Restriction map for
pFN21AB4675
(Circular) (Six-base) MAPSORT of: pFN21AB4675.seq Check: 5701 from: 1 to: 6187
pFN21AB4675 6187 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2405 2405
Size: 6187
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3997 278
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3530 1937
Size: 1593 4594
Acc65I G'GTAC_C
Cuts at: 3508 3508
Size: 6187
AclI AA'CG_TT
Cuts at: 1563 2915 4314 4687 1563
Size: 1352 1399 373 3063
Fragments arranged by size:
3063 1399 1352 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2415 2430 2805 2807 2870 3285 4250 5634
Size: 783 15 375 2 63 415 965 1384
Cuts at: 5634 5902 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 965 783 415 375 268 63
15 2
AflII C'TTAA_G
Cuts at: 820 1017 3035 820
Size: 197 2018 3972
Fragments arranged by size:
3972 2018 197
AflIII A'CryG_T
Cuts at: 1203 5086 6063 1203
Size: 3883 977 1327
Fragments arranged by size:
3883 1327 977
AgeI A'CCGG_T
Cuts at: 5018 5018
Size: 6187
AhdI GACnn_n'nnGTC
Cuts at: 4916 4916
Size: 6187
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6187
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2943 1184
Size: 1759 4428
AlwNI CAG_nnn'CTG
Cuts at: 2783 3930 5502 2783
Size: 1147 1572 3468
Fragments arranged by size:
3468 1572 1147
ApaLI G'TGCA_C
Cuts at: 4244 5400 4244
Size: 1156 5031
ApoI r'AATT_y
Cuts at: 1493 2045 3047 3122 3496 3722 3874 5983 1493
Size: 552 1002 75 374 226 152 2109 1697
Fragments arranged by size:
2109 1697 1002 552 374 226 152 75
AseI AT'TA_AT
Cuts at: 160 1032 4741 160
Size: 872 3709 1606
Fragments arranged by size:
3709 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
AvaI C'yCGr_G
Cuts at: 1870 1943 3512 1870
Size: 73 1569 4545
Fragments arranged by size:
4545 1569 73
BamHI G'GATC_C
Cuts at: 3517 3884 3517
Size: 367 5820
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2417 3508 4963 618
Size: 325 217 627 630 1091 1455 1842
Fragments arranged by size:
1842 1455 1091 630 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3506 1391
Size: 2115 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2257 2280 928
Size: 971 358 23 4835
Fragments arranged by size:
4835 971 358 23
BbvCI CC'TCA_GC
Cuts at: 2251 2251
Size: 6187
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4378 4412 1903
Size: 34 50 34 2357 34 3678
Fragments arranged by size:
3678 2357 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2610 3245 4079 5289 2610
Size: 635 834 1210 3508
Fragments arranged by size:
3508 1210 834 635
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6187
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4798 136
Size: 107 122 71 631 605 3126 1525
Fragments arranged by size:
3126 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6182 6182
Size: 6187
BlpI GC'TnA_GC
Cuts at: 2097 2828 3604 2097
Size: 731 776 4680
Fragments arranged by size:
4680 776 731
Bme1580I G_kGCm'C
Cuts at: 1790 4248 5404 1790
Size: 2458 1156 2573
Fragments arranged by size:
2573 2458 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6187
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3918 4876 442
Size: 416 53 83 399 24 2501 958 1753
Fragments arranged by size:
2501 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6187
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2701 2701
Size: 6187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2136 4847 1239
Size: 897 2711 2579
Fragments arranged by size:
2711 2579 897
Bpu10I CC'TnA_GC
Cuts at: 2251 2251
Size: 6187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2443 4312 5177 5475 2443
Size: 1869 865 298 3155
Fragments arranged by size:
3155 1869 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4850 882
Size: 617 3351 2219
Fragments arranged by size:
3351 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3978 493
Size: 711 2774 2702
Fragments arranged by size:
2774 2702 711
BsaBI GATnn'nnATC
Cuts at: 6181 6181
Size: 6187
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3994 4376 5011 275
Size: 53 83 186 936 2461 382 635 1451
Fragments arranged by size:
2461 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4620 5018 5292 5439 5850 4620
Size: 398 274 147 411 4957
Fragments arranged by size:
4957 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5962
Fragments arranged by size:
5962 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2677 1186
Size: 1491 4696
BseYI C'CCAG_C
Cuts at: 5390 5886 5390
Size: 496 5691
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5614
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3637 4398 5426 1540
Size: 275 186 1636 761 1028 2301
Fragments arranged by size:
2301 1636 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4248 4333 5404 4248
Size: 85 1071 5031
Fragments arranged by size:
5031 1071 85
BsmI GAATG_Cn'
Cuts at: 2359 3028 3698 3791 2359
Size: 669 670 93 4755
Fragments arranged by size:
4755 670 669 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3506 4248 4333 5404 1391
Size: 399 1716 742 85 1071 2174
Fragments arranged by size:
2174 1716 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5850 5850
Size: 6187
BspHI T'CATG_A
Cuts at: 4076 5806 4076
Size: 1730 4457
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2405 3542 844
Size: 1561 1137 3489
Fragments arranged by size:
3489 1561 1137
BsrBI CCG'CTC
Cuts at: 1394 1823 2011 4074 1394
Size: 429 188 2063 3507
Fragments arranged by size:
3507 2063 429 188
BsrDI GCAATG_nn'
Cuts at: 65 1248 4682 4856 65
Size: 1183 3434 174 1396
Fragments arranged by size:
3434 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2958 4831 5018 1700
Size: 110 1148 1873 187 2869
Fragments arranged by size:
2869 1873 1148 187 110
BsrGI T'GTAC_A
Cuts at: 96 2774 3080 96
Size: 2678 306 3203
Fragments arranged by size:
3203 2678 306
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6187
BssSI C'ACGA_G
Cuts at: 4247 5259 4247
Size: 1012 5175
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2539 794
Size: 1745 4442
BstBI TT'CG_AA
Cuts at: 4991 4991
Size: 6187
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6187
BstXI CCAn_nnnn'nTGG
Cuts at: 2986 2986
Size: 6187
BstYI r'GATC_y
Cuts at: 1972 3130 3517 3884 4269 4286 5738 5824 5836
Size: 1158 387 367 385 17 1452 86 12
Cuts at: 5836 6182 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1158 387 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2490 513
Size: 552 1425 4210
Fragments arranged by size:
4210 1425 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5448
Fragments arranged by size:
5448 683 56
BtsI GCAGTG_nn'
Cuts at: 3703 4497 4517 3703
Size: 794 20 5373
Fragments arranged by size:
5373 794 20
ClaI AT'CG_AT
Cuts at: 1554 2733 1554
Size: 1179 5008
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3229 3264 529
Size: 35 2665 35 3452
Fragments arranged by size:
3452 2665 35 35
DraI TTT'AAA
Cuts at: 3491 3491
Size: 6187
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5194 809
Size: 507 3878 1802
Fragments arranged by size:
3878 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3634 4523 6152
Size: 54 1425 50 275 18 1804 889 1629
Cuts at: 6152 8
Size: 43
Fragments arranged by size:
1804 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3634 1537
Size: 275 1822 4090
Fragments arranged by size:
4090 1822 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2241 3465 4117 1353
Size: 888 1224 652 3423
Fragments arranged by size:
3423 1224 888 652
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4774 5148 5294 387
Size: 1064 138 3185 374 146 1280
Fragments arranged by size:
3185 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2136 2415 2430 2805 2807 2870 3285
Size: 393 504 279 15 375 2 63 415
Cuts at: 3285 4250 4847 5634 5902 1239
Size: 965 597 787 268 1524
Fragments arranged by size:
1524 965 787 597 504 415 393 375
279 268 63 15 2
EcoRI G'AATT_C
Cuts at: 3496 3874 3496
Size: 378 5809
EcoRV GAT'ATC
Cuts at: 3391 3391
Size: 6187
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2417 2417
Size: 6187
FspI TGC'GCA
Cuts at: 4693 4693
Size: 6187
HaeII r_GCGC'y
Cuts at: 1678 5334 1678
Size: 3656 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2675 2707 2999 3031 3221
Size: 32 135 32 1103 32 292 32 190
Cuts at: 3221 3253 4828 4860 4902 4934 1373
Size: 32 1575 32 42 32 2626
Fragments arranged by size:
2626 1575 1103 292 190 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2112 3531 3777 669
Size: 988 281 174 1419 246 3079
Fragments arranged by size:
3079 1419 988 281 246 174
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6187
HpaI GTT'AAC
Cuts at: 3777 3777
Size: 6187
KpnI G_GTAC'C
Cuts at: 3512 3512
Size: 6187
MfeI C'AATT_G
Cuts at: 3786 3786
Size: 6187
MluI A'CGCG_T
Cuts at: 6063 6063
Size: 6187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2594 2936 3042 5301 5485 1305
Size: 1289 342 106 2259 184 2007
Fragments arranged by size:
2259 2007 1289 342 184 106
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4365
Fragments arranged by size:
4365 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2984 4145 4504 4663 518
Size: 648 1818 1161 359 159 2042
Fragments arranged by size:
2042 1818 1161 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2101 3603 4280 5428 5673 1606
Size: 495 1502 677 1148 245 2120
Fragments arranged by size:
2120 1502 1148 677 495 245
NaeI GCC'GGC
Cuts at: 2960 2960
Size: 6187
NcoI C'CATG_G
Cuts at: 513 1065 2490 513
Size: 552 1425 4210
Fragments arranged by size:
4210 1425 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6187
NgoMIV G'CCGG_C
Cuts at: 2958 2958
Size: 6187
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6187
NotI GC'GGCC_GC
Cuts at: 3634 3634
Size: 6187
NspI r_CATG'y
Cuts at: 1132 1229 3545 5090 1132
Size: 97 2316 1545 2229
Fragments arranged by size:
2316 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6187
PciI A'CATG_T
Cuts at: 5086 5086
Size: 6187
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6187
PmeI GTTT'AAAC
Cuts at: 3491 3491
Size: 6187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4256 4288 5769 5801 4256
Size: 32 1481 32 4642
Fragments arranged by size:
4642 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2117 1613
Size: 504 5683
PsiI TTA'TAA
Cuts at: 3003 3757 3003
Size: 754 5433
PstI C_TGCA'G
Cuts at: 830 2467 2535 3539 830
Size: 1637 68 1004 3478
Fragments arranged by size:
3478 1637 1004 68
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6187
PvuII CAG'CTG
Cuts at: 2101 2101
Size: 6187
SalI G'TCGA_C
Cuts at: 1936 3529 1936
Size: 1593 4594
SapI GCTCTTCn'nnn_
Cuts at: 2241 2241
Size: 6187
SbfI CC_TGCA'GG
Cuts at: 3539 3539
Size: 6187
ScaI AGT'ACT
Cuts at: 3273 4435 6147 3273
Size: 1162 1712 3313
Fragments arranged by size:
3313 1712 1162
SfcI C'TryA_G
Cuts at: 826 1045 2463 2531 3535 4670 5351 5542 826
Size: 219 1418 68 1004 1135 681 191 1471
Fragments arranged by size:
1471 1418 1135 1004 681 219 191 68
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6187
SmaI CCC'GGG
Cuts at: 1872 3514 1872
Size: 1642 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2422 3035 4291 5192 5454 820
Size: 197 926 479 613 1256 901 262 1553
Fragments arranged by size:
1553 1256 926 901 613 479 262 197
SnaBI TAC'GTA
Cuts at: 493 3978 493
Size: 3485 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6187
SphI G_CATG'C
Cuts at: 1132 3545 1132
Size: 2413 3774
SspI AAT'ATT
Cuts at: 5 52 4111 5
Size: 47 4059 2081
Fragments arranged by size:
4059 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6187
StyI C'CwwG_G
Cuts at: 513 1065 2332 2490 3626 513
Size: 552 1267 158 1136 3074
Fragments arranged by size:
3074 1267 1136 552 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3310 4214 4231 4384 4569 5014 325
Size: 1255 1730 904 17 153 185 445 1498
Fragments arranged by size:
1730 1498 1255 904 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2774 3080 3271
Size: 274 80 33 51 1443 797 306 191
Cuts at: 3271 4433 6145 96
Size: 1162 1712 138
Fragments arranged by size:
1712 1443 1162 797 306 274 191 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6147 6179 6147
Size: 32 6155
Tth111I GACn'n_nGTC
Cuts at: 1316 3365 1316
Size: 2049 4138
XbaI T'CTAG_A
Cuts at: 3133 3523 3133
Size: 390 5797
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6187
XmaI C'CCGG_G
Cuts at: 1870 3512 1870
Size: 1642 4545
XmnI GAAnn'nnTTC
Cuts at: 4316 4316
Size: 6187
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3995 276
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI