Restriction map for
pFN21AB4640
(Circular) (Six-base) MAPSORT of: pFN21AB4640.seq Check: 1786 from: 1 to: 6547
pFN21AB4640 6547 bp
With 182 enzymes: SgfI *
October 20, 2009 10:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4357 278
Size: 53 83 186 936 2821 2468
Fragments arranged by size:
2821 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3890 1937
Size: 1953 4594
Acc65I G'GTAC_C
Cuts at: 3868 3868
Size: 6547
AclI AA'CG_TT
Cuts at: 1563 4674 5047 1563
Size: 3111 373 3063
Fragments arranged by size:
3111 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2421 3122 3320 3567 4610 5994 6262 1632
Size: 789 701 198 247 1043 1384 268 1917
Fragments arranged by size:
1917 1384 1043 789 701 268 247 198
AflII C'TTAA_G
Cuts at: 820 1017 2393 820
Size: 197 1376 4974
Fragments arranged by size:
4974 1376 197
AflIII A'CryG_T
Cuts at: 1203 5446 6423 1203
Size: 4243 977 1327
Fragments arranged by size:
4243 1327 977
AgeI A'CCGG_T
Cuts at: 5378 5378
Size: 6547
AhdI GACnn_n'nnGTC
Cuts at: 5276 5276
Size: 6547
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6547
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6547
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3726 3758 3726
Size: 32 6515
AlwNI CAG_nnn'CTG
Cuts at: 2733 4290 5862 2733
Size: 1557 1572 3418
Fragments arranged by size:
3418 1572 1557
ApaI G_GGCC'C
Cuts at: 2535 2535
Size: 6547
ApaLI G'TGCA_C
Cuts at: 4604 5760 4604
Size: 1156 5391
ApoI r'AATT_y
Cuts at: 1493 2487 3856 4082 4234 6343 1493
Size: 994 1369 226 152 2109 1697
Fragments arranged by size:
2109 1697 1369 994 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5101 160
Size: 872 4069 1606
Fragments arranged by size:
4069 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6547
AvaI C'yCGr_G
Cuts at: 1870 1943 3872 1870
Size: 73 1929 4545
Fragments arranged by size:
4545 1929 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3028 3061 3028
Size: 33 6514
BamHI G'GATC_C
Cuts at: 3877 4244 3877
Size: 367 6180
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3868 5323 618
Size: 325 217 627 2081 1455 1842
Fragments arranged by size:
2081 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2535 3231 3866 1391
Size: 1144 696 635 4072
Fragments arranged by size:
4072 1144 696 635
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3555 3678 928
Size: 971 1656 123 3797
Fragments arranged by size:
3797 1656 971 123
BbvCI CC'TCA_GC
Cuts at: 3650 3650
Size: 6547
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4738 4772 1903
Size: 34 2801 34 3678
Fragments arranged by size:
3678 2801 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4439 5649 4439
Size: 1210 5337
BclI T'GATC_A
Cuts at: 1548 2887 1548
Size: 1339 5208
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5158 136
Size: 107 122 71 631 605 3486 1525
Fragments arranged by size:
3486 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2341 6542 2341
Size: 4201 2346
BlpI GC'TnA_GC
Cuts at: 2271 3964 2271
Size: 1693 4854
Bme1580I G_kGCm'C
Cuts at: 1790 2535 4608 5764 1790
Size: 745 2073 1156 2573
Fragments arranged by size:
2573 2073 1156 745
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6547
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2387 3628 4278
Size: 416 53 83 399 24 970 1241 650
Cuts at: 4278 5236 442
Size: 958 1753
Fragments arranged by size:
1753 1241 970 958 650 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6547
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2020 2342 2390 2561 3687 5207 1239
Size: 781 322 48 171 1126 1520 2579
Fragments arranged by size:
2579 1520 1126 781 322 171 48
Bpu10I CC'TnA_GC
Cuts at: 2493 3348 3650 2493
Size: 855 302 5390
Fragments arranged by size:
5390 855 302
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2774 4672 5537 5835 2774
Size: 1898 865 298 3486
Fragments arranged by size:
3486 1898 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2475 5210 882
Size: 617 976 2735 2219
Fragments arranged by size:
2735 2219 976 617
BsaAI yAC'GTr
Cuts at: 493 1204 4338 493
Size: 711 3134 2702
Fragments arranged by size:
3134 2702 711
BsaBI GATnn'nnATC
Cuts at: 6541 6541
Size: 6547
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4354 4736 5371 275
Size: 53 83 186 936 2821 382 635 1451
Fragments arranged by size:
2821 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2585 4980 5378 5652 5799 6210 2585
Size: 2395 398 274 147 411 2922
Fragments arranged by size:
2922 2395 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3269 3299 1178
Size: 30 165 30 1866 30 4426
Fragments arranged by size:
4426 1866 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2264 2777 3221 3825 1186
Size: 1078 513 444 604 3908
Fragments arranged by size:
3908 1078 604 513 444
BseYI C'CCAG_C
Cuts at: 2663 3149 5750 6246 2663
Size: 486 2601 496 2964
Fragments arranged by size:
2964 2601 496 486
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3217 3235 1163
Size: 573 1481 18 4475
Fragments arranged by size:
4475 1481 573 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3997 4758 5786 1540
Size: 275 186 1996 761 1028 2301
Fragments arranged by size:
2301 1996 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2499 4608 4693 5764 2499
Size: 2109 85 1071 3282
Fragments arranged by size:
3282 2109 1071 85
BsmI GAATG_Cn'
Cuts at: 4058 4151 4058
Size: 93 6454
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2499 2535 3231 3866 4608 4693 5764
Size: 399 709 36 696 635 742 85 1071
Cuts at: 5764 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 709 696 635 399 85
36
BspEI T'CCGG_A
Cuts at: 6210 6210
Size: 6547
BspHI T'CATG_A
Cuts at: 4436 6166 4436
Size: 1730 4817
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2641 3902 844
Size: 1797 1261 3489
Fragments arranged by size:
3489 1797 1261
BsrBI CCG'CTC
Cuts at: 1394 1823 4434 1394
Size: 429 2611 3507
Fragments arranged by size:
3507 2611 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3438 5042 5216 65
Size: 1183 2190 1604 174 1396
Fragments arranged by size:
2190 1604 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5191 5378 1700
Size: 110 3381 187 2869
Fragments arranged by size:
3381 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6547
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6547
BssSI C'ACGA_G
Cuts at: 4607 5619 4607
Size: 1012 5535
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6547
BstBI TT'CG_AA
Cuts at: 3688 5351 3688
Size: 1663 4884
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6547
BstXI CCAn_nnnn'nTGG
Cuts at: 2857 3076 2857
Size: 219 6328
BstYI r'GATC_y
Cuts at: 1972 2341 3877 4244 4629 4646 6098 6184 6196
Size: 369 1536 367 385 17 1452 86 12
Cuts at: 6196 6542 1972
Size: 346 1977
Fragments arranged by size:
1977 1536 1452 385 369 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5995
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5808
Fragments arranged by size:
5808 683 56
BtsI GCAGTG_nn'
Cuts at: 4063 4857 4877 4063
Size: 794 20 5733
Fragments arranged by size:
5733 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6547
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6512
DraI TTT'AAA
Cuts at: 3574 3851 3574
Size: 277 6270
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5554 809
Size: 507 4238 1802
Fragments arranged by size:
4238 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3994 4883 6512
Size: 54 1425 50 275 18 2164 889 1629
Cuts at: 6512 8
Size: 43
Fragments arranged by size:
2164 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3994 1537
Size: 275 2182 4090
Fragments arranged by size:
4090 2182 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2540 4477 1353
Size: 1187 1937 3423
Fragments arranged by size:
3423 1937 1187
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5134 5508 5654 387
Size: 1064 138 3545 374 146 1280
Fragments arranged by size:
3545 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2020 2342 2390 2421 2561 3122 3320
Size: 393 388 322 48 31 140 561 198
Cuts at: 3320 3567 3687 4610 5207 5994 6262 1239
Size: 247 120 923 597 787 268 1524
Fragments arranged by size:
1524 923 787 597 561 393 388 322
268 247 198 140 120 48 31
EcoO109I rG'GnC_Cy
Cuts at: 2532 3190 3504 3622 2532
Size: 658 314 118 5457
Fragments arranged by size:
5457 658 314 118
EcoRI G'AATT_C
Cuts at: 3856 4234 3856
Size: 378 6169
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2719 3728 2719
Size: 1009 5538
FspI TGC'GCA
Cuts at: 5053 5053
Size: 6547
HaeII r_GCGC'y
Cuts at: 1678 5694 1678
Size: 4016 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2541 2561 2573 2593 5188
Size: 32 135 32 969 20 12 20 2595
Cuts at: 5188 5220 5262 5294 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2595 969 135 42 32 32 32
32 20 20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3891 4137 669
Size: 988 281 1953 246 3079
Fragments arranged by size:
3079 1953 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3238 748
Size: 2490 4057
HpaI GTT'AAC
Cuts at: 4137 4137
Size: 6547
KpnI G_GTAC'C
Cuts at: 3872 3872
Size: 6547
MfeI C'AATT_G
Cuts at: 2302 4146 2302
Size: 1844 4703
MluI A'CGCG_T
Cuts at: 6423 6423
Size: 6547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2848 3468 5661 5845 1305
Size: 1543 620 2193 184 2007
Fragments arranged by size:
2193 2007 1543 620 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4725
Fragments arranged by size:
4725 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2855 4505 4864 5023 518
Size: 648 1689 1650 359 159 2042
Fragments arranged by size:
2042 1689 1650 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3614 3963 4640 5788 6033 1606
Size: 2008 349 677 1148 245 2120
Fragments arranged by size:
2120 2008 1148 677 349 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5995
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6547
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6547
NotI GC'GGCC_GC
Cuts at: 3994 3994
Size: 6547
NspI r_CATG'y
Cuts at: 1132 1229 3905 5450 1132
Size: 97 2676 1545 2229
Fragments arranged by size:
2676 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6547
PciI A'CATG_T
Cuts at: 5446 5446
Size: 6547
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6547
PfoI T'CCnGG_A
Cuts at: 2538 2538
Size: 6547
PmeI GTTT'AAAC
Cuts at: 3851 3851
Size: 6547
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4616 4648 6129 6161 4616
Size: 32 1481 32 5002
Fragments arranged by size:
5002 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3190 3622 3190
Size: 432 6115
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6547
PsiI TTA'TAA
Cuts at: 4117 4117
Size: 6547
PspOMI G'GGCC_C
Cuts at: 2531 2531
Size: 6547
PstI C_TGCA'G
Cuts at: 830 3772 3899 830
Size: 2942 127 3478
Fragments arranged by size:
3478 2942 127
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6547
PvuII CAG'CTG
Cuts at: 3614 3614
Size: 6547
SalI G'TCGA_C
Cuts at: 1936 3889 1936
Size: 1953 4594
SbfI CC_TGCA'GG
Cuts at: 3899 3899
Size: 6547
ScaI AGT'ACT
Cuts at: 4795 6507 4795
Size: 1712 4835
SfcI C'TryA_G
Cuts at: 826 1045 3725 3768 3839 3895 5030 5711 5902
Size: 219 2680 43 71 56 1135 681 191
Cuts at: 5902 826
Size: 1471
Fragments arranged by size:
2680 1471 1135 681 219 191 71 56
43
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6547
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6547
SmaI CCC'GGG
Cuts at: 1872 3874 1872
Size: 2002 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2393 2789 4651 5552 5814 820
Size: 197 926 450 396 1862 901 262 1553
Fragments arranged by size:
1862 1553 926 901 450 396 262 197
SnaBI TAC'GTA
Cuts at: 493 4338 493
Size: 3845 2702
SpeI A'CTAG_T
Cuts at: 152 2837 152
Size: 2685 3862
SphI G_CATG'C
Cuts at: 1132 3905 1132
Size: 2773 3774
SspI AAT'ATT
Cuts at: 5 52 2527 4471 5
Size: 47 2475 1944 2081
Fragments arranged by size:
2475 2081 1944 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6547
StyI C'CwwG_G
Cuts at: 513 1065 2620 2703 3528 3986 513
Size: 552 1555 83 825 458 3074
Fragments arranged by size:
3074 1555 825 552 458 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4574 4591 4744 4929 5374 325
Size: 1255 2994 17 153 185 445 1498
Fragments arranged by size:
2994 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4793 6505 96
Size: 274 80 33 51 1443 2816 1712 138
Fragments arranged by size:
2816 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6507 6539 6507
Size: 32 6515
Tth111I GACn'n_nGTC
Cuts at: 1316 3620 1316
Size: 2304 4243
XbaI T'CTAG_A
Cuts at: 3443 3883 3443
Size: 440 6107
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2024 3151 3662 2024
Size: 1127 511 4909
Fragments arranged by size:
4909 1127 511
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6547
XmaI C'CCGG_G
Cuts at: 1870 3872 1870
Size: 2002 4545
XmnI GAAnn'nnTTC
Cuts at: 2012 4676 2012
Size: 2664 3883
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4355 276
Size: 53 83 186 936 2821 2468
Fragments arranged by size:
2821 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI