Restriction map for
pFN21AB4670
(Circular) (Six-base) MAPSORT of: pFN21AB4670.seq Check: 8780 from: 1 to: 5908
pFN21AB4670 5908 bp
With 182 enzymes: SgfI *
October 20, 2009 10:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2343 3718 278
Size: 53 83 186 936 807 1375 2468
Fragments arranged by size:
2468 1375 936 807 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3251 1937
Size: 1314 4594
Acc65I G'GTAC_C
Cuts at: 3229 3229
Size: 5908
AclI AA'CG_TT
Cuts at: 1563 4035 4408 1563
Size: 2472 373 3063
Fragments arranged by size:
3063 2472 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3042 3971 5355 5623 1632
Size: 1410 929 1384 268 1917
Fragments arranged by size:
1917 1410 1384 929 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5711
AflIII A'CryG_T
Cuts at: 1203 4807 5784 1203
Size: 3604 977 1327
Fragments arranged by size:
3604 1327 977
AgeI A'CCGG_T
Cuts at: 4739 4739
Size: 5908
AhdI GACnn_n'nnGTC
Cuts at: 4637 4637
Size: 5908
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5908
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5908
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2043 2075 2043
Size: 32 5876
AlwNI CAG_nnn'CTG
Cuts at: 3651 5223 3651
Size: 1572 4336
ApaI G_GGCC'C
Cuts at: 2709 2709
Size: 5908
ApaLI G'TGCA_C
Cuts at: 3965 5121 3965
Size: 1156 4752
ApoI r'AATT_y
Cuts at: 1493 3217 3443 3595 5704 1493
Size: 1724 226 152 2109 1697
Fragments arranged by size:
2109 1724 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2988 4462 160
Size: 872 1956 1474 1606
Fragments arranged by size:
1956 1606 1474 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5908
AvaI C'yCGr_G
Cuts at: 1870 1943 2313 3233 1870
Size: 73 370 920 4545
Fragments arranged by size:
4545 920 370 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2894 2927 2894
Size: 33 5875
BamHI G'GATC_C
Cuts at: 2201 3138 3238 3605 2201
Size: 937 100 367 4504
Fragments arranged by size:
4504 937 367 100
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2250 2696 3229 4684 618
Size: 325 217 627 463 446 533 1455 1842
Fragments arranged by size:
1842 1455 627 533 463 446 325 217
BanII G_rGCy'C
Cuts at: 1391 2709 3227 1391
Size: 1318 518 4072
Fragments arranged by size:
4072 1318 518
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2371 928
Size: 971 472 4465
Fragments arranged by size:
4465 971 472
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4099 4133 1903
Size: 34 2162 34 3678
Fragments arranged by size:
3678 2162 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3800 5010 3800
Size: 1210 4698
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5908
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4519 136
Size: 107 122 71 631 605 2847 1525
Fragments arranged by size:
2847 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5903 5903
Size: 5908
BlpI GC'TnA_GC
Cuts at: 3325 3325
Size: 5908
Bme1580I G_kGCm'C
Cuts at: 1790 2253 2709 3969 5125 1790
Size: 463 456 1260 1156 2573
Fragments arranged by size:
2573 1260 1156 463 456
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5908
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3639 4597 442
Size: 416 53 83 399 24 2222 958 1753
Fragments arranged by size:
2222 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5908
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2903 2903
Size: 5908
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2343 2695 4568 1239
Size: 1104 352 1873 2579
Fragments arranged by size:
2579 1873 1104 352
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4033 4898 5196 4033
Size: 865 298 4745
Fragments arranged by size:
4745 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2073 2655 4571 882
Size: 617 574 582 1916 2219
Fragments arranged by size:
2219 1916 617 582 574
BsaAI yAC'GTr
Cuts at: 493 1204 3699 493
Size: 711 2495 2702
Fragments arranged by size:
2702 2495 711
BsaBI GATnn'nnATC
Cuts at: 2808 5902 2808
Size: 3094 2814
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2217 2340 3715 4097
Size: 53 83 186 936 684 123 1375 382
Cuts at: 4097 4732 275
Size: 635 1451
Fragments arranged by size:
1451 1375 936 684 635 382 186 123
83 53
BsaWI w'CCGG_w
Cuts at: 2812 4341 4739 5013 5160 5571 2812
Size: 1529 398 274 147 411 3149
Fragments arranged by size:
3149 1529 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2043 2073 2293 2323 2330
Size: 30 165 30 640 30 220 30 7
Cuts at: 2330 2360 2573 2603 1178
Size: 30 213 30 4483
Fragments arranged by size:
4483 640 220 213 165 30 30 30
30 30 30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2338 2611 1186
Size: 1152 273 4483
Fragments arranged by size:
4483 1152 273
BseYI C'CCAG_C
Cuts at: 5111 5607 5111
Size: 496 5412
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2290 1163
Size: 573 554 4781
Fragments arranged by size:
4781 573 554
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2099 3358 4119 5147 1540
Size: 275 186 98 1259 761 1028 2301
Fragments arranged by size:
2301 1259 1028 761 275 186 98
BsiHKAI G_wGCw'C
Cuts at: 3969 4054 5125 3969
Size: 85 1071 4752
Fragments arranged by size:
4752 1071 85
BsmI GAATG_Cn'
Cuts at: 3419 3512 3419
Size: 93 5815
BsmBI CGTCTCn'nnnn_
Cuts at: 2331 2574 2331
Size: 243 5665
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2253 2709 3227 3969 4054 5125 1391
Size: 399 463 456 518 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 518 463 456 399 85
BspEI T'CCGG_A
Cuts at: 2812 5571 2812
Size: 2759 3149
BspHI T'CATG_A
Cuts at: 2602 2874 3797 5527 2602
Size: 272 923 1730 2983
Fragments arranged by size:
2983 1730 923 272
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2312 2380 3029 3263 844
Size: 1468 68 649 234 3489
Fragments arranged by size:
3489 1468 649 234 68
BsrBI CCG'CTC
Cuts at: 1394 1823 2137 2736 3795 1394
Size: 429 314 599 1059 3507
Fragments arranged by size:
3507 1059 599 429 314
BsrDI GCAATG_nn'
Cuts at: 65 1248 3200 4403 4577 65
Size: 1183 1952 1203 174 1396
Fragments arranged by size:
1952 1396 1203 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2188 4552 4739 1700
Size: 110 378 2364 187 2869
Fragments arranged by size:
2869 2364 378 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5908
BssHII G'CGCG_C
Cuts at: 1925 2160 1925
Size: 235 5673
BssSI C'ACGA_G
Cuts at: 3968 4980 3968
Size: 1012 4896
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5908
BstBI TT'CG_AA
Cuts at: 4712 4712
Size: 5908
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5908
BstYI r'GATC_y
Cuts at: 1972 2201 3138 3238 3605 3990 4007 5459 5545
Size: 229 937 100 367 385 17 1452 86
Cuts at: 5545 5557 5903 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 937 385 367 346 229 100
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2093 2125 3099 513
Size: 552 939 89 32 974 3322
Fragments arranged by size:
3322 974 939 552 89 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2356 487
Size: 683 56 1130 4039
Fragments arranged by size:
4039 1130 683 56
BtsI GCAGTG_nn'
Cuts at: 2755 3424 4218 4238 2755
Size: 669 794 20 4425
Fragments arranged by size:
4425 794 669 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5908
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5873
DraI TTT'AAA
Cuts at: 3212 3212
Size: 5908
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2375 4915 809
Size: 507 1059 2540 1802
Fragments arranged by size:
2540 1802 1059 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2096 2190 3355
Size: 54 1425 50 275 18 266 94 1165
Cuts at: 3355 4244 5873 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1165 889 275 266 94 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2096 3355 1537
Size: 275 284 1259 4090
Fragments arranged by size:
4090 1259 284 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2894 2916 3838 1353
Size: 1541 22 922 3423
Fragments arranged by size:
3423 1541 922 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2349 4495 4869 5015 387
Size: 1064 138 760 2146 374 146 1280
Fragments arranged by size:
2146 1280 1064 760 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2343 2695 3042 3971 4568 5355 5623
Size: 393 711 352 347 929 597 787 268
Cuts at: 5623 1239
Size: 1524
Fragments arranged by size:
1524 929 787 711 597 393 352 347
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2793 2793
Size: 5908
EcoO109I rG'GnC_Cy
Cuts at: 2705 2706 3152 2705
Size: 1 446 5461
Fragments arranged by size:
5461 446 1
EcoRI G'AATT_C
Cuts at: 3217 3595 3217
Size: 378 5530
FspI TGC'GCA
Cuts at: 4414 4414
Size: 5908
HaeII r_GCGC'y
Cuts at: 1678 5055 1678
Size: 3377 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2193 2225 2293 2325 2330
Size: 32 135 32 621 32 68 32 5
Cuts at: 2330 2362 2573 2605 2663 2695 4549 4581 4623
Size: 32 211 32 58 32 1854 32 42
Cuts at: 4623 4655 1373
Size: 32 2626
Fragments arranged by size:
2626 1854 621 211 135 68 58 42
32 32 32 32 32 32 32 32
32 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2212 3252 3498 669
Size: 988 281 274 1040 246 3079
Fragments arranged by size:
3079 1040 988 281 274 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5908
HpaI GTT'AAC
Cuts at: 3498 3498
Size: 5908
KpnI G_GTAC'C
Cuts at: 3233 3233
Size: 5908
MfeI C'AATT_G
Cuts at: 3507 3507
Size: 5908
MluI A'CGCG_T
Cuts at: 5784 5784
Size: 5908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2376 3096 5022 5206 1305
Size: 1071 720 1926 184 2007
Fragments arranged by size:
2007 1926 1071 720 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4086
Fragments arranged by size:
4086 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2178 2670 3176 3866 4225 4384 518
Size: 648 1012 492 506 690 359 159 2042
Fragments arranged by size:
2042 1012 690 648 506 492 359 159
MspA1I CmG'CkG
Cuts at: 1606 2095 2207 3324 4001 5149 5394 1606
Size: 489 112 1117 677 1148 245 2120
Fragments arranged by size:
2120 1148 1117 677 489 245 112
NaeI GCC'GGC
Cuts at: 2190 2190
Size: 5908
NcoI C'CATG_G
Cuts at: 513 1065 2004 3099 513
Size: 552 939 1095 3322
Fragments arranged by size:
3322 1095 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5908
NgoMIV G'CCGG_C
Cuts at: 2188 2188
Size: 5908
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5908
NotI GC'GGCC_GC
Cuts at: 3355 3355
Size: 5908
NspI r_CATG'y
Cuts at: 1132 1229 3266 4811 1132
Size: 97 2037 1545 2229
Fragments arranged by size:
2229 2037 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2702 1348
Size: 1354 4554
PciI A'CATG_T
Cuts at: 4807 4807
Size: 5908
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5908
PmeI GTTT'AAAC
Cuts at: 3212 3212
Size: 5908
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2043 2075 3977 4009 5490 5522 2043
Size: 32 1902 32 1481 32 2429
Fragments arranged by size:
2429 1902 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3152 3152
Size: 5908
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5908
PsiI TTA'TAA
Cuts at: 3478 3478
Size: 5908
PspOMI G'GGCC_C
Cuts at: 2705 2705
Size: 5908
PstI C_TGCA'G
Cuts at: 830 3151 3260 830
Size: 2321 109 3478
Fragments arranged by size:
3478 2321 109
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5908
SacII CC_GC'GG
Cuts at: 2096 2096
Size: 5908
SalI G'TCGA_C
Cuts at: 1936 3250 1936
Size: 1314 4594
SapI GCTCTTCn'nnn_
Cuts at: 2894 2916 2894
Size: 22 5886
SbfI CC_TGCA'GG
Cuts at: 3151 3260 3151
Size: 109 5799
ScaI AGT'ACT
Cuts at: 2463 4156 5868 2463
Size: 1693 1712 2503
Fragments arranged by size:
2503 1712 1693
SexAI A'CCwGG_T
Cuts at: 2788 2788
Size: 5908
SfcI C'TryA_G
Cuts at: 826 1045 3147 3256 4391 5072 5263 826
Size: 219 2102 109 1135 681 191 1471
Fragments arranged by size:
2102 1471 1135 681 219 191 109
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5908
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5908
SmaI CCC'GGG
Cuts at: 1872 2315 3235 1872
Size: 443 920 4545
Fragments arranged by size:
4545 920 443
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4012 4913 5175 820
Size: 197 926 2069 901 262 1553
Fragments arranged by size:
2069 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3699 493
Size: 3206 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5908
SphI G_CATG'C
Cuts at: 1132 3266 1132
Size: 2134 3774
SspI AAT'ATT
Cuts at: 5 52 3832 5
Size: 47 3780 2081
Fragments arranged by size:
3780 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5908
StyI C'CwwG_G
Cuts at: 513 1065 2004 3067 3099 3347 513
Size: 552 939 1063 32 248 3074
Fragments arranged by size:
3074 1063 939 552 248 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3935 3952 4105 4290 4735 325
Size: 1255 2355 17 153 185 445 1498
Fragments arranged by size:
2355 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2461 3007 4154
Size: 274 80 33 51 1443 484 546 1147
Cuts at: 4154 5866 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1147 546 484 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5868 5900 5868
Size: 32 5876
Tth111I GACn'n_nGTC
Cuts at: 1316 2564 1316
Size: 1248 4660
XbaI T'CTAG_A
Cuts at: 3244 3244
Size: 5908
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5908
XmaI C'CCGG_G
Cuts at: 1870 2313 3233 1870
Size: 443 920 4545
Fragments arranged by size:
4545 920 443
XmnI GAAnn'nnTTC
Cuts at: 4037 4037
Size: 5908
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2341 3716 276
Size: 53 83 186 936 807 1375 2468
Fragments arranged by size:
2468 1375 936 807 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbeI BbvCI BfrBI Bpu10I
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PspXI PsrI PvuII RsrII SacI SanDI SfiI
SfoI SrfI SwaI XcmI