Restriction map for
pFN21AB4505
(Circular) (Six-base) MAPSORT of: pFN21AB4505.seq Check: 5321 from: 1 to: 8221
pFN21AB4505 8221 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2458 2559 3074 3254 5125 5266 2458
Size: 101 515 180 1871 141 5413
Fragments arranged by size:
5413 1871 515 180 141 101
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2430 3234 6031 278
Size: 53 83 186 936 894 804 2797 2468
Fragments arranged by size:
2797 2468 936 894 804 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4458 5564 1937
Size: 2521 1106 4594
Fragments arranged by size:
4594 2521 1106
Acc65I G'GTAC_C
Cuts at: 5542 5542
Size: 8221
AclI AA'CG_TT
Cuts at: 1563 6348 6721 1563
Size: 4785 373 3063
Fragments arranged by size:
4785 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2593 2606 4000 4864 5017 6284 7668 7936
Size: 961 13 1394 864 153 1267 1384 268
Cuts at: 7936 1632
Size: 1917
Fragments arranged by size:
1917 1394 1384 1267 961 864 268 153
13
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8024
AflIII A'CryG_T
Cuts at: 1203 2952 3023 3191 7120 8097 1203
Size: 1749 71 168 3929 977 1327
Fragments arranged by size:
3929 1749 1327 977 168 71
AgeI A'CCGG_T
Cuts at: 2197 7052 2197
Size: 4855 3366
AhdI GACnn_n'nnGTC
Cuts at: 2287 6950 2287
Size: 4663 3558
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8221
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4374 1184
Size: 3190 5031
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2963 2995 2963
Size: 32 8189
AlwNI CAG_nnn'CTG
Cuts at: 5964 7536 5964
Size: 1572 6649
ApaI G_GGCC'C
Cuts at: 2148 2321 2774 3258 2148
Size: 173 453 484 7111
Fragments arranged by size:
7111 484 453 173
ApaLI G'TGCA_C
Cuts at: 4349 4526 6278 7434 4349
Size: 177 1752 1156 5136
Fragments arranged by size:
5136 1752 1156 177
ApoI r'AATT_y
Cuts at: 1493 3038 4177 5530 5756 5908 8017 1493
Size: 1545 1139 1353 226 152 2109 1697
Fragments arranged by size:
2109 1697 1545 1353 1139 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6775 160
Size: 872 5743 1606
Fragments arranged by size:
5743 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8221
AvaI C'yCGr_G
Cuts at: 1870 1943 2792 2845 3558 3682 5546 1870
Size: 73 849 53 713 124 1864 4545
Fragments arranged by size:
4545 1864 849 713 124 73 53
BamHI G'GATC_C
Cuts at: 3543 4906 5551 5918 3543
Size: 1363 645 367 5846
Fragments arranged by size:
5846 1363 645 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2126 2399 3467 3758 3823
Size: 325 217 627 339 273 1068 291 65
Cuts at: 3823 5340 5542 6997 618
Size: 1517 202 1455 1842
Fragments arranged by size:
1842 1517 1455 1068 627 339 325 291
273 217 202 65
BanII G_rGCy'C
Cuts at: 1391 2148 2321 2538 2774 2844 2853 2925 3258
Size: 757 173 217 236 70 9 72 333
Cuts at: 3258 3500 3691 3948 4071 5540 1391
Size: 242 191 257 123 1469 4072
Fragments arranged by size:
4072 1469 757 333 257 242 236 217
191 173 123 72 70 9
BbeI G_GCGC'C
Cuts at: 2130 3471 2130
Size: 1341 6880
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3336 4197 4420 5458 928
Size: 971 1437 861 223 1038 3691
Fragments arranged by size:
3691 1437 1038 971 861 223
BbvCI CC'TCA_GC
Cuts at: 2216 2216
Size: 8221
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5057 5091 6412 6446 1903
Size: 34 3120 34 1321 34 3678
Fragments arranged by size:
3678 3120 1321 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6113 7323 6113
Size: 1210 7011
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8221
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2688 3741 4050
Size: 107 122 71 631 605 1016 1053 309
Cuts at: 4050 4655 4956 6832 136
Size: 605 301 1876 1525
Fragments arranged by size:
1876 1525 1053 1016 631 605 605 309
301 122 107 71
BglII A'GATC_T
Cuts at: 8216 8216
Size: 8221
BlpI GC'TnA_GC
Cuts at: 2873 5638 2873
Size: 2765 5456
Bme1580I G_kGCm'C
Cuts at: 1790 2148 2300 2321 2774 3258 3411 3790 4353
Size: 358 152 21 453 484 153 379 563
Cuts at: 4353 4530 6282 7438 1790
Size: 177 1752 1156 2573
Fragments arranged by size:
2573 1752 1156 563 484 453 379 358
177 153 152 21
BmgBI CAC'GTC
Cuts at: 1318 2857 3192 4549 1318
Size: 1539 335 1357 4990
Fragments arranged by size:
4990 1539 1357 335
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3172 3398 3685
Size: 416 53 83 399 24 1755 226 287
Cuts at: 3685 4948 5161 5952 6910 442
Size: 1263 213 791 958 1753
Fragments arranged by size:
1755 1753 1263 958 791 416 399 287
226 213 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8221
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 8221
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2032 2333 2441 3343 3514 6881 1239
Size: 793 301 108 902 171 3367 2579
Fragments arranged by size:
3367 2579 902 793 301 171 108
Bpu10I CC'TnA_GC
Cuts at: 2216 3576 2216
Size: 1360 6861
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2258 2870 5183 5457 5483 6346 7211 7509 2258
Size: 612 2313 274 26 863 865 298 2970
Fragments arranged by size:
2970 2313 865 863 612 298 274 26
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2330 2419 2474 3162 3443 6884 882
Size: 617 831 89 55 688 281 3441 2219
Fragments arranged by size:
3441 2219 831 688 617 281 89 55
BsaAI yAC'GTr
Cuts at: 493 1204 2497 2578 2767 3024 4348 5050 6012
Size: 711 1293 81 189 257 1324 702 962
Cuts at: 6012 493
Size: 2702
Fragments arranged by size:
2702 1324 1293 962 711 702 257 189
81
BsaBI GATnn'nnATC
Cuts at: 8215 8215
Size: 8221
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2127 2427 3231 3468
Size: 53 83 186 936 594 300 804 237
Cuts at: 3468 4395 4686 4716 6028 6410 7045 275
Size: 927 291 30 1312 382 635 1451
Fragments arranged by size:
1451 1312 936 927 804 635 594 382
300 291 237 186 83 53 30
BsaWI w'CCGG_w
Cuts at: 2197 6654 7052 7326 7473 7884 2197
Size: 4457 398 274 147 411 2534
Fragments arranged by size:
4457 2534 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3140 3170 4385 4415 1178
Size: 30 165 30 1737 30 1215 30 4984
Fragments arranged by size:
4984 1737 1215 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2800 2831 3161 3655 4268 1186
Size: 1614 31 330 494 613 5139
Fragments arranged by size:
5139 1614 613 494 330 31
BseYI C'CCAG_C
Cuts at: 2045 2268 2774 2821 3207 5452 7424 7920 2045
Size: 223 506 47 386 2245 1972 496 2346
Fragments arranged by size:
2346 2245 1972 506 496 386 223 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2486 3001 3505 3635 3715 1163
Size: 573 750 515 504 130 80 5669
Fragments arranged by size:
5669 750 573 515 504 130 80
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5671 6432 7460 1540
Size: 275 186 3670 761 1028 2301
Fragments arranged by size:
3670 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2550 2844 2925 3185 4353 4530 5235 6282 6367
Size: 294 81 260 1168 177 705 1047 85
Cuts at: 6367 7438 2550
Size: 1071 3333
Fragments arranged by size:
3333 1168 1071 1047 705 294 260 177
85 81
BsmI GAATG_Cn'
Cuts at: 3116 5513 5732 5825 3116
Size: 2397 219 93 5512
Fragments arranged by size:
5512 2397 219 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2356 2723 3395 4665 2356
Size: 367 672 1270 5912
Fragments arranged by size:
5912 1270 672 367
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2148 2300 2321 2538 2550 2774 2844
Size: 399 358 152 21 217 12 224 70
Cuts at: 2844 2853 2925 3185 3258 3411 3500 3691 3790
Size: 9 72 260 73 153 89 191 99
Cuts at: 3790 3948 4071 4353 4530 5235 5540 6282 6367
Size: 158 123 282 177 705 305 742 85
Cuts at: 6367 7438 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 705 399 358 305 282
260 224 217 191 177 158 153 152
123 99 89 85 73 72 70 21
12 9
BspEI T'CCGG_A
Cuts at: 7884 7884
Size: 8221
BspHI T'CATG_A
Cuts at: 6110 7840 6110
Size: 1730 6491
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2458 2559 3038 3074 3089 3254 5125 5266
Size: 1614 101 479 36 15 165 1871 141
Cuts at: 5266 5576 844
Size: 310 3489
Fragments arranged by size:
3489 1871 1614 479 310 165 141 101
36 15
BsrBI CCG'CTC
Cuts at: 1394 1823 6108 1394
Size: 429 4285 3507
Fragments arranged by size:
4285 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3760 6716 6890 65
Size: 1183 2512 2956 174 1396
Fragments arranged by size:
2956 2512 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2161 2197 2976 3219 3536 4740 5078
Size: 110 351 36 779 243 317 1204 338
Cuts at: 5078 6865 7052 1700
Size: 1787 187 2869
Fragments arranged by size:
2869 1787 1204 779 351 338 317 243
187 110 36
BsrGI T'GTAC_A
Cuts at: 96 4029 96
Size: 3933 4288
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8221
BssSI C'ACGA_G
Cuts at: 2809 2861 6281 7293 2809
Size: 52 3420 1012 3737
Fragments arranged by size:
3737 3420 1012 52
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3246 3429 794
Size: 2452 183 5586
Fragments arranged by size:
5586 2452 183
BstBI TT'CG_AA
Cuts at: 7025 7025
Size: 8221
BstEII G'GTnAC_C
Cuts at: 1184 2060 3333 5093 1184
Size: 876 1273 1760 4312
Fragments arranged by size:
4312 1760 1273 876
BstYI r'GATC_y
Cuts at: 1972 2377 3543 4906 5551 5918 6303 6320 7772
Size: 405 1166 1363 645 367 385 17 1452
Cuts at: 7772 7858 7870 8216 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1363 1166 645 405 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5237 5237
Size: 8221
BtgI C'CryG_G
Cuts at: 513 1065 2004 2140 2364 3161 3509 3651 4404
Size: 552 939 136 224 797 348 142 753
Cuts at: 4404 5217 513
Size: 813 3517
Fragments arranged by size:
3517 939 813 797 753 552 348 224
142 136
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4230 4981 487
Size: 683 56 3004 751 3727
Fragments arranged by size:
3727 3004 751 683 56
BtsI GCAGTG_nn'
Cuts at: 2453 3856 4144 5737 6531 6551 2453
Size: 1403 288 1593 794 20 4123
Fragments arranged by size:
4123 1593 1403 794 288 20
ClaI AT'CG_AT
Cuts at: 1554 3483 1554
Size: 1929 6292
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3421 3456 4909 4944 529
Size: 35 2857 35 1453 35 3806
Fragments arranged by size:
3806 2857 1453 35 35 35
DraI TTT'AAA
Cuts at: 3043 5525 3043
Size: 2482 5739
DraIII CAC_nnn'GTG
Cuts at: 2638 3024 3136 2638
Size: 386 112 7723
Fragments arranged by size:
7723 386 112
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7228 809
Size: 507 5912 1802
Fragments arranged by size:
5912 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2580 2974 3442
Size: 54 1425 50 275 18 750 394 468
Cuts at: 3442 3487 4606 4675 5668 6557 8186 8
Size: 45 1119 69 993 889 1629 43
Fragments arranged by size:
1629 1425 1119 993 889 750 468 394
275 69 54 50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5668 1537
Size: 275 3856 4090
Fragments arranged by size:
4090 3856 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4286 6151 1353
Size: 2933 1865 3423
Fragments arranged by size:
3423 2933 1865
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2573 6808 7182 7328 387
Size: 1064 138 984 4235 374 146 1280
Fragments arranged by size:
4235 1280 1064 984 374 146 138
EcoICRI GAG'CTC
Cuts at: 2842 2923 2842
Size: 81 8140
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2032 2333 2441 2593 2606 3343 3514
Size: 393 400 301 108 152 13 737 171
Cuts at: 3514 4000 4864 5017 6284 6881 7668 7936 1239
Size: 486 864 153 1267 597 787 268 1524
Fragments arranged by size:
1524 1267 864 787 737 597 486 400
393 301 268 171 153 152 108 13
EcoO109I rG'GnC_Cy
Cuts at: 2317 2318 2771 3204 3254 3255 3714 3772 5417
Size: 1 453 433 50 1 459 58 1645
Cuts at: 5417 2317
Size: 5121
Fragments arranged by size:
5121 1645 459 453 433 58 50 1
1
EcoRI G'AATT_C
Cuts at: 5530 5908 5530
Size: 378 7843
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2269 2269
Size: 8221
FspI TGC'GCA
Cuts at: 6727 6727
Size: 8221
HaeII r_GCGC'y
Cuts at: 1678 2130 3471 7368 1678
Size: 452 1341 3897 2531
Fragments arranged by size:
3897 2531 1341 452
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2273 2305 2688 2720 3140
Size: 32 135 32 701 32 383 32 420
Cuts at: 3140 3172 4385 4417 5006 5038 6862 6894 6936
Size: 32 1213 32 589 32 1824 32 42
Cuts at: 6936 6968 1373
Size: 32 2626
Fragments arranged by size:
2626 1824 1213 701 589 420 383 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3838 5565 5811 669
Size: 988 281 1900 1727 246 3079
Fragments arranged by size:
3079 1900 1727 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8221
HpaI GTT'AAC
Cuts at: 5811 5811
Size: 8221
KasI G'GCGC_C
Cuts at: 2126 3467 2126
Size: 1341 6880
KpnI G_GTAC'C
Cuts at: 5546 5546
Size: 8221
MfeI C'AATT_G
Cuts at: 5820 5820
Size: 8221
MluI A'CGCG_T
Cuts at: 8097 8097
Size: 8221
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2379 3693 4668 7335 7519 1305
Size: 1074 1314 975 2667 184 2007
Fragments arranged by size:
2667 2007 1314 1074 975 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2582 10
Size: 54 1425 343 750 5649
Fragments arranged by size:
5649 1425 750 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 6179 6538 6697 518
Size: 648 5013 359 159 2042
Fragments arranged by size:
5013 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2142 2366 3015 3492 4329 5637 6314 7462
Size: 536 224 649 477 837 1308 677 1148
Cuts at: 7462 7707 1606
Size: 245 2120
Fragments arranged by size:
2120 1308 1148 837 677 649 536 477
245 224
NaeI GCC'GGC
Cuts at: 2163 4742 5080 2163
Size: 2579 338 5304
Fragments arranged by size:
5304 2579 338
NarI GG'CG_CC
Cuts at: 2127 3468 2127
Size: 1341 6880
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6730
Fragments arranged by size:
6730 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8221
NgoMIV G'CCGG_C
Cuts at: 2161 4740 5078 2161
Size: 2579 338 5304
Fragments arranged by size:
5304 2579 338
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8221
NotI GC'GGCC_GC
Cuts at: 5668 5668
Size: 8221
NspI r_CATG'y
Cuts at: 1132 1229 2411 2753 2956 5579 7124 1132
Size: 97 1182 342 203 2623 1545 2229
Fragments arranged by size:
2623 2229 1545 1182 342 203 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8221
PciI A'CATG_T
Cuts at: 2952 7120 2952
Size: 4168 4053
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 3136 3485 4181 1108
Size: 902 1126 349 696 5148
Fragments arranged by size:
5148 1126 902 696 349
PfoI T'CCnGG_A
Cuts at: 2835 3546 2835
Size: 711 7510
PmeI GTTT'AAAC
Cuts at: 5525 5525
Size: 8221
PmlI CAC'GTG
Cuts at: 2497 3024 2497
Size: 527 7694
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6290 6322 7803 7835 6290
Size: 32 1481 32 6676
Fragments arranged by size:
6676 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3204 3714 3772 5417 3204
Size: 510 58 1645 6008
Fragments arranged by size:
6008 1645 510 58
PshAI GACnn'nnGTC
Cuts at: 1613 4446 1613
Size: 2833 5388
PsiI TTA'TAA
Cuts at: 5791 5791
Size: 8221
PspOMI G'GGCC_C
Cuts at: 2144 2317 2770 3254 2144
Size: 173 453 484 7111
Fragments arranged by size:
7111 484 453 173
PstI C_TGCA'G
Cuts at: 830 2556 5573 830
Size: 1726 3017 3478
Fragments arranged by size:
3478 3017 1726
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8221
PvuII CAG'CTG
Cuts at: 3015 3015
Size: 8221
SacI G_AGCT'C
Cuts at: 2844 2925 2844
Size: 81 8140
SacII CC_GC'GG
Cuts at: 2143 2367 2143
Size: 224 7997
SalI G'TCGA_C
Cuts at: 1936 5563 1936
Size: 3627 4594
SanDI GG'GwC_CC
Cuts at: 3204 3204
Size: 8221
SbfI CC_TGCA'GG
Cuts at: 2556 5573 2556
Size: 3017 5204
ScaI AGT'ACT
Cuts at: 6469 8181 6469
Size: 1712 6509
SfcI C'TryA_G
Cuts at: 826 1045 2485 2552 5001 5332 5569 6704 7385
Size: 219 1440 67 2449 331 237 1135 681
Cuts at: 7385 7576 826
Size: 191 1471
Fragments arranged by size:
2449 1471 1440 1135 681 331 237 219
191 67
SfoI GGC'GCC
Cuts at: 2128 3469 2128
Size: 1341 6880
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8221
SgrAI Cr'CCGG_yG
Cuts at: 1700 2197 1700
Size: 497 7724
SmaI CCC'GGG
Cuts at: 1872 3560 3684 5548 1872
Size: 1688 124 1864 4545
Fragments arranged by size:
4545 1864 1688 124
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2273 2885 5198 5472 5498 6325
Size: 197 926 330 612 2313 274 26 827
Cuts at: 6325 7226 7488 820
Size: 901 262 1553
Fragments arranged by size:
2313 1553 926 901 827 612 330 274
262 197 26
SnaBI TAC'GTA
Cuts at: 493 5050 6012 493
Size: 4557 962 2702
Fragments arranged by size:
4557 2702 962
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8221
SphI G_CATG'C
Cuts at: 1132 5579 1132
Size: 4447 3774
SspI AAT'ATT
Cuts at: 5 52 6145 5
Size: 47 6093 2081
Fragments arranged by size:
6093 2081 47
StuI AGG'CCT
Cuts at: 1517 3599 1517
Size: 2082 6139
StyI C'CwwG_G
Cuts at: 513 1065 2004 3338 4902 5660 513
Size: 552 939 1334 1564 758 3074
Fragments arranged by size:
3074 1564 1334 939 758 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2026 2992 3776 4268 4559 4937 6248
Size: 1255 446 966 784 492 291 378 1311
Cuts at: 6248 6265 6418 6603 7048 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1311 1255 966 784 492 446 445
378 291 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4029 6467 8179
Size: 274 80 33 51 1443 2052 2438 1712
Cuts at: 8179 96
Size: 138
Fragments arranged by size:
2438 2052 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8181 8213 8181
Size: 32 8189
Tth111I GACn'n_nGTC
Cuts at: 1316 4424 1316
Size: 3108 5113
XbaI T'CTAG_A
Cuts at: 5557 5557
Size: 8221
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2764 4114 5438 2764
Size: 1350 1324 5547
Fragments arranged by size:
5547 1350 1324
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8221
XmaI C'CCGG_G
Cuts at: 1870 3558 3682 5546 1870
Size: 1688 124 1864 4545
Fragments arranged by size:
4545 1864 1688 124
XmnI GAAnn'nnTTC
Cuts at: 2661 6350 2661
Size: 3689 4532
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2428 3232 6029 276
Size: 53 83 186 936 894 804 2797 2468
Fragments arranged by size:
2797 2468 936 894 804 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I
EcoNI EcoRV FseI FspAI NruI NsiI PacI PspXI
PsrI RsrII SapI SexAI SfiI SrfI SwaI