Restriction map for
pFN21AB4707
(Circular) (Six-base) MAPSORT of: pFN21AB4707.seq Check: 4554 from: 1 to: 5791
pFN21AB4707 5791 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2184 3601 278
Size: 53 83 186 936 648 1417 2468
Fragments arranged by size:
2468 1417 936 648 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3134 1937
Size: 1197 4594
Acc65I G'GTAC_C
Cuts at: 3112 3112
Size: 5791
AclI AA'CG_TT
Cuts at: 1563 3918 4291 1563
Size: 2355 373 3063
Fragments arranged by size:
3063 2355 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2576 2595 2948 3854 5238 5506 1632
Size: 944 19 353 906 1384 268 1917
Fragments arranged by size:
1917 1384 944 906 353 268 19
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5594
AflIII A'CryG_T
Cuts at: 1203 2419 4690 5667 1203
Size: 1216 2271 977 1327
Fragments arranged by size:
2271 1327 1216 977
AgeI A'CCGG_T
Cuts at: 4622 4622
Size: 5791
AhdI GACnn_n'nnGTC
Cuts at: 4520 4520
Size: 5791
AleI CACnn'nnGTG
Cuts at: 1166 2033 2512 1166
Size: 867 479 4445
Fragments arranged by size:
4445 867 479
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5791
AlwNI CAG_nnn'CTG
Cuts at: 2281 3534 5106 2281
Size: 1253 1572 2966
Fragments arranged by size:
2966 1572 1253
ApaLI G'TGCA_C
Cuts at: 3848 5004 3848
Size: 1156 4635
ApoI r'AATT_y
Cuts at: 1493 3100 3326 3478 5587 1493
Size: 1607 226 152 2109 1697
Fragments arranged by size:
2109 1697 1607 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4345 160
Size: 872 3313 1606
Fragments arranged by size:
3313 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5791
AvaI C'yCGr_G
Cuts at: 1870 1943 2434 3116 1870
Size: 73 491 682 4545
Fragments arranged by size:
4545 682 491 73
BamHI G'GATC_C
Cuts at: 3121 3488 3121
Size: 367 5424
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3112 4567 618
Size: 325 217 627 1325 1455 1842
Fragments arranged by size:
1842 1455 1325 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2367 2655 3110 1391
Size: 976 288 455 4072
Fragments arranged by size:
4072 976 455 288
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2216 928
Size: 971 317 4503
Fragments arranged by size:
4503 971 317
BbvCI CC'TCA_GC
Cuts at: 2635 2635
Size: 5791
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3982 4016 1903
Size: 34 2045 34 3678
Fragments arranged by size:
3678 2045 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2630 3683 4893 2630
Size: 1053 1210 3528
Fragments arranged by size:
3528 1210 1053
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5791
BfrBI ATG'CAT
Cuts at: 2129 2129
Size: 5791
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4402 136
Size: 107 122 71 631 605 2730 1525
Fragments arranged by size:
2730 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5786 5786
Size: 5791
BlpI GC'TnA_GC
Cuts at: 3208 3208
Size: 5791
Bme1580I G_kGCm'C
Cuts at: 1790 3852 5008 1790
Size: 2062 1156 2573
Fragments arranged by size:
2573 2062 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5791
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3522 4480 442
Size: 416 53 83 399 24 2105 958 1753
Fragments arranged by size:
2105 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5791
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2669 4451 1239
Size: 1430 1782 2579
Fragments arranged by size:
2579 1782 1430
Bpu10I CC'TnA_GC
Cuts at: 2635 2635
Size: 5791
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2125 2826 2982 3916 4781 5079 2125
Size: 701 156 934 865 298 2837
Fragments arranged by size:
2837 934 865 701 298 156
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2818 4454 882
Size: 617 1319 1636 2219
Fragments arranged by size:
2219 1636 1319 617
BsaAI yAC'GTr
Cuts at: 493 1204 2420 3582 493
Size: 711 1216 1162 2702
Fragments arranged by size:
2702 1216 1162 711
BsaBI GATnn'nnATC
Cuts at: 5785 5785
Size: 5791
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2181 3598 3980 4615
Size: 53 83 186 936 648 1417 382 635
Cuts at: 4615 275
Size: 1451
Fragments arranged by size:
1451 1417 936 648 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4224 4622 4896 5043 5454 4224
Size: 398 274 147 411 4561
Fragments arranged by size:
4561 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5566
Fragments arranged by size:
5566 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2777 2910 1186
Size: 1591 133 4067
Fragments arranged by size:
4067 1591 133
BseYI C'CCAG_C
Cuts at: 4994 5490 4994
Size: 496 5295
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5218
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3241 4002 5030 1540
Size: 275 186 1240 761 1028 2301
Fragments arranged by size:
2301 1240 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2655 3852 3937 5008 2655
Size: 1197 85 1071 3438
Fragments arranged by size:
3438 1197 1071 85
BsmI GAATG_Cn'
Cuts at: 2842 3302 3395 2842
Size: 460 93 5238
Fragments arranged by size:
5238 460 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2367 2655 3110 3852 3937 5008 1391
Size: 399 577 288 455 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 577 455 399 288 85
BspEI T'CCGG_A
Cuts at: 5454 5454
Size: 5791
BspHI T'CATG_A
Cuts at: 3680 5410 3680
Size: 1730 4061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2836 3146 844
Size: 1992 310 3489
Fragments arranged by size:
3489 1992 310
BsrBI CCG'CTC
Cuts at: 1394 1823 3678 1394
Size: 429 1855 3507
Fragments arranged by size:
3507 1855 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4286 4460 65
Size: 1183 3038 174 1396
Fragments arranged by size:
3038 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4435 4622 1700
Size: 110 2625 187 2869
Fragments arranged by size:
2869 2625 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5791
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5791
BssSI C'ACGA_G
Cuts at: 3851 4863 3851
Size: 1012 4779
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5791
BstBI TT'CG_AA
Cuts at: 4595 4595
Size: 5791
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5791
BstXI CCAn_nnnn'nTGG
Cuts at: 2647 2647
Size: 5791
BstYI r'GATC_y
Cuts at: 1972 3035 3121 3488 3873 3890 5342 5428 5440
Size: 1063 86 367 385 17 1452 86 12
Cuts at: 5440 5786 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1063 385 367 346 86 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2359 2679 513
Size: 552 1294 320 3625
Fragments arranged by size:
3625 1294 552 320
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5052
Fragments arranged by size:
5052 683 56
BtsI GCAGTG_nn'
Cuts at: 3307 4101 4121 3307
Size: 794 20 4977
Fragments arranged by size:
4977 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5791
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5756
DraI TTT'AAA
Cuts at: 3095 3095
Size: 5791
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2447 4798 809
Size: 507 1131 2351 1802
Fragments arranged by size:
2351 1802 1131 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3238 4127 5756
Size: 54 1425 50 275 18 1408 889 1629
Cuts at: 5756 8
Size: 43
Fragments arranged by size:
1629 1425 1408 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3238 1537
Size: 275 1426 4090
Fragments arranged by size:
4090 1426 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2730 3721 1353
Size: 1377 991 3423
Fragments arranged by size:
3423 1377 991
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4378 4752 4898 387
Size: 1064 138 2789 374 146 1280
Fragments arranged by size:
2789 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2653 2653
Size: 5791
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2576 2595 2669 2948 3854 4451 5238
Size: 393 944 19 74 279 906 597 787
Cuts at: 5238 5506 1239
Size: 268 1524
Fragments arranged by size:
1524 944 906 787 597 393 279 268
74 19
EcoRI G'AATT_C
Cuts at: 3100 3478 3100
Size: 378 5413
FspI TGC'GCA
Cuts at: 4297 4297
Size: 5791
HaeII r_GCGC'y
Cuts at: 1678 4938 1678
Size: 3260 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2515 2547 2897 2929 2978
Size: 32 135 32 943 32 350 32 49
Cuts at: 2978 3010 3020 3052 4432 4464 4506 4538 1373
Size: 32 10 32 1380 32 42 32 2626
Fragments arranged by size:
2626 1380 943 350 135 49 42 32
32 32 32 32 32 32 32 10
HincII GTy'rAC
Cuts at: 669 1657 1938 3135 3381 669
Size: 988 281 1197 246 3079
Fragments arranged by size:
3079 1197 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2143 748
Size: 1395 4396
HpaI GTT'AAC
Cuts at: 3381 3381
Size: 5791
KpnI G_GTAC'C
Cuts at: 3116 3116
Size: 5791
MfeI C'AATT_G
Cuts at: 2214 3390 2214
Size: 1176 4615
MluI A'CGCG_T
Cuts at: 5667 5667
Size: 5791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4905 5089 1305
Size: 3600 184 2007
Fragments arranged by size:
3600 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3969
Fragments arranged by size:
3969 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2033 2172 2512 3749 4108 4267 518
Size: 648 867 139 340 1237 359 159 2042
Fragments arranged by size:
2042 1237 867 648 359 340 159 139
MspA1I CmG'CkG
Cuts at: 1606 2057 3088 3207 3884 5032 5277 1606
Size: 451 1031 119 677 1148 245 2120
Fragments arranged by size:
2120 1148 1031 677 451 245 119
NcoI C'CATG_G
Cuts at: 513 1065 2679 513
Size: 552 1614 3625
Fragments arranged by size:
3625 1614 552
NdeI CA'TA_TG
Cuts at: 387 3073 387
Size: 2686 3105
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5791
NotI GC'GGCC_GC
Cuts at: 3238 3238
Size: 5791
NsiI A_TGCA'T
Cuts at: 2131 2131
Size: 5791
NspI r_CATG'y
Cuts at: 1132 1229 3149 4694 1132
Size: 97 1920 1545 2229
Fragments arranged by size:
2229 1920 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5791
PciI A'CATG_T
Cuts at: 4690 4690
Size: 5791
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5791
PmeI GTTT'AAAC
Cuts at: 3095 3095
Size: 5791
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3860 3892 5373 5405 3860
Size: 32 1481 32 4246
Fragments arranged by size:
4246 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5791
PsiI TTA'TAA
Cuts at: 3361 3361
Size: 5791
PstI C_TGCA'G
Cuts at: 830 3043 3143 830
Size: 2213 100 3478
Fragments arranged by size:
3478 2213 100
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5791
PvuII CAG'CTG
Cuts at: 2057 3088 2057
Size: 1031 4760
SacI G_AGCT'C
Cuts at: 2655 2655
Size: 5791
SalI G'TCGA_C
Cuts at: 1936 3133 1936
Size: 1197 4594
SbfI CC_TGCA'GG
Cuts at: 3143 3143
Size: 5791
ScaI AGT'ACT
Cuts at: 4039 5751 4039
Size: 1712 4079
SfcI C'TryA_G
Cuts at: 826 1045 3039 3139 4274 4955 5146 826
Size: 219 1994 100 1135 681 191 1471
Fragments arranged by size:
1994 1471 1135 681 219 191 100
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5791
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5791
SmaI CCC'GGG
Cuts at: 1872 3118 1872
Size: 1246 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2140 2841 2997 3895 4796 5058
Size: 197 926 197 701 156 898 901 262
Cuts at: 5058 820
Size: 1553
Fragments arranged by size:
1553 926 901 898 701 262 197 197
156
SnaBI TAC'GTA
Cuts at: 493 3582 493
Size: 3089 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5791
SphI G_CATG'C
Cuts at: 1132 3149 1132
Size: 2017 3774
SspI AAT'ATT
Cuts at: 5 52 3715 5
Size: 47 3663 2081
Fragments arranged by size:
3663 2081 47
StuI AGG'CCT
Cuts at: 1517 2600 1517
Size: 1083 4708
StyI C'CwwG_G
Cuts at: 513 1065 2679 3230 513
Size: 552 1614 551 3074
Fragments arranged by size:
3074 1614 552 551
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2795 3818 3835 3988 4173 4618 325
Size: 1255 1215 1023 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1215 1023 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4037 5749 96
Size: 274 80 33 51 1443 2060 1712 138
Fragments arranged by size:
2060 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5751 5783 5751
Size: 32 5759
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5791
XbaI T'CTAG_A
Cuts at: 3127 3127
Size: 5791
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5791
XmaI C'CCGG_G
Cuts at: 1870 3116 1870
Size: 1246 4545
XmnI GAAnn'nnTTC
Cuts at: 3920 3920
Size: 5791
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2182 3599 276
Size: 53 83 186 936 648 1417 2468
Fragments arranged by size:
2468 1417 936 648 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI