Restriction map for
pFN21AB4708
(Circular) (Six-base) MAPSORT of: pFN21AB4708.seq Check: 4932 from: 1 to: 6037
pFN21AB4708 6037 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3101 3101
Size: 6037
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3847 278
Size: 53 83 186 936 2311 2468
Fragments arranged by size:
2468 2311 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2565 3380 1937
Size: 628 815 4594
Fragments arranged by size:
4594 815 628
Acc65I G'GTAC_C
Cuts at: 3358 3358
Size: 6037
AclI AA'CG_TT
Cuts at: 1563 4164 4537 1563
Size: 2601 373 3063
Fragments arranged by size:
3063 2601 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2050 3314 4100 5484 5752 1632
Size: 418 1264 786 1384 268 1917
Fragments arranged by size:
1917 1384 1264 786 418 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5840
AflIII A'CryG_T
Cuts at: 1203 2394 2715 4936 5913 1203
Size: 1191 321 2221 977 1327
Fragments arranged by size:
2221 1327 1191 977 321
AgeI A'CCGG_T
Cuts at: 4868 4868
Size: 6037
AhdI GACnn_n'nnGTC
Cuts at: 4766 4766
Size: 6037
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6037
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6037
AlwNI CAG_nnn'CTG
Cuts at: 3780 5352 3780
Size: 1572 4465
ApaI G_GGCC'C
Cuts at: 2350 3271 2350
Size: 921 5116
ApaLI G'TGCA_C
Cuts at: 4094 5250 4094
Size: 1156 4881
ApoI r'AATT_y
Cuts at: 1493 2243 2600 3346 3572 3724 5833 1493
Size: 750 357 746 226 152 2109 1697
Fragments arranged by size:
2109 1697 750 746 357 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4591 160
Size: 872 3559 1606
Fragments arranged by size:
3559 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6037
AvaI C'yCGr_G
Cuts at: 1870 1943 2164 3362 1870
Size: 73 221 1198 4545
Fragments arranged by size:
4545 1198 221 73
BamHI G'GATC_C
Cuts at: 2692 3367 3734 2692
Size: 675 367 4995
Fragments arranged by size:
4995 675 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2056 2624 3358 4813 618
Size: 325 217 627 269 568 734 1455 1842
Fragments arranged by size:
1842 1455 734 627 568 325 269 217
BanII G_rGCy'C
Cuts at: 1391 2301 2350 2962 3271 3356 1391
Size: 910 49 612 309 85 4072
Fragments arranged by size:
4072 910 612 309 85 49
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2363 928
Size: 971 464 4602
Fragments arranged by size:
4602 971 464
BbvCI CC'TCA_GC
Cuts at: 2364 2364
Size: 6037
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4228 4262 1903
Size: 34 2291 34 3678
Fragments arranged by size:
3678 2291 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3929 5139 3929
Size: 1210 4827
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6037
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4648 136
Size: 107 122 71 631 605 2976 1525
Fragments arranged by size:
2976 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6032 6032
Size: 6037
BlpI GC'TnA_GC
Cuts at: 3454 3454
Size: 6037
Bme1580I G_kGCm'C
Cuts at: 1790 2061 2190 2350 3271 4098 5254 1790
Size: 271 129 160 921 827 1156 2573
Fragments arranged by size:
2573 1156 921 827 271 160 129
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6037
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2890 3768 4726
Size: 416 53 83 399 24 1473 878 958
Cuts at: 4726 442
Size: 1753
Fragments arranged by size:
1753 1473 958 878 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6037
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2217 2453 3011 4697 1239
Size: 978 236 558 1686 2579
Fragments arranged by size:
2579 1686 978 558 236
Bpu10I CC'TnA_GC
Cuts at: 2364 2364
Size: 6037
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4162 5027 5325 4162
Size: 865 298 4874
Fragments arranged by size:
4874 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4700 882
Size: 617 3201 2219
Fragments arranged by size:
3201 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2395 2716 3828 493
Size: 711 1191 321 1112 2702
Fragments arranged by size:
2702 1191 1112 711 321
BsaBI GATnn'nnATC
Cuts at: 6031 6031
Size: 6037
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3321 3844 4226 4861
Size: 53 83 186 936 1788 523 382 635
Cuts at: 4861 275
Size: 1451
Fragments arranged by size:
1788 1451 936 635 523 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2916 4470 4868 5142 5289 5700 2916
Size: 1554 398 274 147 411 3253
Fragments arranged by size:
3253 1554 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5812
Fragments arranged by size:
5812 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2090 2309 2396 2399 1186
Size: 904 219 87 3 4824
Fragments arranged by size:
4824 904 219 87 3
BseYI C'CCAG_C
Cuts at: 2070 2300 5240 5736 2070
Size: 230 2940 496 2371
Fragments arranged by size:
2940 2371 496 230
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2696 1163
Size: 573 960 4504
Fragments arranged by size:
4504 960 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3487 4248 5276 1540
Size: 275 186 1486 761 1028 2301
Fragments arranged by size:
2301 1486 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3159 4098 4183 5254 3159
Size: 939 85 1071 3942
Fragments arranged by size:
3942 1071 939 85
BsmI GAATG_Cn'
Cuts at: 3548 3641 3548
Size: 93 5944
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2190 2301 2350 2962 3159 3271
Size: 399 271 129 111 49 612 197 112
Cuts at: 3271 3356 4098 4183 5254 1391
Size: 85 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 612 399 271 197 129
112 111 85 85 49
BspEI T'CCGG_A
Cuts at: 5700 5700
Size: 6037
BspHI T'CATG_A
Cuts at: 2856 3926 5656 2856
Size: 1070 1730 3237
Fragments arranged by size:
3237 1730 1070
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2103 2781 3101 3392 844
Size: 1259 678 320 291 3489
Fragments arranged by size:
3489 1259 678 320 291
BsrBI CCG'CTC
Cuts at: 1394 1823 3924 1394
Size: 429 2101 3507
Fragments arranged by size:
3507 2101 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2439 2476 4532 4706 65
Size: 1183 1191 37 2056 174 1396
Fragments arranged by size:
2056 1396 1191 1183 174 37
BsrFI r'CCGG_y
Cuts at: 1700 1810 4681 4868 1700
Size: 110 2871 187 2869
Fragments arranged by size:
2871 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6037
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6037
BssSI C'ACGA_G
Cuts at: 4097 5109 4097
Size: 1012 5025
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2095 2213 794
Size: 1301 118 4618
Fragments arranged by size:
4618 1301 118
BstBI TT'CG_AA
Cuts at: 4841 4841
Size: 6037
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6037
BstYI r'GATC_y
Cuts at: 1972 2692 3367 3734 4119 4136 5588 5674 5686
Size: 720 675 367 385 17 1452 86 12
Cuts at: 5686 6032 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 720 675 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2351 2351
Size: 6037
BtgI C'CryG_G
Cuts at: 513 1065 2004 2311 513
Size: 552 939 307 4239
Fragments arranged by size:
4239 939 552 307
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2410 2633 487
Size: 683 56 1184 223 3891
Fragments arranged by size:
3891 1184 683 223 56
BtsI GCAGTG_nn'
Cuts at: 3553 4347 4367 3553
Size: 794 20 5223
Fragments arranged by size:
5223 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6037
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6002
DraI TTT'AAA
Cuts at: 3341 3341
Size: 6037
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5044 809
Size: 507 3728 1802
Fragments arranged by size:
3728 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2551 2611 3484
Size: 54 1425 50 275 18 721 60 873
Cuts at: 3484 4373 6002 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 873 721 275 60 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3484 1537
Size: 275 1672 4090
Fragments arranged by size:
4090 1672 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3170 3967 1353
Size: 1817 797 3423
Fragments arranged by size:
3423 1817 797
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4624 4998 5144 387
Size: 1064 138 3035 374 146 1280
Fragments arranged by size:
3035 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2050 2217 2453 3011 3314 4100 4697
Size: 393 418 167 236 558 303 786 597
Cuts at: 4697 5484 5752 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 786 597 558 418 393 303
268 236 167
EcoO109I rG'GnC_Cy
Cuts at: 2347 3267 3268 3288 2347
Size: 920 1 20 5096
Fragments arranged by size:
5096 920 20 1
EcoRI G'AATT_C
Cuts at: 2243 3346 3724 2243
Size: 1103 378 4556
Fragments arranged by size:
4556 1103 378
FspI TGC'GCA
Cuts at: 2099 4543 2099
Size: 2444 3593
HaeII r_GCGC'y
Cuts at: 1678 5184 1678
Size: 3506 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4678 4710 4752 4784 1373
Size: 32 135 32 3106 32 42 32 2626
Fragments arranged by size:
3106 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3381 3627 669
Size: 988 281 1443 246 3079
Fragments arranged by size:
3079 1443 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6037
HpaI GTT'AAC
Cuts at: 3627 3627
Size: 6037
KpnI G_GTAC'C
Cuts at: 3362 3362
Size: 6037
MfeI C'AATT_G
Cuts at: 3636 3636
Size: 6037
MluI A'CGCG_T
Cuts at: 5913 5913
Size: 6037
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5151 5335 1305
Size: 3846 184 2007
Fragments arranged by size:
3846 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2553 2613 10
Size: 54 1425 343 721 60 3434
Fragments arranged by size:
3434 1425 721 343 60 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2547 2831 2861 3020 3035 3995 4354
Size: 648 1381 284 30 159 15 960 359
Cuts at: 4354 4513 518
Size: 159 2042
Fragments arranged by size:
2042 1381 960 648 359 284 159 159
30 15
MspA1I CmG'CkG
Cuts at: 1606 2070 2176 2821 3082 3208 3453 4130 5278
Size: 464 106 645 261 126 245 677 1148
Cuts at: 5278 5523 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 645 464 261 245 245
126 106
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4546
Fragments arranged by size:
4546 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6037
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6037
NotI GC'GGCC_GC
Cuts at: 3484 3484
Size: 6037
NspI r_CATG'y
Cuts at: 1132 1229 2710 3395 4940 1132
Size: 97 1481 685 1545 2229
Fragments arranged by size:
2229 1545 1481 685 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6037
PciI A'CATG_T
Cuts at: 4936 4936
Size: 6037
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2640 1108
Size: 1532 4505
PfoI T'CCnGG_A
Cuts at: 2988 2988
Size: 6037
PmeI GTTT'AAAC
Cuts at: 3341 3341
Size: 6037
PmlI CAC'GTG
Cuts at: 2395 2716 2395
Size: 321 5716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4106 4138 5619 5651 4106
Size: 32 1481 32 4492
Fragments arranged by size:
4492 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3288 3288
Size: 6037
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6037
PsiI TTA'TAA
Cuts at: 3607 3607
Size: 6037
PspOMI G'GGCC_C
Cuts at: 2346 3267 2346
Size: 921 5116
PstI C_TGCA'G
Cuts at: 830 3389 830
Size: 2559 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6037
PvuII CAG'CTG
Cuts at: 2070 3082 2070
Size: 1012 5025
SalI G'TCGA_C
Cuts at: 1936 3379 1936
Size: 1443 4594
SbfI CC_TGCA'GG
Cuts at: 3389 3389
Size: 6037
ScaI AGT'ACT
Cuts at: 4285 5997 4285
Size: 1712 4325
SfcI C'TryA_G
Cuts at: 826 1045 2566 3385 4520 5201 5392 826
Size: 219 1521 819 1135 681 191 1471
Fragments arranged by size:
1521 1471 1135 819 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6037
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6037
SmaI CCC'GGG
Cuts at: 1872 2166 3364 1872
Size: 294 1198 4545
Fragments arranged by size:
4545 1198 294
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4141 5042 5304 820
Size: 197 926 2198 901 262 1553
Fragments arranged by size:
2198 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3828 493
Size: 3335 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6037
SphI G_CATG'C
Cuts at: 1132 3395 1132
Size: 2263 3774
SspI AAT'ATT
Cuts at: 5 52 3961 5
Size: 47 3909 2081
Fragments arranged by size:
3909 2081 47
StuI AGG'CCT
Cuts at: 1517 3056 1517
Size: 1539 4498
StyI C'CwwG_G
Cuts at: 513 1065 2004 3476 513
Size: 552 939 1472 3074
Fragments arranged by size:
3074 1472 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2872 4064 4081 4234 4419 4864 325
Size: 1255 1292 1192 17 153 185 445 1498
Fragments arranged by size:
1498 1292 1255 1192 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4283 5995 96
Size: 274 80 33 51 1443 2306 1712 138
Fragments arranged by size:
2306 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5997 6029 5997
Size: 32 6005
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6037
XbaI T'CTAG_A
Cuts at: 3373 3373
Size: 6037
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3097 3097
Size: 6037
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6037
XmaI C'CCGG_G
Cuts at: 1870 2164 3362 1870
Size: 294 1198 4545
Fragments arranged by size:
4545 1198 294
XmnI GAAnn'nnTTC
Cuts at: 2378 4166 2378
Size: 1788 4249
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3845 276
Size: 53 83 186 936 2311 2468
Fragments arranged by size:
2468 2311 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI