Restriction map for
pFN21AB4737
(Circular) (Six-base) MAPSORT of: pFN21AB4737.seq Check: 7691 from: 1 to: 8803
pFN21AB4737 8803 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2381 4874 2381
Size: 2493 6310
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6613 278
Size: 53 83 186 936 5077 2468
Fragments arranged by size:
5077 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4654 5583 6146 1937
Size: 2717 929 563 4594
Fragments arranged by size:
4594 2717 929 563
Acc65I G'GTAC_C
Cuts at: 6124 6124
Size: 8803
AclI AA'CG_TT
Cuts at: 1563 6930 7303 1563
Size: 5367 373 3063
Fragments arranged by size:
5367 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5441 5481 6866 8250 8518 1632
Size: 3809 40 1385 1384 268 1917
Fragments arranged by size:
3809 1917 1385 1384 268 40
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8606
AflIII A'CryG_T
Cuts at: 1203 2674 5677 7702 8679 1203
Size: 1471 3003 2025 977 1327
Fragments arranged by size:
3003 2025 1471 1327 977
AgeI A'CCGG_T
Cuts at: 7634 7634
Size: 8803
AhdI GACnn_n'nnGTC
Cuts at: 7532 7532
Size: 8803
AleI CACnn'nnGTG
Cuts at: 1166 5118 1166
Size: 3952 4851
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8803
AlwNI CAG_nnn'CTG
Cuts at: 3575 3673 3854 6546 8118 3575
Size: 98 181 2692 1572 4260
Fragments arranged by size:
4260 2692 1572 181 98
ApaI G_GGCC'C
Cuts at: 2105 3108 3260 4720 5052 5168 5623 2105
Size: 1003 152 1460 332 116 455 5285
Fragments arranged by size:
5285 1460 1003 455 332 152 116
ApaLI G'TGCA_C
Cuts at: 4887 6860 8016 4887
Size: 1973 1156 5674
Fragments arranged by size:
5674 1973 1156
ApoI r'AATT_y
Cuts at: 1493 6068 6112 6338 6490 8599 1493
Size: 4575 44 226 152 2109 1697
Fragments arranged by size:
4575 2109 1697 226 152 44
AseI AT'TA_AT
Cuts at: 160 1032 7357 160
Size: 872 6325 1606
Fragments arranged by size:
6325 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8803
AvaI C'yCGr_G
Cuts at: 1870 1943 3558 4125 4636 6128 1870
Size: 73 1615 567 511 1492 4545
Fragments arranged by size:
4545 1615 1492 567 511 73
AvrII C'CTAG_G
Cuts at: 5159 5159
Size: 8803
BamHI G'GATC_C
Cuts at: 6133 6500 6133
Size: 367 8436
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2609 2681 2699 2780 2924
Size: 325 217 627 822 72 18 81 144
Cuts at: 2924 3023 4049 6124 7579 618
Size: 99 1026 2075 1455 1842
Fragments arranged by size:
2075 1842 1455 1026 822 627 325 217
144 99 81 72 18
BanII G_rGCy'C
Cuts at: 1391 2105 2326 2650 2965 3093 3108 3260 4720
Size: 714 221 324 315 128 15 152 1460
Cuts at: 4720 5037 5052 5168 5623 5663 5888 6122 1391
Size: 317 15 116 455 40 225 234 4072
Fragments arranged by size:
4072 1460 714 455 324 317 315 234
225 221 152 128 116 40 15 15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2855 3215 3350 4592 5051 928
Size: 971 956 360 135 1242 459 4680
Fragments arranged by size:
4680 1242 971 956 459 360 135
BbvCI CC'TCA_GC
Cuts at: 5910 5910
Size: 8803
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6994 7028 1903
Size: 34 50 34 4973 34 3678
Fragments arranged by size:
4973 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2014 5624 6695 7905 2014
Size: 3610 1071 1210 2912
Fragments arranged by size:
3610 2912 1210 1071
BclI T'GATC_A
Cuts at: 1548 5122 1548
Size: 3574 5229
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2154 2418 2769
Size: 107 122 71 631 605 482 264 351
Cuts at: 2769 2877 2949 3417 3444 5010 5271 7414 136
Size: 108 72 468 27 1566 261 2143 1525
Fragments arranged by size:
2143 1566 1525 631 605 482 468 351
264 261 122 108 107 72 71 27
BglII A'GATC_T
Cuts at: 8798 8798
Size: 8803
BlpI GC'TnA_GC
Cuts at: 2009 5374 6220 2009
Size: 3365 846 4592
Fragments arranged by size:
4592 3365 846
Bme1580I G_kGCm'C
Cuts at: 1790 2105 2614 2686 2704 2785 3108 3260 3899
Size: 315 509 72 18 81 323 152 639
Cuts at: 3899 4054 4720 4891 5052 5168 5623 6864 8020
Size: 155 666 171 161 116 455 1241 1156
Cuts at: 8020 1790
Size: 2573
Fragments arranged by size:
2573 1241 1156 666 639 509 455 323
315 171 161 155 152 116 81 72
18
BmgBI CAC'GTC
Cuts at: 1318 2675 5680 1318
Size: 1357 3005 4441
Fragments arranged by size:
4441 3005 1357
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3120 6083 6534
Size: 416 53 83 399 24 1703 2963 451
Cuts at: 6534 7492 442
Size: 958 1753
Fragments arranged by size:
2963 1753 1703 958 451 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8803
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3112 3112
Size: 8803
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2340 2616 2751 2925 3165 3249 3312 3474
Size: 1101 276 135 174 240 84 63 162
Cuts at: 3474 3552 3642 3978 4086 4518 4569 4572 4770
Size: 78 90 336 108 432 51 3 198
Cuts at: 4770 5289 7463 1239
Size: 519 2174 2579
Fragments arranged by size:
2579 2174 1101 519 432 336 276 240
198 174 162 135 108 90 84 78
63 51 3
Bpu10I CC'TnA_GC
Cuts at: 3594 5910 3594
Size: 2316 6487
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2319 5521 6928 7793 8091 2319
Size: 3202 1407 865 298 3031
Fragments arranged by size:
3202 3031 1407 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2225 3344 3731 3749 3992 4631 5438
Size: 617 726 1119 387 18 243 639 807
Cuts at: 5438 7466 882
Size: 2028 2219
Fragments arranged by size:
2219 2028 1119 807 726 639 617 387
243 18
BsaAI yAC'GTr
Cuts at: 493 1204 2126 6594 493
Size: 711 922 4468 2702
Fragments arranged by size:
4468 2702 922 711
BsaBI GATnn'nnATC
Cuts at: 8797 8797
Size: 8803
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3249 6610 6992 7627
Size: 53 83 186 936 1716 3361 382 635
Cuts at: 7627 275
Size: 1451
Fragments arranged by size:
3361 1716 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 7236 7634 7908 8055 8466 7236
Size: 398 274 147 411 7573
Fragments arranged by size:
7573 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2170 2200 5515 5545 1178
Size: 30 165 30 767 30 3315 30 4436
Fragments arranged by size:
4436 3315 767 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2487 3477 4242 5861 1186
Size: 1301 990 765 1619 4128
Fragments arranged by size:
4128 1619 1301 990 765
BseYI C'CCAG_C
Cuts at: 2104 2692 2957 3101 4091 4294 4442 5479 5638
Size: 588 265 144 990 203 148 1037 159
Cuts at: 5638 8006 8502 2104
Size: 2368 496 2405
Fragments arranged by size:
2405 2368 1037 990 588 496 265 203
159 148 144
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2334 4530 1163
Size: 573 598 2196 5436
Fragments arranged by size:
5436 2196 598 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6253 7014 8042 1540
Size: 275 186 4252 761 1028 2301
Fragments arranged by size:
4252 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2398 2497 2587 3559 3568 3595 4027 4036 4612
Size: 99 90 972 9 27 432 9 576
Cuts at: 4612 4765 4891 5134 5663 6864 6949 8020 2398
Size: 153 126 243 529 1201 85 1071 3181
Fragments arranged by size:
3181 1201 1071 972 576 529 432 243
153 126 99 90 85 27 9 9
BsmI GAATG_Cn'
Cuts at: 5013 5238 6314 6407 5013
Size: 225 1076 93 7409
Fragments arranged by size:
7409 1076 225 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2105 2326 2398 2497 2587 2614 2650
Size: 399 315 221 72 99 90 27 36
Cuts at: 2650 2686 2704 2785 2965 3093 3108 3260 3559
Size: 36 18 81 180 128 15 152 299
Cuts at: 3559 3568 3595 3899 4027 4036 4054 4612 4720
Size: 9 27 304 128 9 18 558 108
Cuts at: 4720 4765 4891 5037 5052 5134 5168 5623 5663
Size: 45 126 146 15 82 34 455 40
Cuts at: 5663 5888 6122 6864 6949 8020 1391
Size: 225 234 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 558 455 399 315 304
299 234 225 221 180 152 146 128
128 126 108 99 90 85 82 81
72 45 40 36 36 34 27 27
18 18 15 15 9 9
BspEI T'CCGG_A
Cuts at: 8466 8466
Size: 8803
BspHI T'CATG_A
Cuts at: 6692 8422 6692
Size: 1730 7073
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2359 2381 4874 5239 5913 6158 844
Size: 1515 22 2493 365 674 245 3489
Fragments arranged by size:
3489 2493 1515 674 365 245 22
BsrBI CCG'CTC
Cuts at: 1394 1823 3075 6690 1394
Size: 429 1252 3615 3507
Fragments arranged by size:
3615 3507 1252 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4698 5829 7298 7472 65
Size: 1183 3450 1131 1469 174 1396
Fragments arranged by size:
3450 1469 1396 1183 1131 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2840 3011 3173 3803 4703 7447 7634
Size: 110 1030 171 162 630 900 2744 187
Cuts at: 7634 1700
Size: 2869
Fragments arranged by size:
2869 2744 1030 900 630 187 171 162
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8803
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8803
BssSI C'ACGA_G
Cuts at: 2397 2496 3216 4026 4467 6863 7875 2397
Size: 99 720 810 441 2396 1012 3325
Fragments arranged by size:
3325 2396 1012 810 720 441 99
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5095 794
Size: 4301 4502
BstBI TT'CG_AA
Cuts at: 7607 7607
Size: 8803
BstEII G'GTnAC_C
Cuts at: 1184 2438 4004 4076 4472 4850 5111 5255 1184
Size: 1254 1566 72 396 378 261 144 4732
Fragments arranged by size:
4732 1566 1254 396 378 261 144 72
BstXI CCAn_nnnn'nTGG
Cuts at: 5752 5752
Size: 8803
BstYI r'GATC_y
Cuts at: 1972 3167 6133 6500 6885 6902 8354 8440 8452
Size: 1195 2966 367 385 17 1452 86 12
Cuts at: 8452 8798 1972
Size: 346 1977
Fragments arranged by size:
2966 1977 1452 1195 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 5584 5584
Size: 8803
Bsu36I CC'TnA_GG
Cuts at: 3685 4242 4593 5250 3685
Size: 557 351 657 7238
Fragments arranged by size:
7238 657 557 351
BtgI C'CryG_G
Cuts at: 513 1065 2650 2704 2730 2848 2983 3730 4720
Size: 552 1585 54 26 118 135 747 990
Cuts at: 4720 4908 4936 5116 5142 513
Size: 188 28 180 26 4174
Fragments arranged by size:
4174 1585 990 747 552 188 180 135
118 54 28 26 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4833 487
Size: 683 56 3607 4457
Fragments arranged by size:
4457 3607 683 56
BtsI GCAGTG_nn'
Cuts at: 2716 3897 5533 5815 6319 7113 7133 2716
Size: 1181 1636 282 504 794 20 4386
Fragments arranged by size:
4386 1636 1181 794 504 282 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8803
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8768
DraI TTT'AAA
Cuts at: 6107 6107
Size: 8803
DraIII CAC_nnn'GTG
Cuts at: 2312 2492 3617 2312
Size: 180 1125 7498
Fragments arranged by size:
7498 1125 180
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2553 3354 4389 7810 809
Size: 507 1237 801 1035 3421 1802
Fragments arranged by size:
3421 1802 1237 1035 801 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4489 5784 6250
Size: 54 1425 50 275 18 2659 1295 466
Cuts at: 6250 7139 8768 8
Size: 889 1629 43
Fragments arranged by size:
2659 1629 1425 1295 889 466 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6250 1537
Size: 275 4438 4090
Fragments arranged by size:
4438 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 6733 1353
Size: 5380 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7390 7764 7910 387
Size: 1064 138 5801 374 146 1280
Fragments arranged by size:
5801 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5661 5661
Size: 8803
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2340 2616 2751 2925 3165 3249 3312
Size: 393 708 276 135 174 240 84 63
Cuts at: 3312 3474 3552 3642 3978 4086 4518 4569 4572
Size: 162 78 90 336 108 432 51 3
Cuts at: 4572 4770 5289 5441 5481 6866 7463 8250 8518
Size: 198 519 152 40 1385 597 787 268
Cuts at: 8518 1239
Size: 1524
Fragments arranged by size:
1524 1385 787 708 597 519 432 393
336 276 268 240 198 174 162 152
135 108 90 84 78 63 51 40
3
EcoNI CCTnn'n_nnAGG
Cuts at: 5553 5864 5553
Size: 311 8492
EcoO109I rG'GnC_Cy
Cuts at: 2101 2147 2183 2294 2330 2348 2726 2735 3105
Size: 46 36 111 36 18 378 9 370
Cuts at: 3105 3113 3149 3185 3257 3302 3347 3401 3500
Size: 8 36 36 72 45 45 54 99
Cuts at: 3500 3572 3599 3644 3689 3833 3851 4238 4274
Size: 72 27 45 45 144 18 387 36
Cuts at: 4274 4346 4400 4544 4589 4716 4913 4994 5048
Size: 72 54 144 45 127 197 81 54
Cuts at: 5048 5147 5164 5165 5246 5264 2101
Size: 99 17 1 81 18 5640
Fragments arranged by size:
5640 387 378 370 197 144 144 127
111 99 99 81 81 72 72 72
54 54 54 46 45 45 45 45
45 36 36 36 36 36 27 18
18 18 17 9 8 1
EcoRI G'AATT_C
Cuts at: 6068 6112 6490 6068
Size: 44 378 8381
Fragments arranged by size:
8381 378 44
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2879 4472 2879
Size: 1593 7210
FspI TGC'GCA
Cuts at: 7309 7309
Size: 8803
HaeII r_GCGC'y
Cuts at: 1678 7950 1678
Size: 6272 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3190 3222 3234 3266 5682
Size: 32 135 32 1618 32 12 32 2416
Cuts at: 5682 5714 5939 5971 7444 7476 7518 7550 1373
Size: 32 225 32 1473 32 42 32 2626
Fragments arranged by size:
2626 2416 1618 1473 225 135 42 32
32 32 32 32 32 32 32 12
HincII GTy'rAC
Cuts at: 669 1657 1938 4205 4756 4826 6147 6393 669
Size: 988 281 2267 551 70 1321 246 3079
Fragments arranged by size:
3079 2267 1321 988 551 281 246 70
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8803
HpaI GTT'AAC
Cuts at: 6393 6393
Size: 8803
KpnI G_GTAC'C
Cuts at: 6128 6128
Size: 8803
MfeI C'AATT_G
Cuts at: 3441 6402 3441
Size: 2961 5842
MluI A'CGCG_T
Cuts at: 8679 8679
Size: 8803
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2242 2461 2866 4261 5206 7917 8101 1305
Size: 937 219 405 1395 945 2711 184 2007
Fragments arranged by size:
2711 2007 1395 945 937 405 219 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5786 10
Size: 54 1425 343 3954 3027
Fragments arranged by size:
3954 3027 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5118 6761 7120 7279 518
Size: 648 3952 1643 359 159 2042
Fragments arranged by size:
3952 2042 1643 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2318 2408 2696 2985 3056 3389 3452 3650
Size: 712 90 288 289 71 333 63 198
Cuts at: 3650 3695 4298 6219 6896 8044 8289 1606
Size: 45 603 1921 677 1148 245 2120
Fragments arranged by size:
2120 1921 1148 712 677 603 333 289
288 245 198 90 71 63 45
NaeI GCC'GGC
Cuts at: 3175 4705 3175
Size: 1530 7273
NcoI C'CATG_G
Cuts at: 513 1065 3730 5116 513
Size: 552 2665 1386 4200
Fragments arranged by size:
4200 2665 1386 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8803
NgoMIV G'CCGG_C
Cuts at: 3173 4703 3173
Size: 1530 7273
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8803
NotI GC'GGCC_GC
Cuts at: 6250 6250
Size: 8803
NruI TCG'CGA
Cuts at: 4818 4818
Size: 8803
NspI r_CATG'y
Cuts at: 1132 1229 2070 5493 6161 7706 1132
Size: 97 841 3423 668 1545 2229
Fragments arranged by size:
3423 2229 1545 841 668 97
PasI CC'CwG_GG
Cuts at: 1348 3326 3848 4793 1348
Size: 1978 522 945 5358
Fragments arranged by size:
5358 1978 945 522
PciI A'CATG_T
Cuts at: 7702 7702
Size: 8803
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5649 1108
Size: 4541 4262
PfoI T'CCnGG_A
Cuts at: 2515 2902 3181 3226 3604 4747 5296 2515
Size: 387 279 45 378 1143 549 6022
Fragments arranged by size:
6022 1143 549 387 378 279 45
PmeI GTTT'AAAC
Cuts at: 6107 6107
Size: 8803
PmlI CAC'GTG
Cuts at: 2126 2126
Size: 8803
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3972 4004 6872 6904 8385 8417 3972
Size: 32 2868 32 1481 32 4358
Fragments arranged by size:
4358 2868 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2294 2348 2726 2735 3113 3185 3302 3347 3401
Size: 54 378 9 378 72 117 45 54
Cuts at: 3401 3572 3599 3644 3689 3833 4400 4544 4589
Size: 171 27 45 45 144 567 144 45
Cuts at: 4589 4913 4994 5147 2294
Size: 324 81 153 5950
Fragments arranged by size:
5950 567 378 378 324 171 153 144
144 117 81 72 54 54 45 45
45 45 27 9
PshAI GACnn'nnGTC
Cuts at: 1613 4479 1613
Size: 2866 5937
PsiI TTA'TAA
Cuts at: 6373 6373
Size: 8803
PspOMI G'GGCC_C
Cuts at: 2101 3104 3256 4716 5048 5164 5619 2101
Size: 1003 152 1460 332 116 455 5285
Fragments arranged by size:
5285 1460 1003 455 332 152 116
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3196 3228 4638 4670 5795 5827 3196
Size: 32 1410 32 1125 32 6172
Fragments arranged by size:
6172 1410 1125 32 32 32
PstI C_TGCA'G
Cuts at: 830 2392 4885 6155 830
Size: 1562 2493 1270 3478
Fragments arranged by size:
3478 2493 1562 1270
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8803
PvuII CAG'CTG
Cuts at: 2318 2408 2696 3056 4298 2318
Size: 90 288 360 1242 6823
Fragments arranged by size:
6823 1242 360 288 90
SacI G_AGCT'C
Cuts at: 5663 5663
Size: 8803
SacII CC_GC'GG
Cuts at: 2986 2986
Size: 8803
SalI G'TCGA_C
Cuts at: 1936 6145 1936
Size: 4209 4594
SanDI GG'GwC_CC
Cuts at: 2726 3347 3644 4544 2726
Size: 621 297 900 6985
Fragments arranged by size:
6985 900 621 297
SbfI CC_TGCA'GG
Cuts at: 2392 6155 2392
Size: 3763 5040
ScaI AGT'ACT
Cuts at: 7051 8763 7051
Size: 1712 7091
SexAI A'CCwGG_T
Cuts at: 2197 2308 2371 2461 2605 3874 3982 4207 4657
Size: 111 63 90 144 1269 108 225 450
Cuts at: 4657 4684 4891 2197
Size: 27 207 6109
Fragments arranged by size:
6109 1269 450 225 207 144 111 108
90 63 27
SfcI C'TryA_G
Cuts at: 826 1045 2388 4044 4881 5353 5879 6151 7286
Size: 219 1343 1656 837 472 526 272 1135
Cuts at: 7286 7967 8158 826
Size: 681 191 1471
Fragments arranged by size:
1656 1471 1343 1135 837 681 526 472
272 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2877 3444 5271 2877
Size: 567 1827 6409
Fragments arranged by size:
6409 1827 567
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8803
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8803
SmaI CCC'GGG
Cuts at: 1872 6130 1872
Size: 4258 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2334 4636 5500 6907 7808 8070
Size: 197 926 391 2302 864 1407 901 262
Cuts at: 8070 820
Size: 1553
Fragments arranged by size:
2302 1553 1407 926 901 864 391 262
197
SnaBI TAC'GTA
Cuts at: 493 6594 493
Size: 6101 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8803
SphI G_CATG'C
Cuts at: 1132 6161 1132
Size: 5029 3774
SspI AAT'ATT
Cuts at: 5 52 4864 6727 5
Size: 47 4812 1863 2081
Fragments arranged by size:
4812 2081 1863 47
StuI AGG'CCT
Cuts at: 1517 2871 3433 1517
Size: 1354 562 6887
Fragments arranged by size:
6887 1354 562
StyI C'CwwG_G
Cuts at: 513 1065 2344 2793 2928 3109 3730 4477 5116
Size: 552 1279 449 135 181 621 747 639
Cuts at: 5116 5159 5260 5394 5554 5664 5745 6242 513
Size: 43 101 134 160 110 81 497 3074
Fragments arranged by size:
3074 1279 747 639 621 552 497 449
181 160 135 134 110 101 81 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4145 4667 5529 6830 6847 7000 7185
Size: 1255 2565 522 862 1301 17 153 185
Cuts at: 7185 7630 325
Size: 445 1498
Fragments arranged by size:
2565 1498 1301 1255 862 522 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 7049 8761 96
Size: 274 80 33 51 1443 5072 1712 138
Fragments arranged by size:
5072 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2170 2202 3496 3528 3889 3921 4573 4605 5197
Size: 32 1294 32 361 32 652 32 592
Cuts at: 5197 5229 8763 8795 2170
Size: 32 3534 32 2178
Fragments arranged by size:
3534 2178 1294 652 592 361 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8803
XbaI T'CTAG_A
Cuts at: 5468 6139 5468
Size: 671 8132
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2351 3116 3674 3737 4484 5267 5646 2351
Size: 765 558 63 747 783 379 5508
Fragments arranged by size:
5508 783 765 747 558 379 63
XhoI C'TCGA_G
Cuts at: 1943 4636 1943
Size: 2693 6110
XmaI C'CCGG_G
Cuts at: 1870 6128 1870
Size: 4258 4545
XmnI GAAnn'nnTTC
Cuts at: 4455 6932 4455
Size: 2477 6326
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6611 276
Size: 53 83 186 936 5077 2468
Fragments arranged by size:
5077 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NruI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SacI SacII SalI SanDI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BfrBI BsiWI BsmBI
EcoRV FseI FspAI KasI NarI NsiI PacI PspXI
RsrII SapI SfoI SrfI SwaI