Restriction map for
pFN21AB4636
(Circular) (Six-base) MAPSORT of: pFN21AB4636.seq Check: 136 from: 1 to: 8083
pFN21AB4636 8083 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2776 3057 3448 3997 2776
Size: 281 391 549 6862
Fragments arranged by size:
6862 549 391 281
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5893 278
Size: 53 83 186 936 4357 2468
Fragments arranged by size:
4357 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5426 1937
Size: 3489 4594
Acc65I G'GTAC_C
Cuts at: 3581 5404 3581
Size: 1823 6260
AclI AA'CG_TT
Cuts at: 1563 6210 6583 1563
Size: 4647 373 3063
Fragments arranged by size:
4647 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2204 3785 4736 4980 6146 7530 7798 1632
Size: 572 1581 951 244 1166 1384 268 1917
Fragments arranged by size:
1917 1581 1384 1166 951 572 268 244
AfeI AGC'GCT
Cuts at: 2873 5345 2873
Size: 2472 5611
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7886
AflIII A'CryG_T
Cuts at: 1203 6982 7959 1203
Size: 5779 977 1327
Fragments arranged by size:
5779 1327 977
AgeI A'CCGG_T
Cuts at: 6914 6914
Size: 8083
AhdI GACnn_n'nnGTC
Cuts at: 6812 6812
Size: 8083
AleI CACnn'nnGTG
Cuts at: 1166 3333 1166
Size: 2167 5916
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2160 5162 1184
Size: 976 3002 4105
Fragments arranged by size:
4105 3002 976
AlwNI CAG_nnn'CTG
Cuts at: 2842 3554 3689 3817 3851 4913 5183 5826 7398
Size: 712 135 128 34 1062 270 643 1572
Cuts at: 7398 2842
Size: 3527
Fragments arranged by size:
3527 1572 1062 712 643 270 135 128
34
ApaI G_GGCC'C
Cuts at: 2110 2571 2762 3546 4228 4576 5162 2110
Size: 461 191 784 682 348 586 5031
Fragments arranged by size:
5031 784 682 586 461 348 191
ApaLI G'TGCA_C
Cuts at: 6140 7296 6140
Size: 1156 6927
ApoI r'AATT_y
Cuts at: 1493 4505 4680 5392 5618 5770 7879 1493
Size: 3012 175 712 226 152 2109 1697
Fragments arranged by size:
3012 2109 1697 712 226 175 152
AseI AT'TA_AT
Cuts at: 160 1032 6637 160
Size: 872 5605 1606
Fragments arranged by size:
5605 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8083
AvaI C'yCGr_G
Cuts at: 1870 1943 2355 2490 2676 3307 3486 4140 4941
Size: 73 412 135 186 631 179 654 801
Cuts at: 4941 4974 5231 5408 1870
Size: 33 257 177 4545
Fragments arranged by size:
4545 801 654 631 412 257 186 179
177 135 73 33
BamHI G'GATC_C
Cuts at: 2339 4190 5413 5780 2339
Size: 1851 1223 367 4642
Fragments arranged by size:
4642 1851 1223 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3190 3460 3569 3581 3713
Size: 325 217 627 1403 270 109 12 132
Cuts at: 3713 5257 5404 6859 618
Size: 1544 147 1455 1842
Fragments arranged by size:
1842 1544 1455 1403 627 325 270 217
147 132 109 12
BanII G_rGCy'C
Cuts at: 1391 2110 2269 2490 2571 2639 2685 2736 2762
Size: 719 159 221 81 68 46 51 26
Cuts at: 2762 3268 3546 4130 4141 4228 4383 4576 4973
Size: 506 278 584 11 87 155 193 397
Cuts at: 4973 5162 5215 5231 5402 1391
Size: 189 53 16 171 4072
Fragments arranged by size:
4072 719 584 506 397 278 221 193
189 171 159 155 87 81 68 53
51 46 26 16 11
BbeI G_GCGC'C
Cuts at: 3573 3573
Size: 8083
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2075 3225 5328 928
Size: 971 176 1150 2103 3683
Fragments arranged by size:
3683 2103 1150 971 176
BbvCI CC'TCA_GC
Cuts at: 2320 3253 3258 4887 2320
Size: 933 5 1629 5516
Fragments arranged by size:
5516 1629 933 5
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6274 6308 1903
Size: 34 4337 34 3678
Fragments arranged by size:
4337 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5975 7185 5975
Size: 1210 6873
BclI T'GATC_A
Cuts at: 1548 2520 2952 4419 1548
Size: 972 432 1467 5212
Fragments arranged by size:
5212 1467 972 432
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3596 3841 5363
Size: 107 122 71 631 605 1924 245 1522
Cuts at: 5363 6694 136
Size: 1331 1525
Fragments arranged by size:
1924 1525 1522 1331 631 605 245 122
107 71
BglII A'GATC_T
Cuts at: 8078 8078
Size: 8083
BlpI GC'TnA_GC
Cuts at: 2511 4112 5500 2511
Size: 1601 1388 5094
Fragments arranged by size:
5094 1601 1388
Bme1580I G_kGCm'C
Cuts at: 1790 2110 2571 2716 2762 3546 4228 4576 5162
Size: 320 461 145 46 784 682 348 586
Cuts at: 5162 6144 7300 1790
Size: 982 1156 2573
Fragments arranged by size:
2573 1156 982 784 682 586 461 348
320 145 46
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8083
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3542 4383 5814
Size: 416 53 83 399 24 2125 841 1431
Cuts at: 5814 6772 442
Size: 958 1753
Fragments arranged by size:
2125 1753 1431 958 841 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8083
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4987 4987
Size: 8083
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2810 2840 2864 3080 3558 3873 4071 4233
Size: 1571 30 24 216 478 315 198 162
Cuts at: 4233 4313 4604 4784 5273 6743 1239
Size: 80 291 180 489 1470 2579
Fragments arranged by size:
2579 1571 1470 489 478 315 291 216
198 180 162 80 30 24
Bpu10I CC'TnA_GC
Cuts at: 2320 3253 3258 3654 4881 4887 5199 5240 2320
Size: 933 5 396 1227 6 312 41 5163
Fragments arranged by size:
5163 1227 933 396 312 41 6 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2653 4085 6208 7073 7371 2653
Size: 1432 2123 865 298 3365
Fragments arranged by size:
3365 2123 1432 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6746 882
Size: 617 5247 2219
Fragments arranged by size:
5247 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5874 493
Size: 711 4670 2702
Fragments arranged by size:
4670 2702 711
BsaBI GATnn'nnATC
Cuts at: 8077 8077
Size: 8083
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3570 5890 6272 6907
Size: 53 83 186 936 2037 2320 382 635
Cuts at: 6907 275
Size: 1451
Fragments arranged by size:
2320 2037 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2382 3318 4078 4398 4491 5292 6516 6914 7188
Size: 936 760 320 93 801 1224 398 274
Cuts at: 7188 7335 7746 2382
Size: 147 411 2719
Fragments arranged by size:
2719 1224 936 801 760 411 398 320
274 147 93
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3940 3970 4311 4341 1178
Size: 30 165 30 2537 30 341 30 4920
Fragments arranged by size:
4920 2537 341 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2259 2262 2577 2720 2951 3501 3963
Size: 868 205 3 315 143 231 550 462
Cuts at: 3963 4856 1186
Size: 893 4413
Fragments arranged by size:
4413 893 868 550 462 315 231 205
143 3
BseYI C'CCAG_C
Cuts at: 2716 2746 2752 2983 3762 4123 4335 4827 4906
Size: 30 6 231 779 361 212 492 79
Cuts at: 4906 5163 5340 5358 7286 7782 2716
Size: 257 177 18 1928 496 3017
Fragments arranged by size:
3017 1928 779 496 492 361 257 231
212 177 79 30 18 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3016 4121 4484 4613 1163
Size: 573 1280 1105 363 129 4633
Fragments arranged by size:
4633 1280 1105 573 363 129
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5533 6294 7322 1540
Size: 275 186 3532 761 1028 2301
Fragments arranged by size:
3532 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2139 2639 2736 2826 3096 3409 4113 4770 5215
Size: 500 97 90 270 313 704 657 445
Cuts at: 5215 6144 6229 7300 2139
Size: 929 85 1071 2922
Fragments arranged by size:
2922 1071 929 704 657 500 445 313
270 97 90 85
BsmI GAATG_Cn'
Cuts at: 2392 4616 4903 5594 5687 2392
Size: 2224 287 691 93 4788
Fragments arranged by size:
4788 2224 691 287 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5128 5128
Size: 8083
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2110 2139 2269 2490 2571 2639 2685
Size: 399 320 29 130 221 81 68 46
Cuts at: 2685 2716 2736 2762 2826 3096 3268 3409 3546
Size: 31 20 26 64 270 172 141 137
Cuts at: 3546 4113 4130 4141 4228 4383 4576 4770 4973
Size: 567 17 11 87 155 193 194 203
Cuts at: 4973 5162 5215 5231 5402 6144 6229 7300 1391
Size: 189 53 16 171 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 567 399 320 270 221
203 194 193 189 172 171 155 141
137 130 87 85 81 68 64 53
46 31 29 26 20 17 16 11
BspEI T'CCGG_A
Cuts at: 3318 4398 4491 7746 3318
Size: 1080 93 3255 3655
Fragments arranged by size:
3655 3255 1080 93
BspHI T'CATG_A
Cuts at: 2377 3351 5972 7702 2377
Size: 974 2621 1730 2758
Fragments arranged by size:
2758 2621 1730 974
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2776 3021 3057 3448 3834 3997 4456 4635
Size: 1932 245 36 391 386 163 459 179
Cuts at: 4635 5438 844
Size: 803 3489
Fragments arranged by size:
3489 1932 803 459 391 386 245 179
163 36
BsrBI CCG'CTC
Cuts at: 1394 1823 3612 3746 5970 1394
Size: 429 1789 134 2224 3507
Fragments arranged by size:
3507 2224 1789 429 134
BsrDI GCAATG_nn'
Cuts at: 65 1248 2139 4530 4549 6578 6752 65
Size: 1183 891 2391 19 2029 174 1396
Fragments arranged by size:
2391 2029 1396 1183 891 174 19
BsrFI r'CCGG_y
Cuts at: 1700 1810 2605 3572 6727 6914 1700
Size: 110 795 967 3155 187 2869
Fragments arranged by size:
3155 2869 967 795 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8083
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8083
BssSI C'ACGA_G
Cuts at: 2924 4695 6143 7155 2924
Size: 1771 1448 1012 3852
Fragments arranged by size:
3852 1771 1448 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4009 4108 4546 794
Size: 3215 99 438 4331
Fragments arranged by size:
4331 3215 438 99
BstBI TT'CG_AA
Cuts at: 6887 6887
Size: 8083
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8083
BstXI CCAn_nnnn'nTGG
Cuts at: 3088 3886 4738 3088
Size: 798 852 6433
Fragments arranged by size:
6433 852 798
BstYI r'GATC_y
Cuts at: 1972 2339 4190 5413 5780 6165 6182 7634 7720
Size: 367 1851 1223 367 385 17 1452 86
Cuts at: 7720 7732 8078 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1851 1452 1223 385 367 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4839 4962 4839
Size: 123 7960
BtgI C'CryG_G
Cuts at: 513 1065 2004 2685 2904 3087 3222 3345 3789
Size: 552 939 681 219 183 135 123 444
Cuts at: 3789 4300 513
Size: 511 4296
Fragments arranged by size:
4296 939 681 552 511 444 219 183
135 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3905 487
Size: 683 56 2679 4665
Fragments arranged by size:
4665 2679 683 56
BtsI GCAGTG_nn'
Cuts at: 3560 3693 4159 4630 5599 6393 6413 3560
Size: 133 466 471 969 794 20 5230
Fragments arranged by size:
5230 969 794 471 466 133 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8083
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3471 3506 529
Size: 35 2907 35 5106
Fragments arranged by size:
5106 2907 35 35
DraI TTT'AAA
Cuts at: 5387 5387
Size: 8083
DraIII CAC_nnn'GTG
Cuts at: 2257 2257
Size: 8083
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3432 7090 809
Size: 507 2116 3658 1802
Fragments arranged by size:
3658 2116 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3439 4675 5530
Size: 54 1425 50 275 18 1609 1236 855
Cuts at: 5530 6419 8048 8
Size: 889 1629 43
Fragments arranged by size:
1629 1609 1425 1236 889 855 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5530 1537
Size: 275 3718 4090
Fragments arranged by size:
4090 3718 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4260 4428 4923 6013 1353
Size: 2907 168 495 1090 3423
Fragments arranged by size:
3423 2907 1090 495 168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6670 7044 7190 387
Size: 1064 138 5081 374 146 1280
Fragments arranged by size:
5081 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2637 2734 5213 2637
Size: 97 2479 5507
Fragments arranged by size:
5507 2479 97
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2204 2810 2840 2864 3080 3558 3785
Size: 393 572 606 30 24 216 478 227
Cuts at: 3785 3873 4071 4233 4313 4604 4736 4784 4980
Size: 88 198 162 80 291 132 48 196
Cuts at: 4980 5273 6146 6743 7530 7798 1239
Size: 293 873 597 787 268 1524
Fragments arranged by size:
1524 873 787 606 597 572 478 393
293 291 268 227 216 198 196 162
132 88 80 48 30 24
EcoO109I rG'GnC_Cy
Cuts at: 2106 2360 2367 2470 2567 2568 3425 3542 3543
Size: 254 7 103 97 1 857 117 1
Cuts at: 3543 3660 3755 3833 4224 4225 4242 4572 4781
Size: 117 95 78 391 1 17 330 209
Cuts at: 4781 5041 5158 5159 5248 2106
Size: 260 117 1 89 4941
Fragments arranged by size:
4941 857 391 330 260 254 209 117
117 117 103 97 95 89 78 17
7 1 1 1 1
EcoRI G'AATT_C
Cuts at: 4680 5392 5770 4680
Size: 712 378 6993
Fragments arranged by size:
6993 712 378
FspI TGC'GCA
Cuts at: 4539 6589 4539
Size: 2050 6033
HaeII r_GCGC'y
Cuts at: 1678 2875 3573 5347 7230 1678
Size: 1197 698 1774 1883 2531
Fragments arranged by size:
2531 1883 1774 1197 698
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2542 2574 3940 3972 4058
Size: 32 135 32 970 32 1366 32 86
Cuts at: 4058 4090 4922 4954 6724 6756 6798 6830 1373
Size: 32 832 32 1770 32 42 32 2626
Fragments arranged by size:
2626 1770 1366 970 832 135 86 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5427 5673 669
Size: 988 281 3489 246 3079
Fragments arranged by size:
3489 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2216 748
Size: 1468 6615
HpaI GTT'AAC
Cuts at: 5673 5673
Size: 8083
KasI G'GCGC_C
Cuts at: 3569 3569
Size: 8083
KpnI G_GTAC'C
Cuts at: 3585 5408 3585
Size: 1823 6260
MfeI C'AATT_G
Cuts at: 5682 5682
Size: 8083
MluI A'CGCG_T
Cuts at: 7959 7959
Size: 8083
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2389 3506 7197 7381 1305
Size: 1084 1117 3691 184 2007
Fragments arranged by size:
3691 2007 1117 1084 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3441 10
Size: 54 1425 343 1609 4652
Fragments arranged by size:
4652 1609 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2459 3051 3086 3333 3884 4400 4736
Size: 648 1293 592 35 247 551 516 336
Cuts at: 4736 6041 6400 6559 518
Size: 1305 359 159 2042
Fragments arranged by size:
2042 1305 1293 648 592 551 516 359
336 247 159 35
MspA1I CmG'CkG
Cuts at: 1606 2033 2756 2839 2947 3257 3737 4339 4891
Size: 427 723 83 108 310 480 602 552
Cuts at: 4891 5000 5021 5074 5167 5362 5499 6176 7324
Size: 109 21 53 93 195 137 677 1148
Cuts at: 7324 7569 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 723 677 602 552 480 427
310 245 195 137 109 108 93 83
53 21
NarI GG'CG_CC
Cuts at: 3570 3570
Size: 8083
NcoI C'CATG_G
Cuts at: 513 1065 2004 2685 3087 3789 4300 513
Size: 552 939 681 402 702 511 4296
Fragments arranged by size:
4296 939 702 681 552 511 402
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8083
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8083
NotI GC'GGCC_GC
Cuts at: 5530 5530
Size: 8083
NspI r_CATG'y
Cuts at: 1132 1229 3056 4544 4774 5441 6986 1132
Size: 97 1827 1488 230 667 1545 2229
Fragments arranged by size:
2229 1827 1545 1488 667 230 97
PasI CC'CwG_GG
Cuts at: 1348 3421 3448 4132 1348
Size: 2073 27 684 5299
Fragments arranged by size:
5299 2073 684 27
PciI A'CATG_T
Cuts at: 6982 6982
Size: 8083
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2257 3337 3448 3554 3851 4913 1108
Size: 1149 1080 111 106 297 1062 4278
Fragments arranged by size:
4278 1149 1080 1062 297 111 106
PfoI T'CCnGG_A
Cuts at: 2546 2826 3535 4048 2546
Size: 280 709 513 6581
Fragments arranged by size:
6581 709 513 280
PmeI GTTT'AAAC
Cuts at: 5387 5387
Size: 8083
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6152 6184 7665 7697 6152
Size: 32 1481 32 6538
Fragments arranged by size:
6538 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3425 3660 5041 5248 3425
Size: 235 1381 207 6260
Fragments arranged by size:
6260 1381 235 207
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8083
PsiI TTA'TAA
Cuts at: 5653 5653
Size: 8083
PspOMI G'GGCC_C
Cuts at: 2106 2567 2758 3542 4224 4572 5158 2106
Size: 461 191 784 682 348 586 5031
Fragments arranged by size:
5031 784 682 586 461 348 191
PstI C_TGCA'G
Cuts at: 830 3567 3841 5026 5319 5435 830
Size: 2737 274 1185 293 116 3478
Fragments arranged by size:
3478 2737 1185 293 274 116
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8083
PvuII CAG'CTG
Cuts at: 3257 3737 4339 5000 5021 5167 3257
Size: 480 602 661 21 146 6173
Fragments arranged by size:
6173 661 602 480 146 21
SacI G_AGCT'C
Cuts at: 2639 2736 5215 2639
Size: 97 2479 5507
Fragments arranged by size:
5507 2479 97
SalI G'TCGA_C
Cuts at: 1936 5425 1936
Size: 3489 4594
SanDI GG'GwC_CC
Cuts at: 3425 3660 5041 3425
Size: 235 1381 6467
Fragments arranged by size:
6467 1381 235
SbfI CC_TGCA'GG
Cuts at: 3841 5435 3841
Size: 1594 6489
ScaI AGT'ACT
Cuts at: 6331 8043 6331
Size: 1712 6371
SexAI A'CCwGG_T
Cuts at: 2253 2253
Size: 8083
SfcI C'TryA_G
Cuts at: 826 1045 2834 3563 3837 4776 5022 5278 5315
Size: 219 1789 729 274 939 246 256 37
Cuts at: 5315 5332 5431 6566 7247 7438 826
Size: 17 99 1135 681 191 1471
Fragments arranged by size:
1789 1471 1135 939 729 681 274 256
246 219 191 99 37 17
SfiI GGCCn_nnn'nGGCC
Cuts at: 3841 3841
Size: 8083
SfoI GGC'GCC
Cuts at: 3571 3571
Size: 8083
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8083
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8083
SmaI CCC'GGG
Cuts at: 1872 2492 2678 3488 4976 5410 1872
Size: 620 186 810 1488 434 4545
Fragments arranged by size:
4545 1488 810 620 434 186
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2632 4064 6187 7088 7350 820
Size: 197 926 689 1432 2123 901 262 1553
Fragments arranged by size:
2123 1553 1432 926 901 689 262 197
SnaBI TAC'GTA
Cuts at: 493 5874 493
Size: 5381 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8083
SphI G_CATG'C
Cuts at: 1132 5441 1132
Size: 4309 3774
SspI AAT'ATT
Cuts at: 5 52 6007 5
Size: 47 5955 2081
Fragments arranged by size:
5955 2081 47
StuI AGG'CCT
Cuts at: 1517 3215 4056 4602 1517
Size: 1698 841 546 4998
Fragments arranged by size:
4998 1698 841 546
StyI C'CwwG_G
Cuts at: 513 1065 2004 2073 2685 3087 3789 3916 4228
Size: 552 939 69 612 402 702 127 312
Cuts at: 4228 4300 4458 4597 4713 5012 5522 513
Size: 72 158 139 116 299 510 3074
Fragments arranged by size:
3074 939 702 612 552 510 402 312
299 158 139 127 116 72 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2803 2879 3787 4358 4402 6110 6127
Size: 1255 1223 76 908 571 44 1708 17
Cuts at: 6127 6280 6465 6910 325
Size: 153 185 445 1498
Fragments arranged by size:
1708 1498 1255 1223 908 571 445 185
153 76 44 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6329 8041 96
Size: 274 80 33 51 1443 4352 1712 138
Fragments arranged by size:
4352 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3123 3155 3444 3476 8043 8075 3123
Size: 32 289 32 4567 32 3131
Fragments arranged by size:
4567 3131 289 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2311 1316
Size: 995 7088
XbaI T'CTAG_A
Cuts at: 5419 5419
Size: 8083
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2032 3418 3592 3883 2032
Size: 1386 174 291 6232
Fragments arranged by size:
6232 1386 291 174
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8083
XmaI C'CCGG_G
Cuts at: 1870 2490 2676 3486 4974 5408 1870
Size: 620 186 810 1488 434 4545
Fragments arranged by size:
4545 1488 810 620 434 186
XmnI GAAnn'nnTTC
Cuts at: 6212 6212
Size: 8083
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5891 276
Size: 53 83 186 936 4357 2468
Fragments arranged by size:
4357 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoNI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SapI SrfI
SwaI