Restriction map for
pFN21AB4677
(Circular) (Six-base) MAPSORT of: pFN21AB4677.seq Check: 9859 from: 1 to: 7618
pFN21AB4677 7618 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2086 5428 278
Size: 53 83 186 936 550 3342 2468
Fragments arranged by size:
3342 2468 936 550 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4441 4586 4811 4961 1937
Size: 2504 145 225 150 4594
Fragments arranged by size:
4594 2504 225 150 145
Acc65I G'GTAC_C
Cuts at: 4887 4939 4887
Size: 52 7566
AclI AA'CG_TT
Cuts at: 1563 5745 6118 1563
Size: 4182 373 3063
Fragments arranged by size:
4182 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2175 2955 3687 4058 4322 4877 5681 7065
Size: 543 780 732 371 264 555 804 1384
Cuts at: 7065 7333 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 804 780 732 555 543 371
268 264
AfeI AGC'GCT
Cuts at: 4869 4869
Size: 7618
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7421
AflIII A'CryG_T
Cuts at: 1203 3855 6517 7494 1203
Size: 2652 2662 977 1327
Fragments arranged by size:
2662 2652 1327 977
AgeI A'CCGG_T
Cuts at: 6449 6449
Size: 7618
AhdI GACnn_n'nnGTC
Cuts at: 4039 6347 4039
Size: 2308 5310
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7618
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7618
AlwNI CAG_nnn'CTG
Cuts at: 4213 5361 6933 4213
Size: 1148 1572 4898
Fragments arranged by size:
4898 1572 1148
ApaLI G'TGCA_C
Cuts at: 2294 5675 6831 2294
Size: 3381 1156 3081
Fragments arranged by size:
3381 3081 1156
ApoI r'AATT_y
Cuts at: 1493 2096 2253 2322 2492 2690 2858 2899 3062
Size: 603 157 69 170 198 168 41 163
Cuts at: 3062 3431 3919 3977 4182 4342 4491 4779 4927
Size: 369 488 58 205 160 149 288 148
Cuts at: 4927 5153 5305 7414 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 603 488 369 288 226 205
198 170 168 163 160 157 152 149
148 69 58 41
AseI AT'TA_AT
Cuts at: 160 1032 2442 3528 4386 6172 160
Size: 872 1410 1086 858 1786 1606
Fragments arranged by size:
1786 1606 1410 1086 872 858
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7618
AvaI C'yCGr_G
Cuts at: 1870 1943 4943 1870
Size: 73 3000 4545
Fragments arranged by size:
4545 3000 73
BamHI G'GATC_C
Cuts at: 4948 5315 4948
Size: 367 7251
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4887 4939 6394 618
Size: 325 217 627 3100 52 1455 1842
Fragments arranged by size:
3100 1842 1455 627 325 217 52
BanII G_rGCy'C
Cuts at: 1391 2802 4937 1391
Size: 1411 2135 4072
Fragments arranged by size:
4072 2135 1411
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4035 928
Size: 971 2136 4511
Fragments arranged by size:
4511 2136 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3165 3199 5809 5843 1903
Size: 34 1228 34 2610 34 3678
Fragments arranged by size:
3678 2610 1228 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5510 6720 5510
Size: 1210 6408
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7618
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6229 136
Size: 107 122 71 631 605 4557 1525
Fragments arranged by size:
4557 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4524 7613 4524
Size: 3089 4529
BlpI GC'TnA_GC
Cuts at: 2306 5035 2306
Size: 2729 4889
Bme1580I G_kGCm'C
Cuts at: 1790 2298 4414 5679 6835 1790
Size: 508 2116 1265 1156 2573
Fragments arranged by size:
2573 2116 1265 1156 508
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7618
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5349 6307 442
Size: 416 53 83 399 24 3932 958 1753
Fragments arranged by size:
3932 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7618
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2174 2245 4856 6278 1239
Size: 935 71 2611 1422 2579
Fragments arranged by size:
2611 2579 1422 935 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5743 6608 6906 5743
Size: 865 298 6455
Fragments arranged by size:
6455 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2977 6281 882
Size: 617 1478 3304 2219
Fragments arranged by size:
3304 2219 1478 617
BsaAI yAC'GTr
Cuts at: 493 1204 5409 493
Size: 711 4205 2702
Fragments arranged by size:
4205 2702 711
BsaBI GATnn'nnATC
Cuts at: 2022 2067 7612 2022
Size: 45 5545 2028
Fragments arranged by size:
5545 2028 45
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2083 5425 5807 6442
Size: 53 83 186 936 550 3342 382 635
Cuts at: 6442 275
Size: 1451
Fragments arranged by size:
3342 1451 936 635 550 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6051 6449 6723 6870 7281 6051
Size: 398 274 147 411 6388
Fragments arranged by size:
6388 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3920 3950 1178
Size: 30 165 30 2517 30 4846
Fragments arranged by size:
4846 2517 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3119 3941 1186
Size: 1933 822 4863
Fragments arranged by size:
4863 1933 822
BseYI C'CCAG_C
Cuts at: 4730 6821 7317 4730
Size: 2091 496 5031
Fragments arranged by size:
5031 2091 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7045
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2269 5068 5829 6857 1540
Size: 275 186 268 2799 761 1028 2301
Fragments arranged by size:
2799 2301 1028 761 275 268 186
BsiHKAI G_wGCw'C
Cuts at: 2298 2802 4275 4850 5679 5764 6835 2298
Size: 504 1473 575 829 85 1071 3081
Fragments arranged by size:
3081 1473 1071 829 575 504 85
BsmI GAATG_Cn'
Cuts at: 4133 4864 5129 5222 4133
Size: 731 265 93 6529
Fragments arranged by size:
6529 731 265 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2298 2802 4275 4414 4850 4937 5679
Size: 399 508 504 1473 139 436 87 742
Cuts at: 5679 5764 6835 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1473 1071 742 508 504 436 399
139 87 85
BspEI T'CCGG_A
Cuts at: 7281 7281
Size: 7618
BspHI T'CATG_A
Cuts at: 2488 3732 5507 7237 2488
Size: 1244 1775 1730 2869
Fragments arranged by size:
2869 1775 1730 1244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2880 4973 844
Size: 2036 2093 3489
Fragments arranged by size:
3489 2093 2036
BsrBI CCG'CTC
Cuts at: 1394 1823 5505 1394
Size: 429 3682 3507
Fragments arranged by size:
3682 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6113 6287 65
Size: 1183 4865 174 1396
Fragments arranged by size:
4865 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2786 6262 6449 1700
Size: 110 976 3476 187 2869
Fragments arranged by size:
3476 2869 976 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7618
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7618
BssSI C'ACGA_G
Cuts at: 5678 6690 5678
Size: 1012 6606
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2302 2606 2837 3575 4169 794
Size: 1508 304 231 738 594 4243
Fragments arranged by size:
4243 1508 738 594 304 231
BstBI TT'CG_AA
Cuts at: 6422 6422
Size: 7618
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7618
BstXI CCAn_nnnn'nTGG
Cuts at: 2382 4086 2382
Size: 1704 5914
BstYI r'GATC_y
Cuts at: 1972 2680 3131 4524 4948 5315 5700 5717 7169
Size: 708 451 1393 424 367 385 17 1452
Cuts at: 7169 7255 7267 7613 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1393 708 451 424 385 367
346 86 17 12
BstZ17I GTA'TAC
Cuts at: 4442 4587 4442
Size: 145 7473
Bsu36I CC'TnA_GG
Cuts at: 2618 2618
Size: 7618
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6127
Fragments arranged by size:
6127 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2031 487
Size: 683 56 805 6074
Fragments arranged by size:
6074 805 683 56
BtsI GCAGTG_nn'
Cuts at: 4910 5134 5928 5948 4910
Size: 224 794 20 6580
Fragments arranged by size:
6580 794 224 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7618
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7583
DraI TTT'AAA
Cuts at: 2049 2476 3411 4922 2049
Size: 427 935 1511 4745
Fragments arranged by size:
4745 1511 935 427
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6625 809
Size: 507 5309 1802
Fragments arranged by size:
5309 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2274 5065 5954
Size: 54 1425 50 275 18 444 2791 889
Cuts at: 5954 7583 8
Size: 1629 43
Fragments arranged by size:
2791 1629 1425 889 444 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5065 1537
Size: 275 3253 4090
Fragments arranged by size:
4090 3253 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2772 3706 5548 1353
Size: 1419 934 1842 3423
Fragments arranged by size:
3423 1842 1419 934
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6205 6579 6725 387
Size: 1064 138 4616 374 146 1280
Fragments arranged by size:
4616 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2800 2800
Size: 7618
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2174 2175 2245 2955 3687 4058 4322
Size: 393 542 1 70 710 732 371 264
Cuts at: 4322 4856 4877 5681 6278 7065 7333 1239
Size: 534 21 804 597 787 268 1524
Fragments arranged by size:
1524 804 787 732 710 597 542 534
393 371 268 264 70 21 1
EcoNI CCTnn'n_nnAGG
Cuts at: 3207 3207
Size: 7618
EcoO109I rG'GnC_Cy
Cuts at: 3200 3605 3200
Size: 405 7213
EcoRI G'AATT_C
Cuts at: 2858 4342 4927 5305 2858
Size: 1484 585 378 5171
Fragments arranged by size:
5171 1484 585 378
EcoRV GAT'ATC
Cuts at: 4700 4700
Size: 7618
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3518 3906 4907 3518
Size: 388 1001 6229
Fragments arranged by size:
6229 1001 388
FspI TGC'GCA
Cuts at: 6124 6124
Size: 7618
HaeII r_GCGC'y
Cuts at: 1678 4871 6765 1678
Size: 3193 1894 2531
Fragments arranged by size:
3193 2531 1894
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3398 3430 3517 3549 4025
Size: 32 135 32 1826 32 87 32 476
Cuts at: 4025 4057 6259 6291 6333 6365 1373
Size: 32 2202 32 42 32 2626
Fragments arranged by size:
2626 2202 1826 476 135 87 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3271 4294 4962 5208 669
Size: 988 281 1333 1023 668 246 3079
Fragments arranged by size:
3079 1333 1023 988 668 281 246
HindIII A'AGCT_T
Cuts at: 748 2525 2756 748
Size: 1777 231 5610
Fragments arranged by size:
5610 1777 231
HpaI GTT'AAC
Cuts at: 5208 5208
Size: 7618
KpnI G_GTAC'C
Cuts at: 4891 4943 4891
Size: 52 7566
MfeI C'AATT_G
Cuts at: 5217 5217
Size: 7618
MluI A'CGCG_T
Cuts at: 7494 7494
Size: 7618
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2104 6732 6916 1305
Size: 799 4628 184 2007
Fragments arranged by size:
4628 2007 799 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2276 10
Size: 54 1425 343 444 5352
Fragments arranged by size:
5352 1425 444 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2380 2601 3285 4029 5576 5935 6094
Size: 648 1214 221 684 744 1547 359 159
Cuts at: 6094 518
Size: 2042
Fragments arranged by size:
2042 1547 1214 744 684 648 359 221
159
MspA1I CmG'CkG
Cuts at: 1606 2090 3632 4175 5034 5711 6859 7104 1606
Size: 484 1542 543 859 677 1148 245 2120
Fragments arranged by size:
2120 1542 1148 859 677 543 484 245
NaeI GCC'GGC
Cuts at: 2788 2788
Size: 7618
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6127
Fragments arranged by size:
6127 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7618
NgoMIV G'CCGG_C
Cuts at: 2786 2786
Size: 7618
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7618
NotI GC'GGCC_GC
Cuts at: 5065 5065
Size: 7618
NspI r_CATG'y
Cuts at: 1132 1229 3859 4279 4976 6521 1132
Size: 97 2630 420 697 1545 2229
Fragments arranged by size:
2630 2229 1545 697 420 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7618
PciI A'CATG_T
Cuts at: 3855 6517 3855
Size: 2662 4956
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4596 1108
Size: 3488 4130
PfoI T'CCnGG_A
Cuts at: 4822 4822
Size: 7618
PmeI GTTT'AAAC
Cuts at: 4922 4922
Size: 7618
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5687 5719 7200 7232 5687
Size: 32 1481 32 6073
Fragments arranged by size:
6073 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3200 3200
Size: 7618
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7618
PsiI TTA'TAA
Cuts at: 2994 5188 2994
Size: 2194 5424
PstI C_TGCA'G
Cuts at: 830 2591 3246 3637 4174 4970 830
Size: 1761 655 391 537 796 3478
Fragments arranged by size:
3478 1761 796 655 537 391
PvuI CG_AT'CG
Cuts at: 2001 2269 2001
Size: 268 7350
PvuII CAG'CTG
Cuts at: 2090 3632 4175 2090
Size: 1542 543 5533
Fragments arranged by size:
5533 1542 543
SacI G_AGCT'C
Cuts at: 2802 2802
Size: 7618
SalI G'TCGA_C
Cuts at: 1936 4960 1936
Size: 3024 4594
SapI GCTCTTCn'nnn_
Cuts at: 3706 3706
Size: 7618
SbfI CC_TGCA'GG
Cuts at: 4970 4970
Size: 7618
ScaI AGT'ACT
Cuts at: 5866 7578 5866
Size: 1712 5906
SfcI C'TryA_G
Cuts at: 826 1045 2587 2662 3242 3633 4170 4752 4966
Size: 219 1542 75 580 391 537 582 214
Cuts at: 4966 6101 6782 6973 826
Size: 1135 681 191 1471
Fragments arranged by size:
1542 1471 1135 681 582 580 537 391
219 214 191 75
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7618
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7618
SmaI CCC'GGG
Cuts at: 1872 4945 1872
Size: 3073 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5722 6623 6885 820
Size: 197 926 3779 901 262 1553
Fragments arranged by size:
3779 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5409 493
Size: 4916 2702
SpeI A'CTAG_T
Cuts at: 152 2905 152
Size: 2753 4865
SphI G_CATG'C
Cuts at: 1132 4976 1132
Size: 3844 3774
SspI AAT'ATT
Cuts at: 5 52 2045 4105 4431 5542 5
Size: 47 1993 2060 326 1111 2081
Fragments arranged by size:
2081 2060 1993 1111 326 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7618
StyI C'CwwG_G
Cuts at: 513 1065 2004 4240 4882 5057 513
Size: 552 939 2236 642 175 3074
Fragments arranged by size:
3074 2236 939 642 552 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5645 5662 5815 6000 6445 325
Size: 1255 4065 17 153 185 445 1498
Fragments arranged by size:
4065 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3108 5864 7576
Size: 274 80 33 51 1443 1131 2756 1712
Cuts at: 7576 96
Size: 138
Fragments arranged by size:
2756 1712 1443 1131 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7578 7610 7578
Size: 32 7586
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7618
XbaI T'CTAG_A
Cuts at: 4954 4954
Size: 7618
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2441 3270 4783 2441
Size: 829 1513 5276
Fragments arranged by size:
5276 1513 829
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7618
XmaI C'CCGG_G
Cuts at: 1870 4943 1870
Size: 3073 4545
XmnI GAAnn'nnTTC
Cuts at: 2370 3742 3981 5747 2370
Size: 1372 239 1766 4241
Fragments arranged by size:
4241 1766 1372 239
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2084 5426 276
Size: 53 83 186 936 550 3342 2468
Fragments arranged by size:
3342 2468 936 550 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI DraIII FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI