Restriction map for
pFN21AB4641
(Circular) (Six-base) MAPSORT of: pFN21AB4641.seq Check: 3368 from: 1 to: 7612
pFN21AB4641 7612 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5422 278
Size: 53 83 186 936 3886 2468
Fragments arranged by size:
3886 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4955 1937
Size: 3018 4594
Acc65I G'GTAC_C
Cuts at: 4933 4933
Size: 7612
AclI AA'CG_TT
Cuts at: 1563 3487 5739 6112 1563
Size: 1924 2252 373 3063
Fragments arranged by size:
3063 2252 1924 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3527 4278 5675 7059 7327 1632
Size: 1895 751 1397 1384 268 1917
Fragments arranged by size:
1917 1895 1397 1384 751 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7415
AflIII A'CryG_T
Cuts at: 1203 3006 3197 4909 6511 7488 1203
Size: 1803 191 1712 1602 977 1327
Fragments arranged by size:
1803 1712 1602 1327 977 191
AgeI A'CCGG_T
Cuts at: 6443 6443
Size: 7612
AhdI GACnn_n'nnGTC
Cuts at: 6341 6341
Size: 7612
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7612
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7612
AlwNI CAG_nnn'CTG
Cuts at: 4008 5355 6927 4008
Size: 1347 1572 4693
Fragments arranged by size:
4693 1572 1347
ApaLI G'TGCA_C
Cuts at: 5669 6825 5669
Size: 1156 6456
ApoI r'AATT_y
Cuts at: 1493 2555 2708 2768 2961 3341 3739 3970 4070
Size: 1062 153 60 193 380 398 231 100
Cuts at: 4070 4683 4726 4921 5147 5299 7408 1493
Size: 613 43 195 226 152 2109 1697
Fragments arranged by size:
2109 1697 1062 613 398 380 231 226
195 193 153 152 100 60 43
AseI AT'TA_AT
Cuts at: 160 1032 6166 160
Size: 872 5134 1606
Fragments arranged by size:
5134 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7612
AvaI C'yCGr_G
Cuts at: 1870 1943 2063 4937 1870
Size: 73 120 2874 4545
Fragments arranged by size:
4545 2874 120 73
AvrII C'CTAG_G
Cuts at: 4771 4771
Size: 7612
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4011 4044 4011
Size: 33 7579
BamHI G'GATC_C
Cuts at: 4942 5309 4942
Size: 367 7245
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3612 4775 4933 6388 618
Size: 325 217 627 1825 1163 158 1455 1842
Fragments arranged by size:
1842 1825 1455 1163 627 325 217 158
BanII G_rGCy'C
Cuts at: 1391 2118 4320 4931 1391
Size: 727 2202 611 4072
Fragments arranged by size:
4072 2202 727 611
BbeI G_GCGC'C
Cuts at: 3616 3616
Size: 7612
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3126 4892 928
Size: 971 1227 1766 3648
Fragments arranged by size:
3648 1766 1227 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4583 4617 5803 5837 1903
Size: 34 2646 34 1186 34 3678
Fragments arranged by size:
3678 2646 1186 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4012 5504 6714 4012
Size: 1492 1210 4910
Fragments arranged by size:
4910 1492 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7612
BfrBI ATG'CAT
Cuts at: 3242 3780 4696 4700 3242
Size: 538 916 4 6154
Fragments arranged by size:
6154 916 538 4
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6223 136
Size: 107 122 71 631 605 4551 1525
Fragments arranged by size:
4551 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3169 7607 3169
Size: 4438 3174
BlpI GC'TnA_GC
Cuts at: 5029 5029
Size: 7612
Bme1580I G_kGCm'C
Cuts at: 1790 5673 6829 1790
Size: 3883 1156 2573
Fragments arranged by size:
3883 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7612
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4203 5343 6301
Size: 416 53 83 399 24 2786 1140 958
Cuts at: 6301 442
Size: 1753
Fragments arranged by size:
2786 1753 1140 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3623 1056
Size: 2567 5045
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2045 2132 2432 2741 3468 6272 1239
Size: 806 87 300 309 727 2804 2579
Fragments arranged by size:
2804 2579 806 727 309 300 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2535 3255 4371 5737 6602 6900 2535
Size: 720 1116 1366 865 298 3247
Fragments arranged by size:
3247 1366 1116 865 720 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2084 6275 882
Size: 617 585 4191 2219
Fragments arranged by size:
4191 2219 617 585
BsaAI yAC'GTr
Cuts at: 493 1204 3007 3076 3200 5403 493
Size: 711 1803 69 124 2203 2702
Fragments arranged by size:
2702 2203 1803 711 124 69
BsaBI GATnn'nnATC
Cuts at: 4527 7606 4527
Size: 3079 4533
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3613 4785 5419 5801
Size: 53 83 186 936 2080 1172 634 382
Cuts at: 5801 6436 275
Size: 635 1451
Fragments arranged by size:
2080 1451 1172 936 635 634 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4447 6045 6443 6717 6864 7275 4447
Size: 1598 398 274 147 411 4784
Fragments arranged by size:
4784 1598 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7387
Fragments arranged by size:
7387 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2081 2263 3054 3362 3743 4493 1186
Size: 895 182 791 308 381 750 4305
Fragments arranged by size:
4305 895 791 750 381 308 182
BseYI C'CCAG_C
Cuts at: 2043 2389 6815 7311 2043
Size: 346 4426 496 2344
Fragments arranged by size:
4426 2344 496 346
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2155 1163
Size: 573 419 6620
Fragments arranged by size:
6620 573 419
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5062 5823 6851 1540
Size: 275 186 3061 761 1028 2301
Fragments arranged by size:
3061 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2085 4126 5673 5758 6829 2085
Size: 2041 1547 85 1071 2868
Fragments arranged by size:
2868 2041 1547 1071 85
BsmI GAATG_Cn'
Cuts at: 2121 2842 4340 5123 5216 2121
Size: 721 1498 783 93 4517
Fragments arranged by size:
4517 1498 783 721 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2045 2388 2045
Size: 343 7269
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2085 2118 4126 4320 4931 5673 5758
Size: 399 295 33 2008 194 611 742 85
Cuts at: 5758 6829 1391
Size: 1071 2174
Fragments arranged by size:
2174 2008 1071 742 611 399 295 194
85 33
BspEI T'CCGG_A
Cuts at: 4447 7275 4447
Size: 2828 4784
BspHI T'CATG_A
Cuts at: 5501 7231 5501
Size: 1730 5882
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3451 4380 4967 844
Size: 2607 929 587 3489
Fragments arranged by size:
3489 2607 929 587
BsrBI CCG'CTC
Cuts at: 1394 1823 5499 1394
Size: 429 3676 3507
Fragments arranged by size:
3676 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3226 4543 4851 6107 6281 65
Size: 1183 1978 1317 308 1256 174 1396
Fragments arranged by size:
1978 1396 1317 1256 1183 308 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6256 6443 1700
Size: 110 4446 187 2869
Fragments arranged by size:
4446 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7612
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7612
BssSI C'ACGA_G
Cuts at: 4264 5672 6684 4264
Size: 1408 1012 5192
Fragments arranged by size:
5192 1408 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2893 4838 794
Size: 2099 1945 3568
Fragments arranged by size:
3568 2099 1945
BstBI TT'CG_AA
Cuts at: 6416 6416
Size: 7612
BstEII G'GTnAC_C
Cuts at: 1184 3438 1184
Size: 2254 5358
BstYI r'GATC_y
Cuts at: 1972 3082 3169 4516 4942 5309 5694 5711 7163
Size: 1110 87 1347 426 367 385 17 1452
Cuts at: 7163 7249 7261 7607 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1347 1110 426 385 367 346
87 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2012 4138 513
Size: 552 939 8 2126 3987
Fragments arranged by size:
3987 2126 939 552 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3052 3588 487
Size: 683 56 1826 536 4511
Fragments arranged by size:
4511 1826 683 536 56
BtsI GCAGTG_nn'
Cuts at: 5128 5922 5942 5128
Size: 794 20 6798
Fragments arranged by size:
6798 794 20
ClaI AT'CG_AT
Cuts at: 1554 2229 1554
Size: 675 6937
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2662 2697 529
Size: 35 2098 35 5444
Fragments arranged by size:
5444 2098 35 35
DraI TTT'AAA
Cuts at: 4888 4916 4888
Size: 28 7584
DraIII CAC_nnn'GTG
Cuts at: 3770 3770
Size: 7612
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6619 809
Size: 507 5303 1802
Fragments arranged by size:
5303 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3058 4010 5059
Size: 54 1425 50 275 18 1228 952 1049
Cuts at: 5059 5948 7577 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1228 1049 952 889 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5059 1537
Size: 275 3247 4090
Fragments arranged by size:
4090 3247 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3408 3720 4053 5542 1353
Size: 2055 312 333 1489 3423
Fragments arranged by size:
3423 2055 1489 333 312
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2063 4081 6199 6573 6719 387
Size: 1064 138 474 2018 2118 374 146 1280
Fragments arranged by size:
2118 2018 1280 1064 474 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2132 2432 2741 3468 3527 4278
Size: 393 413 87 300 309 727 59 751
Cuts at: 4278 5675 6272 7059 7327 1239
Size: 1397 597 787 268 1524
Fragments arranged by size:
1524 1397 787 751 727 597 413 393
309 300 268 87 59
EcoNI CCTnn'n_nnAGG
Cuts at: 3467 3467
Size: 7612
EcoO109I rG'GnC_Cy
Cuts at: 3000 4144 4369 3000
Size: 1144 225 6243
Fragments arranged by size:
6243 1144 225
EcoRI G'AATT_C
Cuts at: 4921 5299 4921
Size: 378 7234
EcoRV GAT'ATC
Cuts at: 4563 4563
Size: 7612
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3616 3616
Size: 7612
FspI TGC'GCA
Cuts at: 2621 6118 2621
Size: 3497 4115
HaeII r_GCGC'y
Cuts at: 1678 2044 3616 6759 1678
Size: 366 1572 3143 2531
Fragments arranged by size:
3143 2531 1572 366
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2057 2089 2385 2417 2579
Size: 32 135 32 485 32 296 32 162
Cuts at: 2579 2611 2747 2779 3074 3106 3984 4016 4552
Size: 32 136 32 295 32 878 32 536
Cuts at: 4552 4584 6253 6285 6327 6359 1373
Size: 32 1669 32 42 32 2626
Fragments arranged by size:
2626 1669 878 536 485 296 295 162
136 135 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2305 2751 4956 5202 669
Size: 988 281 367 446 2205 246 3079
Fragments arranged by size:
3079 2205 988 446 367 281 246
HindIII A'AGCT_T
Cuts at: 748 2696 748
Size: 1948 5664
HpaI GTT'AAC
Cuts at: 2751 5202 2751
Size: 2451 5161
KasI G'GCGC_C
Cuts at: 3612 3612
Size: 7612
KpnI G_GTAC'C
Cuts at: 4937 4937
Size: 7612
MfeI C'AATT_G
Cuts at: 4734 4827 5211 4734
Size: 93 384 7135
Fragments arranged by size:
7135 384 93
MluI A'CGCG_T
Cuts at: 7488 7488
Size: 7612
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6726 6910 1305
Size: 5421 184 2007
Fragments arranged by size:
5421 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3060 4012 10
Size: 54 1425 343 1228 952 3610
Fragments arranged by size:
3610 1425 1228 952 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2994 5570 5929 6088 518
Size: 648 1828 2576 359 159 2042
Fragments arranged by size:
2576 2042 1828 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2014 2023 3634 5028 5705 6853 7098 1606
Size: 408 9 1611 1394 677 1148 245 2120
Fragments arranged by size:
2120 1611 1394 1148 677 408 245 9
NarI GG'CG_CC
Cuts at: 3613 3613
Size: 7612
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6121
Fragments arranged by size:
6121 939 552
NdeI CA'TA_TG
Cuts at: 387 4202 387
Size: 3815 3797
NheI G'CTAG_C
Cuts at: 1052 3619 1052
Size: 2567 5045
NotI GC'GGCC_GC
Cuts at: 5059 5059
Size: 7612
NsiI A_TGCA'T
Cuts at: 3244 3782 4698 4702 3244
Size: 538 916 4 6154
Fragments arranged by size:
6154 916 538 4
NspI r_CATG'y
Cuts at: 1132 1229 2389 4700 4913 4970 6515 1132
Size: 97 1160 2311 213 57 1545 2229
Fragments arranged by size:
2311 2229 1545 1160 213 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7612
PciI A'CATG_T
Cuts at: 4909 6511 4909
Size: 1602 6010
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3092 4215 1108
Size: 1984 1123 4505
Fragments arranged by size:
4505 1984 1123
PfoI T'CCnGG_A
Cuts at: 3978 3978
Size: 7612
PmeI GTTT'AAAC
Cuts at: 4916 4916
Size: 7612
PmlI CAC'GTG
Cuts at: 3076 3076
Size: 7612
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5681 5713 7194 7226 5681
Size: 32 1481 32 6067
Fragments arranged by size:
6067 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3000 4369 3000
Size: 1369 6243
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7612
PsiI TTA'TAA
Cuts at: 3531 5182 3531
Size: 1651 5961
PstI C_TGCA'G
Cuts at: 830 2022 3834 4964 830
Size: 1192 1812 1130 3478
Fragments arranged by size:
3478 1812 1192 1130
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7612
PvuII CAG'CTG
Cuts at: 2023 3634 2023
Size: 1611 6001
SacII CC_GC'GG
Cuts at: 2015 2015
Size: 7612
SalI G'TCGA_C
Cuts at: 1936 4954 1936
Size: 3018 4594
SbfI CC_TGCA'GG
Cuts at: 4964 4964
Size: 7612
ScaI AGT'ACT
Cuts at: 2453 3839 5860 7572 2453
Size: 1386 2021 1712 2493
Fragments arranged by size:
2493 2021 1712 1386
SfcI C'TryA_G
Cuts at: 826 1045 2018 3830 4960 6095 6776 6967 826
Size: 219 973 1812 1130 1135 681 191 1471
Fragments arranged by size:
1812 1471 1135 1130 973 681 219 191
SfoI GGC'GCC
Cuts at: 3614 3614
Size: 7612
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7612
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7612
SmaI CCC'GGG
Cuts at: 1872 4939 1872
Size: 3067 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2550 3270 4386 5716 6617 6879
Size: 197 926 607 720 1116 1330 901 262
Cuts at: 6879 820
Size: 1553
Fragments arranged by size:
1553 1330 1116 926 901 720 607 262
197
SnaBI TAC'GTA
Cuts at: 493 5403 493
Size: 4910 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7612
SphI G_CATG'C
Cuts at: 1132 4700 4970 1132
Size: 3568 270 3774
Fragments arranged by size:
3774 3568 270
SspI AAT'ATT
Cuts at: 5 52 2626 5536 5
Size: 47 2574 2910 2081
Fragments arranged by size:
2910 2574 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7612
StyI C'CwwG_G
Cuts at: 513 1065 2004 2284 2517 2734 4771 4780 5051
Size: 552 939 280 233 217 2037 9 271
Cuts at: 5051 513
Size: 3074
Fragments arranged by size:
3074 2037 939 552 280 271 233 217
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3574 5639 5656 5809 5994 6439 325
Size: 1255 1994 2065 17 153 185 445 1498
Fragments arranged by size:
2065 1994 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2451 3837 5858
Size: 274 80 33 51 1443 474 1386 2021
Cuts at: 5858 7570 96
Size: 1712 138
Fragments arranged by size:
2021 1712 1443 1386 474 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3570 3602 7572 7604 3570
Size: 32 3970 32 3578
Fragments arranged by size:
3970 3578 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3996 1316
Size: 2680 4932
XbaI T'CTAG_A
Cuts at: 4948 4948
Size: 7612
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7612
XmaI C'CCGG_G
Cuts at: 1870 4937 1870
Size: 3067 4545
XmnI GAAnn'nnTTC
Cuts at: 3753 5741 3753
Size: 1988 5624
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5420 276
Size: 53 83 186 936 3886 2468
Fragments arranged by size:
3886 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BbvCI BplI Bpu10I
BsiWI BstXI BstZ17I Bsu36I EcoICRI FseI FspAI NaeI
NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacI
SanDI SapI SexAI SfiI SrfI SwaI XcmI