Restriction map for
pFN21AB4741
(Circular) (Six-base) MAPSORT of: pFN21AB4741.seq Check: 9352 from: 1 to: 8140
pFN21AB4741 8140 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5193 5950 278
Size: 53 83 186 936 3657 757 2468
Fragments arranged by size:
3657 2468 936 757 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5483 1937
Size: 3546 4594
Acc65I G'GTAC_C
Cuts at: 5461 5461
Size: 8140
AclI AA'CG_TT
Cuts at: 1563 3070 6267 6640 1563
Size: 1507 3197 373 3063
Fragments arranged by size:
3197 3063 1507 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4027 4039 4629 4658 5004 5358 5376 6203
Size: 2395 12 590 29 346 354 18 827
Cuts at: 6203 7587 7855 1632
Size: 1384 268 1917
Fragments arranged by size:
2395 1917 1384 827 590 354 346 268
29 18 12
AfeI AGC'GCT
Cuts at: 2155 2155
Size: 8140
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7943
AflIII A'CryG_T
Cuts at: 1203 2201 7039 8016 1203
Size: 998 4838 977 1327
Fragments arranged by size:
4838 1327 998 977
AgeI A'CCGG_T
Cuts at: 6971 6971
Size: 8140
AhdI GACnn_n'nnGTC
Cuts at: 6869 6869
Size: 8140
AleI CACnn'nnGTG
Cuts at: 1166 4080 1166
Size: 2914 5226
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4098 4929 1184
Size: 2914 831 4395
Fragments arranged by size:
4395 2914 831
AlwNI CAG_nnn'CTG
Cuts at: 5883 7455 5883
Size: 1572 6568
ApaLI G'TGCA_C
Cuts at: 4073 6197 7353 4073
Size: 2124 1156 4860
Fragments arranged by size:
4860 2124 1156
ApoI r'AATT_y
Cuts at: 1493 2863 3488 4282 4361 4441 4975 5261 5449
Size: 1370 625 794 79 80 534 286 188
Cuts at: 5449 5675 5827 7936 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1370 794 625 534 286 226
188 152 80 79
AseI AT'TA_AT
Cuts at: 160 1032 2405 4931 6694 160
Size: 872 1373 2526 1763 1606
Fragments arranged by size:
2526 1763 1606 1373 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8140
AvaI C'yCGr_G
Cuts at: 1870 1943 3840 5465 1870
Size: 73 1897 1625 4545
Fragments arranged by size:
4545 1897 1625 73
BamHI G'GATC_C
Cuts at: 4000 4327 5470 5837 4000
Size: 327 1143 367 6303
Fragments arranged by size:
6303 1143 367 327
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3991 5318 5461 6916 618
Size: 325 217 627 2204 1327 143 1455 1842
Fragments arranged by size:
2204 1842 1455 1327 627 325 217 143
BanII G_rGCy'C
Cuts at: 1391 2144 5459 1391
Size: 753 3315 4072
Fragments arranged by size:
4072 3315 753
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3478 928
Size: 971 1579 5590
Fragments arranged by size:
5590 1579 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4179 4213 6331 6365 1903
Size: 34 2242 34 2118 34 3678
Fragments arranged by size:
3678 2242 2118 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6032 7242 6032
Size: 1210 6930
BclI T'GATC_A
Cuts at: 1548 2844 1548
Size: 1296 6844
BfrBI ATG'CAT
Cuts at: 3025 4580 3025
Size: 1555 6585
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6751 136
Size: 107 122 71 631 605 5079 1525
Fragments arranged by size:
5079 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3148 8135 3148
Size: 4987 3153
BlpI GC'TnA_GC
Cuts at: 5557 5557
Size: 8140
Bme1580I G_kGCm'C
Cuts at: 1790 3839 4077 5323 6201 7357 1790
Size: 2049 238 1246 878 1156 2573
Fragments arranged by size:
2573 2049 1246 1156 878 238
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8140
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4216 4329 4756
Size: 416 53 83 399 24 2799 113 427
Cuts at: 4756 5871 6829 442
Size: 1115 958 1753
Fragments arranged by size:
2799 1753 1115 958 427 416 399 113
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8140
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5028 5028
Size: 8140
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3228 3751 4411 4915 6800 1239
Size: 1989 523 660 504 1885 2579
Fragments arranged by size:
2579 1989 1885 660 523 504
Bpu10I CC'TnA_GC
Cuts at: 4184 4626 4184
Size: 442 7698
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6265 7130 7428 6265
Size: 865 298 6977
Fragments arranged by size:
6977 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4637 4716 6803 882
Size: 617 3138 79 2087 2219
Fragments arranged by size:
3138 2219 2087 617 79
BsaAI yAC'GTr
Cuts at: 493 1204 5931 493
Size: 711 4727 2702
Fragments arranged by size:
4727 2702 711
BsaBI GATnn'nnATC
Cuts at: 8134 8134
Size: 8140
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5190 5947 6329 6964
Size: 53 83 186 936 3657 757 382 635
Cuts at: 6964 275
Size: 1451
Fragments arranged by size:
3657 1451 936 757 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6573 6971 7245 7392 7803 6573
Size: 398 274 147 411 6910
Fragments arranged by size:
6910 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3843 3873 1178
Size: 30 165 30 2440 30 5445
Fragments arranged by size:
5445 2440 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2961 4004 1186
Size: 1775 1043 5322
Fragments arranged by size:
5322 1775 1043
BseYI C'CCAG_C
Cuts at: 7343 7839 7343
Size: 496 7644
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2718 3678 3828 1163
Size: 573 982 960 150 5475
Fragments arranged by size:
5475 982 960 573 150
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5590 6351 7379 1540
Size: 275 186 3589 761 1028 2301
Fragments arranged by size:
3589 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2971 4077 4741 6201 6286 7357 2971
Size: 1106 664 1460 85 1071 3754
Fragments arranged by size:
3754 1460 1106 1071 664 85
BsmI GAATG_Cn'
Cuts at: 4042 5651 5744 4042
Size: 1609 93 6438
Fragments arranged by size:
6438 1609 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4350 4350
Size: 8140
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2144 2971 3839 4077 4741 5323 5459
Size: 399 354 827 868 238 664 582 136
Cuts at: 5459 6201 6286 7357 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 868 827 742 664 582 399
354 238 136 85
BspEI T'CCGG_A
Cuts at: 7803 7803
Size: 8140
BspHI T'CATG_A
Cuts at: 6029 7759 6029
Size: 1730 6410
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5495 844
Size: 4651 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 6027 1394
Size: 429 4204 3507
Fragments arranged by size:
4204 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2910 2949 3687 5193 6635 6809 65
Size: 1183 1662 39 738 1506 1442 174 1396
Fragments arranged by size:
1662 1506 1442 1396 1183 738 174 39
BsrFI r'CCGG_y
Cuts at: 1700 1810 6784 6971 1700
Size: 110 4974 187 2869
Fragments arranged by size:
4974 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2453 96
Size: 2357 5783
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8140
BssSI C'ACGA_G
Cuts at: 5371 6200 7212 5371
Size: 829 1012 6299
Fragments arranged by size:
6299 1012 829
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8140
BstBI TT'CG_AA
Cuts at: 6944 6944
Size: 8140
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8140
BstYI r'GATC_y
Cuts at: 1972 3148 3344 4000 4327 5036 5470 5837 6222
Size: 1176 196 656 327 709 434 367 385
Cuts at: 6222 6239 7691 7777 7789 8135 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1176 709 656 434 385 367
346 327 196 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3996 3996
Size: 8140
BtgI C'CryG_G
Cuts at: 513 1065 3503 3985 513
Size: 552 2438 482 4668
Fragments arranged by size:
4668 2438 552 482
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7401
Fragments arranged by size:
7401 683 56
BtsI GCAGTG_nn'
Cuts at: 4075 4087 5023 5656 6450 6470 4075
Size: 12 936 633 794 20 5745
Fragments arranged by size:
5745 936 794 633 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8140
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3745 3780 529
Size: 35 3181 35 4889
Fragments arranged by size:
4889 3181 35 35
DraI TTT'AAA
Cuts at: 2490 2868 5427 5444 2490
Size: 378 2559 17 5186
Fragments arranged by size:
5186 2559 378 17
DraIII CAC_nnn'GTG
Cuts at: 3991 4337 5318 3991
Size: 346 981 6813
Fragments arranged by size:
6813 981 346
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7147 809
Size: 507 5831 1802
Fragments arranged by size:
5831 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3818 3977 5587
Size: 54 1425 50 275 18 1988 159 1610
Cuts at: 5587 6476 8105 8
Size: 889 1629 43
Fragments arranged by size:
1988 1629 1610 1425 889 275 159 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5587 1537
Size: 275 3775 4090
Fragments arranged by size:
4090 3775 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3825 3940 5091 6070 1353
Size: 2472 115 1151 979 3423
Fragments arranged by size:
3423 2472 1151 979 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6727 7101 7247 387
Size: 1064 138 5138 374 146 1280
Fragments arranged by size:
5138 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3228 3751 4027 4039 4411 4629 4658
Size: 393 1596 523 276 12 372 218 29
Cuts at: 4658 4915 5004 5358 5376 6203 6800 7587 7855
Size: 257 89 354 18 827 597 787 268
Cuts at: 7855 1239
Size: 1524
Fragments arranged by size:
1596 1524 827 787 597 523 393 372
354 276 268 257 218 89 29 18
12
EcoO109I rG'GnC_Cy
Cuts at: 3701 3701
Size: 8140
EcoRI G'AATT_C
Cuts at: 4361 4441 4975 5449 5827 4361
Size: 80 534 474 378 6674
Fragments arranged by size:
6674 534 474 378 80
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3491 5439 3491
Size: 1948 6192
FspI TGC'GCA
Cuts at: 6646 6646
Size: 8140
HaeII r_GCGC'y
Cuts at: 1678 2157 7287 1678
Size: 479 5130 2531
Fragments arranged by size:
5130 2531 479
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3617 3649 4517 4549 4609
Size: 32 135 32 2045 32 868 32 60
Cuts at: 4609 4641 6781 6813 6855 6887 1373
Size: 32 2140 32 42 32 2626
Fragments arranged by size:
2626 2140 2045 868 135 60 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3574 4786 5484 5730 669
Size: 988 281 1636 1212 698 246 3079
Fragments arranged by size:
3079 1636 1212 988 698 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8140
HpaI GTT'AAC
Cuts at: 5730 5730
Size: 8140
KpnI G_GTAC'C
Cuts at: 5465 5465
Size: 8140
MfeI C'AATT_G
Cuts at: 5739 5739
Size: 8140
MluI A'CGCG_T
Cuts at: 8016 8016
Size: 8140
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3417 7254 7438 1305
Size: 2112 3837 184 2007
Fragments arranged by size:
3837 2112 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3979 10
Size: 54 1425 343 2147 4171
Fragments arranged by size:
4171 2147 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2228 4070 4080 6098 6457 6616
Size: 648 843 219 1842 10 2018 359 159
Cuts at: 6616 518
Size: 2042
Fragments arranged by size:
2042 2018 1842 843 648 359 219 159
10
MspA1I CmG'CkG
Cuts at: 1606 3505 3975 5556 6233 7381 7626 1606
Size: 1899 470 1581 677 1148 245 2120
Fragments arranged by size:
2120 1899 1581 1148 677 470 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7588
NdeI CA'TA_TG
Cuts at: 387 4459 4768 387
Size: 4072 309 3759
Fragments arranged by size:
4072 3759 309
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8140
NotI GC'GGCC_GC
Cuts at: 5587 5587
Size: 8140
NsiI A_TGCA'T
Cuts at: 3027 4582 3027
Size: 1555 6585
NspI r_CATG'y
Cuts at: 1132 1229 2205 2903 3029 5498 7043 1132
Size: 97 976 698 126 2469 1545 2229
Fragments arranged by size:
2469 2229 1545 976 698 126 97
PasI CC'CwG_GG
Cuts at: 1348 3045 3705 1348
Size: 1697 660 5783
Fragments arranged by size:
5783 1697 660
PciI A'CATG_T
Cuts at: 2201 7039 2201
Size: 4838 3302
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8140
PfoI T'CCnGG_A
Cuts at: 2695 3205 2695
Size: 510 7630
PmeI GTTT'AAAC
Cuts at: 5444 5444
Size: 8140
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6209 6241 7722 7754 6209
Size: 32 1481 32 6595
Fragments arranged by size:
6595 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3701 3701
Size: 8140
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8140
PsiI TTA'TAA
Cuts at: 3243 3447 3594 4589 5710 3243
Size: 204 147 995 1121 5673
Fragments arranged by size:
5673 1121 995 204 147
PstI C_TGCA'G
Cuts at: 830 4082 4918 5030 5492 830
Size: 3252 836 112 462 3478
Fragments arranged by size:
3478 3252 836 462 112
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8140
PvuII CAG'CTG
Cuts at: 3975 3975
Size: 8140
SacII CC_GC'GG
Cuts at: 3506 3506
Size: 8140
SalI G'TCGA_C
Cuts at: 1936 5482 1936
Size: 3546 4594
SbfI CC_TGCA'GG
Cuts at: 5492 5492
Size: 8140
ScaI AGT'ACT
Cuts at: 4745 6388 8100 4745
Size: 1643 1712 4785
Fragments arranged by size:
4785 1712 1643
SfcI C'TryA_G
Cuts at: 826 1045 2539 4078 4914 5026 5488 6623 7304
Size: 219 1494 1539 836 112 462 1135 681
Cuts at: 7304 7495 826
Size: 191 1471
Fragments arranged by size:
1539 1494 1471 1135 836 681 462 219
191 112
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8140
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8140
SmaI CCC'GGG
Cuts at: 1872 5467 1872
Size: 3595 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6244 7145 7407 820
Size: 197 926 4301 901 262 1553
Fragments arranged by size:
4301 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5931 493
Size: 5438 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8140
SphI G_CATG'C
Cuts at: 1132 5498 1132
Size: 4366 3774
SspI AAT'ATT
Cuts at: 5 52 2409 2503 2533 3158 5055 6064 5
Size: 47 2357 94 30 625 1897 1009 2081
Fragments arranged by size:
2357 2081 1897 1009 625 94 47 30
StuI AGG'CCT
Cuts at: 1517 3040 3182 4137 1517
Size: 1523 142 955 5520
Fragments arranged by size:
5520 1523 955 142
StyI C'CwwG_G
Cuts at: 513 1065 4923 5579 513
Size: 552 3858 656 3074
Fragments arranged by size:
3858 3074 656 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4348 6167 6184 6337 6522 6967 325
Size: 1255 2768 1819 17 153 185 445 1498
Fragments arranged by size:
2768 1819 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2384 2453 4224
Size: 274 80 33 51 1443 407 69 1771
Cuts at: 4224 4743 5006 6386 8098 96
Size: 519 263 1380 1712 138
Fragments arranged by size:
1771 1712 1443 1380 519 407 274 263
138 80 69 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3790 3822 3936 3968 8100 8132 3790
Size: 32 114 32 4132 32 3798
Fragments arranged by size:
4132 3798 114 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8140
XbaI T'CTAG_A
Cuts at: 5476 5476
Size: 8140
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2982 2982
Size: 8140
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8140
XmaI C'CCGG_G
Cuts at: 1870 5465 1870
Size: 3595 4545
XmnI GAAnn'nnTTC
Cuts at: 2436 4040 6269 2436
Size: 1604 2229 4307
Fragments arranged by size:
4307 2229 1604
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5191 5948 276
Size: 53 83 186 936 3657 757 2468
Fragments arranged by size:
3657 2468 936 757 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BsiWI BstXI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI
PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI
SfoI SrfI SwaI