Restriction map for
pFN21AB4745
(Circular) (Six-base) MAPSORT of: pFN21AB4745.seq Check: 5673 from: 1 to: 5824
pFN21AB4745 5824 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3634 278
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2844 3167 1937
Size: 907 323 4594
Fragments arranged by size:
4594 907 323
Acc65I G'GTAC_C
Cuts at: 3145 3145
Size: 5824
AclI AA'CG_TT
Cuts at: 1563 3951 4324 1563
Size: 2388 373 3063
Fragments arranged by size:
3063 2388 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2285 3887 5271 5539 1632
Size: 653 1602 1384 268 1917
Fragments arranged by size:
1917 1602 1384 653 268
AfeI AGC'GCT
Cuts at: 2426 2426
Size: 5824
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5627
AflIII A'CryG_T
Cuts at: 1203 4723 5700 1203
Size: 3520 977 1327
Fragments arranged by size:
3520 1327 977
AgeI A'CCGG_T
Cuts at: 4655 4655
Size: 5824
AhdI GACnn_n'nnGTC
Cuts at: 4553 4553
Size: 5824
AleI CACnn'nnGTG
Cuts at: 1166 2702 1166
Size: 1536 4288
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5824
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2168 2200 2168
Size: 32 5792
AlwNI CAG_nnn'CTG
Cuts at: 2101 2614 3567 5139 2101
Size: 513 953 1572 2786
Fragments arranged by size:
2786 1572 953 513
ApaI G_GGCC'C
Cuts at: 2346 2346
Size: 5824
ApaLI G'TGCA_C
Cuts at: 2976 3881 5037 2976
Size: 905 1156 3763
Fragments arranged by size:
3763 1156 905
ApoI r'AATT_y
Cuts at: 1493 3133 3359 3511 5620 1493
Size: 1640 226 152 2109 1697
Fragments arranged by size:
2109 1697 1640 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4378 160
Size: 872 3346 1606
Fragments arranged by size:
3346 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
AvaI C'yCGr_G
Cuts at: 1870 1943 2350 3149 1870
Size: 73 407 799 4545
Fragments arranged by size:
4545 799 407 73
BamHI G'GATC_C
Cuts at: 3154 3521 3154
Size: 367 5457
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3145 4600 618
Size: 325 217 627 1358 1455 1842
Fragments arranged by size:
1842 1455 1358 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2038 2346 2556 3143 1391
Size: 647 308 210 587 4072
Fragments arranged by size:
4072 647 587 308 210
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4853
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4015 4049 1903
Size: 34 2078 34 3678
Fragments arranged by size:
3678 2078 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3716 4926 3716
Size: 1210 4614
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5824
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4435 136
Size: 107 122 71 631 605 2763 1525
Fragments arranged by size:
2763 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5819 5819
Size: 5824
BlpI GC'TnA_GC
Cuts at: 3241 3241
Size: 5824
Bme1580I G_kGCm'C
Cuts at: 1790 2346 2980 3885 5041 1790
Size: 556 634 905 1156 2573
Fragments arranged by size:
2573 1156 905 634 556
BmgBI CAC'GTC
Cuts at: 1318 2869 1318
Size: 1551 4273
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2349 3555 4513
Size: 416 53 83 399 24 932 1206 958
Cuts at: 4513 442
Size: 1753
Fragments arranged by size:
1753 1206 958 932 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5824
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3132 3132
Size: 5824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2484 2660 2804 2942 4484 1239
Size: 1245 176 144 138 1542 2579
Fragments arranged by size:
2579 1542 1245 176 144 138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3949 4814 5112 3949
Size: 865 298 4661
Fragments arranged by size:
4661 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2023 4487 882
Size: 617 524 2464 2219
Fragments arranged by size:
2464 2219 617 524
BsaAI yAC'GTr
Cuts at: 493 1204 2044 3615 493
Size: 711 840 1571 2702
Fragments arranged by size:
2702 1571 840 711
BsaBI GATnn'nnATC
Cuts at: 5818 5818
Size: 5824
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2876 3631 4013 4648
Size: 53 83 186 936 1343 755 382 635
Cuts at: 4648 275
Size: 1451
Fragments arranged by size:
1451 1343 936 755 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2595 4257 4655 4929 5076 5487 2595
Size: 1662 398 274 147 411 2932
Fragments arranged by size:
2932 1662 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2168 2198 1178
Size: 30 165 30 765 30 4804
Fragments arranged by size:
4804 765 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2466 2582 2657 1186
Size: 1280 116 75 4353
Fragments arranged by size:
4353 1280 116 75
BseYI C'CCAG_C
Cuts at: 5027 5523 5027
Size: 496 5328
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2024 2627 2702 2713 1163
Size: 573 288 603 75 11 4274
Fragments arranged by size:
4274 603 573 288 75 11
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3274 4035 5063 1540
Size: 275 186 1273 761 1028 2301
Fragments arranged by size:
2301 1273 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2038 2556 2800 2817 2980 3885 3970 5041 2038
Size: 518 244 17 163 905 85 1071 2821
Fragments arranged by size:
2821 1071 905 518 244 163 85 17
BsmI GAATG_Cn'
Cuts at: 3335 3428 3335
Size: 93 5731
BsmBI CGTCTCn'nnnn_
Cuts at: 3091 3091
Size: 5824
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2038 2346 2556 2800 2817 2980 3143
Size: 399 248 308 210 244 17 163 163
Cuts at: 3143 3885 3970 5041 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 308 248 244 210
163 163 85 17
BspEI T'CCGG_A
Cuts at: 2595 5487 2595
Size: 2892 2932
BspHI T'CATG_A
Cuts at: 3713 5443 3713
Size: 1730 4094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2407 3179 844
Size: 1563 772 3489
Fragments arranged by size:
3489 1563 772
BsrBI CCG'CTC
Cuts at: 1394 1823 2626 3711 1394
Size: 429 803 1085 3507
Fragments arranged by size:
3507 1085 803 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4319 4493 65
Size: 1183 3071 174 1396
Fragments arranged by size:
3071 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2732 2897 3045 4468 4655 1700
Size: 110 922 165 148 1423 187 2869
Fragments arranged by size:
2869 1423 922 187 165 148 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5824
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5824
BssSI C'ACGA_G
Cuts at: 3884 4896 3884
Size: 1012 4812
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2614 794
Size: 1820 4004
BstBI TT'CG_AA
Cuts at: 4628 4628
Size: 5824
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5824
BstXI CCAn_nnnn'nTGG
Cuts at: 2716 3029 2716
Size: 313 5511
BstYI r'GATC_y
Cuts at: 1972 2586 3154 3521 3906 3923 5375 5461 5473
Size: 614 568 367 385 17 1452 86 12
Cuts at: 5473 5819 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 614 568 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2172 2328 2919 2997 513
Size: 552 939 168 156 591 78 3340
Fragments arranged by size:
3340 939 591 552 168 156 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2899 487
Size: 683 56 1673 3412
Fragments arranged by size:
3412 1673 683 56
BtsI GCAGTG_nn'
Cuts at: 2797 3340 4134 4154 2797
Size: 543 794 20 4467
Fragments arranged by size:
4467 794 543 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5824
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5789
DraI TTT'AAA
Cuts at: 3128 3128
Size: 5824
DraIII CAC_nnn'GTG
Cuts at: 2047 2152 2047
Size: 105 5719
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3083 4831 809
Size: 507 1767 1748 1802
Fragments arranged by size:
1802 1767 1748 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2325 2706 2922
Size: 54 1425 50 275 18 495 381 216
Cuts at: 2922 3271 4160 5789 8
Size: 349 889 1629 43
Fragments arranged by size:
1629 1425 889 495 381 349 275 216
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3271 1537
Size: 275 1459 4090
Fragments arranged by size:
4090 1459 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3754 1353
Size: 2401 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2743 4411 4785 4931 387
Size: 1064 138 1154 1668 374 146 1280
Fragments arranged by size:
1668 1280 1154 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2036 2554 2036
Size: 518 5306
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2285 2484 2660 2804 2942 3887 4484
Size: 393 653 199 176 144 138 945 597
Cuts at: 4484 5271 5539 1239
Size: 787 268 1524
Fragments arranged by size:
1524 945 787 653 597 393 268 199
176 144 138
EcoNI CCTnn'n_nnAGG
Cuts at: 2597 2597
Size: 5824
EcoO109I rG'GnC_Cy
Cuts at: 2010 2098 2299 2343 3076 2010
Size: 88 201 44 733 4758
Fragments arranged by size:
4758 733 201 88 44
EcoRI G'AATT_C
Cuts at: 3133 3511 3133
Size: 378 5446
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2155 2248 2155
Size: 93 5731
FspI TGC'GCA
Cuts at: 4330 4330
Size: 5824
HaeII r_GCGC'y
Cuts at: 1678 2428 4971 1678
Size: 750 2543 2531
Fragments arranged by size:
2543 2531 750
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2658 2690 2875 2907 4465
Size: 32 135 32 1086 32 185 32 1558
Cuts at: 4465 4497 4539 4571 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1558 1086 185 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2935 3168 3414 669
Size: 988 281 997 233 246 3079
Fragments arranged by size:
3079 997 988 281 246 233
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5824
HpaI GTT'AAC
Cuts at: 3414 3414
Size: 5824
KpnI G_GTAC'C
Cuts at: 3149 3149
Size: 5824
MfeI C'AATT_G
Cuts at: 3423 3423
Size: 5824
MluI A'CGCG_T
Cuts at: 5700 5700
Size: 5824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4938 5122 1305
Size: 3633 184 2007
Fragments arranged by size:
3633 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2327 2708 2924 10
Size: 54 1425 343 495 381 216 2910
Fragments arranged by size:
2910 1425 495 381 343 216 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2120 2148 2441 2678 2702 2918 3782
Size: 648 954 28 293 237 24 216 864
Cuts at: 3782 4141 4300 518
Size: 359 159 2042
Fragments arranged by size:
2042 954 864 648 359 293 237 216
159 28 24
MspA1I CmG'CkG
Cuts at: 1606 2092 2611 2950 3240 3917 5065 5310 1606
Size: 486 519 339 290 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 519 486 339 290 245
NaeI GCC'GGC
Cuts at: 2899 3047 2899
Size: 148 5676
NcoI C'CATG_G
Cuts at: 513 1065 2004 2997 513
Size: 552 939 993 3340
Fragments arranged by size:
3340 993 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5824
NgoMIV G'CCGG_C
Cuts at: 2897 3045 2897
Size: 148 5676
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5824
NotI GC'GGCC_GC
Cuts at: 3271 3271
Size: 5824
NspI r_CATG'y
Cuts at: 1132 1229 3182 4727 1132
Size: 97 1953 1545 2229
Fragments arranged by size:
2229 1953 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5824
PciI A'CATG_T
Cuts at: 4723 4723
Size: 5824
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2152 1108
Size: 1044 4780
PmeI GTTT'AAAC
Cuts at: 3128 3128
Size: 5824
PmlI CAC'GTG
Cuts at: 2044 2044
Size: 5824
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2168 2200 3893 3925 5406 5438 2168
Size: 32 1693 32 1481 32 2554
Fragments arranged by size:
2554 1693 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2010 3076 2010
Size: 1066 4758
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5824
PsiI TTA'TAA
Cuts at: 3394 3394
Size: 5824
PspOMI G'GGCC_C
Cuts at: 2342 2342
Size: 5824
PstI C_TGCA'G
Cuts at: 830 2097 2289 3176 830
Size: 1267 192 887 3478
Fragments arranged by size:
3478 1267 887 192
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5824
PvuII CAG'CTG
Cuts at: 2092 2611 2092
Size: 519 5305
SacI G_AGCT'C
Cuts at: 2038 2556 2038
Size: 518 5306
SalI G'TCGA_C
Cuts at: 1936 3166 1936
Size: 1230 4594
SanDI GG'GwC_CC
Cuts at: 2010 2010
Size: 5824
SbfI CC_TGCA'GG
Cuts at: 3176 3176
Size: 5824
ScaI AGT'ACT
Cuts at: 4072 5784 4072
Size: 1712 4112
SexAI A'CCwGG_T
Cuts at: 2715 2715
Size: 5824
SfcI C'TryA_G
Cuts at: 826 1045 2093 2285 3172 4307 4988 5179 826
Size: 219 1048 192 887 1135 681 191 1471
Fragments arranged by size:
1471 1135 1048 887 681 219 192 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5824
SmaI CCC'GGG
Cuts at: 1872 2352 3151 1872
Size: 480 799 4545
Fragments arranged by size:
4545 799 480
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3928 4829 5091 820
Size: 197 926 1985 901 262 1553
Fragments arranged by size:
1985 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3615 493
Size: 3122 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5824
SphI G_CATG'C
Cuts at: 1132 3182 1132
Size: 2050 3774
SspI AAT'ATT
Cuts at: 5 52 3748 5
Size: 47 3696 2081
Fragments arranged by size:
3696 2081 47
StuI AGG'CCT
Cuts at: 1517 2396 2963 1517
Size: 879 567 4378
Fragments arranged by size:
4378 879 567
StyI C'CwwG_G
Cuts at: 513 1065 2004 2997 3263 513
Size: 552 939 993 266 3074
Fragments arranged by size:
3074 993 939 552 266
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2045 3851 3868 4021 4206 4651 325
Size: 1255 465 1806 17 153 185 445 1498
Fragments arranged by size:
1806 1498 1255 465 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2670 4070 5782
Size: 274 80 33 51 1443 693 1400 1712
Cuts at: 5782 96
Size: 138
Fragments arranged by size:
1712 1443 1400 693 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2970 3002 5784 5816 2970
Size: 32 2782 32 2978
Fragments arranged by size:
2978 2782 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5824
XbaI T'CTAG_A
Cuts at: 3160 3160
Size: 5824
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2917 2917
Size: 5824
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5824
XmaI C'CCGG_G
Cuts at: 1870 2350 3149 1870
Size: 480 799 4545
Fragments arranged by size:
4545 799 480
XmnI GAAnn'nnTTC
Cuts at: 3953 3953
Size: 5824
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3632 276
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI
NruI NsiI PacI PfoI PspXI PsrI RsrII SacII
SapI SfiI SfoI SrfI SwaI