fragments arranged by size:
4545 741 159 73
BamHI G'GATC_C
Cuts at: 2848 3215 2848
Size: 367 5151
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2839 4294 618
Size: 325 217 627 1052 1455 1842
Fragments arranged by size:
1842 1455 1052 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2181 2560 2815 2837 1391
Size: 790 379 255 22 4072
Fragments arranged by size:
4072 790 379 255 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2049 928
Size: 971 150 4397
Fragments arranged by size:
4397 971 150
BbvCI CC'TCA_GC
Cuts at: 2612 2612
Size: 5518
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3709 3743 1903
Size: 34 1772 34 3678
Fragments arranged by size:
3678 1772 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3410 4620 3410
Size: 1210 4308
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5518
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2501 4129 136
Size: 107 122 71 631 605 829 1628 1525
Fragments arranged by size:
1628 1525 829 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5513 5513
Size: 5518
BlpI GC'TnA_GC
Cuts at: 2768 2935 2768
Size: 167 5351
Bme1580I G_kGCm'C
Cuts at: 1790 2560 2815 3579 4735 1790
Size: 770 255 764 1156 2573
Fragments arranged by size:
2573 1156 770 764 255
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5518
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3249 4207 442
Size: 416 53 83 399 24 1832 958 1753
Fragments arranged by size:
1832 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 2222 4178 1239
Size: 974 9 1956 2579
Fragments arranged by size:
2579 1956 974 9
Bpu10I CC'TnA_GC
Cuts at: 2612 2612
Size: 5518
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2411 3643 4508 4806 2411
Size: 1232 865 298 3123
Fragments arranged by size:
3123 1232 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2080 4181 882
Size: 617 581 2101 2219
Fragments arranged by size:
2219 2101 617 581
BsaAI yAC'GTr
Cuts at: 493 1204 3309 493
Size: 711 2105 2702
Fragments arranged by size:
2702 2105 711
BsaBI GATnn'nnATC
Cuts at: 2753 5512 2753
Size: 2759 2759
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2046 2784 3325 3707
Size: 53 83 186 936 513 738 541 382
Cuts at: 3707 4342 275
Size: 635 1451
Fragments arranged by size:
1451 936 738 635 541 513 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2538 3951 4349 4623 4770 5181 2538
Size: 1413 398 274 147 411 2875
Fragments arranged by size:
2875 1413 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5293
Fragments arranged by size:
5293 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2777 1186
Size: 1591 3927
BseYI C'CCAG_C
Cuts at: 2145 4721 5217 2145
Size: 2576 496 2446
Fragments arranged by size:
2576 2446 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2723 1163
Size: 573 987 3958
Fragments arranged by size:
3958 987 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2968 3729 4757 1540
Size: 275 186 967 761 1028 2301
Fragments arranged by size:
2301 1028 967 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2058 2181 2427 3579 3664 4735 2058
Size: 123 246 1152 85 1071 2841
Fragments arranged by size:
2841 1152 1071 246 123 85
BsmI GAATG_Cn'
Cuts at: 2695 3029 3122 2695
Size: 334 93 5091
Fragments arranged by size:
5091 334 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2449 2449
Size: 5518
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2181 2427 2560 2815 2837 3579
Size: 399 268 123 246 133 255 22 742
Cuts at: 3579 3664 4735 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 268 255 246 133
123 85 22
BspEI T'CCGG_A
Cuts at: 5181 5181
Size: 5518
BspHI T'CATG_A
Cuts at: 3407 5137 3407
Size: 1730 3788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2276 2538 2873 844
Size: 1432 262 335 3489
Fragments arranged by size:
3489 1432 335 262
BsrBI CCG'CTC
Cuts at: 1394 1823 2772 3405 1394
Size: 429 949 633 3507
Fragments arranged by size:
3507 949 633 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2208 4013 4187 65
Size: 1183 960 1805 174 1396
Fragments arranged by size:
1805 1396 1183 960 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2538 4162 4349 1700
Size: 110 728 1624 187 2869
Fragments arranged by size:
2869 1624 728 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5518
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5518
BssSI C'ACGA_G
Cuts at: 2100 3578 4590 2100
Size: 1478 1012 3028
Fragments arranged by size:
3028 1478 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5518
BstBI TT'CG_AA
Cuts at: 4322 4322
Size: 5518
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5518
BstXI CCAn_nnnn'nTGG
Cuts at: 2153 2153
Size: 5518
BstYI r'GATC_y
Cuts at: 1972 2221 2256 2307 2848 3215 3600 3617 5069
Size: 249 35 51 541 367 385 17 1452
Cuts at: 5069 5155 5167 5513 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 541 385 367 346 249 86
51 35 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4027
Fragments arranged by size:
4027 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4779
Fragments arranged by size:
4779 683 56
BtsI GCAGTG_nn'
Cuts at: 2490 2700 3034 3828 3848 2490
Size: 210 334 794 20 4160
Fragments arranged by size:
4160 794 334 210 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5518
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5483
DraI TTT'AAA
Cuts at: 2822 2822
Size: 5518
DraIII CAC_nnn'GTG
Cuts at: 2416 2416
Size: 5518
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4525 809
Size: 507 3209 1802
Fragments arranged by size:
3209 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2155 2502 2965
Size: 54 1425 50 275 18 325 347 463
Cuts at: 2965 3854 5483 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 463 347 325 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2965 1537
Size: 275 1153 4090
Fragments arranged by size:
4090 1153 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2492 3448 1353
Size: 1139 956 3423
Fragments arranged by size:
3423 1139 956
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4105 4479 4625 387
Size: 1064 138 2516 374 146 1280
Fragments arranged by size:
2516 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2179 2179
Size: 5518
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 2222 3581 4178 4965 5233 1239
Size: 393 581 9 1359 597 787 268 1524
Fragments arranged by size:
1524 1359 787 597 581 393 268 9
EcoO109I rG'GnC_Cy
Cuts at: 2122 2265 2608 2629 2812 2122
Size: 143 343 21 183 4828
Fragments arranged by size:
4828 343 183 143 21
EcoRI G'AATT_C
Cuts at: 2827 3205 2827
Size: 378 5140
FspI TGC'GCA
Cuts at: 4024 4024
Size: 5518
HaeII r_GCGC'y
Cuts at: 1678 4665 1678
Size: 2987 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2097 2129 4159 4191 4233
Size: 32 135 32 525 32 2030 32 42
Cuts at: 4233 4265 1373
Size: 32 2626
Fragments arranged by size:
2626 2030 525 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2862 3108 669
Size: 988 281 924 246 3079
Fragments arranged by size:
3079 988 924 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5518
HpaI GTT'AAC
Cuts at: 3108 3108
Size: 5518
KpnI G_GTAC'C
Cuts at: 2843 2843
Size: 5518
MfeI C'AATT_G
Cuts at: 3117 3117
Size: 5518
MluI A'CGCG_T
Cuts at: 5394 5394
Size: 5518
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2199 4632 4816 1305
Size: 894 2433 184 2007
Fragments arranged by size:
2433 2007 894 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2157 2504 10
Size: 54 1425 343 325 347 3024
Fragments arranged by size:
3024 1425 347 343 325 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3476 3835 3994 518
Size: 648 2310 359 159 2042
Fragments arranged by size:
2310 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2806 2934 3611 4759 5004 1606
Size: 1200 128 677 1148 245 2120
Fragments arranged by size:
2120 1200 1148 677 245 128
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4027
Fragments arranged by size:
4027 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5518
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5518
NotI GC'GGCC_GC
Cuts at: 2965 2965
Size: 5518
NspI r_CATG'y
Cuts at: 1132 1229 2153 2876 4421 1132
Size: 97 924 723 1545 2229
Fragments arranged by size:
2229 1545 924 723 97
PasI CC'CwG_GG
Cuts at: 1348 2261 1348
Size: 913 4605
PciI A'CATG_T
Cuts at: 4417 4417
Size: 5518
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5518
PfoI T'CCnGG_A
Cuts at: 2649 2649
Size: 5518
PmeI GTTT'AAAC
Cuts at: 2822 2822
Size: 5518
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3587 3619 5100 5132 3587
Size: 32 1481 32 3973
Fragments arranged by size:
3973 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2265 2608 2629 2265
Size: 343 21 5154
Fragments arranged by size:
5154 343 21
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5518
PsiI TTA'TAA
Cuts at: 3088 3088
Size: 5518
PspOMI G'GGCC_C
Cuts at: 2556 2811 2556
Size: 255 5263
PstI C_TGCA'G
Cuts at: 830 2870 830
Size: 2040 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5518
PvuII CAG'CTG
Cuts at: 2806 2806
Size: 5518
SacI G_AGCT'C
Cuts at: 2181 2181
Size: 5518
SalI G'TCGA_C
Cuts at: 1936 2860 1936
Size: 924 4594
SbfI CC_TGCA'GG
Cuts at: 2870 2870
Size: 5518
ScaI AGT'ACT
Cuts at: 3766 5478 3766
Size: 1712 3806
SexAI A'CCwGG_T
Cuts at: 2412 2412
Size: 5518
SfcI C'TryA_G
Cuts at: 826 1045 2388 2866 4001 4682 4873 826
Size: 219 1343 478 1135 681 191 1471
Fragments arranged by size:
1471 1343 1135 681 478 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5518
SmaI CCC'GGG
Cuts at: 1872 2845 1872
Size: 973 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2426 3622 4523 4785 820
Size: 197 926 483 1196 901 262 1553
Fragments arranged by size:
1553 1196 926 901 483 262 197
SnaBI TAC'GTA
Cuts at: 493 3309 493
Size: 2816 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5518
SphI G_CATG'C
Cuts at: 1132 2876 1132
Size: 1744 3774
SspI AAT'ATT
Cuts at: 5 52 3442 5
Size: 47 3390 2081
Fragments arranged by size:
3390 2081 47
StuI AGG'CCT
Cuts at: 1517 2070 2434 1517
Size: 553 364 4601
Fragments arranged by size:
4601 553 364
StyI C'CwwG_G
Cuts at: 513 1065 2004 2797 2957 513
Size: 552 939 793 160 3074
Fragments arranged by size:
3074 939 793 552 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2042 3545 3562 3715 3900 4345 325
Size: 1255 462 1503 17 153 185 445 1498
Fragments arranged by size:
1503 1498 1255 462 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3764 5476 96
Size: 274 80 33 51 1443 1787 1712 138
Fragments arranged by size:
1787 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5478 5510 5478
Size: 32 5486
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5518
XbaI T'CTAG_A
Cuts at: 2854 2854
Size: 5518
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2068 2068
Size: 5518
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5518
XmaI C'CCGG_G
Cuts at: 1870 2843 1870
Size: 973 4545
XmnI GAAnn'nnTTC
Cuts at: 2481 3647 2481
Size: 1166 4352
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2785 3326 276
Size: 53 83 186 936 1251 541 2468
Fragments arranged by size:
2468 1251 936 541 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I Bsu36I EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI