Restriction map for
pFN21AB4592
(Circular) (Six-base) MAPSORT of: pFN21AB4592.seq Check: 98 from: 1 to: 6508
pFN21AB4592 6508 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4318 278
Size: 53 83 186 936 2782 2468
Fragments arranged by size:
2782 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3098 3405 3851 1937
Size: 1161 307 446 4594
Fragments arranged by size:
4594 1161 446 307
Acc65I G'GTAC_C
Cuts at: 3829 3829
Size: 6508
AclI AA'CG_TT
Cuts at: 1563 4635 5008 1563
Size: 3072 373 3063
Fragments arranged by size:
3072 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2664 2949 4571 5955 6223 1632
Size: 1032 285 1622 1384 268 1917
Fragments arranged by size:
1917 1622 1384 1032 285 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6311
AflIII A'CryG_T
Cuts at: 1203 3058 5407 6384 1203
Size: 1855 2349 977 1327
Fragments arranged by size:
2349 1855 1327 977
AgeI A'CCGG_T
Cuts at: 5339 5339
Size: 6508
AhdI GACnn_n'nnGTC
Cuts at: 5237 5237
Size: 6508
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6508
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6508
AlwNI CAG_nnn'CTG
Cuts at: 2945 4251 5823 2945
Size: 1306 1572 3630
Fragments arranged by size:
3630 1572 1306
ApaI G_GGCC'C
Cuts at: 3475 3475
Size: 6508
ApaLI G'TGCA_C
Cuts at: 4565 5721 4565
Size: 1156 5352
ApoI r'AATT_y
Cuts at: 1493 2263 2612 3015 3350 3370 3817 4043 4195
Size: 770 349 403 335 20 447 226 152
Cuts at: 4195 6304 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 770 447 403 349 335 226
152 20
AseI AT'TA_AT
Cuts at: 160 1032 3555 5062 160
Size: 872 2523 1507 1606
Fragments arranged by size:
2523 1606 1507 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6508
AvaI C'yCGr_G
Cuts at: 1870 1943 2024 3833 1870
Size: 73 81 1809 4545
Fragments arranged by size:
4545 1809 81 73
BamHI G'GATC_C
Cuts at: 3838 4205 3838
Size: 367 6141
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3829 5284 618
Size: 325 217 627 2042 1455 1842
Fragments arranged by size:
2042 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2025 2100 2850 3475 3827 1391
Size: 634 75 750 625 352 4072
Fragments arranged by size:
4072 750 634 625 352 75
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2115 3759 928
Size: 971 216 1644 3677
Fragments arranged by size:
3677 1644 971 216
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4699 4733 1903
Size: 34 2762 34 3678
Fragments arranged by size:
3678 2762 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3052 4400 5610 3052
Size: 1348 1210 3950
Fragments arranged by size:
3950 1348 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6508
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5119 136
Size: 107 122 71 631 605 3447 1525
Fragments arranged by size:
3447 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2382 6503 2382
Size: 4121 2387
BlpI GC'TnA_GC
Cuts at: 2250 2443 3925 2250
Size: 193 1482 4833
Fragments arranged by size:
4833 1482 193
Bme1580I G_kGCm'C
Cuts at: 1790 3475 4569 5725 1790
Size: 1685 1094 1156 2573
Fragments arranged by size:
2573 1685 1156 1094
BmgBI CAC'GTC
Cuts at: 1318 2113 2588 1318
Size: 795 475 5238
Fragments arranged by size:
5238 795 475
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2731 4239 5197
Size: 416 53 83 399 24 1314 1508 958
Cuts at: 5197 442
Size: 1753
Fragments arranged by size:
1753 1508 1314 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6508
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2834 5168 1239
Size: 1595 2334 2579
Fragments arranged by size:
2579 2334 1595
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2780 4633 5498 5796 2780
Size: 1853 865 298 3492
Fragments arranged by size:
3492 1853 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2990 3624 5171 882
Size: 617 1491 634 1547 2219
Fragments arranged by size:
2219 1547 1491 634 617
BsaAI yAC'GTr
Cuts at: 493 1204 4299 493
Size: 711 3095 2702
Fragments arranged by size:
3095 2702 711
BsaBI GATnn'nnATC
Cuts at: 3051 3227 6502 3051
Size: 176 3275 3057
Fragments arranged by size:
3275 3057 176
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4315 4697 5332 275
Size: 53 83 186 936 2782 382 635 1451
Fragments arranged by size:
2782 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4941 5339 5613 5760 6171 4941
Size: 398 274 147 411 5278
Fragments arranged by size:
5278 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3621 3651 1178
Size: 30 165 30 2218 30 4035
Fragments arranged by size:
4035 2218 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6508
BseYI C'CCAG_C
Cuts at: 2257 3340 5711 6207 2257
Size: 1083 2371 496 2558
Fragments arranged by size:
2558 2371 1083 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2135 1163
Size: 573 399 5536
Fragments arranged by size:
5536 573 399
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2079 3958 4719 5747 1540
Size: 275 186 78 1879 761 1028 2301
Fragments arranged by size:
2301 1879 1028 761 275 186 78
BsiHKAI G_wGCw'C
Cuts at: 2025 2100 2564 2850 4569 4654 5725 2025
Size: 75 464 286 1719 85 1071 2808
Fragments arranged by size:
2808 1719 1071 464 286 85 75
BsmI GAATG_Cn'
Cuts at: 4019 4112 4019
Size: 93 6415
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2100 2564 2850 3475 3827 4569
Size: 399 235 75 464 286 625 352 742
Cuts at: 4569 4654 5725 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 625 464 399 352 286
235 85 75
BspEI T'CCGG_A
Cuts at: 6171 6171
Size: 6508
BspHI T'CATG_A
Cuts at: 3438 4397 6127 3438
Size: 959 1730 3819
Fragments arranged by size:
3819 1730 959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3863 844
Size: 3019 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4395 1394
Size: 429 2572 3507
Fragments arranged by size:
3507 2572 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2110 5003 5177 65
Size: 1183 862 2893 174 1396
Fragments arranged by size:
2893 1396 1183 862 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2030 5152 5339 1700
Size: 110 220 3122 187 2869
Fragments arranged by size:
3122 2869 220 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6508
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6508
BssSI C'ACGA_G
Cuts at: 3392 4568 5580 3392
Size: 1176 1012 4320
Fragments arranged by size:
4320 1176 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2519 2945 3281 3604 794
Size: 1725 426 336 323 3698
Fragments arranged by size:
3698 1725 426 336 323
BstBI TT'CG_AA
Cuts at: 5312 5312
Size: 6508
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6508
BstYI r'GATC_y
Cuts at: 1972 2382 3838 4205 4590 4607 6059 6145 6157
Size: 410 1456 367 385 17 1452 86 12
Cuts at: 6157 6503 1972
Size: 346 1977
Fragments arranged by size:
1977 1456 1452 410 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3406 3406
Size: 6508
BtgI C'CryG_G
Cuts at: 513 1065 2004 2859 513
Size: 552 939 855 4162
Fragments arranged by size:
4162 939 855 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5769
Fragments arranged by size:
5769 683 56
BtsI GCAGTG_nn'
Cuts at: 4024 4818 4838 4024
Size: 794 20 5694
Fragments arranged by size:
5694 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6508
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6473
DraI TTT'AAA
Cuts at: 3812 3812
Size: 6508
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3761 5515 809
Size: 507 2445 1754 1802
Fragments arranged by size:
2445 1802 1754 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3303 3660 3955
Size: 54 1425 50 275 18 1473 357 295
Cuts at: 3955 4844 6473 8
Size: 889 1629 43
Fragments arranged by size:
1629 1473 1425 889 357 295 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3955 1537
Size: 275 2143 4090
Fragments arranged by size:
4090 2143 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2334 4438 1353
Size: 981 2104 3423
Fragments arranged by size:
3423 2104 981
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3287 5095 5469 5615 387
Size: 1064 138 1698 1808 374 146 1280
Fragments arranged by size:
1808 1698 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2023 2098 2848 2023
Size: 75 750 5683
Fragments arranged by size:
5683 750 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2664 2834 2949 4571 5168 5955 6223
Size: 393 1032 170 115 1622 597 787 268
Cuts at: 6223 1239
Size: 1524
Fragments arranged by size:
1622 1524 1032 787 597 393 268 170
115
EcoO109I rG'GnC_Cy
Cuts at: 2178 2285 2864 2178
Size: 107 579 5822
Fragments arranged by size:
5822 579 107
EcoRI G'AATT_C
Cuts at: 3350 3370 3817 4195 3350
Size: 20 447 378 5663
Fragments arranged by size:
5663 447 378 20
FspI TGC'GCA
Cuts at: 5014 5014
Size: 6508
HaeII r_GCGC'y
Cuts at: 1678 5655 1678
Size: 3977 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5149 5181 5223 5255 1373
Size: 32 135 32 3577 32 42 32 2626
Fragments arranged by size:
3577 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3852 4098 669
Size: 988 281 1914 246 3079
Fragments arranged by size:
3079 1914 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2415 2718 748
Size: 1667 303 4538
Fragments arranged by size:
4538 1667 303
HpaI GTT'AAC
Cuts at: 4098 4098
Size: 6508
KpnI G_GTAC'C
Cuts at: 3833 3833
Size: 6508
MfeI C'AATT_G
Cuts at: 2151 4107 2151
Size: 1956 4552
MluI A'CGCG_T
Cuts at: 6384 6384
Size: 6508
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2146 5622 5806 1305
Size: 841 3476 184 2007
Fragments arranged by size:
3476 2007 841 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3305 3662 10
Size: 54 1425 343 1473 357 2856
Fragments arranged by size:
2856 1473 1425 357 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2479 2576 3005 4466 4825 4984 518
Size: 648 1313 97 429 1461 359 159 2042
Fragments arranged by size:
2042 1461 1313 648 429 359 159 97
MspA1I CmG'CkG
Cuts at: 1606 2045 2257 2942 3325 3340 3924 4601 5749
Size: 439 212 685 383 15 584 677 1148
Cuts at: 5749 5994 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 685 677 584 439 383 245
212 15
NcoI C'CATG_G
Cuts at: 513 1065 2004 2859 513
Size: 552 939 855 4162
Fragments arranged by size:
4162 939 855 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6508
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6508
NotI GC'GGCC_GC
Cuts at: 3955 3955
Size: 6508
NspI r_CATG'y
Cuts at: 1132 1229 2484 3062 3866 5411 1132
Size: 97 1255 578 804 1545 2229
Fragments arranged by size:
2229 1545 1255 804 578 97
PasI CC'CwG_GG
Cuts at: 1348 2508 1348
Size: 1160 5348
PciI A'CATG_T
Cuts at: 3058 5407 3058
Size: 2349 4159
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6508
PmeI GTTT'AAAC
Cuts at: 3812 3812
Size: 6508
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4577 4609 6090 6122 4577
Size: 32 1481 32 4963
Fragments arranged by size:
4963 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2178 2285 2864 2178
Size: 107 579 5822
Fragments arranged by size:
5822 579 107
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6508
PsiI TTA'TAA
Cuts at: 4078 4078
Size: 6508
PspOMI G'GGCC_C
Cuts at: 3471 3471
Size: 6508
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3120 3152 3630 3662 3120
Size: 32 478 32 5966
Fragments arranged by size:
5966 478 32 32
PstI C_TGCA'G
Cuts at: 830 3860 830
Size: 3030 3478
PvuI CG_AT'CG
Cuts at: 2001 2079 2001
Size: 78 6430
PvuII CAG'CTG
Cuts at: 2257 2942 3340 2257
Size: 685 398 5425
Fragments arranged by size:
5425 685 398
SacI G_AGCT'C
Cuts at: 2025 2100 2850 2025
Size: 75 750 5683
Fragments arranged by size:
5683 750 75
SalI G'TCGA_C
Cuts at: 1936 3850 1936
Size: 1914 4594
SbfI CC_TGCA'GG
Cuts at: 3860 3860
Size: 6508
ScaI AGT'ACT
Cuts at: 3729 4756 6468 3729
Size: 1027 1712 3769
Fragments arranged by size:
3769 1712 1027
SfcI C'TryA_G
Cuts at: 826 1045 3731 3856 4991 5672 5863 826
Size: 219 2686 125 1135 681 191 1471
Fragments arranged by size:
2686 1471 1135 681 219 191 125
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6508
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6508
SmaI CCC'GGG
Cuts at: 1872 3835 1872
Size: 1963 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2759 4612 5513 5775 820
Size: 197 926 816 1853 901 262 1553
Fragments arranged by size:
1853 1553 926 901 816 262 197
SnaBI TAC'GTA
Cuts at: 493 4299 493
Size: 3806 2702
SpeI A'CTAG_T
Cuts at: 152 2669 3445 152
Size: 2517 776 3215
Fragments arranged by size:
3215 2517 776
SphI G_CATG'C
Cuts at: 1132 3866 1132
Size: 2734 3774
SspI AAT'ATT
Cuts at: 5 52 4432 5
Size: 47 4380 2081
Fragments arranged by size:
4380 2081 47
StuI AGG'CCT
Cuts at: 1517 3572 1517
Size: 2055 4453
StyI C'CwwG_G
Cuts at: 513 1065 2004 2859 3947 513
Size: 552 939 855 1088 3074
Fragments arranged by size:
3074 1088 939 855 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2597 3332 4535 4552 4705 4890 5335
Size: 1255 1017 735 1203 17 153 185 445
Cuts at: 5335 325
Size: 1498
Fragments arranged by size:
1498 1255 1203 1017 735 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3727 4754 6466
Size: 274 80 33 51 1443 1750 1027 1712
Cuts at: 6466 96
Size: 138
Fragments arranged by size:
1750 1712 1443 1027 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2170 2202 6468 6500 2170
Size: 32 4266 32 2178
Fragments arranged by size:
4266 2178 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6508
XbaI T'CTAG_A
Cuts at: 3691 3844 3691
Size: 153 6355
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3268 3268
Size: 6508
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6508
XmaI C'CCGG_G
Cuts at: 1870 3833 1870
Size: 1963 4545
XmnI GAAnn'nnTTC
Cuts at: 2655 3132 4637 2655
Size: 477 1505 4526
Fragments arranged by size:
4526 1505 477
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4316 276
Size: 53 83 186 936 2782 2468
Fragments arranged by size:
2782 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspXI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI