Restriction map for
pFN21AB4567
(Circular) (Six-base) MAPSORT of: pFN21AB4567.seq Check: 2787 from: 1 to: 6985
pFN21AB4567 6985 bp
With 182 enzymes: SgfI *
February 27, 2009 14:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3169 3220 3169
Size: 51 6934
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4795 278
Size: 53 83 186 936 3259 2468
Fragments arranged by size:
3259 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2764 4140 4328 1937
Size: 827 1376 188 4594
Fragments arranged by size:
4594 1376 827 188
Acc65I G'GTAC_C
Cuts at: 4306 4306
Size: 6985
AclI AA'CG_TT
Cuts at: 1563 5112 5485 1563
Size: 3549 373 3063
Fragments arranged by size:
3549 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2160 2343 2427 3702 3752 3779 4176 5048
Size: 528 183 84 1275 50 27 397 872
Cuts at: 5048 6432 6700 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1275 872 528 397 268 183
84 50 27
AflII C'TTAA_G
Cuts at: 820 1017 2594 820
Size: 197 1577 5211
Fragments arranged by size:
5211 1577 197
AflIII A'CryG_T
Cuts at: 1203 5884 6861 1203
Size: 4681 977 1327
Fragments arranged by size:
4681 1327 977
AgeI A'CCGG_T
Cuts at: 5816 5816
Size: 6985
AhdI GACnn_n'nnGTC
Cuts at: 5714 5714
Size: 6985
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6985
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6985
AlwNI CAG_nnn'CTG
Cuts at: 2942 4728 6300 2942
Size: 1786 1572 3627
Fragments arranged by size:
3627 1786 1572
ApaI G_GGCC'C
Cuts at: 2647 2647
Size: 6985
ApaLI G'TGCA_C
Cuts at: 5042 6198 5042
Size: 1156 5829
ApoI r'AATT_y
Cuts at: 1493 2901 4160 4294 4520 4672 6781 1493
Size: 1408 1259 134 226 152 2109 1697
Fragments arranged by size:
2109 1697 1408 1259 226 152 134
AseI AT'TA_AT
Cuts at: 160 1032 5539 160
Size: 872 4507 1606
Fragments arranged by size:
4507 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6985
AvaI C'yCGr_G
Cuts at: 1870 1943 4310 1870
Size: 73 2367 4545
Fragments arranged by size:
4545 2367 73
BamHI G'GATC_C
Cuts at: 4315 4682 4315
Size: 367 6618
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3157 4306 5761 618
Size: 325 217 627 1370 1149 1455 1842
Fragments arranged by size:
1842 1455 1370 1149 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2647 3821 4227 4304 1391
Size: 1256 1174 406 77 4072
Fragments arranged by size:
4072 1256 1174 406 77
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3197 3570 3690 928
Size: 971 1298 373 120 4223
Fragments arranged by size:
4223 1298 971 373 120
BbvCI CC'TCA_GC
Cuts at: 3590 3590
Size: 6985
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5176 5210 1903
Size: 34 3239 34 3678
Fragments arranged by size:
3678 3239 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4877 6087 4877
Size: 1210 5775
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6985
BfrBI ATG'CAT
Cuts at: 3296 3296
Size: 6985
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3673 5596 136
Size: 107 122 71 631 605 2001 1923 1525
Fragments arranged by size:
2001 1923 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2384 2673 3727 6980 2384
Size: 289 1054 3253 2389
Fragments arranged by size:
3253 2389 1054 289
BlpI GC'TnA_GC
Cuts at: 4402 4402
Size: 6985
Bme1580I G_kGCm'C
Cuts at: 1790 2647 2934 3381 5046 6202 1790
Size: 857 287 447 1665 1156 2573
Fragments arranged by size:
2573 1665 1156 857 447 287
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6985
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4716 5674 442
Size: 416 53 83 399 24 3299 958 1753
Fragments arranged by size:
3299 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6985
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2367 3094 3874 5645 1239
Size: 1128 727 780 1771 2579
Fragments arranged by size:
2579 1771 1128 780 727
Bpu10I CC'TnA_GC
Cuts at: 3504 3590 3504
Size: 86 6899
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5110 5975 6273 5110
Size: 865 298 5822
Fragments arranged by size:
5822 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3164 3430 3458 5648 882
Size: 617 1665 266 28 2190 2219
Fragments arranged by size:
2219 2190 1665 617 266 28
BsaAI yAC'GTr
Cuts at: 493 1204 4776 493
Size: 711 3572 2702
Fragments arranged by size:
3572 2702 711
BsaBI GATnn'nnATC
Cuts at: 3720 3726 6979 3720
Size: 6 3253 3726
Fragments arranged by size:
3726 3253 6
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4792 5174 5809 275
Size: 53 83 186 936 3259 382 635 1451
Fragments arranged by size:
3259 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5418 5816 6090 6237 6648 5418
Size: 398 274 147 411 5755
Fragments arranged by size:
5755 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4200 4230 1178
Size: 30 165 30 2797 30 3933
Fragments arranged by size:
3933 2797 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3120 3535 1186
Size: 1934 415 4636
Fragments arranged by size:
4636 1934 415
BseYI C'CCAG_C
Cuts at: 2187 2245 3444 6188 6684 2187
Size: 58 1199 2744 496 2488
Fragments arranged by size:
2744 2488 1199 496 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4077 1163
Size: 573 2341 4071
Fragments arranged by size:
4071 2341 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4435 5196 6224 1540
Size: 275 186 2434 761 1028 2301
Fragments arranged by size:
2434 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4227 5046 5131 6202 4227
Size: 819 85 1071 5010
Fragments arranged by size:
5010 1071 819 85
BsmI GAATG_Cn'
Cuts at: 4496 4589 4496
Size: 93 6892
BsmBI CGTCTCn'nnnn_
Cuts at: 3218 3218
Size: 6985
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2647 2934 3381 3821 4227 4304 5046
Size: 399 857 287 447 440 406 77 742
Cuts at: 5046 5131 6202 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 857 742 447 440 406 399
287 85 77
BspEI T'CCGG_A
Cuts at: 6648 6648
Size: 6985
BspHI T'CATG_A
Cuts at: 4874 6604 4874
Size: 1730 5255
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3169 3182 3220 4340 844
Size: 2325 13 38 1120 3489
Fragments arranged by size:
3489 2325 1120 38 13
BsrBI CCG'CTC
Cuts at: 1394 1823 4872 1394
Size: 429 3049 3507
Fragments arranged by size:
3507 3049 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2039 3147 5480 5654 65
Size: 1183 791 1108 2333 174 1396
Fragments arranged by size:
2333 1396 1183 1108 791 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5629 5816 1700
Size: 110 3819 187 2869
Fragments arranged by size:
3819 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6985
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6985
BssSI C'ACGA_G
Cuts at: 5045 6057 5045
Size: 1012 5973
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6985
BstBI TT'CG_AA
Cuts at: 5789 5789
Size: 6985
BstEII G'GTnAC_C
Cuts at: 1184 2397 1184
Size: 1213 5772
BstYI r'GATC_y
Cuts at: 1972 2384 2452 2673 3394 3727 4315 4682 5067
Size: 412 68 221 721 333 588 367 385
Cuts at: 5067 5084 6536 6622 6634 6980 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 721 588 412 385 367 346
333 221 86 68 17 12
Bsu36I CC'TnA_GG
Cuts at: 2943 3711 2943
Size: 768 6217
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5494
Fragments arranged by size:
5494 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6246
Fragments arranged by size:
6246 683 56
BtsI GCAGTG_nn'
Cuts at: 4501 5295 5315 4501
Size: 794 20 6171
Fragments arranged by size:
6171 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6985
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3601 3636 529
Size: 35 3037 35 3878
Fragments arranged by size:
3878 3037 35 35
DraI TTT'AAA
Cuts at: 3346 4289 3346
Size: 943 6042
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5992 809
Size: 507 4676 1802
Fragments arranged by size:
4676 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2817 3219 3602
Size: 54 1425 50 275 18 987 402 383
Cuts at: 3602 4432 5321 6950 8
Size: 830 889 1629 43
Fragments arranged by size:
1629 1425 987 889 830 402 383 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4432 1537
Size: 275 2620 4090
Fragments arranged by size:
4090 2620 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2156 3417 3870 3886 3992 4275 4915 1353
Size: 803 1261 453 16 106 283 640 3423
Fragments arranged by size:
3423 1261 803 640 453 283 106 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5572 5946 6092 387
Size: 1064 138 3983 374 146 1280
Fragments arranged by size:
3983 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4225 4225
Size: 6985
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2160 2343 2367 2427 3094 3702 3752
Size: 393 528 183 24 60 667 608 50
Cuts at: 3752 3779 3874 4176 5048 5645 6432 6700 1239
Size: 27 95 302 872 597 787 268 1524
Fragments arranged by size:
1524 872 787 667 608 597 528 393
302 268 183 95 60 50 27 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2417 2417
Size: 6985
EcoO109I rG'GnC_Cy
Cuts at: 2205 2939 2205
Size: 734 6251
EcoRI G'AATT_C
Cuts at: 4160 4294 4672 4160
Size: 134 378 6473
Fragments arranged by size:
6473 378 134
EcoRV GAT'ATC
Cuts at: 2429 2429
Size: 6985
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3346 3765 3346
Size: 419 6566
FspI TGC'GCA
Cuts at: 5491 5491
Size: 6985
HaeII r_GCGC'y
Cuts at: 1678 6132 1678
Size: 4454 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2376 2408 3184 3216 4200
Size: 32 135 32 804 32 776 32 984
Cuts at: 4200 4232 5626 5658 5700 5732 1373
Size: 32 1394 32 42 32 2626
Fragments arranged by size:
2626 1394 984 804 776 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2083 4329 4575 669
Size: 988 281 145 2246 246 3079
Fragments arranged by size:
3079 2246 988 281 246 145
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6985
HpaI GTT'AAC
Cuts at: 4575 4575
Size: 6985
KpnI G_GTAC'C
Cuts at: 4310 4310
Size: 6985
MfeI C'AATT_G
Cuts at: 4584 4584
Size: 6985
MluI A'CGCG_T
Cuts at: 6861 6861
Size: 6985
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6099 6283 1305
Size: 4794 184 2007
Fragments arranged by size:
4794 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2819 3221 3604 10
Size: 54 1425 343 987 402 383 3391
Fragments arranged by size:
3391 1425 987 402 383 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4943 5302 5461 518
Size: 648 3777 359 159 2042
Fragments arranged by size:
3777 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2138 2584 2834 3246 3575 4401 5078 6226
Size: 532 446 250 412 329 826 677 1148
Cuts at: 6226 6471 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 826 677 532 446 412 329
250 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5494
Fragments arranged by size:
5494 939 552
NdeI CA'TA_TG
Cuts at: 387 3292 387
Size: 2905 4080
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6985
NotI GC'GGCC_GC
Cuts at: 4432 4432
Size: 6985
NsiI A_TGCA'T
Cuts at: 3298 3298
Size: 6985
NspI r_CATG'y
Cuts at: 1132 1229 3862 4343 5888 1132
Size: 97 2633 481 1545 2229
Fragments arranged by size:
2633 2229 1545 481 97
PasI CC'CwG_GG
Cuts at: 1348 2240 3479 1348
Size: 892 1239 4854
Fragments arranged by size:
4854 1239 892
PciI A'CATG_T
Cuts at: 5884 5884
Size: 6985
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4192 1108
Size: 3084 3901
PmeI GTTT'AAAC
Cuts at: 4289 4289
Size: 6985
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5054 5086 6567 6599 5054
Size: 32 1481 32 5440
Fragments arranged by size:
5440 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2205 2939 2205
Size: 734 6251
PshAI GACnn'nnGTC
Cuts at: 1613 2395 1613
Size: 782 6203
PsiI TTA'TAA
Cuts at: 4555 4555
Size: 6985
PspOMI G'GGCC_C
Cuts at: 2643 2643
Size: 6985
PstI C_TGCA'G
Cuts at: 830 4337 830
Size: 3507 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6985
PvuII CAG'CTG
Cuts at: 2138 2834 3246 3575 2138
Size: 696 412 329 5548
Fragments arranged by size:
5548 696 412 329
SacI G_AGCT'C
Cuts at: 4227 4227
Size: 6985
SalI G'TCGA_C
Cuts at: 1936 4327 1936
Size: 2391 4594
SanDI GG'GwC_CC
Cuts at: 2205 2205
Size: 6985
SbfI CC_TGCA'GG
Cuts at: 4337 4337
Size: 6985
ScaI AGT'ACT
Cuts at: 5233 6945 5233
Size: 1712 5273
SfcI C'TryA_G
Cuts at: 826 1045 3783 4333 5468 6149 6340 826
Size: 219 2738 550 1135 681 191 1471
Fragments arranged by size:
2738 1471 1135 681 550 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6985
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6985
SmaI CCC'GGG
Cuts at: 1872 4312 1872
Size: 2440 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2594 5089 5990 6252 820
Size: 197 926 651 2495 901 262 1553
Fragments arranged by size:
2495 1553 926 901 651 262 197
SnaBI TAC'GTA
Cuts at: 493 4776 493
Size: 4283 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6985
SphI G_CATG'C
Cuts at: 1132 4343 1132
Size: 3211 3774
SspI AAT'ATT
Cuts at: 5 52 2115 4909 5
Size: 47 2063 2794 2081
Fragments arranged by size:
2794 2081 2063 47
StuI AGG'CCT
Cuts at: 1517 3542 1517
Size: 2025 4960
StyI C'CwwG_G
Cuts at: 513 1065 2004 3537 3639 3924 4424 513
Size: 552 939 1533 102 285 500 3074
Fragments arranged by size:
3074 1533 939 552 500 285 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2972 3500 3875 5012 5029 5182 5367
Size: 1255 1392 528 375 1137 17 153 185
Cuts at: 5367 5812 325
Size: 445 1498
Fragments arranged by size:
1498 1392 1255 1137 528 445 375 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5231 6943 96
Size: 274 80 33 51 1443 3254 1712 138
Fragments arranged by size:
3254 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6945 6977 6945
Size: 32 6953
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6985
XbaI T'CTAG_A
Cuts at: 2387 2695 3724 4321 2387
Size: 308 1029 597 5051
Fragments arranged by size:
5051 1029 597 308
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2342 2342
Size: 6985
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6985
XmaI C'CCGG_G
Cuts at: 1870 4310 1870
Size: 2440 4545
XmnI GAAnn'nnTTC
Cuts at: 5114 5114
Size: 6985
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4793 276
Size: 53 83 186 936 3259 2468
Fragments arranged by size:
3259 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI
BstXI BstZ17I DraIII FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PspXI PsrI RsrII
SacII SapI SexAI SfiI SfoI SrfI SwaI