Restriction map for
pFN21AB4690
(Circular) (Six-base) MAPSORT of: pFN21AB4690.seq Check: 3795 from: 1 to: 7390
pFN21AB4690 7390 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5200 278
Size: 53 83 186 936 3664 2468
Fragments arranged by size:
3664 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4733 1937
Size: 2796 4594
Acc65I G'GTAC_C
Cuts at: 3996 4711 3996
Size: 715 6675
AclI AA'CG_TT
Cuts at: 1563 5517 5890 1563
Size: 3954 373 3063
Fragments arranged by size:
3954 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2084 2465 2540 2861 3144 3827 4515 4567
Size: 452 381 75 321 283 683 688 52
Cuts at: 4567 5453 6837 7105 1632
Size: 886 1384 268 1917
Fragments arranged by size:
1917 1384 886 688 683 452 381 321
283 268 75 52
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7193
AflIII A'CryG_T
Cuts at: 1203 4059 4425 6289 7266 1203
Size: 2856 366 1864 977 1327
Fragments arranged by size:
2856 1864 1327 977 366
AgeI A'CCGG_T
Cuts at: 6221 6221
Size: 7390
AhdI GACnn_n'nnGTC
Cuts at: 2950 6119 2950
Size: 3169 4221
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7390
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2607 3551 1184
Size: 1423 944 5023
Fragments arranged by size:
5023 1423 944
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2715 2747 2715
Size: 32 7358
AlwNI CAG_nnn'CTG
Cuts at: 2936 3098 3715 4268 4385 5133 6705 2936
Size: 162 617 553 117 748 1572 3621
Fragments arranged by size:
3621 1572 748 617 553 162 117
ApaI G_GGCC'C
Cuts at: 2391 2690 2699 2804 4454 2391
Size: 299 9 105 1650 5327
Fragments arranged by size:
5327 1650 299 105 9
ApaLI G'TGCA_C
Cuts at: 5447 6603 5447
Size: 1156 6234
ApoI r'AATT_y
Cuts at: 1493 2271 2380 2866 3135 4082 4699 4925 5077
Size: 778 109 486 269 947 617 226 152
Cuts at: 5077 7186 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 947 778 617 486 269 226
152 109
AseI AT'TA_AT
Cuts at: 160 1032 5944 160
Size: 872 4912 1606
Fragments arranged by size:
4912 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7390
AvaI C'yCGr_G
Cuts at: 1870 1943 2021 2667 4715 1870
Size: 73 78 646 2048 4545
Fragments arranged by size:
4545 2048 646 78 73
BamHI G'GATC_C
Cuts at: 4510 4720 5087 4510
Size: 210 367 6813
Fragments arranged by size:
6813 367 210
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2078 2603 3996 4489 4711
Size: 325 217 627 291 525 1393 493 222
Cuts at: 4711 6166 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1393 627 525 493 325 291
222 217
BanII G_rGCy'C
Cuts at: 1391 2128 2391 2535 2690 2699 2728 2804 3044
Size: 737 263 144 155 9 29 76 240
Cuts at: 3044 3147 4454 4709 1391
Size: 103 1307 255 4072
Fragments arranged by size:
4072 1307 737 263 255 240 155 144
103 76 29 9
BbeI G_GCGC'C
Cuts at: 2082 4493 2082
Size: 2411 4979
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3650 4197 928
Size: 971 1751 547 4121
Fragments arranged by size:
4121 1751 971 547
BbvCI CC'TCA_GC
Cuts at: 4301 4347 4301
Size: 46 7344
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5581 5615 1903
Size: 34 3644 34 3678
Fragments arranged by size:
3678 3644 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3948 5282 6492 3948
Size: 1334 1210 4846
Fragments arranged by size:
4846 1334 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7390
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2076 2111 2187
Size: 107 122 71 631 605 404 35 76
Cuts at: 2187 2694 4449 4659 6001 136
Size: 507 1755 210 1342 1525
Fragments arranged by size:
1755 1525 1342 631 605 507 404 210
122 107 76 71 35
BglII A'GATC_T
Cuts at: 3826 7385 3826
Size: 3559 3831
BlpI GC'TnA_GC
Cuts at: 2454 4599 4638 4807 2454
Size: 2145 39 169 5037
Fragments arranged by size:
5037 2145 169 39
Bme1580I G_kGCm'C
Cuts at: 1790 2391 2606 2690 2699 2804 4454 5451 6607
Size: 601 215 84 9 105 1650 997 1156
Cuts at: 6607 1790
Size: 2573
Fragments arranged by size:
2573 1650 1156 997 601 215 105 84
9
BmgBI CAC'GTC
Cuts at: 1318 4060 1318
Size: 2742 4648
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2603 2684 4047
Size: 416 53 83 399 24 1186 81 1363
Cuts at: 4047 5121 6079 442
Size: 1074 958 1753
Fragments arranged by size:
1753 1363 1186 1074 958 416 399 83
81 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7390
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3079 3079
Size: 7390
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2049 2588 2664 2784 3263 4008 4269 6050
Size: 810 539 76 120 479 745 261 1781
Cuts at: 6050 1239
Size: 2579
Fragments arranged by size:
2579 1781 810 745 539 479 261 120
76
Bpu10I CC'TnA_GC
Cuts at: 3459 3842 4301 4347 4461 3459
Size: 383 459 46 114 6388
Fragments arranged by size:
6388 459 383 114 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3383 3461 4548 5515 6380 6678 3383
Size: 78 1087 967 865 298 4095
Fragments arranged by size:
4095 1087 967 865 298 78
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4274 6053 882
Size: 617 2775 1779 2219
Fragments arranged by size:
2775 2219 1779 617
BsaAI yAC'GTr
Cuts at: 493 1204 5181 493
Size: 711 3977 2702
Fragments arranged by size:
3977 2702 711
BsaBI GATnn'nnATC
Cuts at: 7384 7384
Size: 7390
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2079 4490 5197 5579
Size: 53 83 186 936 546 2411 707 382
Cuts at: 5579 6214 275
Size: 635 1451
Fragments arranged by size:
2411 1451 936 707 635 546 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2976 5823 6221 6495 6642 7053 2976
Size: 2847 398 274 147 411 3313
Fragments arranged by size:
3313 2847 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2715 2745 4670 4700 1178
Size: 30 165 30 1312 30 1925 30 3868
Fragments arranged by size:
3868 1925 1312 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2162 2307 2388 2447 2450 2501 2646 2954
Size: 976 145 81 59 3 51 145 308
Cuts at: 2954 3296 4334 4553 1186
Size: 342 1038 219 4023
Fragments arranged by size:
4023 1038 976 342 308 219 145 145
81 59 51 3
BseYI C'CCAG_C
Cuts at: 3012 3091 4011 6593 7089 3012
Size: 79 920 2582 496 3313
Fragments arranged by size:
3313 2582 920 496 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6817
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2207 4840 5601 6629 1540
Size: 275 186 206 2633 761 1028 2301
Fragments arranged by size:
2633 2301 1028 761 275 206 186
BsiHKAI G_wGCw'C
Cuts at: 2128 2535 3044 3288 4605 5451 5536 6607 2128
Size: 407 509 244 1317 846 85 1071 2911
Fragments arranged by size:
2911 1317 1071 846 509 407 244 85
BsmI GAATG_Cn'
Cuts at: 4901 4994 4901
Size: 93 7297
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2128 2391 2535 2606 2690 2699 2728
Size: 399 338 263 144 71 84 9 29
Cuts at: 2728 2804 3044 3147 3288 4454 4605 4709 5451
Size: 76 240 103 141 1166 151 104 742
Cuts at: 5451 5536 6607 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1166 1071 742 399 338 263 240
151 144 141 104 103 85 84 76
71 29 9
BspEI T'CCGG_A
Cuts at: 7053 7053
Size: 7390
BspHI T'CATG_A
Cuts at: 5279 7009 5279
Size: 1730 5660
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3543 4745 844
Size: 2699 1202 3489
Fragments arranged by size:
3489 2699 1202
BsrBI CCG'CTC
Cuts at: 1394 1823 2161 2240 2440 4048 5277 1394
Size: 429 338 79 200 1608 1229 3507
Fragments arranged by size:
3507 1608 1229 429 338 200 79
BsrDI GCAATG_nn'
Cuts at: 65 1248 2468 5885 6059 65
Size: 1183 1220 3417 174 1396
Fragments arranged by size:
3417 1396 1220 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2206 6034 6221 1700
Size: 110 396 3828 187 2869
Fragments arranged by size:
3828 2869 396 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7390
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7390
BssSI C'ACGA_G
Cuts at: 2742 5450 6462 2742
Size: 2708 1012 3670
Fragments arranged by size:
3670 2708 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7390
BstBI TT'CG_AA
Cuts at: 6194 6194
Size: 7390
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7390
BstXI CCAn_nnnn'nTGG
Cuts at: 3513 4007 3513
Size: 494 6896
BstYI r'GATC_y
Cuts at: 1972 2808 2829 3168 3826 4510 4720 5087 5472
Size: 836 21 339 658 684 210 367 385
Cuts at: 5472 5489 6941 7027 7039 7385 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 836 684 658 385 367 346
339 210 86 21 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2718 3076 3763 3896 4431 4654
Size: 552 939 714 358 687 133 535 223
Cuts at: 4654 513
Size: 3249
Fragments arranged by size:
3249 939 714 687 552 535 358 223
133
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4340 487
Size: 683 56 3114 3537
Fragments arranged by size:
3537 3114 683 56
BtsI GCAGTG_nn'
Cuts at: 3316 4906 5700 5720 3316
Size: 1590 794 20 4986
Fragments arranged by size:
4986 1590 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7390
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2274 2309 2364 2399 529
Size: 35 1710 35 55 35 5520
Fragments arranged by size:
5520 1710 55 35 35 35
DraI TTT'AAA
Cuts at: 4694 4694
Size: 7390
DraIII CAC_nnn'GTG
Cuts at: 3442 3442
Size: 7390
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3652 6397 809
Size: 507 2336 2745 1802
Fragments arranged by size:
2745 2336 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2204 4518 4837
Size: 54 1425 50 275 18 374 2314 319
Cuts at: 4837 5726 7355 8
Size: 889 1629 43
Fragments arranged by size:
2314 1629 1425 889 374 319 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2204 4837 1537
Size: 275 392 2633 4090
Fragments arranged by size:
4090 2633 392 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2853 2877 3273 4101 4161 4167 4358 4367
Size: 1500 24 396 828 60 6 191 9
Cuts at: 4367 4491 5320 1353
Size: 124 829 3423
Fragments arranged by size:
3423 1500 829 828 396 191 124 60
24 9 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2310 5977 6351 6497 387
Size: 1064 138 721 3667 374 146 1280
Fragments arranged by size:
3667 1280 1064 721 374 146 138
EcoICRI GAG'CTC
Cuts at: 2126 2533 3042 2126
Size: 407 509 6474
Fragments arranged by size:
6474 509 407
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2049 2084 2465 2540 2588 2664 2784
Size: 393 417 35 381 75 48 76 120
Cuts at: 2784 2861 3144 3263 3827 4008 4269 4515 4567
Size: 77 283 119 564 181 261 246 52
Cuts at: 4567 5453 6050 6837 7105 1239
Size: 886 597 787 268 1524
Fragments arranged by size:
1524 886 787 597 564 417 393 381
283 268 261 246 181 120 119 77
76 75 52 48 35
EcoNI CCTnn'n_nnAGG
Cuts at: 2340 2340
Size: 7390
EcoO109I rG'GnC_Cy
Cuts at: 2387 2388 2663 2696 2801 3454 3619 3769 3886
Size: 1 275 33 105 653 165 150 117
Cuts at: 3886 4450 2387
Size: 564 5327
Fragments arranged by size:
5327 653 564 275 165 150 117 105
33 1
EcoRI G'AATT_C
Cuts at: 2271 2866 4699 5077 2271
Size: 595 1833 378 4584
Fragments arranged by size:
4584 1833 595 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3187 3583 4597 3187
Size: 396 1014 5980
Fragments arranged by size:
5980 1014 396
FspI TGC'GCA
Cuts at: 2651 5896 2651
Size: 3245 4145
FspAI rTGC'GCAy
Cuts at: 2651 2651
Size: 7390
HaeII r_GCGC'y
Cuts at: 1678 2082 4487 4493 6537 1678
Size: 404 2405 6 2044 2531
Fragments arranged by size:
2531 2405 2044 404 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2002 2034 2110 2115 2142
Size: 32 135 32 430 32 76 5 27
Cuts at: 2142 2147 2777 2809 2854 2886 2891 2923 2936
Size: 5 630 32 45 32 5 32 13
Cuts at: 2936 2968 4139 4171 6031 6063 6105 6137 1373
Size: 32 1171 32 1860 32 42 32 2626
Fragments arranged by size:
2626 1860 1171 630 430 135 76 45
42 32 32 32 32 32 32 32
32 32 32 27 13 5 5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 4734 4980 669
Size: 988 281 2796 246 3079
Fragments arranged by size:
3079 2796 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7390
HpaI GTT'AAC
Cuts at: 4980 4980
Size: 7390
KasI G'GCGC_C
Cuts at: 2078 4489 2078
Size: 2411 4979
KpnI G_GTAC'C
Cuts at: 4000 4715 4000
Size: 715 6675
MfeI C'AATT_G
Cuts at: 4989 4989
Size: 7390
MluI A'CGCG_T
Cuts at: 7266 7266
Size: 7390
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4369 4482 6504 6688 1305
Size: 3064 113 2022 184 2007
Fragments arranged by size:
3064 2022 2007 184 113
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4520 10
Size: 54 1425 343 2688 2880
Fragments arranged by size:
2880 2688 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4005 4419 4436 5348 5707 5866 518
Size: 648 2839 414 17 912 359 159 2042
Fragments arranged by size:
2839 2042 912 648 414 359 159 17
MspA1I CmG'CkG
Cuts at: 1606 2047 2558 2839 3016 3098 3406 4806 5483
Size: 441 511 281 177 82 308 1400 677
Cuts at: 5483 6631 6876 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1400 1148 677 511 441 308 281
245 177 82
NarI GG'CG_CC
Cuts at: 2079 4490 2079
Size: 2411 4979
NcoI C'CATG_G
Cuts at: 513 1065 2004 2718 3763 3896 513
Size: 552 939 714 1045 133 4007
Fragments arranged by size:
4007 1045 939 714 552 133
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7390
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7390
NotI GC'GGCC_GC
Cuts at: 4837 4837
Size: 7390
NruI TCG'CGA
Cuts at: 2145 2145
Size: 7390
NspI r_CATG'y
Cuts at: 1132 1229 4429 4547 4748 6293 1132
Size: 97 3200 118 201 1545 2229
Fragments arranged by size:
3200 2229 1545 201 118 97
PasI CC'CwG_GG
Cuts at: 1348 2914 3956 1348
Size: 1566 1042 4782
Fragments arranged by size:
4782 1566 1042
PciI A'CATG_T
Cuts at: 4425 6289 4425
Size: 1864 5526
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2348 2821 1108
Size: 1240 473 5677
Fragments arranged by size:
5677 1240 473
PfoI T'CCnGG_A
Cuts at: 3603 4246 3603
Size: 643 6747
PmeI GTTT'AAAC
Cuts at: 4694 4694
Size: 7390
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2517 2549 2715 2747 3657 3689 5459 5491 6972
Size: 32 166 32 910 32 1770 32 1481
Cuts at: 6972 7004 2517
Size: 32 2903
Fragments arranged by size:
2903 1770 1481 910 166 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2663 3454 3769 3886 2663
Size: 791 315 117 6167
Fragments arranged by size:
6167 791 315 117
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7390
PsiI TTA'TAA
Cuts at: 4960 4960
Size: 7390
PspOMI G'GGCC_C
Cuts at: 2387 2686 2695 2800 4450 2387
Size: 299 9 105 1650 5327
Fragments arranged by size:
5327 1650 299 105 9
PspXI vC'TCGA_Gb
Cuts at: 2667 2667
Size: 7390
PstI C_TGCA'G
Cuts at: 830 2169 2313 2838 3256 3540 3627 4228 4742
Size: 1339 144 525 418 284 87 601 514
Cuts at: 4742 830
Size: 3478
Fragments arranged by size:
3478 1339 601 525 514 418 284 144
87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7390
PvuII CAG'CTG
Cuts at: 2047 2558 2839 3098 3406 2047
Size: 511 281 259 308 6031
Fragments arranged by size:
6031 511 308 281 259
SacI G_AGCT'C
Cuts at: 2128 2535 3044 2128
Size: 407 509 6474
Fragments arranged by size:
6474 509 407
SalI G'TCGA_C
Cuts at: 1936 4732 1936
Size: 2796 4594
SapI GCTCTTCn'nnn_
Cuts at: 4358 4358
Size: 7390
SbfI CC_TGCA'GG
Cuts at: 3540 3627 4742 3540
Size: 87 1115 6188
Fragments arranged by size:
6188 1115 87
ScaI AGT'ACT
Cuts at: 5638 7350 5638
Size: 1712 5678
SfcI C'TryA_G
Cuts at: 826 1045 2165 2309 2706 2834 3252 3536 3623
Size: 219 1120 144 397 128 418 284 87
Cuts at: 3623 4224 4738 5873 6554 6745 826
Size: 601 514 1135 681 191 1471
Fragments arranged by size:
1471 1135 1120 681 601 514 418 397
284 219 191 144 128 87
SfiI GGCCn_nnn'nGGCC
Cuts at: 2694 2694
Size: 7390
SfoI GGC'GCC
Cuts at: 2080 4491 2080
Size: 2411 4979
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7390
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7390
SmaI CCC'GGG
Cuts at: 1872 4717 1872
Size: 2845 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2667 3398 3476 4563 5494 6395
Size: 197 926 724 731 78 1087 931 901
Cuts at: 6395 6657 820
Size: 262 1553
Fragments arranged by size:
1553 1087 931 926 901 731 724 262
197 78
SnaBI TAC'GTA
Cuts at: 493 5181 493
Size: 4688 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7390
SphI G_CATG'C
Cuts at: 1132 4748 1132
Size: 3616 3774
SspI AAT'ATT
Cuts at: 5 52 5314 5
Size: 47 5262 2081
Fragments arranged by size:
5262 2081 47
StuI AGG'CCT
Cuts at: 1517 2346 1517
Size: 829 6561
StyI C'CwwG_G
Cuts at: 513 1065 2004 2347 2353 2718 3763 3852 3896
Size: 552 939 343 6 365 1045 89 44
Cuts at: 3896 4124 4829 513
Size: 228 705 3074
Fragments arranged by size:
3074 1045 939 705 552 365 343 228
89 44 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2388 2399 5417 5434 5587 5772 6217
Size: 1255 808 11 3018 17 153 185 445
Cuts at: 6217 325
Size: 1498
Fragments arranged by size:
3018 1498 1255 808 445 185 153 17
11
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3774 5636 7348
Size: 274 80 33 51 1443 1797 1862 1712
Cuts at: 7348 96
Size: 138
Fragments arranged by size:
1862 1797 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7350 7382 7350
Size: 32 7358
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7390
XbaI T'CTAG_A
Cuts at: 4726 4726
Size: 7390
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4652 4652
Size: 7390
XhoI C'TCGA_G
Cuts at: 1943 2667 1943
Size: 724 6666
XmaI C'CCGG_G
Cuts at: 1870 4715 1870
Size: 2845 4545
XmnI GAAnn'nnTTC
Cuts at: 3380 3791 5519 3380
Size: 411 1728 5251
Fragments arranged by size:
5251 1728 411
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5198 276
Size: 53 83 186 936 3664 2468
Fragments arranged by size:
3664 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NruI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I EcoRV FseI NaeI NgoMIV NsiI PacI
PmlI PsrI RsrII SacII SanDI SexAI SrfI SwaI