Restriction map for
pFN21AB4688
(Circular) (Six-base) MAPSORT of: pFN21AB4688.seq Check: 8620 from: 1 to: 6436
pFN21AB4688 6436 bp
With 182 enzymes: SgfI *
October 20, 2009 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2959 2959
Size: 6436
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4246 278
Size: 53 83 186 936 2710 2468
Fragments arranged by size:
2710 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2149 3779 1937
Size: 212 1630 4594
Fragments arranged by size:
4594 1630 212
Acc65I G'GTAC_C
Cuts at: 3757 3757
Size: 6436
AclI AA'CG_TT
Cuts at: 1563 4563 4936 1563
Size: 3000 373 3063
Fragments arranged by size:
3063 3000 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2771 3420 4499 5883 6151 1632
Size: 1139 649 1079 1384 268 1917
Fragments arranged by size:
1917 1384 1139 1079 649 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6239
AflIII A'CryG_T
Cuts at: 1203 5335 6312 1203
Size: 4132 977 1327
Fragments arranged by size:
4132 1327 977
AgeI A'CCGG_T
Cuts at: 5267 5267
Size: 6436
AhdI GACnn_n'nnGTC
Cuts at: 5165 5165
Size: 6436
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6436
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3261 1184
Size: 2077 4359
AlwNI CAG_nnn'CTG
Cuts at: 3241 4179 5751 3241
Size: 938 1572 3926
Fragments arranged by size:
3926 1572 938
ApaLI G'TGCA_C
Cuts at: 4493 5649 4493
Size: 1156 5280
ApoI r'AATT_y
Cuts at: 1493 2605 3252 3551 3745 3971 4123 6232 1493
Size: 1112 647 299 194 226 152 2109 1697
Fragments arranged by size:
2109 1697 1112 647 299 226 194 152
AseI AT'TA_AT
Cuts at: 160 1032 4990 160
Size: 872 3958 1606
Fragments arranged by size:
3958 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6436
AvaI C'yCGr_G
Cuts at: 1870 1943 2344 2619 3269 3372 3761 1870
Size: 73 401 275 650 103 389 4545
Fragments arranged by size:
4545 650 401 389 275 103 73
BamHI G'GATC_C
Cuts at: 2478 3766 4133 2478
Size: 1288 367 4781
Fragments arranged by size:
4781 1288 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3005 3265 3392 3757 5212
Size: 325 217 627 1218 260 127 365 1455
Cuts at: 5212 618
Size: 1842
Fragments arranged by size:
1842 1455 1218 627 365 325 260 217
127
BanII G_rGCy'C
Cuts at: 1391 2496 3755 1391
Size: 1105 1259 4072
Fragments arranged by size:
4072 1259 1105
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3122 928
Size: 971 1223 4242
Fragments arranged by size:
4242 1223 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2444 2478 4627 4661 1903
Size: 34 507 34 2149 34 3678
Fragments arranged by size:
3678 2149 507 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4328 5538 4328
Size: 1210 5226
BclI T'GATC_A
Cuts at: 1548 3591 1548
Size: 2043 4393
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2506 5047 136
Size: 107 122 71 631 605 834 2541 1525
Fragments arranged by size:
2541 1525 834 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6431 6431
Size: 6436
BlpI GC'TnA_GC
Cuts at: 3853 3853
Size: 6436
Bme1580I G_kGCm'C
Cuts at: 1790 3008 3270 3395 4497 5653 1790
Size: 1218 262 125 1102 1156 2573
Fragments arranged by size:
2573 1218 1156 1102 262 125
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6436
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4167 5125 442
Size: 416 53 83 399 24 2750 958 1753
Fragments arranged by size:
2750 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6436
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2531 3071 5096 1239
Size: 1292 540 2025 2579
Fragments arranged by size:
2579 2025 1292 540
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1995 2762 3086 4561 5426 5724 1995
Size: 767 324 1475 865 298 2707
Fragments arranged by size:
2707 1475 865 767 324 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3404 3460 5099 882
Size: 617 1905 56 1639 2219
Fragments arranged by size:
2219 1905 1639 617 56
BsaAI yAC'GTr
Cuts at: 493 1204 3445 4227 493
Size: 711 2241 782 2702
Fragments arranged by size:
2702 2241 782 711
BsaBI GATnn'nnATC
Cuts at: 6430 6430
Size: 6436
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4243 4625 5260 275
Size: 53 83 186 936 2710 382 635 1451
Fragments arranged by size:
2710 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4869 5267 5541 5688 6099 4869
Size: 398 274 147 411 5206
Fragments arranged by size:
5206 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2900 2930 1178
Size: 30 165 30 1497 30 4684
Fragments arranged by size:
4684 1497 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2343 2759 2915 3125 3565 1186
Size: 1157 416 156 210 440 4057
Fragments arranged by size:
4057 1157 440 416 210 156
BseYI C'CCAG_C
Cuts at: 5639 6135 5639
Size: 496 5940
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2220 1163
Size: 573 484 5379
Fragments arranged by size:
5379 573 484
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3886 4647 5675 1540
Size: 275 186 1885 761 1028 2301
Fragments arranged by size:
2301 1885 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4497 4582 5653 4497
Size: 85 1071 5280
Fragments arranged by size:
5280 1071 85
BsmI GAATG_Cn'
Cuts at: 3947 4040 3947
Size: 93 6343
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2496 3008 3270 3395 3755 4497 4582
Size: 399 706 512 262 125 360 742 85
Cuts at: 4582 5653 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 706 512 399 360 262
125 85
BspEI T'CCGG_A
Cuts at: 6099 6099
Size: 6436
BspHI T'CATG_A
Cuts at: 4325 6055 4325
Size: 1730 4706
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2135 2959 3791 844
Size: 1291 824 832 3489
Fragments arranged by size:
3489 1291 832 824
BsrBI CCG'CTC
Cuts at: 1394 1823 4323 1394
Size: 429 2500 3507
Fragments arranged by size:
3507 2500 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3037 4931 5105 65
Size: 1183 1789 1894 174 1396
Fragments arranged by size:
1894 1789 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5080 5267 1700
Size: 110 3270 187 2869
Fragments arranged by size:
3270 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6436
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6436
BssSI C'ACGA_G
Cuts at: 3463 4496 5508 3463
Size: 1033 1012 4391
Fragments arranged by size:
4391 1033 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6436
BstBI TT'CG_AA
Cuts at: 5240 5240
Size: 6436
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6436
BstYI r'GATC_y
Cuts at: 1972 2478 2883 3054 3766 4133 4518 4535 5987
Size: 506 405 171 712 367 385 17 1452
Cuts at: 5987 6073 6085 6431 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 712 506 405 385 367 346
171 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3261 3351 3451 3598 513
Size: 552 2196 90 100 147 3351
Fragments arranged by size:
3351 2196 552 147 100 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5697
Fragments arranged by size:
5697 683 56
BtsI GCAGTG_nn'
Cuts at: 2988 3952 4746 4766 2988
Size: 964 794 20 4658
Fragments arranged by size:
4658 964 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6436
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6401
DraI TTT'AAA
Cuts at: 3420 3740 3420
Size: 320 6116
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5443 809
Size: 507 4127 1802
Fragments arranged by size:
4127 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2848 3021 3883
Size: 54 1425 50 275 18 1018 173 862
Cuts at: 3883 4772 6401 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1018 889 862 275 173 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3883 1537
Size: 275 2071 4090
Fragments arranged by size:
4090 2071 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2088 2518 3439 4366 1353
Size: 735 430 921 927 3423
Fragments arranged by size:
3423 927 921 735 430
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5023 5397 5543 387
Size: 1064 138 3434 374 146 1280
Fragments arranged by size:
3434 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2531 2771 3071 3420 4499 5096 5883
Size: 393 899 240 300 349 1079 597 787
Cuts at: 5883 6151 1239
Size: 268 1524
Fragments arranged by size:
1524 1079 899 787 597 393 349 300
268 240
EcoRI G'AATT_C
Cuts at: 3252 3745 4123 3252
Size: 493 378 5565
Fragments arranged by size:
5565 493 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2411 2411
Size: 6436
FspI TGC'GCA
Cuts at: 4942 4942
Size: 6436
HaeII r_GCGC'y
Cuts at: 1678 5583 1678
Size: 3905 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2276 2308 3208 3240 3605
Size: 32 135 32 704 32 900 32 365
Cuts at: 3605 3637 5077 5109 5151 5183 1373
Size: 32 1440 32 42 32 2626
Fragments arranged by size:
2626 1440 900 704 365 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2284 3780 4026 669
Size: 988 281 346 1496 246 3079
Fragments arranged by size:
3079 1496 988 346 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6436
HpaI GTT'AAC
Cuts at: 4026 4026
Size: 6436
KpnI G_GTAC'C
Cuts at: 3761 3761
Size: 6436
MfeI C'AATT_G
Cuts at: 3568 4035 3568
Size: 467 5969
MluI A'CGCG_T
Cuts at: 6312 6312
Size: 6436
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2959 3651 5550 5734 1305
Size: 1654 692 1899 184 2007
Fragments arranged by size:
2007 1899 1654 692 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2850 3023 10
Size: 54 1425 343 1018 173 3423
Fragments arranged by size:
3423 1425 1018 343 173 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4394 4753 4912 518
Size: 648 3228 359 159 2042
Fragments arranged by size:
3228 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2204 3241 3852 4529 5677 5922 1606
Size: 598 1037 611 677 1148 245 2120
Fragments arranged by size:
2120 1148 1037 677 611 598 245
NcoI C'CATG_G
Cuts at: 513 1065 3261 3451 3598 513
Size: 552 2196 190 147 3351
Fragments arranged by size:
3351 2196 552 190 147
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6436
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6436
NotI GC'GGCC_GC
Cuts at: 3883 3883
Size: 6436
NspI r_CATG'y
Cuts at: 1132 1229 3049 3794 5339 1132
Size: 97 1820 745 1545 2229
Fragments arranged by size:
2229 1820 1545 745 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6436
PciI A'CATG_T
Cuts at: 5335 5335
Size: 6436
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2846 3140 3634 1108
Size: 1738 294 494 3910
Fragments arranged by size:
3910 1738 494 294
PmeI GTTT'AAAC
Cuts at: 3740 3740
Size: 6436
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3194 3226 4505 4537 6018 6050 3194
Size: 32 1279 32 1481 32 3580
Fragments arranged by size:
3580 1481 1279 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3715 1613
Size: 2102 4334
PsiI TTA'TAA
Cuts at: 4006 4006
Size: 6436
PstI C_TGCA'G
Cuts at: 830 2209 2487 2776 2995 3788 830
Size: 1379 278 289 219 793 3478
Fragments arranged by size:
3478 1379 793 289 278 219
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6436
PvuII CAG'CTG
Cuts at: 2204 3241 2204
Size: 1037 5399
SalI G'TCGA_C
Cuts at: 1936 3778 1936
Size: 1842 4594
SbfI CC_TGCA'GG
Cuts at: 3788 3788
Size: 6436
ScaI AGT'ACT
Cuts at: 4684 6396 4684
Size: 1712 4724
SfcI C'TryA_G
Cuts at: 826 1045 2205 2226 2483 2772 2991 3285 3784
Size: 219 1160 21 257 289 219 294 499
Cuts at: 3784 4919 5600 5791 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1160 1135 681 499 294 289 257
219 219 191 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6436
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6436
SmaI CCC'GGG
Cuts at: 1872 2621 3763 1872
Size: 749 1142 4545
Fragments arranged by size:
4545 1142 749
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2010 2741 3101 4540 5441 5703
Size: 197 926 67 731 360 1439 901 262
Cuts at: 5703 820
Size: 1553
Fragments arranged by size:
1553 1439 926 901 731 360 262 197
67
SnaBI TAC'GTA
Cuts at: 493 4227 493
Size: 3734 2702
SpeI A'CTAG_T
Cuts at: 152 3191 152
Size: 3039 3397
SphI G_CATG'C
Cuts at: 1132 3794 1132
Size: 2662 3774
SspI AAT'ATT
Cuts at: 5 52 4360 5
Size: 47 4308 2081
Fragments arranged by size:
4308 2081 47
StuI AGG'CCT
Cuts at: 1517 2940 1517
Size: 1423 5013
StyI C'CwwG_G
Cuts at: 513 1065 2658 3058 3261 3345 3451 3598 3875
Size: 552 1593 400 203 84 106 147 277
Cuts at: 3875 513
Size: 3074
Fragments arranged by size:
3074 1593 552 400 277 203 147 106
84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3136 4463 4480 4633 4818 5263 325
Size: 1255 1556 1327 17 153 185 445 1498
Fragments arranged by size:
1556 1498 1327 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2916 3683 4682
Size: 274 80 33 51 1443 939 767 999
Cuts at: 4682 6394 96
Size: 1712 138
Fragments arranged by size:
1712 1443 999 939 767 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6396 6428 6396
Size: 32 6404
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6436
XbaI T'CTAG_A
Cuts at: 3772 3772
Size: 6436
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3016 3449 3016
Size: 433 6003
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6436
XmaI C'CCGG_G
Cuts at: 1870 2619 3761 1870
Size: 749 1142 4545
Fragments arranged by size:
4545 1142 749
XmnI GAAnn'nnTTC
Cuts at: 4565 4565
Size: 6436
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4244 276
Size: 53 83 186 936 2710 2468
Fragments arranged by size:
2710 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI