Restriction map for
pFN21AB4518
(Circular) (Six-base) MAPSORT of: pFN21AB4518.seq Check: 4794 from: 1 to: 5719
pFN21AB4518 5719 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2333 2333
Size: 5719
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3529 278
Size: 53 83 186 936 1993 2468
Fragments arranged by size:
2468 1993 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3062 1937
Size: 1125 4594
Acc65I G'GTAC_C
Cuts at: 3040 3040
Size: 5719
AclI AA'CG_TT
Cuts at: 1563 3846 4219 1563
Size: 2283 373 3063
Fragments arranged by size:
3063 2283 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3782 5166 5434 1632
Size: 2150 1384 268 1917
Fragments arranged by size:
2150 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5522
AflIII A'CryG_T
Cuts at: 1203 4618 5595 1203
Size: 3415 977 1327
Fragments arranged by size:
3415 1327 977
AgeI A'CCGG_T
Cuts at: 4550 4550
Size: 5719
AhdI GACnn_n'nnGTC
Cuts at: 2227 4448 2227
Size: 2221 3498
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5719
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5719
AlwNI CAG_nnn'CTG
Cuts at: 3462 5034 3462
Size: 1572 4147
ApaLI G'TGCA_C
Cuts at: 3776 4932 3776
Size: 1156 4563
ApoI r'AATT_y
Cuts at: 1493 2741 3028 3254 3406 5515 1493
Size: 1248 287 226 152 2109 1697
Fragments arranged by size:
2109 1697 1248 287 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4273 160
Size: 872 3241 1606
Fragments arranged by size:
3241 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
AvaI C'yCGr_G
Cuts at: 1870 1943 2175 2361 3044 1870
Size: 73 232 186 683 4545
Fragments arranged by size:
4545 683 232 186 73
BamHI G'GATC_C
Cuts at: 3049 3416 3049
Size: 367 5352
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3040 4495 618
Size: 325 217 627 1253 1455 1842
Fragments arranged by size:
1842 1455 1253 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3038 1391
Size: 1647 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2023 928
Size: 971 124 4624
Fragments arranged by size:
4624 971 124
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2591 2625 3910 3944 1903
Size: 34 654 34 1285 34 3678
Fragments arranged by size:
3678 1285 654 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3611 4821 3611
Size: 1210 4509
BclI T'GATC_A
Cuts at: 1548 2247 1548
Size: 699 5020
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4330 136
Size: 107 122 71 631 605 2658 1525
Fragments arranged by size:
2658 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2547 5714 2547
Size: 3167 2552
BlpI GC'TnA_GC
Cuts at: 3136 3136
Size: 5719
Bme1580I G_kGCm'C
Cuts at: 1790 2073 3780 4936 1790
Size: 283 1707 1156 2573
Fragments arranged by size:
2573 1707 1156 283
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5719
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2032 2266 3450
Size: 416 53 83 399 24 615 234 1184
Cuts at: 3450 4408 442
Size: 958 1753
Fragments arranged by size:
1753 1184 958 615 416 399 234 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2564 2941 2946 4379 1239
Size: 1325 377 5 1433 2579
Fragments arranged by size:
2579 1433 1325 377 5
Bpu10I CC'TnA_GC
Cuts at: 2708 2708
Size: 5719
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3844 4709 5007 3844
Size: 865 298 4556
Fragments arranged by size:
4556 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2559 4382 882
Size: 617 1060 1823 2219
Fragments arranged by size:
2219 1823 1060 617
BsaAI yAC'GTr
Cuts at: 493 1204 3510 493
Size: 711 2306 2702
Fragments arranged by size:
2702 2306 711
BsaBI GATnn'nnATC
Cuts at: 5713 5713
Size: 5719
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3526 3908 4543 275
Size: 53 83 186 936 1993 382 635 1451
Fragments arranged by size:
1993 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4152 4550 4824 4971 5382 4152
Size: 398 274 147 411 4489
Fragments arranged by size:
4489 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2058 2088 2939 2969 1178
Size: 30 165 30 655 30 851 30 3928
Fragments arranged by size:
3928 851 655 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2379 2398 1186
Size: 1193 19 4507
Fragments arranged by size:
4507 1193 19
BseYI C'CCAG_C
Cuts at: 2213 4922 5418 2213
Size: 2709 496 2514
Fragments arranged by size:
2709 2514 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2354 1163
Size: 573 618 4528
Fragments arranged by size:
4528 618 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3169 3930 4958 1540
Size: 275 186 1168 761 1028 2301
Fragments arranged by size:
2301 1168 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2965 3780 3865 4936 2965
Size: 815 85 1071 3748
Fragments arranged by size:
3748 1071 815 85
BsmI GAATG_Cn'
Cuts at: 2591 3230 3323 2591
Size: 639 93 4987
Fragments arranged by size:
4987 639 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2965 3038 3780 3865 4936 1391
Size: 399 283 892 73 742 85 1071 2174
Fragments arranged by size:
2174 1071 892 742 399 283 85 73
BspEI T'CCGG_A
Cuts at: 5382 5382
Size: 5719
BspHI T'CATG_A
Cuts at: 2283 2397 3608 5338 2283
Size: 114 1211 1730 2664
Fragments arranged by size:
2664 1730 1211 114
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2333 3074 844
Size: 1489 741 3489
Fragments arranged by size:
3489 1489 741
BsrBI CCG'CTC
Cuts at: 1394 1823 3606 1394
Size: 429 1783 3507
Fragments arranged by size:
3507 1783 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4214 4388 65
Size: 1183 2966 174 1396
Fragments arranged by size:
2966 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4363 4550 1700
Size: 110 2553 187 2869
Fragments arranged by size:
2869 2553 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5719
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5719
BssSI C'ACGA_G
Cuts at: 2241 3779 4791 2241
Size: 1538 1012 3169
Fragments arranged by size:
3169 1538 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5719
BstBI TT'CG_AA
Cuts at: 4523 4523
Size: 5719
BstEII G'GTnAC_C
Cuts at: 1184 2170 1184
Size: 986 4733
BstXI CCAn_nnnn'nTGG
Cuts at: 2107 2782 2965 2107
Size: 675 183 4861
Fragments arranged by size:
4861 675 183
BstYI r'GATC_y
Cuts at: 1972 2547 3049 3416 3801 3818 5270 5356 5368
Size: 575 502 367 385 17 1452 86 12
Cuts at: 5368 5714 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 575 502 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2147 2147
Size: 5719
BtgI C'CryG_G
Cuts at: 513 1065 2733 513
Size: 552 1668 3499
Fragments arranged by size:
3499 1668 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4980
Fragments arranged by size:
4980 683 56
BtsI GCAGTG_nn'
Cuts at: 2443 3235 4029 4049 2443
Size: 792 794 20 4113
Fragments arranged by size:
4113 794 792 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5719
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5684
DraI TTT'AAA
Cuts at: 3023 3023
Size: 5719
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4726 809
Size: 507 3410 1802
Fragments arranged by size:
3410 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2874 3166 4055
Size: 54 1425 50 275 18 1044 292 889
Cuts at: 4055 5684 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1044 889 292 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3166 1537
Size: 275 1354 4090
Fragments arranged by size:
4090 1354 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2060 3649 1353
Size: 707 1589 3423
Fragments arranged by size:
3423 1589 707
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4306 4680 4826 387
Size: 1064 138 2717 374 146 1280
Fragments arranged by size:
2717 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2564 2941 2946 3782 4379 5166 5434
Size: 393 932 377 5 836 597 787 268
Cuts at: 5434 1239
Size: 1524
Fragments arranged by size:
1524 932 836 787 597 393 377 268
5
EcoO109I rG'GnC_Cy
Cuts at: 2988 2988
Size: 5719
EcoRI G'AATT_C
Cuts at: 2741 3028 3406 2741
Size: 287 378 5054
Fragments arranged by size:
5054 378 287
FspI TGC'GCA
Cuts at: 2078 4225 2078
Size: 2147 3572
HaeII r_GCGC'y
Cuts at: 1678 2142 4866 1678
Size: 464 2724 2531
Fragments arranged by size:
2724 2531 464
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2213 2245 2329 2361 4360
Size: 32 135 32 641 32 84 32 1999
Cuts at: 4360 4392 4434 4466 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1999 641 135 84 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3063 3309 669
Size: 988 281 1125 246 3079
Fragments arranged by size:
3079 1125 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2885 748
Size: 2137 3582
HpaI GTT'AAC
Cuts at: 3309 3309
Size: 5719
KpnI G_GTAC'C
Cuts at: 3044 3044
Size: 5719
MfeI C'AATT_G
Cuts at: 3318 3318
Size: 5719
MluI A'CGCG_T
Cuts at: 5595 5595
Size: 5719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4833 5017 1305
Size: 3528 184 2007
Fragments arranged by size:
3528 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2876 10
Size: 54 1425 343 1044 2853
Fragments arranged by size:
2853 1425 1044 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2420 3677 4036 4195 518
Size: 648 1254 1257 359 159 2042
Fragments arranged by size:
2042 1257 1254 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2082 3135 3812 4960 5205 1606
Size: 476 1053 677 1148 245 2120
Fragments arranged by size:
2120 1148 1053 677 476 245
NcoI C'CATG_G
Cuts at: 513 1065 2733 513
Size: 552 1668 3499
Fragments arranged by size:
3499 1668 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5719
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5719
NotI GC'GGCC_GC
Cuts at: 3166 3166
Size: 5719
NspI r_CATG'y
Cuts at: 1132 1229 2818 3077 4622 1132
Size: 97 1589 259 1545 2229
Fragments arranged by size:
2229 1589 1545 259 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5719
PciI A'CATG_T
Cuts at: 4618 4618
Size: 5719
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2296 1108
Size: 1188 4531
PmeI GTTT'AAAC
Cuts at: 3023 3023
Size: 5719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3788 3820 5301 5333 3788
Size: 32 1481 32 4174
Fragments arranged by size:
4174 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2988 2988
Size: 5719
PshAI GACnn'nnGTC
Cuts at: 1613 2313 2497 1613
Size: 700 184 4835
Fragments arranged by size:
4835 700 184
PsiI TTA'TAA
Cuts at: 3289 3289
Size: 5719
PstI C_TGCA'G
Cuts at: 830 2728 3071 830
Size: 1898 343 3478
Fragments arranged by size:
3478 1898 343
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5719
RsrII CG'GwC_CG
Cuts at: 2122 2122
Size: 5719
SalI G'TCGA_C
Cuts at: 1936 3061 1936
Size: 1125 4594
SanDI GG'GwC_CC
Cuts at: 2988 2988
Size: 5719
SapI GCTCTTCn'nnn_
Cuts at: 2060 2060
Size: 5719
SbfI CC_TGCA'GG
Cuts at: 3071 3071
Size: 5719
ScaI AGT'ACT
Cuts at: 3967 5679 3967
Size: 1712 4007
SexAI A'CCwGG_T
Cuts at: 2765 2765
Size: 5719
SfcI C'TryA_G
Cuts at: 826 1045 2724 3067 4202 4883 5074 826
Size: 219 1679 343 1135 681 191 1471
Fragments arranged by size:
1679 1471 1135 681 343 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5719
SmaI CCC'GGG
Cuts at: 1872 2177 2363 3046 1872
Size: 305 186 683 4545
Fragments arranged by size:
4545 683 305 186
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3823 4724 4986 820
Size: 197 926 1880 901 262 1553
Fragments arranged by size:
1880 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3510 493
Size: 3017 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5719
SphI G_CATG'C
Cuts at: 1132 3077 1132
Size: 1945 3774
SspI AAT'ATT
Cuts at: 5 52 3643 5
Size: 47 3591 2081
Fragments arranged by size:
3591 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5719
StyI C'CwwG_G
Cuts at: 513 1065 2733 3158 513
Size: 552 1668 425 3074
Fragments arranged by size:
3074 1668 552 425
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2681 3746 3763 3916 4101 4546 325
Size: 1255 1101 1065 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1101 1065 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2274 3965 5677
Size: 274 80 33 51 1443 297 1691 1712
Cuts at: 5677 96
Size: 138
Fragments arranged by size:
1712 1691 1443 297 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2058 2090 2424 2456 5679 5711 2058
Size: 32 334 32 3223 32 2066
Fragments arranged by size:
3223 2066 334 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5719
XbaI T'CTAG_A
Cuts at: 3055 3055
Size: 5719
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2484 2484
Size: 5719
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5719
XmaI C'CCGG_G
Cuts at: 1870 2175 2361 3044 1870
Size: 305 186 683 4545
Fragments arranged by size:
4545 683 305 186
XmnI GAAnn'nnTTC
Cuts at: 3848 3848
Size: 5719
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3527 276
Size: 53 83 186 936 1993 2468
Fragments arranged by size:
2468 1993 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII SacI SacII SfiI SfoI SrfI SwaI