Restriction map for
pFN21AB4702
(Circular) (Six-base) MAPSORT of: pFN21AB4702.seq Check: 1799 from: 1 to: 6427
pFN21AB4702 6427 bp
With 182 enzymes: SgfI *
October 20, 2009 10:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4237 278
Size: 53 83 186 936 2701 2468
Fragments arranged by size:
2701 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2043 3770 1937
Size: 106 1727 4594
Fragments arranged by size:
4594 1727 106
Acc65I G'GTAC_C
Cuts at: 3748 3748
Size: 6427
AclI AA'CG_TT
Cuts at: 1563 4554 4927 1563
Size: 2991 373 3063
Fragments arranged by size:
3063 2991 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4490 5874 6142 1632
Size: 2858 1384 268 1917
Fragments arranged by size:
2858 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6230
AflIII A'CryG_T
Cuts at: 1203 2830 5326 6303 1203
Size: 1627 2496 977 1327
Fragments arranged by size:
2496 1627 1327 977
AgeI A'CCGG_T
Cuts at: 5258 5258
Size: 6427
AhdI GACnn_n'nnGTC
Cuts at: 5156 5156
Size: 6427
AleI CACnn'nnGTG
Cuts at: 1166 2605 1166
Size: 1439 4988
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2233 1184
Size: 1049 5378
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2121 2153 2121
Size: 32 6395
AlwNI CAG_nnn'CTG
Cuts at: 2843 4170 5742 2843
Size: 1327 1572 3528
Fragments arranged by size:
3528 1572 1327
ApaLI G'TGCA_C
Cuts at: 4484 5640 4484
Size: 1156 5271
ApoI r'AATT_y
Cuts at: 1493 2278 3216 3287 3350 3736 3962 4114 6223
Size: 785 938 71 63 386 226 152 2109
Cuts at: 6223 1493
Size: 1697
Fragments arranged by size:
2109 1697 938 785 386 226 152 71
63
AseI AT'TA_AT
Cuts at: 160 1032 4981 160
Size: 872 3949 1606
Fragments arranged by size:
3949 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6427
AvaI C'yCGr_G
Cuts at: 1870 1943 3752 1870
Size: 73 1809 4545
Fragments arranged by size:
4545 1809 73
AvrII C'CTAG_G
Cuts at: 2245 2245
Size: 6427
BamHI G'GATC_C
Cuts at: 3757 4124 3757
Size: 367 6060
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3748 5203 618
Size: 325 217 627 1961 1455 1842
Fragments arranged by size:
1961 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3746 1391
Size: 2355 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5456
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4618 4652 1903
Size: 34 2681 34 3678
Fragments arranged by size:
3678 2681 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4319 5529 4319
Size: 1210 5217
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6427
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2058 5038 136
Size: 107 122 71 631 605 386 2980 1525
Fragments arranged by size:
2980 1525 631 605 386 122 107 71
BglII A'GATC_T
Cuts at: 6422 6422
Size: 6427
BlpI GC'TnA_GC
Cuts at: 3844 3844
Size: 6427
Bme1580I G_kGCm'C
Cuts at: 1790 4488 5644 1790
Size: 2698 1156 2573
Fragments arranged by size:
2698 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6427
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4158 5116 442
Size: 416 53 83 399 24 2741 958 1753
Fragments arranged by size:
2741 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6427
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2752 2752
Size: 6427
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2385 3579 3729 5087 1239
Size: 1146 1194 150 1358 2579
Fragments arranged by size:
2579 1358 1194 1146 150
Bpu10I CC'TnA_GC
Cuts at: 2966 2966
Size: 6427
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2442 3405 4552 5417 5715 2442
Size: 963 1147 865 298 3154
Fragments arranged by size:
3154 1147 963 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5090 882
Size: 617 3591 2219
Fragments arranged by size:
3591 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4218 493
Size: 711 3014 2702
Fragments arranged by size:
3014 2702 711
BsaBI GATnn'nnATC
Cuts at: 6421 6421
Size: 6427
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4234 4616 5251 275
Size: 53 83 186 936 2701 382 635 1451
Fragments arranged by size:
2701 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4860 5258 5532 5679 6090 4860
Size: 398 274 147 411 5197
Fragments arranged by size:
5197 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2025 2055 2121 2151 3582
Size: 30 165 30 622 30 66 30 1431
Cuts at: 3582 3612 1178
Size: 30 3993
Fragments arranged by size:
3993 1431 622 165 66 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2799 1186
Size: 1613 4814
BseYI C'CCAG_C
Cuts at: 5630 6126 5630
Size: 496 5931
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2789 1163
Size: 573 1053 4801
Fragments arranged by size:
4801 1053 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3877 4638 5666 1540
Size: 275 186 1876 761 1028 2301
Fragments arranged by size:
2301 1876 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2447 2540 3565 4488 4573 5644 2447
Size: 93 1025 923 85 1071 3230
Fragments arranged by size:
3230 1071 1025 923 93 85
BsmI GAATG_Cn'
Cuts at: 2650 2857 3938 4031 2650
Size: 207 1081 93 5046
Fragments arranged by size:
5046 1081 207 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2447 2540 3565 3746 4488 4573 5644
Size: 399 657 93 1025 181 742 85 1071
Cuts at: 5644 1391
Size: 2174
Fragments arranged by size:
2174 1071 1025 742 657 399 181 93
85
BspEI T'CCGG_A
Cuts at: 6090 6090
Size: 6427
BspHI T'CATG_A
Cuts at: 2173 2521 4316 6046 2173
Size: 348 1795 1730 2554
Fragments arranged by size:
2554 1795 1730 348
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3782 844
Size: 2938 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2036 4314 1394
Size: 429 213 2278 3507
Fragments arranged by size:
3507 2278 429 213
BsrDI GCAATG_nn'
Cuts at: 65 1248 3101 4922 5096 65
Size: 1183 1853 1821 174 1396
Fragments arranged by size:
1853 1821 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2052 5071 5258 1700
Size: 110 242 3019 187 2869
Fragments arranged by size:
3019 2869 242 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6427
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6427
BssSI C'ACGA_G
Cuts at: 4487 5499 4487
Size: 1012 5415
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2551 2852 794
Size: 1757 301 4369
Fragments arranged by size:
4369 1757 301
BstBI TT'CG_AA
Cuts at: 5231 5231
Size: 6427
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6427
BstYI r'GATC_y
Cuts at: 1972 2679 3757 4124 4509 4526 5978 6064 6076
Size: 707 1078 367 385 17 1452 86 12
Cuts at: 6076 6422 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1078 707 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4936
Fragments arranged by size:
4936 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5688
Fragments arranged by size:
5688 683 56
BtsI GCAGTG_nn'
Cuts at: 2707 3943 4737 4757 2707
Size: 1236 794 20 4377
Fragments arranged by size:
4377 1236 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6427
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6392
DraI TTT'AAA
Cuts at: 3731 3731
Size: 6427
DraIII CAC_nnn'GTG
Cuts at: 2427 2551 2427
Size: 124 6303
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5434 809
Size: 507 4118 1802
Fragments arranged by size:
4118 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2050 2158 3616
Size: 54 1425 50 275 18 220 108 1458
Cuts at: 3616 3874 4763 6392 8
Size: 258 889 1629 43
Fragments arranged by size:
1629 1458 1425 889 275 258 220 108
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3874 1537
Size: 275 2062 4090
Fragments arranged by size:
4090 2062 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2200 2202 4357 1353
Size: 847 2 2155 3423
Fragments arranged by size:
3423 2155 847 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5014 5388 5534 387
Size: 1064 138 3425 374 146 1280
Fragments arranged by size:
3425 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2385 3579 3729 4490 5087 5874 6142
Size: 393 753 1194 150 761 597 787 268
Cuts at: 6142 1239
Size: 1524
Fragments arranged by size:
1524 1194 787 761 753 597 393 268
150
EcoRI G'AATT_C
Cuts at: 3287 3736 4114 3287
Size: 449 378 5600
Fragments arranged by size:
5600 449 378
EcoRV GAT'ATC
Cuts at: 2361 2361
Size: 6427
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2420 2420
Size: 6427
FspI TGC'GCA
Cuts at: 4933 4933
Size: 6427
HaeII r_GCGC'y
Cuts at: 1678 5574 1678
Size: 3896 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2358 2390 2778 2810 3671
Size: 32 135 32 786 32 388 32 861
Cuts at: 3671 3703 5068 5100 5142 5174 1373
Size: 32 1365 32 42 32 2626
Fragments arranged by size:
2626 1365 861 786 388 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3498 3771 4017 669
Size: 988 281 1560 273 246 3079
Fragments arranged by size:
3079 1560 988 281 273 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6427
HpaI GTT'AAC
Cuts at: 3498 4017 3498
Size: 519 5908
KpnI G_GTAC'C
Cuts at: 3752 3752
Size: 6427
MfeI C'AATT_G
Cuts at: 3309 4026 3309
Size: 717 5710
MluI A'CGCG_T
Cuts at: 6303 6303
Size: 6427
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2740 3132 3329 3657 5541 5725 1305
Size: 1435 392 197 328 1884 184 2007
Fragments arranged by size:
2007 1884 1435 392 328 197 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2160 10
Size: 54 1425 343 328 4277
Fragments arranged by size:
4277 1425 343 328 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2605 2946 4385 4744 4903 518
Size: 648 1439 341 1439 359 159 2042
Fragments arranged by size:
2042 1439 1439 648 359 341 159
MspA1I CmG'CkG
Cuts at: 1606 2075 3843 4520 5668 5913 1606
Size: 469 1768 677 1148 245 2120
Fragments arranged by size:
2120 1768 1148 677 469 245
NaeI GCC'GGC
Cuts at: 2054 2054
Size: 6427
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4936
Fragments arranged by size:
4936 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6427
NgoMIV G'CCGG_C
Cuts at: 2052 2052
Size: 6427
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6427
NotI GC'GGCC_GC
Cuts at: 3874 3874
Size: 6427
NspI r_CATG'y
Cuts at: 1132 1229 2558 2834 3785 5330 1132
Size: 97 1329 276 951 1545 2229
Fragments arranged by size:
2229 1545 1329 951 276 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6427
PciI A'CATG_T
Cuts at: 2830 5326 2830
Size: 2496 3931
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3311 1108
Size: 2203 4224
PmeI GTTT'AAAC
Cuts at: 3731 3731
Size: 6427
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2121 2153 4496 4528 6009 6041 2121
Size: 32 2343 32 1481 32 2507
Fragments arranged by size:
2507 2343 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6427
PsiI TTA'TAA
Cuts at: 3997 3997
Size: 6427
PstI C_TGCA'G
Cuts at: 830 2313 2344 2848 3016 3779 830
Size: 1483 31 504 168 763 3478
Fragments arranged by size:
3478 1483 763 504 168 31
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6427
PvuII CAG'CTG
Cuts at: 2075 2075
Size: 6427
SalI G'TCGA_C
Cuts at: 1936 3769 1936
Size: 1833 4594
SapI GCTCTTCn'nnn_
Cuts at: 2200 2200
Size: 6427
SbfI CC_TGCA'GG
Cuts at: 3779 3779
Size: 6427
ScaI AGT'ACT
Cuts at: 4675 6387 4675
Size: 1712 4715
SexAI A'CCwGG_T
Cuts at: 2547 2547
Size: 6427
SfcI C'TryA_G
Cuts at: 826 1045 2076 2309 2340 2844 3012 3330 3775
Size: 219 1031 233 31 504 168 318 445
Cuts at: 3775 4910 5591 5782 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1031 681 504 445 318 233
219 191 168 31
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6427
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6427
SmaI CCC'GGG
Cuts at: 1872 3754 1872
Size: 1882 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2457 3420 4531 5432 5694 820
Size: 197 926 514 963 1111 901 262 1553
Fragments arranged by size:
1553 1111 963 926 901 514 262 197
SnaBI TAC'GTA
Cuts at: 493 4218 493
Size: 3725 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6427
SphI G_CATG'C
Cuts at: 1132 2558 3785 1132
Size: 1426 1227 3774
Fragments arranged by size:
3774 1426 1227
SspI AAT'ATT
Cuts at: 5 52 4351 5
Size: 47 4299 2081
Fragments arranged by size:
4299 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6427
StyI C'CwwG_G
Cuts at: 513 1065 2004 2245 3866 513
Size: 552 939 241 1621 3074
Fragments arranged by size:
3074 1621 939 552 241
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4454 4471 4624 4809 5254 325
Size: 1255 2874 17 153 185 445 1498
Fragments arranged by size:
2874 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4673 6385 96
Size: 274 80 33 51 1443 2696 1712 138
Fragments arranged by size:
2696 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6387 6419 6387
Size: 32 6395
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6427
XbaI T'CTAG_A
Cuts at: 3763 3763
Size: 6427
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6427
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6427
XmaI C'CCGG_G
Cuts at: 1870 3752 1870
Size: 1882 4545
XmnI GAAnn'nnTTC
Cuts at: 4556 4556
Size: 6427
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4235 276
Size: 53 83 186 936 2701 2468
Fragments arranged by size:
2701 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SfiI SfoI SrfI SwaI