Restriction map for
pFN21AB4571
(Circular) (Six-base) MAPSORT of: pFN21AB4571.seq Check: 7109 from: 1 to: 6550
pFN21AB4571 6550 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4360 278
Size: 53 83 186 936 2824 2468
Fragments arranged by size:
2824 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2269 2787 3893 1937
Size: 332 518 1106 4594
Fragments arranged by size:
4594 1106 518 332
Acc65I G'GTAC_C
Cuts at: 3871 3871
Size: 6550
AclI AA'CG_TT
Cuts at: 1563 4677 5050 1563
Size: 3114 373 3063
Fragments arranged by size:
3114 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2390 2969 4613 5997 6265 1632
Size: 758 579 1644 1384 268 1917
Fragments arranged by size:
1917 1644 1384 758 579 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6353
AflIII A'CryG_T
Cuts at: 1203 2967 5449 6426 1203
Size: 1764 2482 977 1327
Fragments arranged by size:
2482 1764 1327 977
AgeI A'CCGG_T
Cuts at: 5381 5381
Size: 6550
AhdI GACnn_n'nnGTC
Cuts at: 5279 5279
Size: 6550
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6550
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6550
AlwNI CAG_nnn'CTG
Cuts at: 4293 5865 4293
Size: 1572 4978
ApaLI G'TGCA_C
Cuts at: 4607 5763 4607
Size: 1156 5394
ApoI r'AATT_y
Cuts at: 1493 2018 2082 2459 2728 3517 3859 4085 4237
Size: 525 64 377 269 789 342 226 152
Cuts at: 4237 6346 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 789 525 377 342 269 226
152 64
AseI AT'TA_AT
Cuts at: 160 1032 5104 160
Size: 872 4072 1606
Fragments arranged by size:
4072 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6550
AvaI C'yCGr_G
Cuts at: 1870 1943 3875 1870
Size: 73 1932 4545
Fragments arranged by size:
4545 1932 73
BamHI G'GATC_C
Cuts at: 3880 4247 3880
Size: 367 6183
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3871 5326 618
Size: 325 217 627 2084 1455 1842
Fragments arranged by size:
2084 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3869 1391
Size: 2478 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2714 2881 928
Size: 971 815 167 4597
Fragments arranged by size:
4597 971 815 167
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4741 4775 1903
Size: 34 50 34 2720 34 3678
Fragments arranged by size:
3678 2720 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4442 5652 4442
Size: 1210 5340
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6550
BfrBI ATG'CAT
Cuts at: 3533 3533
Size: 6550
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5161 136
Size: 107 122 71 631 605 3489 1525
Fragments arranged by size:
3489 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6545 6545
Size: 6550
BlpI GC'TnA_GC
Cuts at: 3967 3967
Size: 6550
Bme1580I G_kGCm'C
Cuts at: 1790 2287 3825 4611 5767 1790
Size: 497 1538 786 1156 2573
Fragments arranged by size:
2573 1538 1156 786 497
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6550
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2712 3543 4281
Size: 416 53 83 399 24 1295 831 738
Cuts at: 4281 5239 442
Size: 958 1753
Fragments arranged by size:
1753 1295 958 831 738 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6550
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5210 1239
Size: 3971 2579
Bpu10I CC'TnA_GC
Cuts at: 2412 2412
Size: 6550
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2864 3043 4675 5540 5838 2864
Size: 179 1632 865 298 3576
Fragments arranged by size:
3576 1632 865 298 179
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5213 882
Size: 617 3714 2219
Fragments arranged by size:
3714 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2968 4341 493
Size: 711 1764 1373 2702
Fragments arranged by size:
2702 1764 1373 711
BsaBI GATnn'nnATC
Cuts at: 6544 6544
Size: 6550
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2227 4357 4739 5374
Size: 53 83 186 936 694 2130 382 635
Cuts at: 5374 275
Size: 1451
Fragments arranged by size:
2130 1451 936 694 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3537 4983 5381 5655 5802 6213 3537
Size: 1446 398 274 147 411 3874
Fragments arranged by size:
3874 1446 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2087 2117 3372 3402 1178
Size: 30 165 30 684 30 1255 30 4326
Fragments arranged by size:
4326 1255 684 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2649 2727 1186
Size: 1463 78 5009
Fragments arranged by size:
5009 1463 78
BseYI C'CCAG_C
Cuts at: 2106 5753 6249 2106
Size: 3647 496 2407
Fragments arranged by size:
3647 2407 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5977
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2269 4000 4761 5789 1540
Size: 275 186 268 1731 761 1028 2301
Fragments arranged by size:
2301 1731 1028 761 275 268 186
BsiHKAI G_wGCw'C
Cuts at: 4611 4696 5767 4611
Size: 85 1071 5394
Fragments arranged by size:
5394 1071 85
BsmI GAATG_Cn'
Cuts at: 3533 4061 4154 3533
Size: 528 93 5929
Fragments arranged by size:
5929 528 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2287 3825 3869 4611 4696 5767 1391
Size: 399 497 1538 44 742 85 1071 2174
Fragments arranged by size:
2174 1538 1071 742 497 399 85 44
BspEI T'CCGG_A
Cuts at: 3537 6213 3537
Size: 2676 3874
BspHI T'CATG_A
Cuts at: 4439 6169 4439
Size: 1730 4820
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3905 844
Size: 3061 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4437 1394
Size: 429 2614 3507
Fragments arranged by size:
3507 2614 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5045 5219 65
Size: 1183 3797 174 1396
Fragments arranged by size:
3797 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5194 5381 1700
Size: 110 3384 187 2869
Fragments arranged by size:
3384 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2970 96
Size: 2874 3676
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6550
BssSI C'ACGA_G
Cuts at: 3759 4610 5622 3759
Size: 851 1012 4687
Fragments arranged by size:
4687 1012 851
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6550
BstBI TT'CG_AA
Cuts at: 5354 5354
Size: 6550
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6550
BstXI CCAn_nnnn'nTGG
Cuts at: 3649 3649
Size: 6550
BstYI r'GATC_y
Cuts at: 1972 2713 3880 4247 4632 4649 6101 6187 6199
Size: 741 1167 367 385 17 1452 86 12
Cuts at: 6199 6545 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1167 741 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3336 513
Size: 552 2271 3727
Fragments arranged by size:
3727 2271 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5811
Fragments arranged by size:
5811 683 56
BtsI GCAGTG_nn'
Cuts at: 2589 4066 4860 4880 2589
Size: 1477 794 20 4259
Fragments arranged by size:
4259 1477 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6550
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6515
DraI TTT'AAA
Cuts at: 3573 3854 3573
Size: 281 6269
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5557 809
Size: 507 4241 1802
Fragments arranged by size:
4241 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3000 3115 3258
Size: 54 1425 50 275 18 1170 115 143
Cuts at: 3258 3455 3997 4886 6515 8
Size: 197 542 889 1629 43
Fragments arranged by size:
1629 1425 1170 889 542 275 197 143
115 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3997 1537
Size: 275 2185 4090
Fragments arranged by size:
4090 2185 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2512 2687 2948 4480 1353
Size: 1159 175 261 1532 3423
Fragments arranged by size:
3423 1532 1159 261 175
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5137 5511 5657 387
Size: 1064 138 3548 374 146 1280
Fragments arranged by size:
3548 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2390 2969 4613 5210 5997 6265 1239
Size: 393 758 579 1644 597 787 268 1524
Fragments arranged by size:
1644 1524 787 758 597 579 393 268
EcoRI G'AATT_C
Cuts at: 3859 4237 3859
Size: 378 6172
FspI TGC'GCA
Cuts at: 5056 5056
Size: 6550
HaeII r_GCGC'y
Cuts at: 1678 3059 5697 1678
Size: 1381 2638 2531
Fragments arranged by size:
2638 2531 1381
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2087 2119 2511 2543 3016
Size: 32 135 32 515 32 392 32 473
Cuts at: 3016 3048 5191 5223 5265 5297 1373
Size: 32 2143 32 42 32 2626
Fragments arranged by size:
2626 2143 515 473 392 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2270 3330 3894 4140 669
Size: 988 281 332 1060 564 246 3079
Fragments arranged by size:
3079 1060 988 564 332 281 246
HindIII A'AGCT_T
Cuts at: 748 3560 748
Size: 2812 3738
HpaI GTT'AAC
Cuts at: 4140 4140
Size: 6550
KpnI G_GTAC'C
Cuts at: 3875 3875
Size: 6550
MfeI C'AATT_G
Cuts at: 2832 4149 2832
Size: 1317 5233
MluI A'CGCG_T
Cuts at: 6426 6426
Size: 6550
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5664 5848 1305
Size: 4359 184 2007
Fragments arranged by size:
4359 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3260 3457 10
Size: 54 1425 343 1428 197 3103
Fragments arranged by size:
3103 1428 1425 343 197 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3441 4508 4867 5026 518
Size: 648 2275 1067 359 159 2042
Fragments arranged by size:
2275 2042 1067 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2362 3966 4643 5791 6036 1606
Size: 756 1604 677 1148 245 2120
Fragments arranged by size:
2120 1604 1148 756 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3336 513
Size: 552 2271 3727
Fragments arranged by size:
3727 2271 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6550
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6550
NotI GC'GGCC_GC
Cuts at: 3997 3997
Size: 6550
NsiI A_TGCA'T
Cuts at: 3535 3535
Size: 6550
NspI r_CATG'y
Cuts at: 1132 1229 3043 3908 5453 1132
Size: 97 1814 865 1545 2229
Fragments arranged by size:
2229 1814 1545 865 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6550
PciI A'CATG_T
Cuts at: 5449 5449
Size: 6550
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6550
PmeI GTTT'AAAC
Cuts at: 3854 3854
Size: 6550
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4619 4651 6132 6164 4619
Size: 32 1481 32 5005
Fragments arranged by size:
5005 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6550
PsiI TTA'TAA
Cuts at: 4120 4120
Size: 6550
PstI C_TGCA'G
Cuts at: 830 2926 3902 830
Size: 2096 976 3478
Fragments arranged by size:
3478 2096 976
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6550
SalI G'TCGA_C
Cuts at: 1936 2268 3892 1936
Size: 332 1624 4594
Fragments arranged by size:
4594 1624 332
SbfI CC_TGCA'GG
Cuts at: 3902 3902
Size: 6550
ScaI AGT'ACT
Cuts at: 3809 4798 6510 3809
Size: 989 1712 3849
Fragments arranged by size:
3849 1712 989
SexAI A'CCwGG_T
Cuts at: 2641 2641
Size: 6550
SfcI C'TryA_G
Cuts at: 826 1045 2922 3898 5033 5714 5905 826
Size: 219 1877 976 1135 681 191 1471
Fragments arranged by size:
1877 1471 1135 976 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6550
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6550
SmaI CCC'GGG
Cuts at: 1872 3877 1872
Size: 2005 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2879 3022 4654 5555 5817 820
Size: 197 926 936 143 1632 901 262 1553
Fragments arranged by size:
1632 1553 936 926 901 262 197 143
SnaBI TAC'GTA
Cuts at: 493 4341 493
Size: 3848 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6550
SphI G_CATG'C
Cuts at: 1132 3908 1132
Size: 2776 3774
SspI AAT'ATT
Cuts at: 5 52 4474 5
Size: 47 4422 2081
Fragments arranged by size:
4422 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6550
StyI C'CwwG_G
Cuts at: 513 1065 3255 3336 3669 3989 513
Size: 552 2190 81 333 320 3074
Fragments arranged by size:
3074 2190 552 333 320 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3181 3641 4577 4594 4747 4932 5377
Size: 1255 1601 460 936 17 153 185 445
Cuts at: 5377 325
Size: 1498
Fragments arranged by size:
1601 1498 1255 936 460 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2970 3807 4796
Size: 274 80 33 51 1443 993 837 989
Cuts at: 4796 6508 96
Size: 1712 138
Fragments arranged by size:
1712 1443 993 989 837 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3372 3404 6510 6542 3372
Size: 32 3106 32 3380
Fragments arranged by size:
3380 3106 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6550
XbaI T'CTAG_A
Cuts at: 3886 3886
Size: 6550
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3356 3356
Size: 6550
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6550
XmaI C'CCGG_G
Cuts at: 1870 3875 1870
Size: 2005 4545
XmnI GAAnn'nnTTC
Cuts at: 4679 4679
Size: 6550
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4358 276
Size: 53 83 186 936 2824 2468
Fragments arranged by size:
2824 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI