Restriction map for
pFN21AB4614
(Circular) (Six-base) MAPSORT of: pFN21AB4614.seq Check: 5196 from: 1 to: 6493
pFN21AB4614 6493 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4303 278
Size: 53 83 186 936 2767 2468
Fragments arranged by size:
2767 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3836 1937
Size: 1899 4594
Acc65I G'GTAC_C
Cuts at: 3814 3814
Size: 6493
AclI AA'CG_TT
Cuts at: 1563 4620 4993 1563
Size: 3057 373 3063
Fragments arranged by size:
3063 3057 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2829 2919 4556 5940 6208 1632
Size: 1197 90 1637 1384 268 1917
Fragments arranged by size:
1917 1637 1384 1197 268 90
AfeI AGC'GCT
Cuts at: 2590 2741 3142 2590
Size: 151 401 5941
Fragments arranged by size:
5941 401 151
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6296
AflIII A'CryG_T
Cuts at: 1203 3651 5392 6369 1203
Size: 2448 1741 977 1327
Fragments arranged by size:
2448 1741 1327 977
AgeI A'CCGG_T
Cuts at: 5324 5324
Size: 6493
AhdI GACnn_n'nnGTC
Cuts at: 5222 5222
Size: 6493
AleI CACnn'nnGTG
Cuts at: 1166 2636 3215 1166
Size: 1470 579 4444
Fragments arranged by size:
4444 1470 579
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2565 1184
Size: 1381 5112
AlwNI CAG_nnn'CTG
Cuts at: 4236 5808 4236
Size: 1572 4921
ApaLI G'TGCA_C
Cuts at: 4550 5706 4550
Size: 1156 5337
ApoI r'AATT_y
Cuts at: 1493 3332 3422 3629 3787 3802 4028 4180 6289
Size: 1839 90 207 158 15 226 152 2109
Cuts at: 6289 1493
Size: 1697
Fragments arranged by size:
2109 1839 1697 226 207 158 152 90
15
AseI AT'TA_AT
Cuts at: 160 1032 5047 160
Size: 872 4015 1606
Fragments arranged by size:
4015 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6493
AvaI C'yCGr_G
Cuts at: 1870 1943 2984 3818 1870
Size: 73 1041 834 4545
Fragments arranged by size:
4545 1041 834 73
BamHI G'GATC_C
Cuts at: 2684 3062 3823 4190 2684
Size: 378 761 367 4987
Fragments arranged by size:
4987 761 378 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2386 3163 3321 3814 5269
Size: 325 217 627 599 777 158 493 1455
Cuts at: 5269 618
Size: 1842
Fragments arranged by size:
1842 1455 777 627 599 493 325 217
158
BanII G_rGCy'C
Cuts at: 1391 3397 3812 1391
Size: 2006 415 4072
Fragments arranged by size:
4072 2006 415
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2403 2817 928
Size: 971 504 414 4604
Fragments arranged by size:
4604 971 504 414
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4684 4718 1903
Size: 34 2747 34 3678
Fragments arranged by size:
3678 2747 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2797 4385 5595 2797
Size: 1588 1210 3695
Fragments arranged by size:
3695 1588 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6493
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5104 136
Size: 107 122 71 631 605 3432 1525
Fragments arranged by size:
3432 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6488 6488
Size: 6493
BlpI GC'TnA_GC
Cuts at: 3228 3910 3228
Size: 682 5811
Bme1580I G_kGCm'C
Cuts at: 1790 2328 4554 5710 1790
Size: 538 2226 1156 2573
Fragments arranged by size:
2573 2226 1156 538
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6493
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2883 3317 4224
Size: 416 53 83 399 24 1466 434 907
Cuts at: 4224 5182 442
Size: 958 1753
Fragments arranged by size:
1753 1466 958 907 434 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2666 1056
Size: 1610 4883
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5153 1239
Size: 3914 2579
Bpu10I CC'TnA_GC
Cuts at: 2274 2274
Size: 6493
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4618 5483 5781 4618
Size: 865 298 5330
Fragments arranged by size:
5330 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5156 882
Size: 617 3657 2219
Fragments arranged by size:
3657 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4284 493
Size: 711 3080 2702
Fragments arranged by size:
3080 2702 711
BsaBI GATnn'nnATC
Cuts at: 6487 6487
Size: 6493
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2818 3072 4300 4682
Size: 53 83 186 936 1285 254 1228 382
Cuts at: 4682 5317 275
Size: 635 1451
Fragments arranged by size:
1451 1285 1228 936 635 382 254 186
83 53
BsaWI w'CCGG_w
Cuts at: 4926 5324 5598 5745 6156 4926
Size: 398 274 147 411 5263
Fragments arranged by size:
5263 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6268
Fragments arranged by size:
6268 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2505 3398 1186
Size: 1319 893 4281
Fragments arranged by size:
4281 1319 893
BseYI C'CCAG_C
Cuts at: 2035 2458 3192 5696 6192 2035
Size: 423 734 2504 496 2336
Fragments arranged by size:
2504 2336 734 496 423
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2247 2565 1163
Size: 573 511 318 5091
Fragments arranged by size:
5091 573 511 318
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3943 4704 5732 1540
Size: 275 186 1942 761 1028 2301
Fragments arranged by size:
2301 1942 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3234 4554 4639 5710 3234
Size: 1320 85 1071 4017
Fragments arranged by size:
4017 1320 1071 85
BsmI GAATG_Cn'
Cuts at: 4004 4097 4004
Size: 93 6400
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2328 3234 3397 3812 4554 4639 5710
Size: 399 538 906 163 415 742 85 1071
Cuts at: 5710 1391
Size: 2174
Fragments arranged by size:
2174 1071 906 742 538 415 399 163
85
BspEI T'CCGG_A
Cuts at: 6156 6156
Size: 6493
BspHI T'CATG_A
Cuts at: 4382 6112 4382
Size: 1730 4763
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3848 844
Size: 3004 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4380 1394
Size: 429 2557 3507
Fragments arranged by size:
3507 2557 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4988 5162 65
Size: 1183 3740 174 1396
Fragments arranged by size:
3740 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2278 5137 5324 1700
Size: 110 468 2859 187 2869
Fragments arranged by size:
2869 2859 468 187 110
BsrGI T'GTAC_A
Cuts at: 96 2222 96
Size: 2126 4367
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6493
BssSI C'ACGA_G
Cuts at: 4553 5565 4553
Size: 1012 5481
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2261 794
Size: 1467 5026
BstBI TT'CG_AA
Cuts at: 5297 5297
Size: 6493
BstEII G'GTnAC_C
Cuts at: 1184 3022 1184
Size: 1838 4655
BstXI CCAn_nnnn'nTGG
Cuts at: 2043 2873 2043
Size: 830 5663
BstYI r'GATC_y
Cuts at: 1972 2684 3062 3459 3823 4190 4575 4592 6044
Size: 712 378 397 364 367 385 17 1452
Cuts at: 6044 6130 6142 6488 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 712 397 385 378 367 364
346 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2390 2499 2615 3619 513
Size: 552 1325 109 116 1004 3387
Fragments arranged by size:
3387 1325 1004 552 116 109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2160 487
Size: 683 56 934 4820
Fragments arranged by size:
4820 934 683 56
BtsI GCAGTG_nn'
Cuts at: 3346 4009 4803 4823 3346
Size: 663 794 20 5016
Fragments arranged by size:
5016 794 663 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6493
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6458
DraI TTT'AAA
Cuts at: 3797 3797
Size: 6493
DraIII CAC_nnn'GTG
Cuts at: 2734 2734
Size: 6493
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5500 809
Size: 507 4184 1802
Fragments arranged by size:
4184 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3098 3940 4829
Size: 54 1425 50 275 18 1268 842 889
Cuts at: 4829 6458 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1268 889 842 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3940 1537
Size: 275 2128 4090
Fragments arranged by size:
4090 2128 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2674 2783 4423 1353
Size: 1321 109 1640 3423
Fragments arranged by size:
3423 1640 1321 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5080 5454 5600 387
Size: 1064 138 3491 374 146 1280
Fragments arranged by size:
3491 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2829 2919 4556 5153 5940 6208 1239
Size: 393 1197 90 1637 597 787 268 1524
Fragments arranged by size:
1637 1524 1197 787 597 393 268 90
EcoRI G'AATT_C
Cuts at: 3332 3802 4180 3332
Size: 470 378 5645
Fragments arranged by size:
5645 470 378
FspI TGC'GCA
Cuts at: 4999 4999
Size: 6493
HaeII r_GCGC'y
Cuts at: 1678 2592 2743 3144 5640 1678
Size: 914 151 401 2496 2531
Fragments arranged by size:
2531 2496 914 401 151
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3459 3491 5134 5166 5208
Size: 32 135 32 1887 32 1643 32 42
Cuts at: 5208 5240 1373
Size: 32 2626
Fragments arranged by size:
2626 1887 1643 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3837 4083 669
Size: 988 281 1899 246 3079
Fragments arranged by size:
3079 1899 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6493
HpaI GTT'AAC
Cuts at: 4083 4083
Size: 6493
KpnI G_GTAC'C
Cuts at: 3818 3818
Size: 6493
MfeI C'AATT_G
Cuts at: 4092 4092
Size: 6493
MluI A'CGCG_T
Cuts at: 6369 6369
Size: 6493
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5607 5791 1305
Size: 4302 184 2007
Fragments arranged by size:
4302 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4671
Fragments arranged by size:
4671 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2547 2636 2775 3215 3624 4451 4810
Size: 648 1381 89 139 440 409 827 359
Cuts at: 4810 4969 518
Size: 159 2042
Fragments arranged by size:
2042 1381 827 648 440 409 359 159
139 89
MspA1I CmG'CkG
Cuts at: 1606 3044 3909 4586 5734 5979 1606
Size: 1438 865 677 1148 245 2120
Fragments arranged by size:
2120 1438 1148 865 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3619 513
Size: 552 2554 3387
Fragments arranged by size:
3387 2554 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6493
NheI G'CTAG_C
Cuts at: 1052 2662 1052
Size: 1610 4883
NotI GC'GGCC_GC
Cuts at: 3940 3940
Size: 6493
NspI r_CATG'y
Cuts at: 1132 1229 2083 2486 2546 2983 3503 3655 3776
Size: 97 854 403 60 437 520 152 121
Cuts at: 3776 3851 5396 1132
Size: 75 1545 2229
Fragments arranged by size:
2229 1545 854 520 437 403 152 121
97 75 60
PasI CC'CwG_GG
Cuts at: 1348 2689 1348
Size: 1341 5152
PciI A'CATG_T
Cuts at: 3651 5392 3651
Size: 1741 4752
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2828 3008 1108
Size: 1720 180 4593
Fragments arranged by size:
4593 1720 180
PfoI T'CCnGG_A
Cuts at: 3657 3657
Size: 6493
PmeI GTTT'AAAC
Cuts at: 3797 3797
Size: 6493
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4562 4594 6075 6107 4562
Size: 32 1481 32 4948
Fragments arranged by size:
4948 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2817 1613
Size: 1204 5289
PsiI TTA'TAA
Cuts at: 4063 4063
Size: 6493
PstI C_TGCA'G
Cuts at: 830 2061 2605 3043 3845 830
Size: 1231 544 438 802 3478
Fragments arranged by size:
3478 1231 802 544 438
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6493
PvuII CAG'CTG
Cuts at: 3044 3044
Size: 6493
SalI G'TCGA_C
Cuts at: 1936 3835 1936
Size: 1899 4594
SbfI CC_TGCA'GG
Cuts at: 3845 3845
Size: 6493
ScaI AGT'ACT
Cuts at: 4741 6453 4741
Size: 1712 4781
SexAI A'CCwGG_T
Cuts at: 3276 3276
Size: 6493
SfcI C'TryA_G
Cuts at: 826 1045 2014 2057 2601 3039 3057 3841 4976
Size: 219 969 43 544 438 18 784 1135
Cuts at: 4976 5657 5848 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 969 784 681 544 438 219
191 43 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6493
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6493
SmaI CCC'GGG
Cuts at: 1872 3820 1872
Size: 1948 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4597 5498 5760 820
Size: 197 926 2654 901 262 1553
Fragments arranged by size:
2654 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4284 493
Size: 3791 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6493
SphI G_CATG'C
Cuts at: 1132 2546 2983 3851 1132
Size: 1414 437 868 3774
Fragments arranged by size:
3774 1414 868 437
SspI AAT'ATT
Cuts at: 5 52 4417 5
Size: 47 4365 2081
Fragments arranged by size:
4365 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6493
StyI C'CwwG_G
Cuts at: 513 1065 2042 2822 3106 3463 3619 3932 513
Size: 552 977 780 284 357 156 313 3074
Fragments arranged by size:
3074 977 780 552 357 313 284 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2281 3339 4520 4537 4690 4875 5320
Size: 1255 701 1058 1181 17 153 185 445
Cuts at: 5320 325
Size: 1498
Fragments arranged by size:
1498 1255 1181 1058 701 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2222 4739 6451
Size: 274 80 33 51 1443 245 2517 1712
Cuts at: 6451 96
Size: 138
Fragments arranged by size:
2517 1712 1443 274 245 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6453 6485 6453
Size: 32 6461
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6493
XbaI T'CTAG_A
Cuts at: 3829 3829
Size: 6493
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2368 3178 2368
Size: 810 5683
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6493
XmaI C'CCGG_G
Cuts at: 1870 3818 1870
Size: 1948 4545
XmnI GAAnn'nnTTC
Cuts at: 4622 4622
Size: 6493
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4301 276
Size: 53 83 186 936 2767 2468
Fragments arranged by size:
2767 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI