Restriction map for
pFN21AB4714
(Circular) (Six-base) MAPSORT of: pFN21AB4714.seq Check: 4547 from: 1 to: 7327
pFN21AB4714 7327 bp
With 182 enzymes: SgfI *
October 20, 2009 10:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2508 3461 4558 2508
Size: 953 1097 5277
Fragments arranged by size:
5277 1097 953
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5137 278
Size: 53 83 186 936 3601 2468
Fragments arranged by size:
3601 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4379 4602 4670 1937
Size: 2442 223 68 4594
Fragments arranged by size:
4594 2442 223 68
Acc65I G'GTAC_C
Cuts at: 4648 4648
Size: 7327
AclI AA'CG_TT
Cuts at: 1563 4367 5454 5827 1563
Size: 2804 1087 373 3063
Fragments arranged by size:
3063 2804 1087 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2525 3563 5390 6774 7042 1632
Size: 893 1038 1827 1384 268 1917
Fragments arranged by size:
1917 1827 1384 1038 893 268
AfeI AGC'GCT
Cuts at: 2429 2531 2429
Size: 102 7225
AflII C'TTAA_G
Cuts at: 820 1017 2672 3050 820
Size: 197 1655 378 5097
Fragments arranged by size:
5097 1655 378 197
AflIII A'CryG_T
Cuts at: 1203 4482 6226 7203 1203
Size: 3279 1744 977 1327
Fragments arranged by size:
3279 1744 1327 977
AgeI A'CCGG_T
Cuts at: 6158 6158
Size: 7327
AhdI GACnn_n'nnGTC
Cuts at: 3733 6056 3733
Size: 2323 5004
AleI CACnn'nnGTG
Cuts at: 1166 3319 4130 4160 4440 1166
Size: 2153 811 30 280 4053
Fragments arranged by size:
4053 2153 811 280 30
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2429 1184
Size: 1245 6082
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3329 3361 4352 4384 3329
Size: 32 991 32 6272
Fragments arranged by size:
6272 991 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3476 5070 6642 3476
Size: 1594 1572 4161
Fragments arranged by size:
4161 1594 1572
ApaI G_GGCC'C
Cuts at: 2372 3396 3888 2372
Size: 1024 492 5811
Fragments arranged by size:
5811 1024 492
ApaLI G'TGCA_C
Cuts at: 5384 6540 5384
Size: 1156 6171
ApoI r'AATT_y
Cuts at: 1493 2015 3125 4349 4636 4862 5014 7123 1493
Size: 522 1110 1224 287 226 152 2109 1697
Fragments arranged by size:
2109 1697 1224 1110 522 287 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5881 160
Size: 872 4849 1606
Fragments arranged by size:
4849 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7327
AvaI C'yCGr_G
Cuts at: 1870 1943 4112 4652 1870
Size: 73 2169 540 4545
Fragments arranged by size:
4545 2169 540 73
BamHI G'GATC_C
Cuts at: 4657 5024 4657
Size: 367 6960
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2458 3754 4648 6103 618
Size: 325 217 627 671 1296 894 1455 1842
Fragments arranged by size:
1842 1455 1296 894 671 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2372 2386 2601 3396 3505 3688 3888 3912
Size: 981 14 215 795 109 183 200 24
Cuts at: 3912 4053 4646 1391
Size: 141 593 4072
Fragments arranged by size:
4072 981 795 593 215 200 183 141
109 24 14
BbeI G_GCGC'C
Cuts at: 3758 3758
Size: 7327
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2361 2481 4155 928
Size: 971 462 120 1674 4100
Fragments arranged by size:
4100 1674 971 462 120
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2370 2404 5518 5552 1903
Size: 34 433 34 3114 34 3678
Fragments arranged by size:
3678 3114 433 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5219 6429 5219
Size: 1210 6117
BclI T'GATC_A
Cuts at: 1548 2646 4288 1548
Size: 1098 1642 4587
Fragments arranged by size:
4587 1642 1098
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2819 3032 5938
Size: 107 122 71 631 605 1147 213 2906
Cuts at: 5938 136
Size: 1525
Fragments arranged by size:
2906 1525 1147 631 605 213 122 107
71
BglII A'GATC_T
Cuts at: 3093 3837 4267 7322 3093
Size: 744 430 3055 3098
Fragments arranged by size:
3098 3055 744 430
BlpI GC'TnA_GC
Cuts at: 4744 4744
Size: 7327
Bme1580I G_kGCm'C
Cuts at: 1790 2140 2372 2463 3160 3396 3744 3888 5388
Size: 350 232 91 697 236 348 144 1500
Cuts at: 5388 6544 1790
Size: 1156 2573
Fragments arranged by size:
2573 1500 1156 697 350 348 236 232
144 91
BmgBI CAC'GTC
Cuts at: 1318 2521 1318
Size: 1203 6124
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2355 2457 3519
Size: 416 53 83 399 24 938 102 1062
Cuts at: 3519 4230 5058 6016 442
Size: 711 828 958 1753
Fragments arranged by size:
1753 1062 958 938 828 711 416 399
102 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7327
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2072 2455 2864 3011 3629 3698 5987 1239
Size: 833 383 409 147 618 69 2289 2579
Fragments arranged by size:
2579 2289 833 618 409 383 147 69
Bpu10I CC'TnA_GC
Cuts at: 2547 3906 2547
Size: 1359 5968
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2090 3716 5452 6317 6615 2090
Size: 1626 1736 865 298 2802
Fragments arranged by size:
2802 1736 1626 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5990 882
Size: 617 4491 2219
Fragments arranged by size:
4491 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4081 5118 493
Size: 711 2877 1037 2702
Fragments arranged by size:
2877 2702 1037 711
BsaBI GATnn'nnATC
Cuts at: 7321 7321
Size: 7327
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3755 5134 5516 6151
Size: 53 83 186 936 2222 1379 382 635
Cuts at: 6151 275
Size: 1451
Fragments arranged by size:
2222 1451 1379 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5760 6158 6432 6579 6990 5760
Size: 398 274 147 411 6097
Fragments arranged by size:
6097 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2583 2613 4352 4382 1178
Size: 30 165 30 1180 30 1739 30 4123
Fragments arranged by size:
4123 1739 1180 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 2744 3088 3473 4604 1186
Size: 862 696 344 385 1131 3909
Fragments arranged by size:
3909 1131 862 696 385 344
BseYI C'CCAG_C
Cuts at: 2532 6530 7026 2532
Size: 3998 496 2833
Fragments arranged by size:
3998 2833 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2530 3937 3982 4061 4153 1163
Size: 573 794 1407 45 79 92 4337
Fragments arranged by size:
4337 1407 794 573 92 79 45
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2205 2376 3079 4777 5538 6566
Size: 275 186 204 171 703 1698 761 1028
Cuts at: 6566 1540
Size: 2301
Fragments arranged by size:
2301 1698 1028 761 703 275 204 186
171
BsiHKAI G_wGCw'C
Cuts at: 2184 2880 3487 4074 5388 5473 6544 2184
Size: 696 607 587 1314 85 1071 2967
Fragments arranged by size:
2967 1314 1071 696 607 587 85
BsmI GAATG_Cn'
Cuts at: 4437 4838 4931 4437
Size: 401 93 6833
Fragments arranged by size:
6833 401 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3926 3926
Size: 7327
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2140 2184 2372 2386 2463 2601 2880
Size: 399 350 44 188 14 77 138 279
Cuts at: 2880 3160 3396 3487 3505 3688 3744 3888 3912
Size: 280 236 91 18 183 56 144 24
Cuts at: 3912 4053 4074 4646 5388 5473 6544 1391
Size: 141 21 572 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 572 399 350 280 279
236 188 183 144 141 138 91 85
77 56 44 24 21 18 14
BspEI T'CCGG_A
Cuts at: 6990 6990
Size: 7327
BspHI T'CATG_A
Cuts at: 3084 4305 4516 5216 6946 3084
Size: 1221 211 700 1730 3465
Fragments arranged by size:
3465 1730 1221 700 211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2508 3461 4127 4558 4682 844
Size: 1664 953 666 431 124 3489
Fragments arranged by size:
3489 1664 953 666 431 124
BsrBI CCG'CTC
Cuts at: 1394 1823 5214 1394
Size: 429 3391 3507
Fragments arranged by size:
3507 3391 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2295 3021 5822 5996 65
Size: 1183 1047 726 2801 174 1396
Fragments arranged by size:
2801 1396 1183 1047 726 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5971 6158 1700
Size: 110 4161 187 2869
Fragments arranged by size:
4161 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7327
BssHII G'CGCG_C
Cuts at: 1925 3247 1925
Size: 1322 6005
BssSI C'ACGA_G
Cuts at: 2483 2792 5387 6399 2483
Size: 309 2595 1012 3411
Fragments arranged by size:
3411 2595 1012 309
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7327
BstBI TT'CG_AA
Cuts at: 4320 6131 4320
Size: 1811 5516
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7327
BstXI CCAn_nnnn'nTGG
Cuts at: 2857 3532 3979 4162 4585 2857
Size: 675 447 183 423 5599
Fragments arranged by size:
5599 675 447 423 183
BstYI r'GATC_y
Cuts at: 1972 2986 3093 3837 4267 4657 5024 5409 5426
Size: 1014 107 744 430 390 367 385 17
Cuts at: 5426 6878 6964 6976 7322 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1014 744 430 390 385 367
346 107 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2193 3750 3759 3874 3912 513
Size: 552 1128 1557 9 115 38 3928
Fragments arranged by size:
3928 1557 1128 552 115 38 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6588
Fragments arranged by size:
6588 683 56
BtsI GCAGTG_nn'
Cuts at: 2851 3867 4136 4843 5637 5657 2851
Size: 1016 269 707 794 20 4521
Fragments arranged by size:
4521 1016 794 707 269 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7327
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7292
DraI TTT'AAA
Cuts at: 4631 4631
Size: 7327
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3310 6334 809
Size: 507 1994 3024 1802
Fragments arranged by size:
3024 1994 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2373 2709 2820
Size: 54 1425 50 275 18 543 336 111
Cuts at: 2820 3033 3357 3747 4774 5663 7292 8
Size: 213 324 390 1027 889 1629 43
Fragments arranged by size:
1629 1425 1027 889 543 390 336 324
275 213 111 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2373 4774 1537
Size: 275 561 2401 4090
Fragments arranged by size:
4090 2401 561 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2069 2721 3219 3276 3507 3539 3896 4392
Size: 716 652 498 57 231 32 357 496
Cuts at: 4392 5257 1353
Size: 865 3423
Fragments arranged by size:
3423 865 716 652 498 496 357 231
57 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5914 6288 6434 387
Size: 1064 138 4325 374 146 1280
Fragments arranged by size:
4325 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2455 2525 2864 3011 3563 3629
Size: 393 440 383 70 339 147 552 66
Cuts at: 3629 3698 5390 5987 6774 7042 1239
Size: 69 1692 597 787 268 1524
Fragments arranged by size:
1692 1524 787 597 552 440 393 383
339 268 147 70 69 66
EcoNI CCTnn'n_nnAGG
Cuts at: 2939 3383 2939
Size: 444 6883
EcoO109I rG'GnC_Cy
Cuts at: 2368 3392 3625 3885 4116 2368
Size: 1024 233 260 231 5579
Fragments arranged by size:
5579 1024 260 233 231
EcoRI G'AATT_C
Cuts at: 3125 4636 5014 3125
Size: 1511 378 5438
Fragments arranged by size:
5438 1511 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2530 2698 2530
Size: 168 7159
FspI TGC'GCA
Cuts at: 2783 5833 2783
Size: 3050 4277
HaeII r_GCGC'y
Cuts at: 1678 2431 2533 3758 6474 1678
Size: 753 102 1225 2716 2531
Fragments arranged by size:
2716 2531 1225 753 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2021 2053 2146 2178 2543
Size: 32 135 32 449 32 93 32 365
Cuts at: 2543 2575 3391 3423 3719 3751 5968 6000 6042
Size: 32 816 32 296 32 2217 32 42
Cuts at: 6042 6074 1373
Size: 32 2626
Fragments arranged by size:
2626 2217 816 449 365 296 135 93
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4671 4917 669
Size: 988 281 2733 246 3079
Fragments arranged by size:
3079 2733 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7327
HpaI GTT'AAC
Cuts at: 4917 4917
Size: 7327
KasI G'GCGC_C
Cuts at: 3754 3754
Size: 7327
KpnI G_GTAC'C
Cuts at: 4652 4652
Size: 7327
MfeI C'AATT_G
Cuts at: 4926 4926
Size: 7327
MluI A'CGCG_T
Cuts at: 7203 7203
Size: 7327
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2076 2240 6441 6625 1305
Size: 771 164 4201 184 2007
Fragments arranged by size:
4201 2007 771 184 164
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2711 3035 3359 3749 10
Size: 54 1425 343 879 324 324 390 3588
Fragments arranged by size:
3588 1425 879 390 343 324 324 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3319 4130 4160 4440 4526 5285 5644
Size: 648 2153 811 30 280 86 759 359
Cuts at: 5644 5803 518
Size: 159 2042
Fragments arranged by size:
2153 2042 811 759 648 359 280 159
86 30
MspA1I CmG'CkG
Cuts at: 1606 2722 2966 3876 3951 4160 4179 4743 5420
Size: 1116 244 910 75 209 19 564 677
Cuts at: 5420 6568 6813 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1116 910 677 564 245 244
209 75 19
NarI GG'CG_CC
Cuts at: 3755 3755
Size: 7327
NcoI C'CATG_G
Cuts at: 513 1065 3759 513
Size: 552 2694 4081
Fragments arranged by size:
4081 2694 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7327
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7327
NotI GC'GGCC_GC
Cuts at: 4774 4774
Size: 7327
NspI r_CATG'y
Cuts at: 1132 1229 4685 6230 1132
Size: 97 3456 1545 2229
Fragments arranged by size:
3456 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7327
PciI A'CATG_T
Cuts at: 6226 6226
Size: 7327
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4024 1108
Size: 2916 4411
PfoI T'CCnGG_A
Cuts at: 2088 3606 3843 2088
Size: 1518 237 5572
Fragments arranged by size:
5572 1518 237
PmeI GTTT'AAAC
Cuts at: 4631 4631
Size: 7327
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4352 4384 5396 5428 6909 6941 4352
Size: 32 1012 32 1481 32 4738
Fragments arranged by size:
4738 1481 1012 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4116 4116
Size: 7327
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7327
PsiI TTA'TAA
Cuts at: 4897 4897
Size: 7327
PspOMI G'GGCC_C
Cuts at: 2368 3392 3884 2368
Size: 1024 492 5811
Fragments arranged by size:
5811 1024 492
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3766 3798 3766
Size: 32 7295
PstI C_TGCA'G
Cuts at: 830 2418 2846 2894 3693 4679 830
Size: 1588 428 48 799 986 3478
Fragments arranged by size:
3478 1588 986 799 428 48
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7327
PvuII CAG'CTG
Cuts at: 2722 2966 4160 4179 2722
Size: 244 1194 19 5870
Fragments arranged by size:
5870 1194 244 19
SacII CC_GC'GG
Cuts at: 3877 3877
Size: 7327
SalI G'TCGA_C
Cuts at: 1936 4669 1936
Size: 2733 4594
SbfI CC_TGCA'GG
Cuts at: 4679 4679
Size: 7327
ScaI AGT'ACT
Cuts at: 5575 7287 5575
Size: 1712 5615
SexAI A'CCwGG_T
Cuts at: 2997 2997
Size: 7327
SfcI C'TryA_G
Cuts at: 826 1045 2414 2842 2890 3689 4281 4675 5810
Size: 219 1369 428 48 799 592 394 1135
Cuts at: 5810 6491 6682 826
Size: 681 191 1471
Fragments arranged by size:
1471 1369 1135 799 681 592 428 394
219 191 48
SfoI GGC'GCC
Cuts at: 3756 3756
Size: 7327
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7327
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7327
SmaI CCC'GGG
Cuts at: 1872 4114 4654 1872
Size: 2242 540 4545
Fragments arranged by size:
4545 2242 540
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2069 2672 3050 3731 5431 6332
Size: 197 926 126 603 378 681 1700 901
Cuts at: 6332 6594 820
Size: 262 1553
Fragments arranged by size:
1700 1553 926 901 681 603 378 262
197 126
SnaBI TAC'GTA
Cuts at: 493 5118 493
Size: 4625 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7327
SphI G_CATG'C
Cuts at: 1132 4685 1132
Size: 3553 3774
SspI AAT'ATT
Cuts at: 5 52 4414 5251 5
Size: 47 4362 837 2081
Fragments arranged by size:
4362 2081 837 47
StuI AGG'CCT
Cuts at: 1517 2210 4061 1517
Size: 693 1851 4783
Fragments arranged by size:
4783 1851 693
StyI C'CwwG_G
Cuts at: 513 1065 2901 3759 4023 4766 513
Size: 552 1836 858 264 743 3074
Fragments arranged by size:
3074 1836 858 743 552 264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2219 2524 2914 3597 4493 5354 5371
Size: 1255 639 305 390 683 896 861 17
Cuts at: 5371 5524 5709 6154 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 896 861 683 639 445 390
305 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3417 3696 5573
Size: 274 80 33 51 1443 1440 279 1877
Cuts at: 5573 7285 96
Size: 1712 138
Fragments arranged by size:
1877 1712 1443 1440 279 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2583 2615 7287 7319 2583
Size: 32 4672 32 2591
Fragments arranged by size:
4672 2591 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2522 3661 1316
Size: 1206 1139 4982
Fragments arranged by size:
4982 1206 1139
XbaI T'CTAG_A
Cuts at: 3703 4663 3703
Size: 960 6367
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2719 3673 3757 2719
Size: 954 84 6289
Fragments arranged by size:
6289 954 84
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7327
XmaI C'CCGG_G
Cuts at: 1870 4112 4652 1870
Size: 2242 540 4545
Fragments arranged by size:
4545 2242 540
XmnI GAAnn'nnTTC
Cuts at: 2015 5456 2015
Size: 3441 3886
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5135 276
Size: 53 83 186 936 3601 2468
Fragments arranged by size:
3601 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacII SalI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PspXI RsrII SacI SanDI
SapI SfiI SrfI SwaI