Restriction map for
pFN21AB4679
(Circular) (Six-base) MAPSORT of: pFN21AB4679.seq Check: 3036 from: 1 to: 5308
pFN21AB4679 5308 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2426 2426
Size: 5308
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3118 278
Size: 53 83 186 936 1582 2468
Fragments arranged by size:
2468 1582 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2651 1937
Size: 714 4594
Acc65I G'GTAC_C
Cuts at: 2247 2629 2247
Size: 382 4926
AclI AA'CG_TT
Cuts at: 1563 3435 3808 1563
Size: 1872 373 3063
Fragments arranged by size:
3063 1872 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3371 4755 5023 1632
Size: 1739 1384 268 1917
Fragments arranged by size:
1917 1739 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5111
AflIII A'CryG_T
Cuts at: 1203 2106 2567 4207 5184 1203
Size: 903 461 1640 977 1327
Fragments arranged by size:
1640 1327 977 903 461
AgeI A'CCGG_T
Cuts at: 4139 4139
Size: 5308
AhdI GACnn_n'nnGTC
Cuts at: 4037 4037
Size: 5308
AleI CACnn'nnGTG
Cuts at: 1166 2427 1166
Size: 1261 4047
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5308
AlwNI CAG_nnn'CTG
Cuts at: 3051 4623 3051
Size: 1572 3736
ApaLI G'TGCA_C
Cuts at: 3365 4521 3365
Size: 1156 4152
ApoI r'AATT_y
Cuts at: 1493 2617 2843 2995 5104 1493
Size: 1124 226 152 2109 1697
Fragments arranged by size:
2109 1697 1124 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3862 160
Size: 872 2830 1606
Fragments arranged by size:
2830 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5308
AvaI C'yCGr_G
Cuts at: 1870 1943 2328 2633 1870
Size: 73 385 305 4545
Fragments arranged by size:
4545 385 305 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2588 2621 2588
Size: 33 5275
BamHI G'GATC_C
Cuts at: 2638 3005 2638
Size: 367 4941
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2247 2629 4084 618
Size: 325 217 627 460 382 1455 1842
Fragments arranged by size:
1842 1455 627 460 382 325 217
BanII G_rGCy'C
Cuts at: 1391 2326 2627 1391
Size: 935 301 4072
Fragments arranged by size:
4072 935 301
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2214 928
Size: 971 315 4022
Fragments arranged by size:
4022 971 315
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2461 2495 3499 3533 1903
Size: 34 524 34 1004 34 3678
Fragments arranged by size:
3678 1004 524 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2589 3200 4410 2589
Size: 611 1210 3487
Fragments arranged by size:
3487 1210 611
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5308
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2298 3919 136
Size: 107 122 71 631 605 626 1621 1525
Fragments arranged by size:
1621 1525 631 626 605 122 107 71
BglII A'GATC_T
Cuts at: 2546 5303 2546
Size: 2757 2551
BlpI GC'TnA_GC
Cuts at: 2725 2725
Size: 5308
Bme1580I G_kGCm'C
Cuts at: 1790 3369 4525 1790
Size: 1579 1156 2573
Fragments arranged by size:
2573 1579 1156
BmgBI CAC'GTC
Cuts at: 1318 2107 1318
Size: 789 4519
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3039 3997 442
Size: 416 53 83 399 24 1622 958 1753
Fragments arranged by size:
1753 1622 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5308
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2061 3968 1239
Size: 822 1907 2579
Fragments arranged by size:
2579 1907 822
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2129 3433 4298 4596 2129
Size: 1304 865 298 2841
Fragments arranged by size:
2841 1304 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3971 882
Size: 617 2472 2219
Fragments arranged by size:
2472 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2062 2570 3099 493
Size: 711 858 508 529 2702
Fragments arranged by size:
2702 858 711 529 508
BsaBI GATnn'nnATC
Cuts at: 5302 5302
Size: 5308
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3115 3497 4132 275
Size: 53 83 186 936 1582 382 635 1451
Fragments arranged by size:
1582 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3741 4139 4413 4560 4971 3741
Size: 398 274 147 411 4078
Fragments arranged by size:
4078 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2004 2034 1178
Size: 30 165 30 601 30 4452
Fragments arranged by size:
4452 601 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5308
BseYI C'CCAG_C
Cuts at: 4511 5007 4511
Size: 496 4812
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4735
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2281 2758 3519 4547 1540
Size: 275 186 280 477 761 1028 2301
Fragments arranged by size:
2301 1028 761 477 280 275 186
BsiHKAI G_wGCw'C
Cuts at: 2326 3369 3454 4525 2326
Size: 1043 85 1071 3109
Fragments arranged by size:
3109 1071 1043 85
BsmI GAATG_Cn'
Cuts at: 2819 2912 2819
Size: 93 5215
BsmBI CGTCTCn'nnnn_
Cuts at: 2193 2193
Size: 5308
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2326 2627 3369 3454 4525 1391
Size: 399 536 301 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 536 399 301 85
BspEI T'CCGG_A
Cuts at: 4971 4971
Size: 5308
BspHI T'CATG_A
Cuts at: 2551 3197 4927 2551
Size: 646 1730 2932
Fragments arranged by size:
2932 1730 646
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2426 2663 844
Size: 1582 237 3489
Fragments arranged by size:
3489 1582 237
BsrBI CCG'CTC
Cuts at: 1394 1823 3195 1394
Size: 429 1372 3507
Fragments arranged by size:
3507 1372 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3803 3977 65
Size: 1183 2555 174 1396
Fragments arranged by size:
2555 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3952 4139 1700
Size: 110 2142 187 2869
Fragments arranged by size:
2869 2142 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5308
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5308
BssSI C'ACGA_G
Cuts at: 3368 4380 3368
Size: 1012 4296
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5308
BstBI TT'CG_AA
Cuts at: 2521 4112 2521
Size: 1591 3717
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5308
BstYI r'GATC_y
Cuts at: 1972 2546 2638 3005 3390 3407 4859 4945 4957
Size: 574 92 367 385 17 1452 86 12
Cuts at: 4957 5303 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 574 385 367 346 92 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2155 2281 513
Size: 552 1090 126 3540
Fragments arranged by size:
3540 1090 552 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4569
Fragments arranged by size:
4569 683 56
BtsI GCAGTG_nn'
Cuts at: 2824 3618 3638 2824
Size: 794 20 4494
Fragments arranged by size:
4494 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5308
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5273
DraI TTT'AAA
Cuts at: 2415 2612 2415
Size: 197 5111
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4315 809
Size: 507 2999 1802
Fragments arranged by size:
2999 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2278 2755 3644
Size: 54 1425 50 275 18 448 477 889
Cuts at: 3644 5273 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 477 448 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2278 2755 1537
Size: 275 466 477 4090
Fragments arranged by size:
4090 477 466 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3238 1353
Size: 1885 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2334 3895 4269 4415 387
Size: 1064 138 745 1561 374 146 1280
Fragments arranged by size:
1561 1280 1064 745 374 146 138
EcoICRI GAG'CTC
Cuts at: 2324 2324
Size: 5308
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2061 3371 3968 4755 5023 1239
Size: 393 429 1310 597 787 268 1524
Fragments arranged by size:
1524 1310 787 597 429 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2191 2191
Size: 5308
EcoRI G'AATT_C
Cuts at: 2617 2995 2617
Size: 378 4930
FspI TGC'GCA
Cuts at: 3814 3814
Size: 5308
HaeII r_GCGC'y
Cuts at: 1678 2127 4455 1678
Size: 449 2328 2531
Fragments arranged by size:
2531 2328 449
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2361 2393 3949 3981 4023
Size: 32 135 32 789 32 1556 32 42
Cuts at: 4023 4055 1373
Size: 32 2626
Fragments arranged by size:
2626 1556 789 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2652 2898 669
Size: 988 281 714 246 3079
Fragments arranged by size:
3079 988 714 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5308
HpaI GTT'AAC
Cuts at: 2898 2898
Size: 5308
KpnI G_GTAC'C
Cuts at: 2251 2633 2251
Size: 382 4926
MfeI C'AATT_G
Cuts at: 2907 2907
Size: 5308
MluI A'CGCG_T
Cuts at: 5184 5184
Size: 5308
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4422 4606 1305
Size: 3117 184 2007
Fragments arranged by size:
3117 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3486
Fragments arranged by size:
3486 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2060 2427 3266 3625 3784 518
Size: 648 894 367 839 359 159 2042
Fragments arranged by size:
2042 894 839 648 367 359 159
MspA1I CmG'CkG
Cuts at: 1606 2157 2724 3401 4549 4794 1606
Size: 551 567 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 567 551 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4756
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5308
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5308
NotI GC'GGCC_GC
Cuts at: 2755 2755
Size: 5308
NruI TCG'CGA
Cuts at: 2226 2226
Size: 5308
NspI r_CATG'y
Cuts at: 1132 1229 2428 2666 4211 1132
Size: 97 1199 238 1545 2229
Fragments arranged by size:
2229 1545 1199 238 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5308
PciI A'CATG_T
Cuts at: 4207 4207
Size: 5308
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5308
PmeI GTTT'AAAC
Cuts at: 2612 2612
Size: 5308
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2093 2125 3377 3409 4890 4922 2093
Size: 32 1252 32 1481 32 2479
Fragments arranged by size:
2479 1481 1252 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2191 2191
Size: 5308
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5308
PsiI TTA'TAA
Cuts at: 2878 2878
Size: 5308
PstI C_TGCA'G
Cuts at: 830 2660 830
Size: 1830 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5308
SacI G_AGCT'C
Cuts at: 2326 2326
Size: 5308
SacII CC_GC'GG
Cuts at: 2158 2158
Size: 5308
SalI G'TCGA_C
Cuts at: 1936 2650 1936
Size: 714 4594
SbfI CC_TGCA'GG
Cuts at: 2660 2660
Size: 5308
ScaI AGT'ACT
Cuts at: 3556 5268 3556
Size: 1712 3596
SfcI C'TryA_G
Cuts at: 826 1045 2656 3791 4472 4663 826
Size: 219 1611 1135 681 191 1471
Fragments arranged by size:
1611 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5308
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5308
SmaI CCC'GGG
Cuts at: 1872 2635 1872
Size: 763 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2144 3412 4313 4575 820
Size: 197 926 201 1268 901 262 1553
Fragments arranged by size:
1553 1268 926 901 262 201 197
SnaBI TAC'GTA
Cuts at: 493 3099 493
Size: 2606 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5308
SphI G_CATG'C
Cuts at: 1132 2666 1132
Size: 1534 3774
SspI AAT'ATT
Cuts at: 5 52 3232 5
Size: 47 3180 2081
Fragments arranged by size:
3180 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5308
StyI C'CwwG_G
Cuts at: 513 1065 2091 2747 513
Size: 552 1026 656 3074
Fragments arranged by size:
3074 1026 656 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3335 3352 3505 3690 4135 325
Size: 1255 1755 17 153 185 445 1498
Fragments arranged by size:
1755 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3554 5266 96
Size: 274 80 33 51 1443 1577 1712 138
Fragments arranged by size:
1712 1577 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2004 2036 5268 5300 2004
Size: 32 3232 32 2012
Fragments arranged by size:
3232 2012 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5308
XbaI T'CTAG_A
Cuts at: 2644 2644
Size: 5308
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2400 2400
Size: 5308
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5308
XmaI C'CCGG_G
Cuts at: 1870 2633 1870
Size: 763 4545
XmnI GAAnn'nnTTC
Cuts at: 3437 3437
Size: 5308
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3116 276
Size: 53 83 186 936 1582 2468
Fragments arranged by size:
2468 1582 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NruI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SacI SacII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI