Restriction map for
pFN21AB4573
(Circular) (Six-base) MAPSORT of: pFN21AB4573.seq Check: 235 from: 1 to: 6649
pFN21AB4573 6649 bp
With 182 enzymes: SgfI *
October 20, 2009 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3309 3309
Size: 6649
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4459 278
Size: 53 83 186 936 2923 2468
Fragments arranged by size:
2923 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2774 3144 3597 3992 1937
Size: 837 370 453 395 4594
Fragments arranged by size:
4594 837 453 395 370
Acc65I G'GTAC_C
Cuts at: 3970 3970
Size: 6649
AclI AA'CG_TT
Cuts at: 1563 4776 5149 1563
Size: 3213 373 3063
Fragments arranged by size:
3213 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2465 2766 2859 3157 4712 6096 6364 1632
Size: 833 301 93 298 1555 1384 268 1917
Fragments arranged by size:
1917 1555 1384 833 301 298 268 93
AfeI AGC'GCT
Cuts at: 3862 3862
Size: 6649
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6452
AflIII A'CryG_T
Cuts at: 1203 5548 6525 1203
Size: 4345 977 1327
Fragments arranged by size:
4345 1327 977
AgeI A'CCGG_T
Cuts at: 5480 5480
Size: 6649
AhdI GACnn_n'nnGTC
Cuts at: 2646 5378 2646
Size: 2732 3917
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6649
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2828 1184
Size: 1644 5005
AlwNI CAG_nnn'CTG
Cuts at: 2969 3811 4392 5964 2969
Size: 842 581 1572 3654
Fragments arranged by size:
3654 1572 842 581
ApaLI G'TGCA_C
Cuts at: 4706 5862 4706
Size: 1156 5493
ApoI r'AATT_y
Cuts at: 1493 2474 2516 2596 3958 4184 4336 6445 1493
Size: 981 42 80 1362 226 152 2109 1697
Fragments arranged by size:
2109 1697 1362 981 226 152 80 42
AseI AT'TA_AT
Cuts at: 160 1032 5203 160
Size: 872 4171 1606
Fragments arranged by size:
4171 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6649
AvaI C'yCGr_G
Cuts at: 1870 1943 3931 3974 1870
Size: 73 1988 43 4545
Fragments arranged by size:
4545 1988 73 43
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2526 2559 2526
Size: 33 6616
BamHI G'GATC_C
Cuts at: 3979 4346 3979
Size: 367 6282
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2959 3970 5425 618
Size: 325 217 627 1172 1011 1455 1842
Fragments arranged by size:
1842 1455 1172 1011 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2909 3968 1391
Size: 1518 1059 4072
Fragments arranged by size:
4072 1518 1059
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2036 2441 3807 3937 928
Size: 971 137 405 1366 130 3640
Fragments arranged by size:
3640 1366 971 405 137 130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4840 4874 1903
Size: 34 2903 34 3678
Fragments arranged by size:
3678 2903 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4541 5751 4541
Size: 1210 5439
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6649
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2913 5260 136
Size: 107 122 71 631 605 1241 2347 1525
Fragments arranged by size:
2347 1525 1241 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3709 6644 3709
Size: 2935 3714
BlpI GC'TnA_GC
Cuts at: 4066 4066
Size: 6649
Bme1580I G_kGCm'C
Cuts at: 1790 2962 4710 5866 1790
Size: 1172 1748 1156 2573
Fragments arranged by size:
2573 1748 1172 1156
BmgBI CAC'GTC
Cuts at: 1318 3927 1318
Size: 2609 4040
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3262 4380 5338
Size: 416 53 83 399 24 1845 1118 958
Cuts at: 5338 442
Size: 1753
Fragments arranged by size:
1845 1753 1118 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6649
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3516 3516
Size: 6649
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2062 2584 2716 3511 5309 1239
Size: 823 522 132 795 1798 2579
Fragments arranged by size:
2579 1798 823 795 522 132
Bpu10I CC'TnA_GC
Cuts at: 3363 3363
Size: 6649
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2246 4774 5639 5937 2246
Size: 2528 865 298 2958
Fragments arranged by size:
2958 2528 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3308 5312 882
Size: 617 1809 2004 2219
Fragments arranged by size:
2219 2004 1809 617
BsaAI yAC'GTr
Cuts at: 493 1204 4440 493
Size: 711 3236 2702
Fragments arranged by size:
3236 2702 711
BsaBI GATnn'nnATC
Cuts at: 6643 6643
Size: 6649
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4456 4838 5473 275
Size: 53 83 186 936 2923 382 635 1451
Fragments arranged by size:
2923 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3855 5082 5480 5754 5901 6312 3855
Size: 1227 398 274 147 411 4192
Fragments arranged by size:
4192 1227 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3305 3335 1178
Size: 30 165 30 1902 30 4492
Fragments arranged by size:
4492 1902 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6649
BseYI C'CCAG_C
Cuts at: 2623 5852 6348 2623
Size: 3229 496 2924
Fragments arranged by size:
3229 2924 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2313 1163
Size: 573 577 5499
Fragments arranged by size:
5499 577 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4099 4860 5888 1540
Size: 275 186 2098 761 1028 2301
Fragments arranged by size:
2301 2098 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2013 2529 4710 4795 5866 2013
Size: 516 2181 85 1071 2796
Fragments arranged by size:
2796 2181 1071 516 85
BsmI GAATG_Cn'
Cuts at: 3564 4160 4253 3564
Size: 596 93 5960
Fragments arranged by size:
5960 596 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2529 2909 2962 3968 4710 4795
Size: 399 223 516 380 53 1006 742 85
Cuts at: 4795 5866 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1006 742 516 399 380 223
85 53
BspEI T'CCGG_A
Cuts at: 6312 6312
Size: 6649
BspHI T'CATG_A
Cuts at: 2521 4538 6268 2521
Size: 2017 1730 2902
Fragments arranged by size:
2902 2017 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3309 3496 4004 844
Size: 2465 187 508 3489
Fragments arranged by size:
3489 2465 508 187
BsrBI CCG'CTC
Cuts at: 1394 1823 2956 4536 1394
Size: 429 1133 1580 3507
Fragments arranged by size:
3507 1580 1133 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2047 3345 3750 5144 5318 65
Size: 1183 799 1298 405 1394 174 1396
Fragments arranged by size:
1396 1394 1298 1183 799 405 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5293 5480 1700
Size: 110 3483 187 2869
Fragments arranged by size:
3483 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6649
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6649
BssSI C'ACGA_G
Cuts at: 3000 4709 5721 3000
Size: 1709 1012 3928
Fragments arranged by size:
3928 1709 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6649
BstBI TT'CG_AA
Cuts at: 2848 5453 2848
Size: 2605 4044
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6649
BstXI CCAn_nnnn'nTGG
Cuts at: 2916 2916
Size: 6649
BstYI r'GATC_y
Cuts at: 1972 2152 3129 3709 3807 3979 4346 4731 4748
Size: 180 977 580 98 172 367 385 17
Cuts at: 4748 6200 6286 6298 6644 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 977 580 385 367 346 180
172 98 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5158
Fragments arranged by size:
5158 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5910
Fragments arranged by size:
5910 683 56
BtsI GCAGTG_nn'
Cuts at: 2301 4165 4959 4979 2301
Size: 1864 794 20 3971
Fragments arranged by size:
3971 1864 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6649
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3049 3084 529
Size: 35 2485 35 4094
Fragments arranged by size:
4094 2485 35 35
DraI TTT'AAA
Cuts at: 3953 3953
Size: 6649
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5656 809
Size: 507 4340 1802
Fragments arranged by size:
4340 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4096 4985 6614
Size: 54 1425 50 275 18 2266 889 1629
Cuts at: 6614 8
Size: 43
Fragments arranged by size:
2266 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4096 1537
Size: 275 2284 4090
Fragments arranged by size:
4090 2284 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3743 3780 4579 1353
Size: 2390 37 799 3423
Fragments arranged by size:
3423 2390 799 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5236 5610 5756 387
Size: 1064 138 3647 374 146 1280
Fragments arranged by size:
3647 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2062 2465 2584 2716 2766 2859 3157
Size: 393 430 403 119 132 50 93 298
Cuts at: 3157 3511 4712 5309 6096 6364 1239
Size: 354 1201 597 787 268 1524
Fragments arranged by size:
1524 1201 787 597 430 403 393 354
298 268 132 119 93 50
EcoO109I rG'GnC_Cy
Cuts at: 2272 2973 2272
Size: 701 5948
EcoRI G'AATT_C
Cuts at: 3958 4336 3958
Size: 378 6271
EcoRV GAT'ATC
Cuts at: 3904 3904
Size: 6649
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2470 2470
Size: 6649
FspI TGC'GCA
Cuts at: 3197 5155 3197
Size: 1958 4691
HaeII r_GCGC'y
Cuts at: 1678 2125 3864 5796 1678
Size: 447 1739 1932 2531
Fragments arranged by size:
2531 1932 1739 447
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2345 2377 2632 2664 3398
Size: 32 135 32 773 32 255 32 734
Cuts at: 3398 3430 3584 3616 3657 3689 5290 5322 5364
Size: 32 154 32 41 32 1601 32 42
Cuts at: 5364 5396 1373
Size: 32 2626
Fragments arranged by size:
2626 1601 773 734 255 154 135 42
41 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2775 3145 3993 4239 669
Size: 988 281 837 370 848 246 3079
Fragments arranged by size:
3079 988 848 837 370 281 246
HindIII A'AGCT_T
Cuts at: 748 2447 748
Size: 1699 4950
HpaI GTT'AAC
Cuts at: 4239 4239
Size: 6649
KpnI G_GTAC'C
Cuts at: 3974 3974
Size: 6649
MfeI C'AATT_G
Cuts at: 3258 4248 3258
Size: 990 5659
MluI A'CGCG_T
Cuts at: 6525 6525
Size: 6649
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3441 3570 3856 5763 5947 1305
Size: 2136 129 286 1907 184 2007
Fragments arranged by size:
2136 2007 1907 286 184 129
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4827
Fragments arranged by size:
4827 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3114 3303 3680 4607 4966 5125 518
Size: 648 1948 189 377 927 359 159 2042
Fragments arranged by size:
2042 1948 927 648 377 359 189 159
MspA1I CmG'CkG
Cuts at: 1606 2082 4065 4742 5890 6135 1606
Size: 476 1983 677 1148 245 2120
Fragments arranged by size:
2120 1983 1148 677 476 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5158
Fragments arranged by size:
5158 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6649
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6649
NotI GC'GGCC_GC
Cuts at: 4096 4096
Size: 6649
NspI r_CATG'y
Cuts at: 1132 1229 3212 3308 3386 4007 5552 1132
Size: 97 1983 96 78 621 1545 2229
Fragments arranged by size:
2229 1983 1545 621 97 96 78
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6649
PciI A'CATG_T
Cuts at: 5548 5548
Size: 6649
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2969 1108
Size: 1861 4788
PfoI T'CCnGG_A
Cuts at: 2394 2669 3309 2394
Size: 275 640 5734
Fragments arranged by size:
5734 640 275
PmeI GTTT'AAAC
Cuts at: 3953 3953
Size: 6649
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4718 4750 6231 6263 4718
Size: 32 1481 32 5104
Fragments arranged by size:
5104 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2973 2973
Size: 6649
PshAI GACnn'nnGTC
Cuts at: 1613 2466 1613
Size: 853 5796
PsiI TTA'TAA
Cuts at: 3096 4219 3096
Size: 1123 5526
PstI C_TGCA'G
Cuts at: 830 4001 830
Size: 3171 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6649
PvuII CAG'CTG
Cuts at: 2082 2082
Size: 6649
SalI G'TCGA_C
Cuts at: 1936 2773 3143 3991 1936
Size: 837 370 848 4594
Fragments arranged by size:
4594 848 837 370
SanDI GG'GwC_CC
Cuts at: 2973 2973
Size: 6649
SbfI CC_TGCA'GG
Cuts at: 4001 4001
Size: 6649
ScaI AGT'ACT
Cuts at: 4897 6609 4897
Size: 1712 4937
SfcI C'TryA_G
Cuts at: 826 1045 2530 3997 5132 5813 6004 826
Size: 219 1485 1467 1135 681 191 1471
Fragments arranged by size:
1485 1471 1467 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6649
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6649
SmaI CCC'GGG
Cuts at: 1872 3976 1872
Size: 2104 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2261 4753 5654 5916 820
Size: 197 926 318 2492 901 262 1553
Fragments arranged by size:
2492 1553 926 901 318 262 197
SnaBI TAC'GTA
Cuts at: 493 4440 493
Size: 3947 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6649
SphI G_CATG'C
Cuts at: 1132 3386 4007 1132
Size: 2254 621 3774
Fragments arranged by size:
3774 2254 621
SspI AAT'ATT
Cuts at: 5 52 4573 5
Size: 47 4521 2081
Fragments arranged by size:
4521 2081 47
StuI AGG'CCT
Cuts at: 1517 2998 3170 3793 1517
Size: 1481 172 623 4373
Fragments arranged by size:
4373 1481 623 172
StyI C'CwwG_G
Cuts at: 513 1065 2004 4088 513
Size: 552 939 2084 3074
Fragments arranged by size:
3074 2084 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4676 4693 4846 5031 5476 325
Size: 1255 3096 17 153 185 445 1498
Fragments arranged by size:
3096 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4895 6607 96
Size: 274 80 33 51 1443 2918 1712 138
Fragments arranged by size:
2918 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2719 2751 2775 2807 6609 6641 2719
Size: 32 24 32 3802 32 2727
Fragments arranged by size:
3802 2727 32 32 32 24
Tth111I GACn'n_nGTC
Cuts at: 1316 3925 1316
Size: 2609 4040
XbaI T'CTAG_A
Cuts at: 3985 3985
Size: 6649
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2815 2815
Size: 6649
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6649
XmaI C'CCGG_G
Cuts at: 1870 3974 1870
Size: 2104 4545
XmnI GAAnn'nnTTC
Cuts at: 2379 2438 4778 2379
Size: 59 2340 4250
Fragments arranged by size:
4250 2340 59
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4457 276
Size: 53 83 186 936 2923 2468
Fragments arranged by size:
2923 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbeI BbvCI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI
SfiI SfoI SrfI SwaI