Restriction map for
pFN21AB4609
(Circular) (Six-base) MAPSORT of: pFN21AB4609.seq Check: 3258 from: 1 to: 5539
pFN21AB4609 5539 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3349 278
Size: 53 83 186 936 1813 2468
Fragments arranged by size:
2468 1813 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2882 1937
Size: 945 4594
Acc65I G'GTAC_C
Cuts at: 2860 2860
Size: 5539
AclI AA'CG_TT
Cuts at: 1563 3666 4039 1563
Size: 2103 373 3063
Fragments arranged by size:
3063 2103 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2309 2566 2791 3602 4986 5254 1632
Size: 677 257 225 811 1384 268 1917
Fragments arranged by size:
1917 1384 811 677 268 257 225
AfeI AGC'GCT
Cuts at: 2097 2429 2097
Size: 332 5207
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5342
AflIII A'CryG_T
Cuts at: 1203 4438 5415 1203
Size: 3235 977 1327
Fragments arranged by size:
3235 1327 977
AgeI A'CCGG_T
Cuts at: 4370 4370
Size: 5539
AhdI GACnn_n'nnGTC
Cuts at: 2054 4268 2054
Size: 2214 3325
AleI CACnn'nnGTG
Cuts at: 1166 2063 1166
Size: 897 4642
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2705 1184
Size: 1521 4018
AlwNI CAG_nnn'CTG
Cuts at: 2169 2491 3282 4854 2169
Size: 322 791 1572 2854
Fragments arranged by size:
2854 1572 791 322
ApaLI G'TGCA_C
Cuts at: 2594 2624 3596 4752 2594
Size: 30 972 1156 3381
Fragments arranged by size:
3381 1156 972 30
ApoI r'AATT_y
Cuts at: 1493 2848 3074 3226 5335 1493
Size: 1355 226 152 2109 1697
Fragments arranged by size:
2109 1697 1355 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2274 4093 160
Size: 872 1242 1819 1606
Fragments arranged by size:
1819 1606 1242 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5539
AvaI C'yCGr_G
Cuts at: 1870 1943 2864 1870
Size: 73 921 4545
Fragments arranged by size:
4545 921 73
BamHI G'GATC_C
Cuts at: 2203 2869 3236 2203
Size: 666 367 4506
Fragments arranged by size:
4506 666 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2411 2860 4315 618
Size: 325 217 627 624 449 1455 1842
Fragments arranged by size:
1842 1455 627 624 449 325 217
BanII G_rGCy'C
Cuts at: 1391 2142 2858 1391
Size: 751 716 4072
Fragments arranged by size:
4072 751 716
BbeI G_GCGC'C
Cuts at: 2415 2415
Size: 5539
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4568
BbvCI CC'TCA_GC
Cuts at: 2221 2221
Size: 5539
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2770 2804 3730 3764 1903
Size: 34 50 34 749 34 926 34 3678
Fragments arranged by size:
3678 926 749 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2227 3431 4641 2227
Size: 1204 1210 3125
Fragments arranged by size:
3125 1210 1204
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5539
BfrBI ATG'CAT
Cuts at: 2635 2635
Size: 5539
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2319 2373 4150
Size: 107 122 71 631 605 647 54 1777
Cuts at: 4150 136
Size: 1525
Fragments arranged by size:
1777 1525 647 631 605 122 107 71
54
BglII A'GATC_T
Cuts at: 5534 5534
Size: 5539
BlpI GC'TnA_GC
Cuts at: 2956 2956
Size: 5539
Bme1580I G_kGCm'C
Cuts at: 1790 2598 2628 3600 4756 1790
Size: 808 30 972 1156 2573
Fragments arranged by size:
2573 1156 972 808 30
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5539
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3270 4228 442
Size: 416 53 83 399 24 1853 958 1753
Fragments arranged by size:
1853 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5539
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4199 1239
Size: 2960 2579
Bpu10I CC'TnA_GC
Cuts at: 2015 2221 2015
Size: 206 5333
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3664 4529 4827 3664
Size: 865 298 4376
Fragments arranged by size:
4376 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4202 882
Size: 617 2703 2219
Fragments arranged by size:
2703 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2623 3330 493
Size: 711 1419 707 2702
Fragments arranged by size:
2702 1419 711 707
BsaBI GATnn'nnATC
Cuts at: 5533 5533
Size: 5539
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2412 3346 3728 4363
Size: 53 83 186 936 879 934 382 635
Cuts at: 4363 275
Size: 1451
Fragments arranged by size:
1451 936 934 879 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3972 4370 4644 4791 5202 3972
Size: 398 274 147 411 4309
Fragments arranged by size:
4309 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5314
Fragments arranged by size:
5314 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5539
BseYI C'CCAG_C
Cuts at: 2672 4742 5238 2672
Size: 2070 496 2973
Fragments arranged by size:
2973 2070 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2450 1163
Size: 573 714 4252
Fragments arranged by size:
4252 714 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2239 2544 2989 3750 4778 1540
Size: 275 186 238 305 445 761 1028 2301
Fragments arranged by size:
2301 1028 761 445 305 275 238 186
BsiHKAI G_wGCw'C
Cuts at: 2142 2598 2628 3600 3685 4756 2142
Size: 456 30 972 85 1071 2925
Fragments arranged by size:
2925 1071 972 456 85 30
BsiWI C'GTAC_G
Cuts at: 2619 2619
Size: 5539
BsmI GAATG_Cn'
Cuts at: 3050 3143 3050
Size: 93 5446
BsmBI CGTCTCn'nnnn_
Cuts at: 2609 2609
Size: 5539
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2598 2628 2858 3600 3685 4756
Size: 399 352 456 30 230 742 85 1071
Cuts at: 4756 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 456 399 352 230 85
30
BspEI T'CCGG_A
Cuts at: 5202 5202
Size: 5539
BspHI T'CATG_A
Cuts at: 3428 5158 3428
Size: 1730 3809
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2894 844
Size: 2050 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3426 1394
Size: 429 1603 3507
Fragments arranged by size:
3507 1603 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4034 4208 65
Size: 1183 2786 174 1396
Fragments arranged by size:
2786 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2543 4183 4370 1700
Size: 110 733 1640 187 2869
Fragments arranged by size:
2869 1640 733 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5539
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5539
BssSI C'ACGA_G
Cuts at: 3599 4611 3599
Size: 1012 4527
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2632 794
Size: 1838 3701
BstBI TT'CG_AA
Cuts at: 2499 4343 2499
Size: 1844 3695
BstEII G'GTnAC_C
Cuts at: 1184 2056 1184
Size: 872 4667
BstYI r'GATC_y
Cuts at: 1972 2124 2203 2869 3236 3621 3638 5090 5176
Size: 152 79 666 367 385 17 1452 86
Cuts at: 5176 5188 5534 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 666 385 367 346 152 86
79 17 12
Bsu36I CC'TnA_GG
Cuts at: 2370 2370
Size: 5539
BtgI C'CryG_G
Cuts at: 513 1065 2061 513
Size: 552 996 3991
Fragments arranged by size:
3991 996 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4800
Fragments arranged by size:
4800 683 56
BtsI GCAGTG_nn'
Cuts at: 2015 3055 3849 3869 2015
Size: 1040 794 20 3685
Fragments arranged by size:
3685 1040 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5539
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5504
DraI TTT'AAA
Cuts at: 2843 2843
Size: 5539
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4546 809
Size: 507 3230 1802
Fragments arranged by size:
3230 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2160 2236 2545
Size: 54 1425 50 275 18 330 76 309
Cuts at: 2545 2812 2986 3875 5504 8
Size: 267 174 889 1629 43
Fragments arranged by size:
1629 1425 889 330 309 275 267 174
76 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2236 2986 1537
Size: 275 424 750 4090
Fragments arranged by size:
4090 750 424 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2233 2501 2753 3469 1353
Size: 880 268 252 716 3423
Fragments arranged by size:
3423 880 716 268 252
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4126 4500 4646 387
Size: 1064 138 2537 374 146 1280
Fragments arranged by size:
2537 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2140 2140
Size: 5539
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2309 2566 2791 3602 4199 4986 5254
Size: 393 677 257 225 811 597 787 268
Cuts at: 5254 1239
Size: 1524
Fragments arranged by size:
1524 811 787 677 597 393 268 257
225
EcoNI CCTnn'n_nnAGG
Cuts at: 2822 2822
Size: 5539
EcoO109I rG'GnC_Cy
Cuts at: 2081 2194 2305 2348 2081
Size: 113 111 43 5272
Fragments arranged by size:
5272 113 111 43
EcoRI G'AATT_C
Cuts at: 2848 3226 2848
Size: 378 5161
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2449 2449
Size: 5539
FspI TGC'GCA
Cuts at: 4045 4045
Size: 5539
HaeII r_GCGC'y
Cuts at: 1678 2099 2415 2431 2758 4686 1678
Size: 421 316 16 327 1928 2531
Fragments arranged by size:
2531 1928 421 327 316 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2040 2072 4180 4212 4254
Size: 32 135 32 468 32 2108 32 42
Cuts at: 4254 4286 1373
Size: 32 2626
Fragments arranged by size:
2626 2108 468 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2883 3129 669
Size: 988 281 945 246 3079
Fragments arranged by size:
3079 988 945 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5539
HpaI GTT'AAC
Cuts at: 3129 3129
Size: 5539
KasI G'GCGC_C
Cuts at: 2411 2411
Size: 5539
KpnI G_GTAC'C
Cuts at: 2864 2864
Size: 5539
MfeI C'AATT_G
Cuts at: 3138 3138
Size: 5539
MluI A'CGCG_T
Cuts at: 5415 5415
Size: 5539
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4653 4837 1305
Size: 3348 184 2007
Fragments arranged by size:
3348 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2162 10
Size: 54 1425 343 330 3387
Fragments arranged by size:
3387 1425 343 330 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2063 3497 3856 4015 518
Size: 648 897 1434 359 159 2042
Fragments arranged by size:
2042 1434 897 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2166 2472 2601 2811 2955 3632 4780 5025
Size: 560 306 129 210 144 677 1148 245
Cuts at: 5025 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 560 306 245 210 144
129
NarI GG'CG_CC
Cuts at: 2412 2412
Size: 5539
NcoI C'CATG_G
Cuts at: 513 1065 2061 513
Size: 552 996 3991
Fragments arranged by size:
3991 996 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5539
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5539
NotI GC'GGCC_GC
Cuts at: 2986 2986
Size: 5539
NsiI A_TGCA'T
Cuts at: 2637 2637
Size: 5539
NspI r_CATG'y
Cuts at: 1132 1229 2897 4442 1132
Size: 97 1668 1545 2229
Fragments arranged by size:
2229 1668 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2077 1348
Size: 729 4810
PciI A'CATG_T
Cuts at: 4438 4438
Size: 5539
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4637
PmeI GTTT'AAAC
Cuts at: 2843 2843
Size: 5539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3608 3640 5121 5153 3608
Size: 32 1481 32 3994
Fragments arranged by size:
3994 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2081 2194 2305 2348 2081
Size: 113 111 43 5272
Fragments arranged by size:
5272 113 111 43
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5539
PsiI TTA'TAA
Cuts at: 3109 3109
Size: 5539
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2759 2791 2759
Size: 32 5507
PstI C_TGCA'G
Cuts at: 830 2891 830
Size: 2061 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5539
PvuII CAG'CTG
Cuts at: 2601 2601
Size: 5539
SacI G_AGCT'C
Cuts at: 2142 2142
Size: 5539
SalI G'TCGA_C
Cuts at: 1936 2881 1936
Size: 945 4594
SanDI GG'GwC_CC
Cuts at: 2081 2305 2348 2081
Size: 224 43 5272
Fragments arranged by size:
5272 224 43
SapI GCTCTTCn'nnn_
Cuts at: 2501 2753 2501
Size: 252 5287
SbfI CC_TGCA'GG
Cuts at: 2891 2891
Size: 5539
ScaI AGT'ACT
Cuts at: 3787 5499 3787
Size: 1712 3827
SfcI C'TryA_G
Cuts at: 826 1045 2776 2887 4022 4703 4894 826
Size: 219 1731 111 1135 681 191 1471
Fragments arranged by size:
1731 1471 1135 681 219 191 111
SfiI GGCCn_nnn'nGGCC
Cuts at: 2319 2319
Size: 5539
SfoI GGC'GCC
Cuts at: 2413 2413
Size: 5539
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5539
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5539
SmaI CCC'GGG
Cuts at: 1872 2866 1872
Size: 994 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3643 4544 4806 820
Size: 197 926 1700 901 262 1553
Fragments arranged by size:
1700 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3330 493
Size: 2837 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5539
SphI G_CATG'C
Cuts at: 1132 2897 1132
Size: 1765 3774
SspI AAT'ATT
Cuts at: 5 52 3463 5
Size: 47 3411 2081
Fragments arranged by size:
3411 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5539
StyI C'CwwG_G
Cuts at: 513 1065 2061 2353 2978 513
Size: 552 996 292 625 3074
Fragments arranged by size:
3074 996 625 552 292
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2514 2728 3566 3583 3736 3921 4366
Size: 1255 934 214 838 17 153 185 445
Cuts at: 4366 325
Size: 1498
Fragments arranged by size:
1498 1255 934 838 445 214 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3785 5497 96
Size: 274 80 33 51 1443 1808 1712 138
Fragments arranged by size:
1808 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5499 5531 5499
Size: 32 5507
Tth111I GACn'n_nGTC
Cuts at: 1316 2732 1316
Size: 1416 4123
XbaI T'CTAG_A
Cuts at: 2875 2875
Size: 5539
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2360 2360
Size: 5539
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5539
XmaI C'CCGG_G
Cuts at: 1870 2864 1870
Size: 994 4545
XmnI GAAnn'nnTTC
Cuts at: 2429 3668 2429
Size: 1239 4300
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3347 276
Size: 53 83 186 936 1813 2468
Fragments arranged by size:
2468 1813 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BplI BstXI
BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI
PacI PfoI PmlI PspOMI PspXI RsrII SacII SexAI
SrfI SwaI