Restriction map for
pFN21AB4538
(Circular) (Six-base) MAPSORT of: pFN21AB4538.seq Check: 4954 from: 1 to: 5896
pFN21AB4538 5896 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2020 3706 278
Size: 53 83 186 936 484 1686 2468
Fragments arranged by size:
2468 1686 936 484 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3239 1937
Size: 1302 4594
Acc65I G'GTAC_C
Cuts at: 3217 3217
Size: 5896
AclI AA'CG_TT
Cuts at: 1563 4023 4396 1563
Size: 2460 373 3063
Fragments arranged by size:
3063 2460 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2690 2885 3008 3046 3959 5343 5611 1632
Size: 1058 195 123 38 913 1384 268 1917
Fragments arranged by size:
1917 1384 1058 913 268 195 123 38
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5699
AflIII A'CryG_T
Cuts at: 1203 4795 5772 1203
Size: 3592 977 1327
Fragments arranged by size:
3592 1327 977
AgeI A'CCGG_T
Cuts at: 4727 4727
Size: 5896
AhdI GACnn_n'nnGTC
Cuts at: 4625 4625
Size: 5896
AleI CACnn'nnGTG
Cuts at: 1166 2315 1166
Size: 1149 4747
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5896
AlwNI CAG_nnn'CTG
Cuts at: 2717 3639 5211 2717
Size: 922 1572 3402
Fragments arranged by size:
3402 1572 922
ApaLI G'TGCA_C
Cuts at: 3953 5109 3953
Size: 1156 4740
ApoI r'AATT_y
Cuts at: 1493 2600 3205 3431 3583 5692 1493
Size: 1107 605 226 152 2109 1697
Fragments arranged by size:
2109 1697 1107 605 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4450 160
Size: 872 3418 1606
Fragments arranged by size:
3418 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5896
AvaI C'yCGr_G
Cuts at: 1870 1943 2168 3221 1870
Size: 73 225 1053 4545
Fragments arranged by size:
4545 1053 225 73
BamHI G'GATC_C
Cuts at: 3226 3593 3226
Size: 367 5529
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3217 4672 618
Size: 325 217 627 1430 1455 1842
Fragments arranged by size:
1842 1455 1430 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3215 1391
Size: 1824 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2053 2533 928
Size: 971 154 480 4291
Fragments arranged by size:
4291 971 480 154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4087 4121 1903
Size: 34 2150 34 3678
Fragments arranged by size:
3678 2150 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2051 3788 4998 2051
Size: 1737 1210 2949
Fragments arranged by size:
2949 1737 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5896
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4507 136
Size: 107 122 71 631 605 2835 1525
Fragments arranged by size:
2835 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2809 3115 5891 2809
Size: 306 2776 2814
Fragments arranged by size:
2814 2776 306
BlpI GC'TnA_GC
Cuts at: 3313 3313
Size: 5896
Bme1580I G_kGCm'C
Cuts at: 1790 3957 5113 1790
Size: 2167 1156 2573
Fragments arranged by size:
2573 2167 1156
BmgBI CAC'GTC
Cuts at: 1318 2313 1318
Size: 995 4901
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3627 4585 442
Size: 416 53 83 399 24 2210 958 1753
Fragments arranged by size:
2210 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5896
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4556 1239
Size: 3317 2579
Bpu10I CC'TnA_GC
Cuts at: 2225 2225
Size: 5896
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3129 4021 4886 5184 3129
Size: 892 865 298 3841
Fragments arranged by size:
3841 892 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3171 4559 882
Size: 617 1672 1388 2219
Fragments arranged by size:
2219 1672 1388 617
BsaAI yAC'GTr
Cuts at: 493 1204 3687 493
Size: 711 2483 2702
Fragments arranged by size:
2702 2483 711
BsaBI GATnn'nnATC
Cuts at: 5890 5890
Size: 5896
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2017 3703 4085 4720
Size: 53 83 186 936 484 1686 382 635
Cuts at: 4720 275
Size: 1451
Fragments arranged by size:
1686 1451 936 635 484 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4329 4727 5001 5148 5559 4329
Size: 398 274 147 411 4666
Fragments arranged by size:
4666 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3089 3119 1178
Size: 30 165 30 1686 30 3955
Fragments arranged by size:
3955 1686 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3095 1186
Size: 1909 3987
BseYI C'CCAG_C
Cuts at: 2548 5099 5595 2548
Size: 2551 496 2849
Fragments arranged by size:
2849 2551 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5323
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3346 4107 5135 1540
Size: 275 186 1345 761 1028 2301
Fragments arranged by size:
2301 1345 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3184 3957 4042 5113 3184
Size: 773 85 1071 3967
Fragments arranged by size:
3967 1071 773 85
BsmI GAATG_Cn'
Cuts at: 3407 3500 3407
Size: 93 5803
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3184 3215 3957 4042 5113 1391
Size: 399 1394 31 742 85 1071 2174
Fragments arranged by size:
2174 1394 1071 742 399 85 31
BspEI T'CCGG_A
Cuts at: 5559 5559
Size: 5896
BspHI T'CATG_A
Cuts at: 3785 5515 3785
Size: 1730 4166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3251 844
Size: 2407 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3783 1394
Size: 429 1960 3507
Fragments arranged by size:
3507 1960 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4391 4565 65
Size: 1183 3143 174 1396
Fragments arranged by size:
3143 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2205 4540 4727 1700
Size: 110 395 2335 187 2869
Fragments arranged by size:
2869 2335 395 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5896
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5896
BssSI C'ACGA_G
Cuts at: 3956 4968 3956
Size: 1012 4884
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5896
BstBI TT'CG_AA
Cuts at: 4700 4700
Size: 5896
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5896
BstXI CCAn_nnnn'nTGG
Cuts at: 2840 2840
Size: 5896
BstYI r'GATC_y
Cuts at: 1972 2809 3115 3226 3593 3978 3995 5447 5533
Size: 837 306 111 367 385 17 1452 86
Cuts at: 5533 5545 5891 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 837 385 367 346 306 111
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2303 2954 2303
Size: 651 5245
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5344
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2751 487
Size: 683 56 1525 3632
Fragments arranged by size:
3632 1525 683 56
BtsI GCAGTG_nn'
Cuts at: 2390 3412 4206 4226 2390
Size: 1022 794 20 4060
Fragments arranged by size:
4060 1022 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5896
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5861
DraI TTT'AAA
Cuts at: 2353 3189 3200 2353
Size: 836 11 5049
Fragments arranged by size:
5049 836 11
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2306 4903 809
Size: 507 990 2597 1802
Fragments arranged by size:
2597 1802 990 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2941 3343 4232
Size: 54 1425 50 275 18 1111 402 889
Cuts at: 4232 5861 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1111 889 402 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3343 1537
Size: 275 1531 4090
Fragments arranged by size:
4090 1531 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2819 3826 1353
Size: 1466 1007 3423
Fragments arranged by size:
3423 1466 1007
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4483 4857 5003 387
Size: 1064 138 2894 374 146 1280
Fragments arranged by size:
2894 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2690 2885 3008 3046 3959 4556 5343
Size: 393 1058 195 123 38 913 597 787
Cuts at: 5343 5611 1239
Size: 268 1524
Fragments arranged by size:
1524 1058 913 787 597 393 268 195
123 38
EcoRI G'AATT_C
Cuts at: 3205 3583 3205
Size: 378 5518
EcoRV GAT'ATC
Cuts at: 2157 3076 2157
Size: 919 4977
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2645 2645
Size: 5896
FspI TGC'GCA
Cuts at: 4402 4402
Size: 5896
HaeII r_GCGC'y
Cuts at: 1678 5043 1678
Size: 3365 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3149 3181 4537 4569 4611
Size: 32 135 32 1577 32 1356 32 42
Cuts at: 4611 4643 1373
Size: 32 2626
Fragments arranged by size:
2626 1577 1356 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3240 3486 669
Size: 988 281 1302 246 3079
Fragments arranged by size:
3079 1302 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5896
HpaI GTT'AAC
Cuts at: 3486 3486
Size: 5896
KpnI G_GTAC'C
Cuts at: 3221 3221
Size: 5896
MfeI C'AATT_G
Cuts at: 3495 3495
Size: 5896
MluI A'CGCG_T
Cuts at: 5772 5772
Size: 5896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1993 5010 5194 1305
Size: 688 3017 184 2007
Fragments arranged by size:
3017 2007 688 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4074
Fragments arranged by size:
4074 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2315 2630 3854 4213 4372 518
Size: 648 1149 315 1224 359 159 2042
Fragments arranged by size:
2042 1224 1149 648 359 315 159
MspA1I CmG'CkG
Cuts at: 1606 3312 3989 5137 5382 1606
Size: 1706 677 1148 245 2120
Fragments arranged by size:
2120 1706 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5344
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5896
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5896
NotI GC'GGCC_GC
Cuts at: 3343 3343
Size: 5896
NspI r_CATG'y
Cuts at: 1132 1229 2233 3254 4799 1132
Size: 97 1004 1021 1545 2229
Fragments arranged by size:
2229 1545 1021 1004 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5896
PciI A'CATG_T
Cuts at: 4795 4795
Size: 5896
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2530 1108
Size: 1422 4474
PfoI T'CCnGG_A
Cuts at: 2487 2487
Size: 5896
PmeI GTTT'AAAC
Cuts at: 3200 3200
Size: 5896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3965 3997 5478 5510 3965
Size: 32 1481 32 4351
Fragments arranged by size:
4351 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5896
PsiI TTA'TAA
Cuts at: 3466 3466
Size: 5896
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2195 2227 2195
Size: 32 5864
PstI C_TGCA'G
Cuts at: 830 3248 830
Size: 2418 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5896
SalI G'TCGA_C
Cuts at: 1936 3238 1936
Size: 1302 4594
SbfI CC_TGCA'GG
Cuts at: 3248 3248
Size: 5896
ScaI AGT'ACT
Cuts at: 2701 2870 4144 5856 2701
Size: 169 1274 1712 2741
Fragments arranged by size:
2741 1712 1274 169
SexAI A'CCwGG_T
Cuts at: 2976 2976
Size: 5896
SfcI C'TryA_G
Cuts at: 826 1045 2641 3244 4379 5060 5251 826
Size: 219 1596 603 1135 681 191 1471
Fragments arranged by size:
1596 1471 1135 681 603 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5896
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5896
SmaI CCC'GGG
Cuts at: 1872 3223 1872
Size: 1351 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3144 4000 4901 5163 820
Size: 197 926 1201 856 901 262 1553
Fragments arranged by size:
1553 1201 926 901 856 262 197
SnaBI TAC'GTA
Cuts at: 493 3687 493
Size: 3194 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5896
SphI G_CATG'C
Cuts at: 1132 2233 3254 1132
Size: 1101 1021 3774
Fragments arranged by size:
3774 1101 1021
SspI AAT'ATT
Cuts at: 5 52 3820 5
Size: 47 3768 2081
Fragments arranged by size:
3768 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5896
StyI C'CwwG_G
Cuts at: 513 1065 3335 513
Size: 552 2270 3074
Fragments arranged by size:
3074 2270 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2774 3923 3940 4093 4278 4723 325
Size: 1255 1194 1149 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1194 1149 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2211 2699 2868
Size: 274 80 33 51 1443 234 488 169
Cuts at: 2868 3127 4142 5854 96
Size: 259 1015 1712 138
Fragments arranged by size:
1712 1443 1015 488 274 259 234 169
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5856 5888 5856
Size: 32 5864
Tth111I GACn'n_nGTC
Cuts at: 1316 2978 1316
Size: 1662 4234
XbaI T'CTAG_A
Cuts at: 3232 3232
Size: 5896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2108 2108
Size: 5896
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5896
XmaI C'CCGG_G
Cuts at: 1870 3221 1870
Size: 1351 4545
XmnI GAAnn'nnTTC
Cuts at: 2429 4025 2429
Size: 1596 4300
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2018 3704 276
Size: 53 83 186 936 484 1686 2468
Fragments arranged by size:
2468 1686 936 484 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI
PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PvuII
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI