Restriction map for
pFN21AB4619
(Circular) (Six-base) MAPSORT of: pFN21AB4619.seq Check: 5757 from: 1 to: 5857
pFN21AB4619 5857 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3667 278
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2864 3200 1937
Size: 927 336 4594
Fragments arranged by size:
4594 927 336
Acc65I G'GTAC_C
Cuts at: 3178 3178
Size: 5857
AclI AA'CG_TT
Cuts at: 1563 3984 4357 1563
Size: 2421 373 3063
Fragments arranged by size:
3063 2421 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3920 5304 5572 1632
Size: 2288 1384 268 1917
Fragments arranged by size:
2288 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5660
AflIII A'CryG_T
Cuts at: 1203 2297 4756 5733 1203
Size: 1094 2459 977 1327
Fragments arranged by size:
2459 1327 1094 977
AgeI A'CCGG_T
Cuts at: 4688 4688
Size: 5857
AhdI GACnn_n'nnGTC
Cuts at: 4586 4586
Size: 5857
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5857
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5857
AlwNI CAG_nnn'CTG
Cuts at: 2339 3600 5172 2339
Size: 1261 1572 3024
Fragments arranged by size:
3024 1572 1261
ApaLI G'TGCA_C
Cuts at: 3914 5070 3914
Size: 1156 4701
ApoI r'AATT_y
Cuts at: 1493 2415 2738 3166 3392 3544 5653 1493
Size: 922 323 428 226 152 2109 1697
Fragments arranged by size:
2109 1697 922 428 323 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4411 160
Size: 872 3379 1606
Fragments arranged by size:
3379 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
AvaI C'yCGr_G
Cuts at: 1870 1943 3182 1870
Size: 73 1239 4545
Fragments arranged by size:
4545 1239 73
AvrII C'CTAG_G
Cuts at: 2909 2909
Size: 5857
BamHI G'GATC_C
Cuts at: 2314 2833 3187 3554 2314
Size: 519 354 367 4617
Fragments arranged by size:
4617 519 367 354
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3178 4633 618
Size: 325 217 627 1391 1455 1842
Fragments arranged by size:
1842 1455 1391 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2142 3176 1391
Size: 751 1034 4072
Fragments arranged by size:
4072 1034 751
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4886
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4048 4082 1903
Size: 34 2111 34 3678
Fragments arranged by size:
3678 2111 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2198 3749 4959 2198
Size: 1551 1210 3096
Fragments arranged by size:
3096 1551 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5857
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4468 136
Size: 107 122 71 631 605 2796 1525
Fragments arranged by size:
2796 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5852 5852
Size: 5857
BlpI GC'TnA_GC
Cuts at: 3274 3274
Size: 5857
Bme1580I G_kGCm'C
Cuts at: 1790 3918 5074 1790
Size: 2128 1156 2573
Fragments arranged by size:
2573 2128 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5857
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2834 3588 4546
Size: 416 53 83 399 24 1417 754 958
Cuts at: 4546 442
Size: 1753
Fragments arranged by size:
1753 1417 958 754 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5857
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2649 2871 2913 3123 4517 1239
Size: 1410 222 42 210 1394 2579
Fragments arranged by size:
2579 1410 1394 222 210 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3982 4847 5145 3982
Size: 865 298 4694
Fragments arranged by size:
4694 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4520 882
Size: 617 3021 2219
Fragments arranged by size:
3021 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3648 493
Size: 711 2444 2702
Fragments arranged by size:
2702 2444 711
BsaBI GATnn'nnATC
Cuts at: 2250 5851 2250
Size: 3601 2256
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2280 3664 4046 4681
Size: 53 83 186 936 747 1384 382 635
Cuts at: 4681 275
Size: 1451
Fragments arranged by size:
1451 1384 936 747 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4290 4688 4962 5109 5520 4290
Size: 398 274 147 411 4627
Fragments arranged by size:
4627 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2081 2111 2482 2512 2869
Size: 30 165 30 678 30 371 30 357
Cuts at: 2869 2899 3021 3051 1178
Size: 30 122 30 3984
Fragments arranged by size:
3984 678 371 357 165 122 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2126 2211 1186
Size: 940 85 4832
Fragments arranged by size:
4832 940 85
BseYI C'CCAG_C
Cuts at: 5060 5556 5060
Size: 496 5361
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2568 1163
Size: 573 832 4452
Fragments arranged by size:
4452 832 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2020 3307 4068 5096 1540
Size: 275 186 19 1287 761 1028 2301
Fragments arranged by size:
2301 1287 1028 761 275 186 19
BsiHKAI G_wGCw'C
Cuts at: 2142 2624 3918 4003 5074 2142
Size: 482 1294 85 1071 2925
Fragments arranged by size:
2925 1294 1071 482 85
BsmI GAATG_Cn'
Cuts at: 2774 3368 3461 2774
Size: 594 93 5170
Fragments arranged by size:
5170 594 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2624 3176 3918 4003 5074 1391
Size: 399 352 482 552 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 552 482 399 352 85
BspEI T'CCGG_A
Cuts at: 5520 5520
Size: 5857
BspHI T'CATG_A
Cuts at: 3746 5476 3746
Size: 1730 4127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3212 844
Size: 2368 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2457 3744 1394
Size: 429 634 1287 3507
Fragments arranged by size:
3507 1287 634 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2997 4352 4526 65
Size: 1183 1749 1355 174 1396
Fragments arranged by size:
1749 1396 1355 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4501 4688 1700
Size: 110 2691 187 2869
Fragments arranged by size:
2869 2691 187 110
BsrGI T'GTAC_A
Cuts at: 96 2489 96
Size: 2393 3464
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5857
BssSI C'ACGA_G
Cuts at: 3917 4929 3917
Size: 1012 4845
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2339 794
Size: 1545 4312
BstBI TT'CG_AA
Cuts at: 4661 4661
Size: 5857
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5857
BstYI r'GATC_y
Cuts at: 1972 2314 2596 2833 3187 3554 3939 3956 5408
Size: 342 282 237 354 367 385 17 1452
Cuts at: 5408 5494 5506 5852 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 354 346 342 282
237 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2902 2961 2902
Size: 59 5798
BtgI C'CryG_G
Cuts at: 513 1065 2004 2073 513
Size: 552 939 69 4297
Fragments arranged by size:
4297 939 552 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5118
Fragments arranged by size:
5118 683 56
BtsI GCAGTG_nn'
Cuts at: 3373 4167 4187 3373
Size: 794 20 5043
Fragments arranged by size:
5043 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5857
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5822
DraI TTT'AAA
Cuts at: 2322 3161 2322
Size: 839 5018
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2807 4864 809
Size: 507 1491 2057 1802
Fragments arranged by size:
2057 1802 1491 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2017 2070 2326
Size: 54 1425 50 275 18 187 53 256
Cuts at: 2326 3304 4193 5822 8
Size: 978 889 1629 43
Fragments arranged by size:
1629 1425 978 889 275 256 187 54
53 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2017 3304 1537
Size: 275 205 1287 4090
Fragments arranged by size:
4090 1287 275 205
EarI CTCTTCn'nnn_
Cuts at: 1353 3787 1353
Size: 2434 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4444 4818 4964 387
Size: 1064 138 2855 374 146 1280
Fragments arranged by size:
2855 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2140 2140
Size: 5857
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2649 2871 2913 3123 3920 4517 5304
Size: 393 1017 222 42 210 797 597 787
Cuts at: 5304 5572 1239
Size: 268 1524
Fragments arranged by size:
1524 1017 797 787 597 393 268 222
210 42
EcoNI CCTnn'n_nnAGG
Cuts at: 2964 2964
Size: 5857
EcoO109I rG'GnC_Cy
Cuts at: 2906 2906
Size: 5857
EcoRI G'AATT_C
Cuts at: 3166 3544 3166
Size: 378 5479
EcoRV GAT'ATC
Cuts at: 2721 2951 3062 2721
Size: 230 111 5516
Fragments arranged by size:
5516 230 111
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2990 2990
Size: 5857
FspI TGC'GCA
Cuts at: 4363 4363
Size: 5857
HaeII r_GCGC'y
Cuts at: 1678 2050 5004 1678
Size: 372 2954 2531
Fragments arranged by size:
2954 2531 372
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4498 4530 4572 4604 1373
Size: 32 135 32 2926 32 42 32 2626
Fragments arranged by size:
2926 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2865 3201 3447 669
Size: 988 281 927 336 246 3079
Fragments arranged by size:
3079 988 927 336 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5857
HpaI GTT'AAC
Cuts at: 3447 3447
Size: 5857
KpnI G_GTAC'C
Cuts at: 3182 3182
Size: 5857
MfeI C'AATT_G
Cuts at: 2805 3456 2805
Size: 651 5206
MluI A'CGCG_T
Cuts at: 5733 5733
Size: 5857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3007 4971 5155 1305
Size: 1702 1964 184 2007
Fragments arranged by size:
2007 1964 1702 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2328 10
Size: 54 1425 343 496 3539
Fragments arranged by size:
3539 1425 496 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3002 3815 4174 4333 518
Size: 648 1836 813 359 159 2042
Fragments arranged by size:
2042 1836 813 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3273 3950 5098 5343 1606
Size: 1667 677 1148 245 2120
Fragments arranged by size:
2120 1667 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2073 513
Size: 552 939 69 4297
Fragments arranged by size:
4297 939 552 69
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5857
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5857
NotI GC'GGCC_GC
Cuts at: 3304 3304
Size: 5857
NspI r_CATG'y
Cuts at: 1132 1229 2301 3215 4760 1132
Size: 97 1072 914 1545 2229
Fragments arranged by size:
2229 1545 1072 914 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5857
PciI A'CATG_T
Cuts at: 2297 4756 2297
Size: 2459 3398
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5857
PfoI T'CCnGG_A
Cuts at: 2944 2944
Size: 5857
PmeI GTTT'AAAC
Cuts at: 3161 3161
Size: 5857
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3926 3958 5439 5471 3926
Size: 32 1481 32 4312
Fragments arranged by size:
4312 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2906 2906
Size: 5857
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5857
PsiI TTA'TAA
Cuts at: 2190 3427 2190
Size: 1237 4620
PstI C_TGCA'G
Cuts at: 830 3209 830
Size: 2379 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5857
SacI G_AGCT'C
Cuts at: 2142 2142
Size: 5857
SalI G'TCGA_C
Cuts at: 1936 2863 3199 1936
Size: 927 336 4594
Fragments arranged by size:
4594 927 336
SbfI CC_TGCA'GG
Cuts at: 3209 3209
Size: 5857
ScaI AGT'ACT
Cuts at: 2827 4105 5817 2827
Size: 1278 1712 2867
Fragments arranged by size:
2867 1712 1278
SfcI C'TryA_G
Cuts at: 826 1045 3205 4340 5021 5212 826
Size: 219 2160 1135 681 191 1471
Fragments arranged by size:
2160 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5857
SmaI CCC'GGG
Cuts at: 1872 3184 1872
Size: 1312 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3961 4862 5124 820
Size: 197 926 2018 901 262 1553
Fragments arranged by size:
2018 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3648 493
Size: 3155 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5857
SphI G_CATG'C
Cuts at: 1132 3215 1132
Size: 2083 3774
SspI AAT'ATT
Cuts at: 5 52 3781 5
Size: 47 3729 2081
Fragments arranged by size:
3729 2081 47
StuI AGG'CCT
Cuts at: 1517 2914 1517
Size: 1397 4460
StyI C'CwwG_G
Cuts at: 513 1065 2004 2073 2909 3296 513
Size: 552 939 69 836 387 3074
Fragments arranged by size:
3074 939 836 552 387 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2246 3884 3901 4054 4239 4684 325
Size: 1255 666 1638 17 153 185 445 1498
Fragments arranged by size:
1638 1498 1255 666 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2088 2489 2825
Size: 274 80 33 51 1443 111 401 336
Cuts at: 2825 4103 5815 96
Size: 1278 1712 138
Fragments arranged by size:
1712 1443 1278 401 336 274 138 111
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5817 5849 5817
Size: 32 5825
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5857
XbaI T'CTAG_A
Cuts at: 3193 3193
Size: 5857
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5857
XmaI C'CCGG_G
Cuts at: 1870 3182 1870
Size: 1312 4545
XmnI GAAnn'nnTTC
Cuts at: 2415 3986 2415
Size: 1571 4286
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3665 276
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI