Restriction map for
pFN21AB4674
(Circular) (Six-base) MAPSORT of: pFN21AB4674.seq Check: 899 from: 1 to: 5881
pFN21AB4674 5881 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2143 2143
Size: 5881
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3691 278
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3224 1937
Size: 1287 4594
Acc65I G'GTAC_C
Cuts at: 3202 3202
Size: 5881
AclI AA'CG_TT
Cuts at: 1563 4008 4381 1563
Size: 2445 373 3063
Fragments arranged by size:
3063 2445 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2625 3944 5328 5596 1632
Size: 993 1319 1384 268 1917
Fragments arranged by size:
1917 1384 1319 993 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5684
AflIII A'CryG_T
Cuts at: 1203 4780 5757 1203
Size: 3577 977 1327
Fragments arranged by size:
3577 1327 977
AgeI A'CCGG_T
Cuts at: 4712 4712
Size: 5881
AhdI GACnn_n'nnGTC
Cuts at: 4610 4610
Size: 5881
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5881
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5881
AlwNI CAG_nnn'CTG
Cuts at: 2060 3624 5196 2060
Size: 1564 1572 2745
Fragments arranged by size:
2745 1572 1564
ApaLI G'TGCA_C
Cuts at: 3938 5094 3938
Size: 1156 4725
ApoI r'AATT_y
Cuts at: 1493 2442 3190 3416 3568 5677 1493
Size: 949 748 226 152 2109 1697
Fragments arranged by size:
2109 1697 949 748 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4435 160
Size: 872 3403 1606
Fragments arranged by size:
3403 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
AvaI C'yCGr_G
Cuts at: 1870 1943 2190 3206 1870
Size: 73 247 1016 4545
Fragments arranged by size:
4545 1016 247 73
BamHI G'GATC_C
Cuts at: 3211 3578 3211
Size: 367 5514
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3202 4657 618
Size: 325 217 627 1415 1455 1842
Fragments arranged by size:
1842 1455 1415 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2190 2584 3200 1391
Size: 799 394 616 4072
Fragments arranged by size:
4072 799 616 394
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4910
BbvCI CC'TCA_GC
Cuts at: 2061 2061
Size: 5881
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2057 2091 4072 4106 1903
Size: 34 120 34 1981 34 3678
Fragments arranged by size:
3678 1981 120 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3773 4983 3773
Size: 1210 4671
BclI T'GATC_A
Cuts at: 1548 2476 2961 3171 1548
Size: 928 485 210 4258
Fragments arranged by size:
4258 928 485 210
BfrBI ATG'CAT
Cuts at: 2688 2688
Size: 5881
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4492 136
Size: 107 122 71 631 605 2820 1525
Fragments arranged by size:
2820 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5876 5876
Size: 5881
BlpI GC'TnA_GC
Cuts at: 3298 3298
Size: 5881
Bme1580I G_kGCm'C
Cuts at: 1790 2070 2840 3942 5098 1790
Size: 280 770 1102 1156 2573
Fragments arranged by size:
2573 1156 1102 770 280
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5881
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3612 4570 442
Size: 416 53 83 399 24 2195 958 1753
Fragments arranged by size:
2195 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5881
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2442 4541 1239
Size: 1203 2099 2579
Fragments arranged by size:
2579 2099 1203
Bpu10I CC'TnA_GC
Cuts at: 2045 2061 2045
Size: 16 5865
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4006 4871 5169 4006
Size: 865 298 4718
Fragments arranged by size:
4718 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4544 882
Size: 617 3045 2219
Fragments arranged by size:
3045 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3672 493
Size: 711 2468 2702
Fragments arranged by size:
2702 2468 711
BsaBI GATnn'nnATC
Cuts at: 2700 5875 2700
Size: 3175 2706
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2074 3688 4070 4705
Size: 53 83 186 936 541 1614 382 635
Cuts at: 4705 275
Size: 1451
Fragments arranged by size:
1614 1451 936 635 541 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2182 2550 4314 4712 4986 5133 5544 2182
Size: 368 1764 398 274 147 411 2519
Fragments arranged by size:
2519 1764 411 398 368 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5656
Fragments arranged by size:
5656 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5881
BseYI C'CCAG_C
Cuts at: 5084 5580 5084
Size: 496 5385
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5308
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3331 4092 5120 1540
Size: 275 186 1330 761 1028 2301
Fragments arranged by size:
2301 1330 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3942 4027 5098 3942
Size: 85 1071 4725
Fragments arranged by size:
4725 1071 85
BsmI GAATG_Cn'
Cuts at: 3392 3485 3392
Size: 93 5788
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2190 2584 2840 3200 3942 4027
Size: 399 280 120 394 256 360 742 85
Cuts at: 4027 5098 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 394 360 280 256
120 85
BspEI T'CCGG_A
Cuts at: 2182 5544 2182
Size: 3362 2519
BspHI T'CATG_A
Cuts at: 3770 5500 3770
Size: 1730 4151
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2143 3236 844
Size: 1299 1093 3489
Fragments arranged by size:
3489 1299 1093
BsrBI CCG'CTC
Cuts at: 1394 1823 3768 1394
Size: 429 1945 3507
Fragments arranged by size:
3507 1945 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2433 2620 4376 4550 65
Size: 1183 1185 187 1756 174 1396
Fragments arranged by size:
1756 1396 1185 1183 187 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2027 4525 4712 1700
Size: 110 217 2498 187 2869
Fragments arranged by size:
2869 2498 217 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5881
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5881
BssSI C'ACGA_G
Cuts at: 2990 3941 4953 2990
Size: 951 1012 3918
Fragments arranged by size:
3918 1012 951
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2615 794
Size: 1821 4060
BstBI TT'CG_AA
Cuts at: 3163 4685 3163
Size: 1522 4359
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5881
BstXI CCAn_nnnn'nTGG
Cuts at: 3141 3141
Size: 5881
BstYI r'GATC_y
Cuts at: 1972 2179 2266 2521 3211 3578 3963 3980 5432
Size: 207 87 255 690 367 385 17 1452
Cuts at: 5432 5518 5530 5876 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 690 385 367 346 255 207
87 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2871 513
Size: 552 939 867 3523
Fragments arranged by size:
3523 939 867 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5142
Fragments arranged by size:
5142 683 56
BtsI GCAGTG_nn'
Cuts at: 3397 4191 4211 3397
Size: 794 20 5067
Fragments arranged by size:
5067 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5881
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5846
DraI TTT'AAA
Cuts at: 3185 3185
Size: 5881
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4888 809
Size: 507 3572 1802
Fragments arranged by size:
3572 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2259 3328 4217
Size: 54 1425 50 275 18 429 1069 889
Cuts at: 4217 5846 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1069 889 429 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3328 1537
Size: 275 1516 4090
Fragments arranged by size:
4090 1516 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3811 1353
Size: 2458 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4468 4842 4988 387
Size: 1064 138 2879 374 146 1280
Fragments arranged by size:
2879 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2442 2625 3944 4541 5328 5596 1239
Size: 393 810 183 1319 597 787 268 1524
Fragments arranged by size:
1524 1319 810 787 597 393 268 183
EcoRI G'AATT_C
Cuts at: 3190 3568 3190
Size: 378 5503
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2693 2903 2693
Size: 210 5671
FspI TGC'GCA
Cuts at: 4387 4387
Size: 5881
HaeII r_GCGC'y
Cuts at: 1678 5028 1678
Size: 3350 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2388 2420 2967 2999 4522
Size: 32 135 32 816 32 547 32 1523
Cuts at: 4522 4554 4596 4628 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1523 816 547 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3225 3471 669
Size: 988 281 1287 246 3079
Fragments arranged by size:
3079 1287 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2885 748
Size: 2137 3744
HpaI GTT'AAC
Cuts at: 3471 3471
Size: 5881
KpnI G_GTAC'C
Cuts at: 3206 3206
Size: 5881
MfeI C'AATT_G
Cuts at: 3480 3480
Size: 5881
MluI A'CGCG_T
Cuts at: 5757 5757
Size: 5881
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2374 4995 5179 1305
Size: 1069 2621 184 2007
Fragments arranged by size:
2621 2007 1069 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2261 10
Size: 54 1425 343 429 3630
Fragments arranged by size:
3630 1425 429 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3839 4198 4357 518
Size: 648 2673 359 159 2042
Fragments arranged by size:
2673 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2747 3297 3974 5122 5367 1606
Size: 1141 550 677 1148 245 2120
Fragments arranged by size:
2120 1148 1141 677 550 245
NaeI GCC'GGC
Cuts at: 2029 2029
Size: 5881
NcoI C'CATG_G
Cuts at: 513 1065 2004 2871 513
Size: 552 939 867 3523
Fragments arranged by size:
3523 939 867 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5881
NgoMIV G'CCGG_C
Cuts at: 2027 2027
Size: 5881
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5881
NotI GC'GGCC_GC
Cuts at: 3328 3328
Size: 5881
NsiI A_TGCA'T
Cuts at: 2690 2690
Size: 5881
NspI r_CATG'y
Cuts at: 1132 1229 3239 4784 1132
Size: 97 2010 1545 2229
Fragments arranged by size:
2229 2010 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5881
PciI A'CATG_T
Cuts at: 4780 4780
Size: 5881
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5881
PmeI GTTT'AAAC
Cuts at: 3185 3185
Size: 5881
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3950 3982 5463 5495 3950
Size: 32 1481 32 4336
Fragments arranged by size:
4336 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5881
PsiI TTA'TAA
Cuts at: 3451 3451
Size: 5881
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2712 2744 2712
Size: 32 5849
PstI C_TGCA'G
Cuts at: 830 2278 3233 830
Size: 1448 955 3478
Fragments arranged by size:
3478 1448 955
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5881
SalI G'TCGA_C
Cuts at: 1936 3223 1936
Size: 1287 4594
SbfI CC_TGCA'GG
Cuts at: 3233 3233
Size: 5881
ScaI AGT'ACT
Cuts at: 4129 5841 4129
Size: 1712 4169
SfcI C'TryA_G
Cuts at: 826 1045 2274 2760 3033 3229 4364 5045 5236
Size: 219 1229 486 273 196 1135 681 191
Cuts at: 5236 826
Size: 1471
Fragments arranged by size:
1471 1229 1135 681 486 273 219 196
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5881
SmaI CCC'GGG
Cuts at: 1872 2192 3208 1872
Size: 320 1016 4545
Fragments arranged by size:
4545 1016 320
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3985 4886 5148 820
Size: 197 926 2042 901 262 1553
Fragments arranged by size:
2042 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3672 493
Size: 3179 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5881
SphI G_CATG'C
Cuts at: 1132 3239 1132
Size: 2107 3774
SspI AAT'ATT
Cuts at: 5 52 2283 3805 5
Size: 47 2231 1522 2081
Fragments arranged by size:
2231 2081 1522 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5881
StyI C'CwwG_G
Cuts at: 513 1065 2004 2244 2558 2871 2930 3320 513
Size: 552 939 240 314 313 59 390 3074
Fragments arranged by size:
3074 939 552 390 314 313 240 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3908 3925 4078 4263 4708 325
Size: 1255 2328 17 153 185 445 1498
Fragments arranged by size:
2328 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4127 5839 96
Size: 274 80 33 51 1443 2150 1712 138
Fragments arranged by size:
2150 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2622 2654 3088 3120 5841 5873 2622
Size: 32 434 32 2721 32 2630
Fragments arranged by size:
2721 2630 434 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5881
XbaI T'CTAG_A
Cuts at: 3217 3217
Size: 5881
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5881
XmaI C'CCGG_G
Cuts at: 1870 2190 3206 1870
Size: 320 1016 4545
Fragments arranged by size:
4545 1016 320
XmnI GAAnn'nnTTC
Cuts at: 2222 4010 2222
Size: 1788 4093
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3689 276
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FseI FspAI KasI NarI NruI PacI PfoI
PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI