Restriction map for
pFN21AB4540
(Circular) (Six-base) MAPSORT of: pFN21AB4540.seq Check: 9080 from: 1 to: 5749
pFN21AB4540 5749 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3559 278
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2157 3092 1937
Size: 220 935 4594
Fragments arranged by size:
4594 935 220
Acc65I G'GTAC_C
Cuts at: 3070 3070
Size: 5749
AclI AA'CG_TT
Cuts at: 1563 3876 4249 1563
Size: 2313 373 3063
Fragments arranged by size:
3063 2313 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2387 3812 5196 5464 1632
Size: 755 1425 1384 268 1917
Fragments arranged by size:
1917 1425 1384 755 268
AflII C'TTAA_G
Cuts at: 820 1017 2015 820
Size: 197 998 4554
Fragments arranged by size:
4554 998 197
AflIII A'CryG_T
Cuts at: 1203 2888 4648 5625 1203
Size: 1685 1760 977 1327
Fragments arranged by size:
1760 1685 1327 977
AgeI A'CCGG_T
Cuts at: 4580 4580
Size: 5749
AhdI GACnn_n'nnGTC
Cuts at: 4478 4478
Size: 5749
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5749
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5749
AlwNI CAG_nnn'CTG
Cuts at: 2292 3492 5064 2292
Size: 1200 1572 2977
Fragments arranged by size:
2977 1572 1200
ApaLI G'TGCA_C
Cuts at: 3806 4962 3806
Size: 1156 4593
ApoI r'AATT_y
Cuts at: 1493 3058 3284 3436 5545 1493
Size: 1565 226 152 2109 1697
Fragments arranged by size:
2109 1697 1565 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2696 4303 160
Size: 872 1664 1607 1606
Fragments arranged by size:
1664 1607 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
AvaI C'yCGr_G
Cuts at: 1870 1943 3074 1870
Size: 73 1131 4545
Fragments arranged by size:
4545 1131 73
BamHI G'GATC_C
Cuts at: 3079 3446 3079
Size: 367 5382
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3070 4525 618
Size: 325 217 627 1283 1455 1842
Fragments arranged by size:
1842 1455 1283 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2064 3068 1391
Size: 673 1004 4072
Fragments arranged by size:
4072 1004 673
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3051 928
Size: 971 1152 3626
Fragments arranged by size:
3626 1152 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3940 3974 1903
Size: 34 50 34 1919 34 3678
Fragments arranged by size:
3678 1919 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3641 4851 3641
Size: 1210 4539
BclI T'GATC_A
Cuts at: 1548 2239 1548
Size: 691 5058
BfrBI ATG'CAT
Cuts at: 2322 2322
Size: 5749
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4360 136
Size: 107 122 71 631 605 2688 1525
Fragments arranged by size:
2688 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5744 5744
Size: 5749
BlpI GC'TnA_GC
Cuts at: 2247 3166 2247
Size: 919 4830
Bme1580I G_kGCm'C
Cuts at: 1790 2438 3810 4966 1790
Size: 648 1372 1156 2573
Fragments arranged by size:
2573 1372 1156 648
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5749
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3480 4438 442
Size: 416 53 83 399 24 2063 958 1753
Fragments arranged by size:
2063 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5749
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2842 2842
Size: 5749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2498 2599 4409 1239
Size: 1259 101 1810 2579
Fragments arranged by size:
2579 1810 1259 101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2048 2088 3874 4739 5037 2048
Size: 40 1786 865 298 2760
Fragments arranged by size:
2760 1786 865 298 40
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2018 2671 4412 882
Size: 617 519 653 1741 2219
Fragments arranged by size:
2219 1741 653 617 519
BsaAI yAC'GTr
Cuts at: 493 1204 3540 493
Size: 711 2336 2702
Fragments arranged by size:
2702 2336 711
BsaBI GATnn'nnATC
Cuts at: 5743 5743
Size: 5749
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3556 3938 4573 275
Size: 53 83 186 936 2023 382 635 1451
Fragments arranged by size:
2023 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4182 4580 4854 5001 5412 4182
Size: 398 274 147 411 4519
Fragments arranged by size:
4519 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5524
Fragments arranged by size:
5524 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2151 2501 1186
Size: 965 350 4434
Fragments arranged by size:
4434 965 350
BseYI C'CCAG_C
Cuts at: 2308 4952 5448 2308
Size: 2644 496 2609
Fragments arranged by size:
2644 2609 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2108 2210 2305 2359 2581 2665 1163
Size: 573 372 102 95 54 222 84 4247
Fragments arranged by size:
4247 573 372 222 102 95 84 54
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2561 3199 3960 4988 1540
Size: 275 186 560 638 761 1028 2301
Fragments arranged by size:
2301 1028 761 638 560 275 186
BsiHKAI G_wGCw'C
Cuts at: 2064 3810 3895 4966 2064
Size: 1746 85 1071 2847
Fragments arranged by size:
2847 1746 1071 85
BsmI GAATG_Cn'
Cuts at: 2324 3260 3353 2324
Size: 936 93 4720
Fragments arranged by size:
4720 936 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2438 3068 3810 3895 4966 1391
Size: 399 274 374 630 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 630 399 374 274 85
BspEI T'CCGG_A
Cuts at: 5412 5412
Size: 5749
BspHI T'CATG_A
Cuts at: 3638 5368 3638
Size: 1730 4019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2637 2727 3104 844
Size: 1793 90 377 3489
Fragments arranged by size:
3489 1793 377 90
BsrBI CCG'CTC
Cuts at: 1394 1823 3636 1394
Size: 429 1813 3507
Fragments arranged by size:
3507 1813 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4244 4418 65
Size: 1183 2996 174 1396
Fragments arranged by size:
2996 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2991 4393 4580 1700
Size: 110 1181 1402 187 2869
Fragments arranged by size:
2869 1402 1181 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5749
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5749
BssSI C'ACGA_G
Cuts at: 3809 4821 3809
Size: 1012 4737
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5749
BstBI TT'CG_AA
Cuts at: 4553 4553
Size: 5749
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5749
BstYI r'GATC_y
Cuts at: 1972 2147 3079 3446 3831 3848 5300 5386 5398
Size: 175 932 367 385 17 1452 86 12
Cuts at: 5398 5744 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 932 385 367 346 175 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2601 2659 513
Size: 552 1536 58 3603
Fragments arranged by size:
3603 1536 552 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5010
Fragments arranged by size:
5010 683 56
BtsI GCAGTG_nn'
Cuts at: 2193 3265 4059 4079 2193
Size: 1072 794 20 3863
Fragments arranged by size:
3863 1072 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5749
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5714
DraI TTT'AAA
Cuts at: 3053 3053
Size: 5749
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4756 809
Size: 507 3440 1802
Fragments arranged by size:
3440 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2398 2445 2558
Size: 54 1425 50 275 18 568 47 113
Cuts at: 2558 3196 4085 5714 8
Size: 638 889 1629 43
Fragments arranged by size:
1629 1425 889 638 568 275 113 54
50 47 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2558 3196 1537
Size: 275 746 638 4090
Fragments arranged by size:
4090 746 638 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2016 2787 3679 1353
Size: 663 771 892 3423
Fragments arranged by size:
3423 892 771 663
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4336 4710 4856 387
Size: 1064 138 2747 374 146 1280
Fragments arranged by size:
2747 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2062 2062
Size: 5749
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2387 2498 2599 3812 4409 5196 5464
Size: 393 755 111 101 1213 597 787 268
Cuts at: 5464 1239
Size: 1524
Fragments arranged by size:
1524 1213 787 755 597 393 268 111
101
EcoRI G'AATT_C
Cuts at: 3058 3436 3058
Size: 378 5371
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2392 2392
Size: 5749
FspI TGC'GCA
Cuts at: 4255 4255
Size: 5749
HaeII r_GCGC'y
Cuts at: 1678 4896 1678
Size: 3218 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2059 2091 2186 2218 4390
Size: 32 135 32 487 32 95 32 2172
Cuts at: 4390 4422 4464 4496 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2172 487 135 95 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2652 3016 3093 3339 669
Size: 988 281 714 364 77 246 3079
Fragments arranged by size:
3079 988 714 364 281 246 77
HindIII A'AGCT_T
Cuts at: 748 2106 748
Size: 1358 4391
HpaI GTT'AAC
Cuts at: 3339 3339
Size: 5749
KpnI G_GTAC'C
Cuts at: 3074 3074
Size: 5749
MfeI C'AATT_G
Cuts at: 3348 3348
Size: 5749
MluI A'CGCG_T
Cuts at: 5625 5625
Size: 5749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2897 4863 5047 1305
Size: 1592 1966 184 2007
Fragments arranged by size:
2007 1966 1592 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2400 2447 10
Size: 54 1425 343 568 47 3312
Fragments arranged by size:
3312 1425 568 343 54 47
MslI CAynn'nnrTG
Cuts at: 518 1166 2964 3707 4066 4225 518
Size: 648 1798 743 359 159 2042
Fragments arranged by size:
2042 1798 743 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2572 2619 3165 3842 4990 5235 1606
Size: 966 47 546 677 1148 245 2120
Fragments arranged by size:
2120 1148 966 677 546 245 47
NcoI C'CATG_G
Cuts at: 513 1065 2601 2659 513
Size: 552 1536 58 3603
Fragments arranged by size:
3603 1536 552 58
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5749
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5749
NotI GC'GGCC_GC
Cuts at: 3196 3196
Size: 5749
NsiI A_TGCA'T
Cuts at: 2324 2324
Size: 5749
NspI r_CATG'y
Cuts at: 1132 1229 2326 2348 2892 3107 4652 1132
Size: 97 1097 22 544 215 1545 2229
Fragments arranged by size:
2229 1545 1097 544 215 97 22
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5749
PciI A'CATG_T
Cuts at: 2888 4648 2888
Size: 1760 3989
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2846 1108
Size: 1738 4011
PfoI T'CCnGG_A
Cuts at: 2220 2220
Size: 5749
PmeI GTTT'AAAC
Cuts at: 3053 3053
Size: 5749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3818 3850 5331 5363 3818
Size: 32 1481 32 4204
Fragments arranged by size:
4204 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5749
PsiI TTA'TAA
Cuts at: 3319 3319
Size: 5749
PstI C_TGCA'G
Cuts at: 830 2689 3101 830
Size: 1859 412 3478
Fragments arranged by size:
3478 1859 412
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5749
PvuII CAG'CTG
Cuts at: 2572 2619 2572
Size: 47 5702
SacI G_AGCT'C
Cuts at: 2064 2064
Size: 5749
SalI G'TCGA_C
Cuts at: 1936 3091 1936
Size: 1155 4594
SbfI CC_TGCA'GG
Cuts at: 3101 3101
Size: 5749
ScaI AGT'ACT
Cuts at: 3997 5709 3997
Size: 1712 4037
SfcI C'TryA_G
Cuts at: 826 1045 2685 3097 4232 4913 5104 826
Size: 219 1640 412 1135 681 191 1471
Fragments arranged by size:
1640 1471 1135 681 412 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5749
SmaI CCC'GGG
Cuts at: 1872 3076 1872
Size: 1204 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2015 2063 2103 3853 4754 5016
Size: 197 926 72 48 40 1750 901 262
Cuts at: 5016 820
Size: 1553
Fragments arranged by size:
1750 1553 926 901 262 197 72 48
40
SnaBI TAC'GTA
Cuts at: 493 3540 493
Size: 3047 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5749
SphI G_CATG'C
Cuts at: 1132 3107 1132
Size: 1975 3774
SspI AAT'ATT
Cuts at: 5 52 2044 2909 3673 5
Size: 47 1992 865 764 2081
Fragments arranged by size:
2081 1992 865 764 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5749
StyI C'CwwG_G
Cuts at: 513 1065 2523 2601 2659 3188 513
Size: 552 1458 78 58 529 3074
Fragments arranged by size:
3074 1458 552 529 78 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3776 3793 3946 4131 4576 325
Size: 1255 2196 17 153 185 445 1498
Fragments arranged by size:
2196 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2325 2349 2547
Size: 274 80 33 51 1443 348 24 198
Cuts at: 2547 2636 2891 3995 5707 96
Size: 89 255 1104 1712 138
Fragments arranged by size:
1712 1443 1104 348 274 255 198 138
89 80 51 33 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5709 5741 5709
Size: 32 5717
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5749
XbaI T'CTAG_A
Cuts at: 2939 3085 2939
Size: 146 5603
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2393 2393
Size: 5749
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5749
XmaI C'CCGG_G
Cuts at: 1870 3074 1870
Size: 1204 4545
XmnI GAAnn'nnTTC
Cuts at: 3878 3878
Size: 5749
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3557 276
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI